Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,875 T→A 15.5% intergenic (+92/+60) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8750TA15.5% 375.7 / 15.1 186intergenic (+92/+60)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (33/114);  new base A (34/3);  total (68/118)
Fisher's exact test for biased strand distribution p-value = 4.05e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

TGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NC_000913/3504736‑3505014
                                                                                                                                           |                                                                                                                                           
     cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                        <  1:809340/140‑1 (MQ=255)
     cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                        <  1:233826/140‑1 (MQ=255)
       ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCACCAAACCCGCACCCCaaa                                                                                                                                      >  1:469644/1‑140 (MQ=38)
       ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                      <  2:800971/140‑1 (MQ=255)
       ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                      <  2:589473/140‑1 (MQ=255)
       ccAATCCCATCACCTTAACAATTGATAAAAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGAAGCTCGCGCGGTGAATGTTACATCCCGTACGTACCCCACAACCAAACCCACACCCCaaa                                                                                                                                      >  2:824390/1‑140 (MQ=25)
         caTCGCATCACCATACCTATTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                    <  2:916428/139‑1 (MQ=255)
         aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                    <  2:1060340/140‑1 (MQ=255)
         aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                    <  1:779679/140‑1 (MQ=255)
         aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                    <  1:30993/140‑1 (MQ=255)
                   ccTTACCTATTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                          <  2:504205/140‑1 (MQ=255)
                   ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                          <  1:972392/140‑1 (MQ=255)
                   ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                          <  1:715049/140‑1 (MQ=255)
                    cTTAACTATTGATAATAAATGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTATACCTCTCCCCAAAGGGGCGAGGGGaa                                                                                                                         >  2:1074794/1‑139 (MQ=39)
                      tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCACCCCAAAGGGGCGAGGGGACCg                                                                                                                       >  2:582546/1‑140 (MQ=255)
                      tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACAACCCAAAGGGGCGAGGGGAccc                                                                                                                       >  2:967798/1‑139 (MQ=255)
                       aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGt                                                                                                                      >  2:1195755/1‑140 (MQ=255)
                        acaaTTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCGTCACGCTAACCCTCTCCCCACAGGGGCGAGGGGACCGta                                                                                                                     <  2:1072302/137‑2 (MQ=38)
                        aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                     <  1:322623/140‑1 (MQ=255)
                         cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                                    <  1:40380/140‑1 (MQ=255)
                         cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                                    <  1:645197/140‑1 (MQ=255)
                             tGATAATAATAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTa                                                                                                                >  2:300869/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                <  1:834195/140‑1 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                <  1:773831/140‑1 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                <  1:726506/140‑1 (MQ=255)
                              gATAATAAACGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTAc                                                                                                               <  1:1167010/140‑1 (MQ=255)
                              gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCAAAACCACAACCCCAAGGGGCGGGGGGTCCGGTAAGTAc                                                                                                               >  1:364020/1‑140 (MQ=37)
                              gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                               >  2:1200694/1‑140 (MQ=255)
                              gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCGCACCCCAAAGGGGGGAGGGGGCCGGTCAGTAc                                                                                                               >  1:452042/1‑140 (MQ=38)
                              gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCCTTCAGTAc                                                                                                               >  1:590454/1‑140 (MQ=255)
                              gATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCACCCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                               >  2:881549/1‑140 (MQ=39)
                               ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCGGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                              <  2:375299/140‑1 (MQ=255)
                                 aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGAACGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGATGGGACCGTTCATGACGGa                                                                                                            >  2:201984/1‑140 (MQ=38)
                                  ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCTTCGCATCAGGCGATGCTGGCGCGATGATTTTTACATGCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                           <  1:570406/140‑1 (MQ=38)
                                      aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCCACCCCCAACGGGCGAGGGGACCGGTCACTAAGGAATTTa                                                                                                       >  1:906363/1‑140 (MQ=37)
                                           aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTg                                                                                                  >  2:777309/1‑140 (MQ=255)
                                            ttAACCGTAGGCCGTATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:455441/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCTTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:1056625/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:553056/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:1011853/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:701032/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:1006993/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:869593/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:388882/140‑1 (MQ=255)
                                             tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAGGGGCGAGGGGGCCCGTCAGTACCGAAATTAGCATGAc                                                                                                >  1:1117141/1‑140 (MQ=37)
                                                   tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCTCTACCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGAACGTGCt                                                                                          >  2:86706/1‑140 (MQ=38)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACAACCCAAACGGGCGCGGGGACCGGTAAGTTACGAAATTAGCATTAAGGTGCTAt                                                                                        >  1:460267/1‑140 (MQ=19)
                                                      gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACTTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                       <  2:637693/140‑1 (MQ=255)
                                                      gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                       <  2:927482/140‑1 (MQ=255)
                                                             aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                                <  1:491826/140‑1 (MQ=255)
                                                               gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGACCACAACCGATCCCACTGCCCCCAGGGGGGAGGGGTACGGTCAGTACGGGATTTTGCTTGTCGGTGCGATAAAGCATTat                                                                              >  1:195360/1‑139 (MQ=255)
                                                                 tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGAGCTATCCAGCATTAAAt                                                                            <  2:562478/140‑1 (MQ=255)
                                                                 tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                            <  1:35524/140‑1 (MQ=255)
                                                                   cgcCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTCCCCCATGGGGCGGGGGGACCGGTCAGTACGGAATTTAGCGGGACGGTGCTATCAAGCAtgataaac                                                                          >  2:1183071/1‑133 (MQ=255)
                                                                         catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                    <  1:157623/140‑1 (MQ=255)
                                                                           tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                  <  1:579849/140‑1 (MQ=255)
                                                                              catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCTAACCCGCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGTATTTAGCTTGACGGTGCTATCCAGCGTTAAATACTGGCAATAcac                                                               >  2:841676/1‑138 (MQ=38)
                                                                                tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAAGTTAGCTTGACGGTGCTATCCCGCAGGAAATACGGGCCATACtctc                                                             >  2:409950/1‑140 (MQ=38)
                                                                                             gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGATGGGTCCGTTCAGTACGGAATTTAGCTTGACGGGGCTATACAGCAGTTAATACTGGCAATACTCTCACGCAAAAACCgc                                                >  2:464357/1‑140 (MQ=255)
                                                                                             gcgcGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTACCCAAAGGGGCGAGGGGACCGGTGAGTACGGTAATTAGCTTGAAGGTTCTATCCAGCATTAAATAATGGCAATACTCAAAAGCAAAATACgc                                                >  2:69031/1‑140 (MQ=255)
                                                                                                  ttGAATTTTACATCCCGTACGTTCCCCTCTCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                           <  2:114444/140‑1 (MQ=255)
                                                                                                  ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                           <  1:25777/140‑1 (MQ=255)
                                                                                                  ttGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACAACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGAAGGTGCTATACAGCAATAAAAACAGGCAATACAAGCACGCAAAATACCCAGCAc                                           >  2:432289/1‑140 (MQ=25)
                                                                                                  atGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                           <  2:1137183/139‑1 (MQ=255)
                                                                                                   tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                          <  1:1200694/140‑1 (MQ=255)
                                                                                                             aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGAAAAATCCGCAGCACCGTCTCTTCAc                                <  2:351354/140‑1 (MQ=255)
                                                                                                                            cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt                 <  2:241367/140‑1 (MQ=255)
                                                                                                                                   tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCg          <  2:157027/140‑1 (MQ=255)
                                                                                                                                   tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCg          <  1:223563/140‑1 (MQ=255)
                                                                                                                                    aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTCTCCAGCATTAAATACTGGCAATACTCTCACGCAATATCCGCAGCACCGTCTCGTCACCGCTGCGATTCGGGTTAATGCGa         >  1:668405/1‑140 (MQ=255)
                                                                                                                                    aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  1:1053111/1‑140 (MQ=255)
                                                                                                                                    aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  2:288517/1‑140 (MQ=255)
                                                                                                                                       ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc      <  2:1037941/140‑1 (MQ=255)
                                                                                                                                       ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc      <  1:521756/140‑1 (MQ=255)
                                                                                                                                         tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  2:137966/1‑140 (MQ=255)
                                                                                                                                         tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  1:886368/1‑140 (MQ=255)
                                                                                                                                         tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  1:1082053/1‑140 (MQ=255)
                                                                                                                                          ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  1:1077439/140‑1 (MQ=255)
                                                                                                                                          ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   >  1:435937/1‑140 (MQ=255)
                                                                                                                                          ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  1:881549/140‑1 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCTCCGTCTCTTCACCGCTGCGAGTCGGGTTAATGCGAATCGcac  >  2:1163718/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTATTGCGAATCGcac  >  1:950161/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:900638/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:753114/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:924009/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:338980/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:256620/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:17703/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:857141/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:744728/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:539023/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:479953/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:274098/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:184434/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1209998/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1088252/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1020156/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAGTCGGGTTAATGCGAATCGcac  >  2:39017/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATACGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:180989/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGATTGACGGGGCTATCCAGCATTAAATACTGGCATTACTCTCACGCAAAATCCGCTGCACCGTCTCTTCTCCGCTGCGAGTCGGGTTAATGCGAATCGctc  >  2:961776/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NC_000913/3504736‑3505014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: