Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,533,137 G→T 7.7% G107V (GGC→GTC)  pck → phosphoenolpyruvate carboxykinase [ATP]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,533,1370GT 7.7% 167.9 / 3.3 52G107V (GGC→GTC) pckphosphoenolpyruvate carboxykinase [ATP]
Reads supporting (aligned to +/- strand):  ref base G (31/16);  new base T (2/2);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATT  >  NC_000913/3533007‑3533276
                                                                                                                                  |                                                                                                                                           
ggTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTACGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGAc                                                                                                                                    >  1:404857/1‑140 (MQ=255)
ggTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGTCCTGGTGAc                                                                                                                                    >  1:1085614/1‑140 (MQ=255)
ggTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGTCCTGGTGAc                                                                                                                                    >  1:1085628/1‑140 (MQ=255)
 gTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGAcc                                                                                                                                   >  2:186932/1‑140 (MQ=255)
     ttCACCAAAAGATAAGTATAACGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGCCCTGGTGACCAGGc                                                                                                                               <  2:372106/140‑1 (MQ=255)
                 tAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCa                                                                                                                   <  2:864673/140‑1 (MQ=255)
                 tAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCa                                                                                                                   <  2:3225/140‑1 (MQ=255)
                  aaGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCaa                                                                                                                  <  2:466914/140‑1 (MQ=255)
                      atatCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGt                                                                                                              <  1:990846/140‑1 (MQ=255)
                        atCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCt                                                                                                            >  1:254953/1‑140 (MQ=255)
                          cGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGt                                                                                                          >  1:153863/1‑140 (MQ=255)
                            tCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAACCCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTc                                                                                                        >  2:945357/1‑140 (MQ=255)
                            tCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTc                                                                                                        >  2:347710/1‑140 (MQ=255)
                              cGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGt                                                                                                      >  1:45412/1‑140 (MQ=255)
                              cGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGt                                                                                                      >  1:45652/1‑140 (MQ=255)
                               gTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGtt                                                                                                     >  1:606826/1‑140 (MQ=255)
                                   cGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAATGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCg                                                                                                 <  2:1166493/140‑1 (MQ=255)
                                       aCCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGGCGACGc                                                                                             >  2:96642/1‑140 (MQ=255)
                                       aCCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGGTGTCGACGc                                                                                             >  2:544956/1‑140 (MQ=255)
                                           cTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCTTCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTc                                                                                         >  1:1013528/1‑140 (MQ=255)
                                           cTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTc                                                                                         >  1:201185/1‑140 (MQ=255)
                                            tCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACGTCTCTCACCGTAAACCTGGCAGCATCTGAAAGGCCTGGTTACCAGGCAGCTTTCCGCCAAACGTATGTACGTTGTGGACGCtgact                                                                                        >  1:293789/1‑136 (MQ=255)
                                             cgcgATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCtg                                                                                       <  1:124768/140‑1 (MQ=255)
                                                  tACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTgtggtg                                                                                  >  1:356028/1‑140 (MQ=255)
                                                    cTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCg                                                                                <  1:65630/140‑1 (MQ=255)
                                                              ggCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATa                                                                      >  2:1039313/1‑140 (MQ=255)
                                                              ggCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCATACCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATa                                                                      >  2:1024237/1‑140 (MQ=255)
                                                                        ggcaaaggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTtc                                                            >  2:664615/1‑140 (MQ=255)
                                                                             aggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTtccgtcc                                                       >  2:1202342/1‑140 (MQ=255)
                                                                             aggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTtccgtcc                                                       >  2:305358/1‑140 (MQ=255)
                                                                               gTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTccgtccgt                                                     >  2:746416/1‑140 (MQ=255)
                                                                                   gAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTtca                                                 >  2:104754/1‑140 (MQ=255)
                                                                                   gAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTtca                                                 >  2:104994/1‑140 (MQ=255)
                                                                                   gAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTtca                                                 >  2:166571/1‑140 (MQ=255)
                                                                                    aaCGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGTCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTtcat                                                <  2:1085614/140‑1 (MQ=255)
                                                                                    aaCGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGTCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTtcat                                                <  2:1085628/140‑1 (MQ=255)
                                                                                         caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCg                                           >  2:1027829/1‑140 (MQ=255)
                                                                                         caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCg                                           >  2:1027979/1‑140 (MQ=255)
                                                                                          aacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGa                                          <  2:22701/140‑1 (MQ=255)
                                                                                          aacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGa                                          <  2:575068/140‑1 (MQ=255)
                                                                                          aacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGa                                          <  2:1180930/140‑1 (MQ=255)
                                                                                          aacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGa                                          <  2:22976/140‑1 (MQ=255)
                                                                                               aCCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTACATCACCGAAGTgg                                     >  2:834171/1‑140 (MQ=255)
                                                                                                ccTCTCTCTCCGGAAACCTGTCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGc                                    <  1:713749/140‑1 (MQ=255)
                                                                                                   ctctctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTg                                 >  1:211640/1‑140 (MQ=255)
                                                                                                    tctctcCGGAAACCAGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTgg                                >  2:985634/1‑140 (MQ=255)
                                                                                                      tctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCa                              >  1:1208808/1‑140 (MQ=255)
                                                                                                      tctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCa                              <  2:957617/140‑1 (MQ=255)
                                                                                                      tctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCa                              <  2:957648/140‑1 (MQ=255)
                                                                                                           ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGgcgc                         >  1:1094655/1‑140 (MQ=255)
                                                                                                                   ggCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTc                 <  2:154471/140‑1 (MQ=255)
                                                                                                                   ggCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTc                 <  2:176851/140‑1 (MQ=255)
                                                                                                                                  gCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTAtt  <  1:885719/140‑1 (MQ=255)
                                                                                                                                  gCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTGTGTCAAAAACATGTTTAtt  >  1:604859/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                           
GGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATT  >  NC_000913/3533007‑3533276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑