Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,200,251 G→T 6.0% H239N (CAT→AAT)  ygiF ← inorganic triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,200,2510GT 6.0% 239.7 / 2.7 66H239N (CAT→AAT) ygiFinorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base T (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCC  >  NC_000913/3200116‑3200390
                                                                                                                                       |                                                                                                                                            
gcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑c                                                                                                                                         <  1:444014/140‑1 (MQ=255)
gcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑c                                                                                                                                         <  1:140674/140‑1 (MQ=255)
 cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑Ct                                                                                                                                        >  2:893476/1‑140 (MQ=255)
 cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAAAACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCAGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑Ct                                                                                                                                        >  2:893465/1‑140 (MQ=255)
    aTCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGcc                                                                                                                                     >  2:245354/1‑140 (MQ=255)
    aTCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCAGCAGCGTAGGACGGACCAGGCTAATGGCTGCCAGCACCTGTGCTTTCGCCGCATCGTTGCCGCGTACCCACCGTTCTTCATGATA‑CTGcc                                                                                                                                     >  2:1144800/1‑140 (MQ=255)
      cACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAt                                                                                                                                   >  1:425015/1‑140 (MQ=255)
      cACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAt                                                                                                                                   <  1:1117181/140‑1 (MQ=255)
       aCGTAAGTGAGTGCTCGCTTTACGCTGCACAATACCAACGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGGATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAtt                                                                                                                                  <  1:564792/140‑1 (MQ=255)
       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAtt                                                                                                                                  >  1:562146/1‑140 (MQ=255)
       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAtt                                                                                                                                  <  2:78903/140‑1 (MQ=255)
       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCAtt                                                                                                                                  <  2:966529/140‑1 (MQ=255)
                agtgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACg                                                                                                                         >  1:793798/1‑140 (MQ=255)
                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGcc                                                                                                                       >  2:553961/1‑140 (MQ=255)
                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGcc                                                                                                                       <  1:66209/140‑1 (MQ=255)
                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGcc                                                                                                                       <  1:793165/140‑1 (MQ=255)
                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGcc                                                                                                                       >  2:985166/1‑140 (MQ=255)
                  tgCTCGCTATGCGCGGCACTATCCCACAGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGGCAGCACCTGTGCTGTCGGCGCTTCGTTGCCGCGTCGCCACTGTTCTTCTGGAGA‑CTGCCAGTTCGCTTACGcg                                                                                                                       >  2:424386/1‑139 (MQ=255)
                   gCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCa                                                                                                                      >  1:808472/1‑140 (MQ=255)
                        cTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCg                                                                                                                 >  1:722085/1‑140 (MQ=255)
                          ttACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAg                                                                                                               >  2:153773/1‑140 (MQ=255)
                          ttACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAg                                                                                                               >  2:153984/1‑140 (MQ=255)
                             cgcgGCACAATACCACCGAACAGCAACACCCTATCACTGACCAGGCTAATCGCTGCCAGCACCTGTACTTTCGCCGCAACGTTGCCGCGAACCCACATTACTTCAAGATA‑CTGCCATTCCGCTAACGCCAGCTCGAccgc                                                                                                            <  2:595171/140‑1 (MQ=255)
                                  cacaATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCtt                                                                                                       >  1:1071149/1‑140 (MQ=255)
                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                                                                             >  1:1103816/1‑140 (MQ=255)
                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                                                                             >  1:933644/1‑140 (MQ=255)
                                               aaCAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTc                                                                                          >  2:557775/1‑140 (MQ=255)
                                               aaCAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTc                                                                                          >  2:557689/1‑140 (MQ=255)
                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATTATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                                                                        >  2:176244/1‑140 (MQ=255)
                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATTATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                                                                        >  2:176056/1‑140 (MQ=255)
                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                                                                        >  2:173976/1‑140 (MQ=255)
                                                   gCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCcaca                                                                                      <  2:481495/140‑1 (MQ=255)
                                                    cATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACAt                                                                                     <  2:456156/140‑1 (MQ=255)
                                                                    aCCGGGCTAGTGGCTGGCAGCACGTGTTCTTTCGCCGCATGGTTGCCGGGTGCCCCCCGTTCTTCATGATA‑GTGCCATTGCGCTTCCGCCAGCTCGAGCGCCGCTTCCAGGCCCTGTTCCTGTAGGGCTTTTGCCGCAAc                                                                     >  2:1044461/1‑140 (MQ=255)
                                                                    aCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGcttc                                                                     <  1:692775/140‑4 (MQ=255)
                                                                    aCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                                                                     <  1:692625/140‑1 (MQ=255)
                                                                    aCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                                                                     >  1:787185/1‑140 (MQ=255)
                                                                       aGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATg                                                                  <  1:1090111/140‑1 (MQ=255)
                                                                        cgCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGAGCGTC‑TGGTACCAGCCATTGCGCTAACGCCAGCTCGAGCGCCGCGACCAGCCCCTGTTAGACCTCGGCGTTTGCCGCatgctgc                                                                 >  2:383981/2‑134 (MQ=255)
                                                                              tGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtg                                                           >  1:1048606/1‑140 (MQ=255)
                                                                                  tGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg                                                       >  1:12221/1‑140 (MQ=255)
                                                                                  tGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg                                                       >  1:12252/1‑140 (MQ=255)
                                                                                  tGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg                                                       >  1:921702/1‑140 (MQ=255)
                                                                                  tGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg                                                       >  1:920730/1‑140 (MQ=255)
                                                                                   gCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt                                                      <  1:94629/140‑1 (MQ=255)
                                                                                   gCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt                                                      >  1:237096/1‑140 (MQ=255)
                                                                                   gCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGGTCTTCATGATA‑CTGCCATTGCGCTCACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACAGCGGCTTTTGCCGCACCATGCAAAAtggtggg                                                      >  2:686436/1‑139 (MQ=255)
                                                                                    ccAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTc                                                     >  2:829381/1‑140 (MQ=255)
                                                                                       gCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGt                                                  <  2:144443/140‑1 (MQ=255)
                                                                                                    cgccgcATCGTTGCCGCGTACCCCCCGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                     <  1:681617/140‑1 (MQ=255)
                                                                                                    cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                     <  1:874402/140‑1 (MQ=255)
                                                                                                    cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                     <  1:1109139/140‑1 (MQ=255)
                                                                                                    cgccgcATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                     <  1:1224233/140‑1 (MQ=255)
                                                                                                    cgccgcATAGTTGCCGCGTACCCACAGTTCTTCTTGATA‑CTGCCATTGCGATAACGCAAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCAcgcg                                     <  1:1224223/140‑1 (MQ=255)
                                                                                                        gcATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGccag                                 >  1:696870/1‑138 (MQ=255)
                                                                                                              ttGCCGCGTACCCACAGTTCTTCATTATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGcc                           <  1:176056/140‑1 (MQ=255)
                                                                                                              ttGCCGCGTACCCACAGTTCTTCATTATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGcc                           <  1:176244/140‑1 (MQ=255)
                                                                                                              ttGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTTTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGcc                           <  2:837256/140‑1 (MQ=255)
                                                                                                              ttGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGcc                           <  1:834694/140‑1 (MQ=255)
                                                                                                                   gcgTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTgcg                      <  2:237096/140‑1 (MQ=255)
                                                                                                                   gcgTACCCACAGTTCTACATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTATGCCGCAACATGCAAAATGGTGGTCGGTTAGATTTCACGCGCCGGATTGCCCTgcg                      >  2:945052/1‑140 (MQ=255)
                                                                                                                      tACGCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCACAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCa                   <  2:1052896/140‑1 (MQ=255)
                                                                                                                      tACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCa                   <  2:1052857/140‑1 (MQ=255)
                                                                                                                               ttcttcATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGc          >  1:526466/1‑140 (MQ=255)
                                                                                                                                     aTGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCAcgcg    <  1:1165085/140‑1 (MQ=255)
                                                                                                                                     aTGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCAAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGACAGATGATAGCCAcgcg    <  1:468616/140‑1 (MQ=255)
                                                                                                                                       gATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACAGCGGCTATTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGAGGATAGCCACGcgcc  >  2:344791/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                            
GCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATA‑CTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCC  >  NC_000913/3200116‑3200390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑