Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,593,141 C→A 5.3% E99D (GAG→GAT livF ← branched‑chain amino acid ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,593,1410CA 5.3% 260.0 / 2.5 75E99D (GAG→GATlivFbranched‑chain amino acid ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (49/22);  new base A (2/2);  total (51/24)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GCTGTTCACCGCCGGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGC  >  NC_000913/3593008‑3593280
                                                                                                                                     |                                                                                                                                           
gctgTTCACCGCCGGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTcc                                                                                                                                       >  2:587258/1‑140 (MQ=255)
         cgccgGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATc                                                                                                                              >  2:518120/1‑140 (MQ=255)
                cATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCgaga                                                                                                                       <  2:979293/140‑1 (MQ=255)
                     tGCCCGCCCGCTGAATACGGCGCTCAAGCAGACGTCGAAACAGCTCATACAACCACTTTATGCGCACCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGGTCTCTTCCACCGTCATCCGCGAGAAcacg                                                                                                                  >  2:1149855/1‑140 (MQ=255)
                      gcccgcccGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAgacgc                                                                                                                 >  2:1020809/1‑140 (MQ=255)
                          gcccgcTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAgacgcgacg                                                                                                             >  1:1197156/1‑140 (MQ=255)
                           cccgcTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAgacgcgacgc                                                                                                            <  2:116622/140‑1 (MQ=255)
                           cccgcTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAgacgcgacgc                                                                                                            <  2:1022917/140‑1 (MQ=255)
                               cTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCGTCCACCGTCATCCGCGAGAAGACGCGACGCCCtt                                                                                                        >  2:459537/1‑140 (MQ=255)
                                       ggCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACa                                                                                                <  2:196507/140‑1 (MQ=255)
                                         cgcTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAAt                                                                                              >  1:893884/1‑140 (MQ=255)
                                         cgcTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAAt                                                                                              <  1:270764/140‑1 (MQ=255)
                                         cgcTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAAt                                                                                              >  1:313622/1‑140 (MQ=255)
                                              aTGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCa                                                                                         >  2:524625/1‑140 (MQ=255)
                                                   gACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCt                                                                                    >  1:1215246/1‑140 (MQ=255)
                                                      gTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTcg                                                                                 >  1:1219765/1‑140 (MQ=255)
                                                        ggAAACAGCTCATACACCCTCTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTcgcg                                                                               >  1:258615/1‑140 (MQ=255)
                                                         gAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCgcgc                                                                              >  2:37988/1‑140 (MQ=255)
                                                           atcAGCTCATACACCCACTTTATGCGCTCCTGGTACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCGTTCCGGGACAATCGCCTCGGCTTCGCGCAt                                                                            <  2:1175028/138‑1 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGAttt                                                                       >  1:934831/1‑140 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGAttt                                                                       >  1:590891/1‑140 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTATCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCt                                                                                    >  1:353432/1‑127 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTATCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCt                                                                                    >  1:353438/1‑127 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTATCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCt                                                                                    <  2:353432/127‑1 (MQ=255)
                                                                cTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTATCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCt                                                                                    <  2:353438/127‑1 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  2:198123/1‑140 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  1:1125756/1‑140 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  2:623159/1‑140 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  1:1125739/1‑140 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  1:438593/1‑140 (MQ=255)
                                                                        cccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCAACCGCGAGAATACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTc                                                               >  2:179552/1‑140 (MQ=255)
                                                                        cccACTTTATGAGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAAGCGCCCATCGCCAGGTTCTATTCCACCGTCATCCGAGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGGATGATTTTCGCTGTc                                                               >  2:947348/1‑140 (MQ=255)
                                                                          cACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTg                                                             >  2:1165016/1‑140 (MQ=255)
                                                                          cACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGACAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAAACGCCACCGCTTCGCGCATGATTTTCGCTGTCTg                                                             >  2:1165212/1‑140 (MQ=255)
                                                                             tttATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCa                                                          >  2:1107880/1‑140 (MQ=255)
                                                                             tttATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCa                                                          >  2:1107952/1‑140 (MQ=255)
                                                                              atcagcgcTCCTGGAACTGGTCGCGATCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAg                                                         <  2:1219765/136‑1 (MQ=255)
                                                                               tATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGt                                                        <  1:518120/140‑1 (MQ=255)
                                                                                 tGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCg                                                      >  1:327548/1‑140 (MQ=255)
                                                                                    gcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTa                                                   <  2:538593/140‑1 (MQ=255)
                                                                                    gcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTa                                                   <  1:1084764/140‑1 (MQ=255)
                                                                                    gcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTa                                                   <  1:675887/140‑1 (MQ=255)
                                                                                     cTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCGCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTTCCAGTCGGTaa                                                  >  2:629581/1‑140 (MQ=255)
                                                                                      accTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAAt                                                 <  1:599787/139‑1 (MQ=255)
                                                                                       ccTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATg                                                >  1:1021806/1‑140 (MQ=255)
                                                                                       ccTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATg                                                >  1:253043/1‑140 (MQ=255)
                                                                                       ccTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATg                                                >  2:584755/1‑140 (MQ=255)
                                                                                       ccTGGAACTGGTCGCGATCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAAtc                                                >  2:584826/1‑139 (MQ=255)
                                                                                                ggTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTatca                                       >  2:1105353/1‑140 (MQ=255)
                                                                                                ggTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTatca                                       >  1:463852/1‑140 (MQ=255)
                                                                                                ggTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTatca                                       >  1:463865/1‑140 (MQ=255)
                                                                                                ggTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTGCGCGCATGATTTTCGCGGTCTGCCAGTCGGTCATGGCTATatcc                                       >  2:621376/1‑139 (MQ=255)
                                                                                                 gTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCAACCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTatcat                                      >  1:37985/1‑140 (MQ=255)
                                                                                                  tCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGTGTTTCGCTGTCTGACAGTCGGTAATGTCTTTatcatc                                     >  1:1228007/1‑140 (MQ=255)
                                                                                                  tCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTatcatc                                     >  2:450355/1‑140 (MQ=255)
                                                                                                     cgTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCAGCCAGTCGGTAATGTCTTTATCATCaaa                                  <  1:474284/140‑1 (MQ=255)
                                                                                                       ttCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAaca                                >  1:1079463/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  2:108887/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  2:242067/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  2:108909/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  2:108942/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  2:195362/1‑140 (MQ=255)
                                                                                                           gCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAAt                            >  1:174846/1‑140 (MQ=255)
                                                                                                             aaaacaaCCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGTAGACGCGACGCCCTTCAGTGTCAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTAGGTAATGTCTTTATCATCAAACACAATTc                          <  2:689877/140‑1 (MQ=255)
                                                                                                                aaaaCCGCCCATCGCAAGGTTCTCTTCCACCGACATCCGCGAGAATACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGAATGATTTTCGCTGTCTGCCAGTCGGTAATGTATTTATAATCAAACACAATTCGcc                       >  2:541824/1‑140 (MQ=255)
                                                                                                                   aCCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGc                    <  1:266134/140‑1 (MQ=255)
                                                                                                                   aCCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGc                    <  1:264873/140‑1 (MQ=255)
                                                                                                                             gCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCAcg          <  1:587258/140‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAATACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     >  2:606509/1‑140 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     >  2:1119861/1‑140 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     <  2:845379/140‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     <  2:845338/140‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     <  2:1113898/140‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     <  2:341572/140‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGAt     <  1:857495/140‑1 (MQ=255)
                                                                                                                                    tctcTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATcg   <  1:161215/140‑1 (MQ=255)
                                                                                                                                    tctcTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATcg   <  1:161307/140‑1 (MQ=255)
                                                                                                                                     ctctTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATcgc  >  2:172018/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
GCTGTTCACCGCCGGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCATGATTTTCGCTGTCTGCCAGTCGGTAATGTCTTTATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGC  >  NC_000913/3593008‑3593280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: