Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,861 T→A 11.8% intergenic (+78/+74) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8610TA11.8% 466.3 / 4.0 215intergenic (+78/+74)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (25/139);  new base A (31/2);  total (74/141)
Fisher's exact test for biased strand distribution p-value = 1.59e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

TCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGT  >  NC_000913/3504722‑3504999
                                                                                                                                           |                                                                                                                                          
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:49921/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  2:563362/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:447063/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:213645/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCt                                                                                                                                            <  2:36118/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATAGATAATAAAAGACATAAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:1068126/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATAGATAATAAAAGACATAAACCGTAGGACGGATAAGATGAGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  2:7982/140‑1 (MQ=255)
tcaccGAACACTTCTGCCGCGCCAATCGCATCACCTTAACTATTCATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGAGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:1213813/137‑1 (MQ=255)
    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAcc                                                                                                                                        >  1:113297/1‑140 (MQ=255)
    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAcc                                                                                                                                        >  1:419764/1‑140 (MQ=255)
       aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                     <  1:852079/140‑1 (MQ=255)
         tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCaaaa                                                                                                                                   >  1:865624/1‑139 (MQ=255)
         tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAc                                                                                                                                   >  2:819112/1‑140 (MQ=255)
          cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                  <  1:236108/140‑1 (MQ=255)
          cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                  <  1:35015/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  2:1098915/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  1:632462/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  1:1099759/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  1:870736/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGACGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTTACCct                                                                                                                                <  2:170281/140‑1 (MQ=255)
            tctGCCGCGCAAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  2:51119/140‑1 (MQ=255)
              tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                              <  1:885237/140‑1 (MQ=255)
                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAaccacacc                                                                                                                            >  1:601709/1‑135 (MQ=39)
                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACAGTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCTCACCCCa                                                                                                                         >  2:847298/1‑140 (MQ=255)
                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCACCAAACCCGCACCCCaaa                                                                                                                       >  1:469644/1‑140 (MQ=38)
                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                       <  2:800971/140‑1 (MQ=255)
                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                       <  2:589473/140‑1 (MQ=255)
                       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                     <  2:1060340/140‑1 (MQ=255)
                       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                     <  1:779679/140‑1 (MQ=255)
                       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                     <  1:30993/140‑1 (MQ=255)
                            cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                <  1:915694/140‑1 (MQ=255)
                            cATCACCTTAACTATTGAGAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTACCCCTCTCCCCAAAGGGGCGa                                                                                                                <  2:1050576/140‑1 (MQ=255)
                                 ccatAACTATTGAAAATAAAAGACACTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                           <  2:404904/137‑1 (MQ=255)
                                 ccTTAACTATTGATAATAATAGACATTAACCGTTGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                           <  2:1068758/140‑1 (MQ=255)
                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                           <  1:972392/140‑1 (MQ=255)
                                 acTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                           <  2:15750/139‑1 (MQ=255)
                                  cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                          >  2:1076437/1‑140 (MQ=255)
                                    tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACAACCCAAAGGGGCGAGGGGAccc                                                                                                        >  2:967798/1‑139 (MQ=255)
                                     aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGt                                                                                                       >  2:1195755/1‑140 (MQ=255)
                                      acaaTTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCGTCACGCTAACCCTCTCCCCACAGGGGCGAGGGGACCGta                                                                                                      <  2:1072302/137‑2 (MQ=38)
                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                      <  1:76874/140‑1 (MQ=255)
                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                      <  2:1006652/140‑1 (MQ=255)
                                       cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                     <  1:715909/140‑1 (MQ=255)
                                           tGATAATAATAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTa                                                                                                 >  2:300869/1‑140 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                 <  1:834195/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                 <  1:401219/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                 <  1:817250/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                 <  1:773831/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                 <  1:726506/140‑1 (MQ=255)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCATCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCGTCACCCTAACCCAGTCCCCAAATGGGCGAGGTGACCGTTcgcgac                                                                                                >  1:1207060/1‑135 (MQ=38)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCAAAACCACAACCCCAAGGGGCGGGGGGTCCGGTAAGTAc                                                                                                >  1:364020/1‑140 (MQ=37)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                >  2:1200694/1‑140 (MQ=255)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCCTTCAGTAc                                                                                                >  1:590454/1‑140 (MQ=255)
                                            gATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCACCCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                >  2:881549/1‑140 (MQ=39)
                                                ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCTTCGCATCAGGCGATGCTGGCGCGATGATTTTTACATGCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                            <  1:570406/140‑1 (MQ=38)
                                                 taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAAAGGGGGGGGGGTCCCTTCAGTAAAGAAt                                                                                           >  1:91193/1‑140 (MQ=25)
                                                    aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCGCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAATTTa                                                                                        >  2:932830/1‑140 (MQ=255)
                                                    aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCAGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCACTACGGAAATTa                                                                                        >  1:1084970/1‑140 (MQ=255)
                                                     aGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                       <  2:21671/140‑1 (MQ=255)
                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACTCCCCAAAAGGGCGAGGGGGCCGTTCAGTACGGGAATTATCTTg                                                                                   >  1:41879/1‑140 (MQ=37)
                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACAACCAAACCCACAACCCAAATGGGCGGGGGGGCCCGTCAGTAAGGAAATTAGCGTg                                                                                   >  1:803830/1‑140 (MQ=25)
                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTg                                                                                   >  2:777309/1‑140 (MQ=255)
                                                          ttAACCGTAGGCCGTATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  2:455441/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  1:1006993/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  2:415614/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  1:553056/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  1:522326/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  1:701032/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                  <  1:388882/140‑1 (MQ=255)
                                                                   ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACAACCCAAACGGGCGCGGGGACCGGTAAGTTACGAAATTAGCATTAAGGTGCTAt                                                                         >  1:460267/1‑140 (MQ=19)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACTTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                        <  2:637693/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                        <  1:1195755/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                        <  2:927482/140‑1 (MQ=255)
                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAAACCACACCCCAAAGGGGCGGGGGGACCGTTCAGTACGGAAATTAGCATGACGGTGCTAACCCGc                                                                    >  2:1016702/1‑140 (MQ=255)
                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAACCGAACCCGAAACCAAGCCACCAAGTGGGGGTGGGACCGGTCATTACCGAATTTATGGTGAACGTGCTATACAgg                                                                    >  1:18100/1‑139 (MQ=255)
                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAAGGGCGAGGGGGCCCATCAATAAGGAAATTATCATTAAGGTGCTaaacacca                                                                   >  1:904803/1‑133 (MQ=255)
                                                                           aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                 <  2:287311/140‑1 (MQ=255)
                                                                           aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                 <  1:491826/140‑1 (MQ=255)
                                                                             gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGACCACAACCGATCCCACTGCCCCCAGGGGGGAGGGGTACGGTCAGTACGGGATTTTGCTTGTCGGTGCGATAAAGCATTat                                                               >  1:195360/1‑139 (MQ=255)
                                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGAGCTATCCAGCATTAAAt                                                             <  2:562478/140‑1 (MQ=255)
                                                                                 cgcCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTCCCCCATGGGGCGGGGGGACCGGTCAGTACGGAATTTAGCGGGACGGTGCTATCAAGCAtgataaac                                                           >  2:1183071/1‑133 (MQ=255)
                                                                                 cgcCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACAACCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                           <  1:1031682/140‑1 (MQ=255)
                                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                          <  2:578848/140‑1 (MQ=255)
                                                                                  accAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTATCTTGAAGGTTCTATACACCAATAAATACt                                                          >  2:60900/2‑140 (MQ=255)
                                                                                       catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                     <  1:157623/140‑1 (MQ=255)
                                                                                         tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                   <  1:579849/140‑1 (MQ=255)
                                                                                            catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCACAACCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCATGACGGTGCTATACAGCATTAAATACTTGCAATAcac                                                >  2:840807/1‑138 (MQ=255)
                                                                                                     aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCGCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCACCATTAAATACTGGCAATACTCTCACGCaaa                                       <  1:649050/140‑1 (MQ=255)
                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGATGGGTCCGTTCAGTACGGAATTTAGCTTGACGGGGCTATACAGCAGTTAATACTGGCAATACTCTCACGCAAAAACCgc                                 >  2:464357/1‑140 (MQ=255)
                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTACCCAAAGGGGCGAGGGGACCGGTGAGTACGGTAATTAGCTTGAAGGTTCTATCCAGCATTAAATAATGGCAATACTCAAAAGCAAAATACgc                                 >  2:69031/1‑140 (MQ=255)
                                                                                                              cgTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAATTACTGCCAATACTCTCACGCAAAATCCgcagc                              <  2:991988/140‑1 (MQ=255)
                                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTTAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                            <  1:455976/140‑1 (MQ=255)
                                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                            <  1:25777/140‑1 (MQ=255)
                                                                                                                atGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                            <  2:1137183/139‑1 (MQ=255)
                                                                                                                           aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGAAAAATCCGCAGCACCGTCTCTTCAc                 <  2:351354/140‑1 (MQ=255)
                                                                                                                             cccGTACGTTACCCACACCCAAAACCACAACCCAAAGGGGCGAGGGGGCCCGTCAGTAAGGAATTTATCTTGACGGTGCTATCCAGCATTAAAAAAAGGCAAAACACACACGCAAAATACGCAGCACCGTCTCGTCaacc               >  2:18624/1‑137 (MQ=255)
                                                                                                                                gTACGGTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg            <  2:434475/140‑1 (MQ=255)
                                                                                                                                 tACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAATTTATCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACGCAAAAACCCCAGCACCGTCTCTTCACCGCTGc           >  2:376994/1‑140 (MQ=38)
                                                                                                                                 tACGTTACCCGCACCCTAACCCTCTCCCCAAAGGGGCCAGGGGACCGTTCAGTACGGAATTAAGCTTGAAGGTGCTATCCAGCAGAAAATAAGGGCAATACACACAAGCAAAAAACCCCGCACCGGCTATACAACGCAGc           >  2:1149029/1‑140 (MQ=255)
                                                                                                                                          cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt  <  2:241367/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGT  >  NC_000913/3504722‑3504999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: