Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,867 T→A 16.4% intergenic (+84/+68) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8670TA16.4% 408.8 / 18.4 212intergenic (+84/+68)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (27/138);  new base A (41/3);  total (71/141)
Fisher's exact test for biased strand distribution p-value = 9.03e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NC_000913/3504729‑3505006
                                                                                                                                           |                                                                                                                                            
aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                             <  1:852079/140‑1 (MQ=255)
  tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCaaaa                                                                                                                                           >  1:865624/1‑139 (MQ=255)
  tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAc                                                                                                                                           >  2:819112/1‑140 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  1:444337/140‑1 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  1:236108/140‑1 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  2:176116/140‑1 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAccc                                                                                                                                         >  2:942459/1‑140 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAccc                                                                                                                                         >  2:610323/1‑140 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACAAGAGATAATAACAGACATTAACCGTAGGCCGGATAATATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGGGAATGTTACATCCCGGACGATACCCACACCCAAAacc                                                                                                                                         >  2:603752/1‑140 (MQ=25)
     tctGCCGCGCCATTCGCTTCAGCTTTTCTTTTGATTATTATAGTCATTATCCGTAGGCCGGTGAAGTGGCGCCTGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGGACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:1231454/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATGACCTTATCTATTGTTAATATAAGACATTAACCGTAGGCCGGATAAGTTGCGCCTGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:1086117/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:157349/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:159986/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:870736/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:1099759/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:632425/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:567862/140‑1 (MQ=255)
     tctGCCGCGCAAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:51119/140‑1 (MQ=255)
     tctGCCGCGACAATCGCATCACCTTAACTATTGATAATAAATGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:41706/140‑1 (MQ=255)
      ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAcccgc                                                                                                                                       >  1:756026/1‑138 (MQ=255)
         ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAaccacacc                                                                                                                                    >  1:601709/1‑135 (MQ=39)
            cgcCAATCGCATCACCTTTACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                <  1:504236/140‑1 (MQ=255)
            cgcCAATCGCATCACCTTCACTATTGATACTAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                <  1:459221/140‑1 (MQ=255)
            cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                >  2:1191420/1‑140 (MQ=255)
              ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTATGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                              <  2:800951/140‑1 (MQ=255)
              ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACACCCCaaa                                                                                                                              >  1:469606/1‑140 (MQ=255)
                caTCGCATCACCATACCTATTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                            <  2:916428/139‑1 (MQ=255)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                            <  2:1060340/140‑1 (MQ=255)
                   cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                         <  1:414122/140‑1 (MQ=255)
                     cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                       <  1:779312/140‑1 (MQ=255)
                          ccatAACTATTGAAAATAAAAGACACTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                  <  2:404904/137‑1 (MQ=255)
                          ccTTAACTATTGATAATAATAGACATTAACCGTTGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                  <  2:1068758/140‑1 (MQ=255)
                          ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                  <  2:764242/140‑1 (MQ=255)
                          ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                  <  1:874731/140‑1 (MQ=255)
                          acTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                  <  2:15750/139‑1 (MQ=255)
                           cTTAACTATTGATAATAAATGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTATACCTCTCCCCAAAGGGGCGAGGGGaa                                                                                                                 >  2:1074794/1‑139 (MQ=39)
                             tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCACCCCAAAGGGGCGAGGGGACCg                                                                                                               >  2:582546/1‑140 (MQ=255)
                             tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACAACCCAAAGGGGCGAGGGGAccc                                                                                                               >  2:967798/1‑139 (MQ=255)
                               acaaTTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCGTCACGCTAACCCTCTCCCCACAGGGGCGAGGGGACCGta                                                                                                             <  2:1072302/137‑2 (MQ=38)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                             <  1:1211233/140‑1 (MQ=255)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                             <  2:1006652/140‑1 (MQ=255)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                             <  1:322623/140‑1 (MQ=255)
                                cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                            <  1:645197/140‑1 (MQ=255)
                                cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                            <  1:715909/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                        <  1:817250/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                        <  1:583584/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                        <  1:401219/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                        <  1:773753/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCAAAGGGGCGAGGGGACCCTTCATTa                                                                                                        >  2:300683/1‑140 (MQ=38)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACAACCCCAACGGGCGGGGGGACCGGTAAGTa                                                                                                        >  1:987619/1‑140 (MQ=37)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCAAAACCACAACCCCAAGGGGCGGGGGGTCCGGTAAGTAc                                                                                                       >  1:364020/1‑140 (MQ=37)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCAAACCCAAAACCCAAAACCAAAAGGGGGGGGGGGGCCGtaagtaa                                                                                                        >  1:680987/1‑134 (MQ=21)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCGCACCCCAAAGGGGGGAGGGGGCCGGTCAGTAc                                                                                                       >  1:452042/1‑140 (MQ=38)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCAGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCACAAACCAAACCCACAACACAAAGGGGGGTGGGGAACAGTCAGTaa                                                                                                       >  1:249321/1‑139 (MQ=25)
                                      ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                      <  2:375133/140‑1 (MQ=255)
                                        aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGAACGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGATGGGACCGTTCATGACGGa                                                                                                    >  2:201984/1‑140 (MQ=38)
                                          taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAAAGGGGGGGGGGTCCCTTCAGTAAAGAAt                                                                                                  >  1:91193/1‑140 (MQ=25)
                                             aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCCGTAAGTACGGAAATTa                                                                                               >  2:929340/1‑140 (MQ=38)
                                             aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCAGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCACTACGGAAATTa                                                                                               >  1:1084970/1‑140 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACAACCAAACCCACAACCCAAATGGGCGGGGGGGCCCGTCAGTAAGGAAATTAGCGTg                                                                                          >  1:803830/1‑140 (MQ=25)
                                                   ttAACCGTAGGCCGTATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  2:455441/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCTTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  2:1056625/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  1:1006993/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  1:522326/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  1:1078286/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  1:701051/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  1:835607/140‑1 (MQ=255)
                                                   attaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGACTTTTACATCCCGTACGTTCCCCTCACCCTAACCCCCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  2:223762/137‑1 (MQ=39)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  2:119598/140‑1 (MQ=255)
                                                          tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCTCTACCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGAACGTGCt                                                                                  >  2:86706/1‑140 (MQ=38)
                                                            ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCCAAGGGGCCGGGGGGCCCGTCCCGAACGAATTTAAGCATGACgtgcgat                                                                                 >  1:206722/1‑134 (MQ=19)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACTTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  2:637693/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:792448/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:892285/140‑1 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAAACCACACCCCAAAGGGGCGGGGGGACCGTTCAGTACGGAAATTAGCATGACGGTGCTAACCCGc                                                                           >  2:1016702/1‑140 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAACCGAACCCGAAACCAAGCCACCAAGTGGGGGTGGGACCGGTCATTACCGAATTTATGGTGAACGTGCTATACAgg                                                                           >  1:18100/1‑139 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                          >  2:81179/1‑140 (MQ=255)
                                                                    aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                        <  1:491826/140‑1 (MQ=255)
                                                                      gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGACCACAACCGATCCCACTGCCCCCAGGGGGGAGGGGTACGGTCAGTACGGGATTTTGCTTGTCGGTGCGATAAAGCATTat                                                                      >  1:195360/1‑139 (MQ=255)
                                                                        tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGAGCTATCCAGCATTAAAt                                                                    <  2:562478/140‑1 (MQ=255)
                                                                        tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                    <  1:35524/140‑1 (MQ=255)
                                                                        tGCGCCAGCATCGCATCCGGCGAAGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCCCTCCCCAAACGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                    <  1:579108/140‑1 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTCCCCCATGGGGCGGGGGGACCGGTCAGTACGGAATTTAGCGGGACGGTGCTATCAAGCAtgataaac                                                                  >  2:1183071/1‑133 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACAACCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                  <  1:1031682/140‑1 (MQ=255)
                                                                                     catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCTAACCCGCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGTATTTAGCTTGACGGTGCTATCCAGCGTTAAATACTGGCAATAcac                                                       >  2:841676/1‑138 (MQ=38)
                                                                                                gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCACCCCCAAGGGGCGAGGGGACCGTTAAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACGCAAAAt                                            >  2:498756/1‑140 (MQ=38)
                                                                                                    gcgcGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTACCCAAAGGGGCGAGGGGACCGGTGAGTACGGTAATTAGCTTGAAGGTTCTATCCAGCATTAAATAATGGCAATACTCAAAAGCAAAATACgc                                        >  2:69031/1‑140 (MQ=255)
                                                                                                     cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                       <  1:953165/140‑1 (MQ=255)
                                                                                                       cgTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAATTACTGCCAATACTCTCACGCAAAATCCgcagc                                     <  2:991988/140‑1 (MQ=255)
                                                                                                         atGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                   <  2:1137183/139‑1 (MQ=255)
                                                                                                          tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGcccc                                  <  1:1140025/140‑1 (MQ=255)
                                                                                                          tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                  <  1:309877/140‑1 (MQ=255)
                                                                                                          tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                  <  1:1200694/140‑1 (MQ=255)
                                                                                                                    aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGAAAAATCCGCAGCACCGTCTCTTCAc                        <  2:351354/140‑1 (MQ=255)
                                                                                                                         gTACGTTCCCCTCACCCTAACCCTCTCCCCTAAGCGGCGAGGGGACCGTTGAGTACGGAATTTAGATTGACGGTGCTATCCAGCATTAAATTCTGGCAATACTCTCACGCTAAATCCGCAGCACCGTCTCTTCACCGCTg                   <  2:331658/140‑1 (MQ=39)
                                                                                                                         gTACGGTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                   <  2:434475/140‑1 (MQ=255)
                                                                                                                          tACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAATTTATCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACGCAAAAACCCCAGCACCGTCTCTTCACCGCTGc                  >  2:376994/1‑140 (MQ=38)
                                                                                                                          tACGTTACCCGCACCCTAACCCTCTCCCCAAAGGGGCCAGGGGACCGTTCAGTACGGAATTAAGCTTGAAGGTGCTATCCAGCAGAAAATAAGGGCAATACACACAAGCAAAAAACCCCGCACCGGCTATACAACGCAGc                  >  2:1149029/1‑140 (MQ=255)
                                                                                                                                  ccacACCCAAAACCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACCGAAATTAGCATGACGGTGCTAACCAGCATTAAATACAGGCAATAATCTCAAGCAAAATCCGCAGCACCCTAACTTCACCGCAGCCATTCggg          >  2:832888/4‑140 (MQ=255)
                                                                                                                                   cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt         <  2:241367/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NC_000913/3504729‑3505006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: