Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,420,607 G→T 5.7% V158V (GTG→GTT yhdX → putative amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,420,6070GT 5.7% 254.2 / 2.6 71V158V (GTG→GTTyhdXputative amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (22/44);  new base T (2/2);  total (24/47)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTTAATAAAA  >  NC_000913/3420470‑3420746
                                                                                                                                         |                                                                                                                                           
agaCTTTCGGATAACTGGCTTCTAAGTAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg                                                                                                                                           <  1:94156/140‑1 (MQ=255)
agaCTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg                                                                                                                                           <  1:998017/140‑1 (MQ=255)
agaCTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg                                                                                                                                           <  1:998072/140‑1 (MQ=255)
agaCTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg                                                                                                                                           <  1:93891/140‑1 (MQ=255)
   cTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAgcgc                                                                                                                                        >  2:1011714/1‑140 (MQ=255)
   cTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATATTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAgcgc                                                                                                                                        >  2:7911/1‑140 (MQ=255)
       cGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGttt                                                                                                                                    >  1:692854/1‑140 (MQ=255)
       cGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGttt                                                                                                                                    >  1:276496/1‑140 (MQ=255)
              cTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg                                                                                                                             >  2:692159/1‑140 (MQ=255)
              cTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg                                                                                                                             >  2:12707/1‑140 (MQ=255)
                        cGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTTAGCGCGTTTGATCTGGCCTTTTTGAg                                                                                                                   >  2:1141110/1‑140 (MQ=255)
                        cGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTTAGCGCGTTTGATCTGGCCTTTTTGAg                                                                                                                   >  2:1141855/1‑140 (MQ=255)
                               tttCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGt                                                                                                            <  1:1228611/140‑1 (MQ=255)
                               tttCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGt                                                                                                            <  2:1082783/140‑1 (MQ=255)
                               tttCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGAAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGt                                                                                                            <  1:1138127/140‑1 (MQ=255)
                                 tCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTgg                                                                                                          <  2:523944/140‑1 (MQ=255)
                                 tCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTgg                                                                                                          <  2:1020270/140‑1 (MQ=255)
                                  ccACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGAAATCGTggg                                                                                                         >  2:1216130/1‑140 (MQ=255)
                                                 agaTCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCa                                                                                          <  2:603011/140‑1 (MQ=255)
                                                  gaTCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCAc                                                                                         >  1:1127957/1‑140 (MQ=255)
                                                  gaTCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCAc                                                                                         >  1:1127771/1‑140 (MQ=255)
                                                         cGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTTAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                                                                  <  2:558335/140‑1 (MQ=255)
                                                         cGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTTAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                                                                  <  2:558597/140‑1 (MQ=255)
                                                         cGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                                                                  <  2:321043/140‑1 (MQ=255)
                                                         cGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCt                                                                                  <  2:321122/140‑1 (MQ=255)
                                                            aaTATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTggg                                                                               >  2:274399/1‑140 (MQ=255)
                                                            aaTATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTggg                                                                               >  2:127968/1‑140 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTCTATTCCGTCACCGCAGCTGGgaga                                                                            <  2:804268/140‑1 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:1034838/140‑1 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:1033291/140‑1 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:479445/140‑1 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:805258/140‑1 (MQ=255)
                                                               atTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGATTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:1166180/140‑1 (MQ=255)
                                                               agtCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATGTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGgaga                                                                            <  2:999329/138‑1 (MQ=255)
                                                                ttCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGAc                                                                           <  1:34437/140‑1 (MQ=255)
                                                                   ccccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg                                                                                                                             >  1:317389/1‑87 (MQ=255)
                                                                   ccccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg                                                                                                                             <  2:317389/87‑1 (MQ=255)
                                                                   ccccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGa                                                                        >  1:822502/1‑140 (MQ=255)
                                                                     ccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGAtt                                                                      >  1:806494/1‑140 (MQ=255)
                                                                     ccGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGAtt                                                                      >  1:475085/1‑140 (MQ=255)
                                                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTAt                                                                   <  1:251874/140‑1 (MQ=255)
                                                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTAt                                                                   <  2:415370/140‑1 (MQ=255)
                                                                        ctgctgCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTAt                                                                   <  2:415265/140‑1 (MQ=255)
                                                                        cagcagcAAATCTTCTTCTGGTACTATCCAGCGAACCGCAATTAGCCCGGACCACGCCAGGCTTTCAGCGCGTTTGCTCTGGCCAATTTGACCAATCTTTCGCTTTATATTCCCTCACCGCACCTTTGAGACTGATTTAt                                                                   <  2:650963/135‑1 (MQ=255)
                                                                                 aTCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTTAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTAt                                                          <  1:1141855/140‑1 (MQ=255)
                                                                                 aTCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTTAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTAt                                                          <  1:1141110/140‑1 (MQ=255)
                                                                                    ttcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCt                                                       <  2:339311/140‑1 (MQ=255)
                                                                                    ttcttctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCt                                                       <  2:320929/140‑1 (MQ=255)
                                                                                         ctGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCtg                                                  <  1:326595/140‑1 (MQ=255)
                                                                                               cTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTa                                            <  1:391094/140‑1 (MQ=255)
                                                                                               cTTTGCCGTGTTGCGCAATTTGCCAGGACCACGACAGGCTGTGAGCGCGTTAGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTa                                            <  1:555631/140‑1 (MQ=255)
                                                                                                tttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTAt                                           <  1:226192/140‑1 (MQ=255)
                                                                                                tttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTAt                                           <  1:226200/140‑1 (MQ=255)
                                                                                                attGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGATTGATCTTGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTAt                                           <  2:1172085/139‑1 (MQ=255)
                                                                                                 ttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATg                                          >  2:348919/1‑140 (MQ=255)
                                                                                                 ttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATg                                          >  1:988821/1‑140 (MQ=255)
                                                                                                 ttGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATACTGGCTGTTGTTATg                                          >  1:489273/1‑140 (MQ=255)
                                                                                                    ccGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCt                                       <  1:298281/140‑1 (MQ=255)
                                                                                                    ccGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCt                                       <  1:1107355/140‑1 (MQ=255)
                                                                                                     cGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCta                                      <  2:808027/140‑1 (MQ=255)
                                                                                                            cgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCt                               <  1:1114672/140‑1 (MQ=255)
                                                                                                            cgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCt                               <  1:884055/140‑1 (MQ=255)
                                                                                                            cgcAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCt                               <  1:530930/140‑1 (MQ=255)
                                                                                                                      ccGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTggg                     <  2:530152/140‑1 (MQ=255)
                                                                                                                      ccGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTggg                     <  1:315646/140‑1 (MQ=255)
                                                                                                                              cGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGTTTTTTTGCTTTTAGCCTGGCTGTTGTTATGTCTATAGTCCTTTCTGTAGGGCTATTcct             <  2:684977/140‑2 (MQ=255)
                                                                                                                              cGCCAGGCTGTGAGCGCGTTAGATCAGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCAGGGAGACGGATTTATTGCGATTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCg             <  2:602086/140‑1 (MQ=255)
                                                                                                                                    gCTGTGAGCGCGTTTGATCTGGCCTTTTTGTGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTGCTTTCTGTTGGGCTATTCCGCtttta       >  2:245757/1‑138 (MQ=255)
                                                                                                                                    gCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTtaa       >  1:1167749/1‑140 (MQ=255)
                                                                                                                                    gCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTtaa       >  2:996324/1‑140 (MQ=255)
                                                                                                                                    gCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTtaa       >  1:1085374/1‑140 (MQ=255)
                                                                                                                                      tgtgAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTAATTCGTTTATAATTGCTGTTGTTAAGGCAAAAGTACATTATTGTGGGCTAATCCGCTTtaataa    >  1:624903/1‑140 (MQ=255)
                                                                                                                                         gAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTTAATaaaa  >  1:563940/1‑140 (MQ=255)
                                                                                                                                         gAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTTAATaaaa  >  2:913027/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGGGAGACGGATTTATTGCGTTTATCCTGGCTGTTGTTATGGCTATAGTCCTTTCTGTTGGGCTATTCCGCTTTAATAAAA  >  NC_000913/3420470‑3420746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: