Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,258,115	G→T	6.8%	intergenic (‑105/+170)	yhaO ← / ← tdcG	putative transporter/L‑serine dehydratase 3, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,258,115	0	G	T	6.8%	224.5 / 3.0	67	intergenic (‑105/+170)	yhaO/tdcG	putative transporter/L‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (40/22);  new base T (2/3);  total (42/25)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCA  >  NC_000913/3257976‑3258248                                                                                                                                            |                                                                                                                                      tCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATg                                                                                                                                       >  1:1001429/1‑140 (MQ=255) tCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATGTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTAGCAGGCGAGTTATTAGAATAAAGAAATg                                                                                                                                       >  1:786245/1‑140 (MQ=255)   tGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGAt                                                                                                                                     >  1:67437/1‑140 (MQ=255)   tGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGAt                                                                                                                                     >  1:808959/1‑140 (MQ=255)          aCGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCTATATTTTGTAGGGCTTATTATTTGTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGAAGGCGAGTTATTAGAATAAAGAAATGATAcgcgcg                                                                                                                              >  1:826398/1‑140 (MQ=255)          aCGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATTATAcgcgcg                                                                                                                              >  2:743949/1‑140 (MQ=255)          aCGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATTATAcgcgcg                                                                                                                              >  2:743920/1‑140 (MQ=255)               tttATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTc                                                                                                                         >  1:825374/1‑140 (MQ=255)                  aTTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCaga                                                                                                                      >  1:20135/1‑140 (MQ=255)                  aTTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCaga                                                                                                                      >  1:776045/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGTCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTATTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:546513/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTCGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:546582/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:63452/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:550550/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:428747/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:765830/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:2179/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:1202586/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAAt                                                                                                                >  2:511621/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGAGGTCTACGTTTTTGCAGGCGAGTAATTAGAATAAAGAAATGAAACGCGCGAGGTCAGAGAAAAt                                                                                                                >  1:848669/1‑140 (MQ=255)                        tGCAATTTCCATAATTTCTCGCTCAATATGTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGACTAAAGAAATGATACGCGCGAGGTCAGTGaaact                                                                                                                >  1:2242/1‑138 (MQ=255)                                                 atatTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATaa                                                                                       <  1:1212064/140‑1 (MQ=255)                                                      ttGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGgag                                                                                  <  1:1012215/140‑1 (MQ=255)                                                       tGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGgaga                                                                                 >  1:829515/1‑140 (MQ=255)                                                            ggCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGAt                                                                            >  1:263250/1‑140 (MQ=255)                                                            ggCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGAt                                                                            >  1:341145/1‑140 (MQ=255)                                                              cTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATaa                                                                          >  1:392353/1‑140 (MQ=255)                                                                           tCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCCCAATCAAGACTTTGAAATAACGGAGATGGAGAAGAATTTTctacta                                                             >  2:122262/1‑140 (MQ=255)                                                                             cGAGCCGCATCCAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           <  2:51256/140‑1 (MQ=255)                                                                             cGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           <  2:51279/140‑1 (MQ=255)                                                                             cGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           <  2:184656/140‑1 (MQ=255)                                                                             cGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCCTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           <  2:513421/140‑1 (MQ=255)                                                                                  cGCATCACGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGAGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATCACGGAGATGGATAAGAATTTACTACTAAATTAAt                                                      <  2:414109/140‑1 (MQ=255)                                                                                    cATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCg                                                    <  2:1157186/140‑1 (MQ=255)                                                                                    cATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCg                                                    <  2:678616/140‑1 (MQ=255)                                                                                          ggCGATATGGGGTCTACGTTTTTGCAGGCGATTTATTAGAATAAAGAAATGTTTCGCGCGAGGTCAGAGAAATTCTTCACAATCAAGACTTTGTAATAACGGAGATGGATAAGAATTTTCTACTTAATTAATCGCAGgag                                              <  2:650141/140‑1 (MQ=255)                                                                                            cGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTCGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGaa                                            <  1:1112147/140‑1 (MQ=255)                                                                                              atatGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAata                                          >  2:35477/1‑140 (MQ=255)                                                                                              atatGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAata                                          >  2:35498/1‑140 (MQ=255)                                                                                               tatGCGGTGTACGTTTTTGCTGGCGAGTTATTAGAATAAAGAAATGATACGCGCGATGTCAGAGAAATTGTTCACAATCAATACTTTGAAATAACGGAGATGGATAAGAATTTTCTAGTAAATTAATCGAAGGAGTatat                                         >  1:624030/1‑140 (MQ=255)                                                                                               tatGCGGTCTTCGTTTTTGCAGGCGAGTTATTAGAATAAAGTAATGATACTCGCGAGGTCAGTGAAAATCTTCTCAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTAGTAAATTATTCGATGTAGAatat                                         >  2:1060205/1‑140 (MQ=255)                                                                                               tatGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGTGGTCAGAGAAAATCTTCACAATCAAGTCTTTGTAATAACGGTGATGGATAAGATTTTTCTACTAAATTAATCGATGTAGAatat                                         >  2:1062365/1‑140 (MQ=255)                                                                                               tatGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAatat                                         >  1:1059653/1‑140 (MQ=255)                                                                                                    ggTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           >  2:733719/1‑117 (MQ=255)                                                                                                    ggTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTaaa                                                           <  1:733719/117‑1 (MQ=255)                                                                                                    ggTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATattcatt                                    <  2:231002/140‑1 (MQ=255)                                                                                                    ggTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATattcatt                                    <  2:231014/140‑1 (MQ=255)                                                                                                     gTCTTCGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAGATTAATCGCAGGAGAATattcattc                                   >  1:242425/1‑140 (MQ=255)                                                                                                     gTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAATAATTTTCTACTAAATTAATCGCAGGAGAATattcattc                                   >  2:1032719/1‑140 (MQ=255)                                                                                                     gTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATattcattc                                   >  1:1045811/1‑140 (MQ=255)                                                                                                     gTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATattcattc                                   >  1:1087038/1‑140 (MQ=255)                                                                                                       cTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATtctc                                 >  1:265625/1‑140 (MQ=255)                                                                                                           gTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTAtt                             >  2:1182815/1‑140 (MQ=255)                                                                                                           gTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTAtt                             >  2:1183446/1‑140 (MQ=255)                                                                                                             ttttGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTg                           <  2:1202586/140‑1 (MQ=255)                                                                                                                tGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCgg                        >  2:382046/1‑140 (MQ=255)                                                                                                                tGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTGGACATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATGCATTCTCTATTTGCgg                        >  1:503801/1‑140 (MQ=255)                                                                                                                     gCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGAt                   >  2:777267/1‑140 (MQ=255)                                                                                                                       gAGTTATTAGAATAAAGAAATTATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATca                 <  1:743949/140‑1 (MQ=255)                                                                                                                       gAGTTATTAGAATAAAGAAATTATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATca                 <  1:743920/140‑1 (MQ=255)                                                                                                                       gAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATca                 <  2:332235/140‑1 (MQ=255)                                                                                                                       gAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATca                 <  2:332182/140‑1 (MQ=255)                                                                                                                       gAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATca                 <  1:592877/140‑1 (MQ=255)                                                                                                                               ataataaataAATTATACGCTCGAGGTCATATAAAATCTTCACAATCAATACTTTGAAATAACTGATATTGATAATAATTTTCTACTAAATTAATCTAATTATAATATTAATTATATATTTGCGGCAGATCACaaaaaaa         <  1:176871/138‑1 (MQ=255)                                                                                                                               aGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTAACAATCAAGAATTTGAAATAACGTAGATGGAAAAGAATTTTCTACTAAATTAATCGCAGGAGAATATACATTCTCTATTTGCGGCAGATCACaaaaaaa         <  2:612666/140‑1 (MQ=255)                                                                                                                                    aaaGAAATGATACGCGCGAGGTCAGAGAATATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTg    <  1:829633/140‑1 (MQ=255)                                                                                                                                    aaaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTg    <  1:787258/140‑1 (MQ=255)                                                                                                                                      aGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGca  >  2:1142644/1‑140 (MQ=255)                                                                                                                                      aGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGca  <  1:950675/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      TCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTATTTTTTTCCGAGCCGCATCAAGGCGATATGCGGTCTACGTTTTTGCAGGCGAGTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCA  >  NC_000913/3257976‑3258248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑