Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,274,579 C→A 7.2% W110L (TGG→TTG)  garP ← putative (D)‑galactarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,274,5790CA 7.2% 189.0 / 3.1 56W110L (TGG→TTG) garPputative (D)‑galactarate transporter
Reads supporting (aligned to +/- strand):  ref base C (23/29);  new base A (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGATGGCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAGGCC  >  NC_000913/3274451‑3274716
                                                                                                                                |                                                                                                                                         
aGATGGCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCggg                                                                                                                                <  1:211143/140‑1 (MQ=255)
aGATGGCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCggg                                                                                                                                <  1:211417/140‑1 (MQ=255)
  aTGGCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGaa                                                                                                                              <  1:204136/140‑1 (MQ=255)
    ggCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACa                                                                                                                            >  1:966966/1‑140 (MQ=255)
     gCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACat                                                                                                                           <  1:855387/140‑1 (MQ=255)
     gCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACat                                                                                                                           <  1:547008/140‑1 (MQ=255)
                 ccACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGcc                                                                                                               <  2:392156/140‑1 (MQ=255)
                    cGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTg                                                                                                            >  2:931150/1‑140 (MQ=255)
                    cGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCAAGGCCAGCGGGAACATATCAACAAAGCCTTg                                                                                                            >  2:613555/1‑140 (MQ=255)
                    cGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCAAGGCCAGCGGGAACATATCAACAAAGCCTTg                                                                                                            >  2:611858/1‑140 (MQ=255)
                     gTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCAGAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGc                                                                                                           >  2:587719/1‑140 (MQ=255)
                      ttCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCAAGGCCAGCGGGAACATATCAACAAAGCCTTGCa                                                                                                          <  1:613555/140‑1 (MQ=255)
                      ttCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCAAGGCCAGCGGGAACATATCAACAAAGCCTTGCa                                                                                                          <  1:611858/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  1:301773/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  2:1173103/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  2:1172957/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  1:1122866/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  2:26705/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  1:301806/140‑1 (MQ=255)
                        cTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAgg                                                                                                        <  1:354739/140‑1 (MQ=255)
                         tttCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCGGCGGGAACATATCAACAAAGCCTTGCAGGa                                                                                                       >  1:542104/1‑140 (MQ=255)
                         tttCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGa                                                                                                       >  1:37303/1‑140 (MQ=255)
                                     ccAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCg                                                                                           >  2:1136919/1‑140 (MQ=255)
                                       aGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGAc                                                                                         <  2:933349/140‑1 (MQ=255)
                                       aGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGAc                                                                                         <  2:237405/140‑1 (MQ=255)
                                       aGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGAc                                                                                         <  2:237781/140‑1 (MQ=255)
                                        ggcggcGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATACAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGAcc                                                                                        >  2:165428/1‑140 (MQ=255)
                                                      gggCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTg                                                                          <  2:800547/140‑1 (MQ=255)
                                                      gggCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTg                                                                          <  2:435528/140‑1 (MQ=255)
                                                      gggCGTTCGCCGGGAATGATGGCGCTTCAGTGTAGCCGAGCATAAAGCGCTTAAAGAACATGGAGATCACTGCACTGTCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTTAATAGCGAAAAGAAAAAGAGGCTg                                                                          <  2:799180/140‑1 (MQ=255)
                                                        gCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTa                                                                        <  2:776829/140‑1 (MQ=255)
                                                        gCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTa                                                                        <  2:776845/140‑1 (MQ=255)
                                                        gCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTa                                                                        <  2:142838/140‑1 (MQ=255)
                                                                              cTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCa                                                  <  1:1136919/140‑1 (MQ=255)
                                                                               ttCCGAGAAGCCGAGCATAAAGCGCATAACGAACATGGAGATCCCTGCCCAGGCCAGCTGTAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACAAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCaa                                                 <  2:735889/140‑1 (MQ=255)
                                                                               ttCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGTGGCTGTAGGTGTAAACTTTTTTCGAGCCaa                                                 <  2:734989/140‑1 (MQ=255)
                                                                               ttCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCaa                                                 <  2:735912/140‑1 (MQ=255)
                                                                               ttCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCaa                                                 <  2:966344/140‑1 (MQ=255)
                                                                                   gagaAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACtt                                             <  1:51922/140‑1 (MQ=255)
                                                                                                   tgcgcATAAAGAACATGGAGATCCCTGCCCAGGCCATCGGGAACATATCAACAAAGCCTTGCAGGAAGGAGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAgccg                             >  1:183466/2‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:737985/1‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:368439/1‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:368282/1‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:211478/1‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:1147664/1‑140 (MQ=255)
                                                                                                        aTAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCggg                        >  1:1134040/1‑140 (MQ=255)
                                                                                                                 aTGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCAt               >  2:1168888/1‑140 (MQ=255)
                                                                                                                 aTGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCAt               >  2:357990/1‑140 (MQ=255)
                                                                                                                 aTGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCAt               >  2:1169034/1‑140 (MQ=255)
                                                                                                                  tGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAATCAGCCAGCCGCCGGGGATTTGCATc              >  2:773756/1‑140 (MQ=255)
                                                                                                                  tGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATc              >  2:333207/1‑140 (MQ=255)
                                                                                                                  tGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATc              >  1:1125992/1‑140 (MQ=255)
                                                                                                                          ccTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTa      >  1:948826/1‑140 (MQ=255)
                                                                                                                           cTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGAAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAg     >  1:718572/1‑140 (MQ=255)
                                                                                                                            tGCCCAGGCCAGCGGGAACATAACAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAATCTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAgg    >  2:593970/1‑140 (MQ=255)
                                                                                                                             gCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAGGc   <  2:337844/140‑1 (MQ=255)
                                                                                                                              cccAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAGGcc  <  1:816912/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                         
AGATGGCGGAGGCAGTACCACGTTCTTTCGTCGGGAACCAGGCGGCGACAATTCGGGCGTTCGCCGGGAATGATGGCGCTTCCGAGAAGCCGAGCATAAAGCGCATAAAGAACATGGAGATCCCTGCCCAGGCCAGCGGGAACATATCAACAAAGCCTTGCAGGAAGGTGAATAGCGACCAGAAAAAGAGGCTGTAGGTGTAAACTTTTTTCGAGCCAAACTTATCAAGCAGCCAGCCGCCGGGGATTTGCATCAGCAAGTAGGCC  >  NC_000913/3274451‑3274716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑