Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,923 C→A G36V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9230CA97.5% 243.6 / 0.0 79G36V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (32/45);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACT  >  NC_000913/3440790‑3441059
                                                                                                                                     |                                                                                                                                        
taccAGGACATCTTCCTGAACGCCTACTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGc                                                                                                                                    <  2:1310187/138‑1 (MQ=255)
atCCAGGACACCATCCTGAACGCCTACTGAGGAGCTGGACTCACGTCGCCCACCATCCATCACAACCTCGGTCCCCGCGCAACCCCGCACCGATGACAGCAGACGCCCGCGCAGTGCGTTACCCAGAGTAAGGACAAAGc                                                                                                                                    <  2:1499410/139‑1 (MQ=255)
  ccAGGATCTCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  2:19620/140‑1 (MQ=255)
  ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTTCTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  2:681583/140‑1 (MQ=255)
  ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  2:1222355/140‑1 (MQ=255)
  ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  2:1038896/140‑1 (MQ=255)
  ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  1:1171169/140‑1 (MQ=255)
  ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCa                                                                                                                                  <  2:681526/140‑1 (MQ=255)
       atatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGctc                                                                                                                             >  1:1185146/1‑140 (MQ=255)
          tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATTTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaa                                                                                                                          <  1:1403047/140‑1 (MQ=255)
            ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAg                                                                                                                        <  2:1225455/140‑1 (MQ=255)
                tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTc                                                                                                                    >  1:1097478/1‑140 (MQ=255)
                tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTc                                                                                                                    <  2:1066960/140‑1 (MQ=255)
                tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTc                                                                                                                    >  1:1097316/1‑140 (MQ=255)
                  aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCaa                                                                                                                  >  1:441509/1‑140 (MQ=255)
                     gCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAACGGCTCAAggg                                                                                                               <  2:548083/140‑1 (MQ=255)
                     gCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                               <  1:1241121/140‑1 (MQ=255)
                     gCCTTCTTTGGTGCGGTACTCATGTAGTACACCATCAATCTCACCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                               <  2:548001/140‑1 (MQ=255)
                            ttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                        >  1:491850/1‑140 (MQ=255)
                                 gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                   >  1:351744/1‑140 (MQ=255)
                                    gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                <  1:1006234/140‑1 (MQ=255)
                                    gTACTCATCTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGATAGCAGAATACGGCGCAGTTCGTTACCCAGAGTATGGACAAAGCCACGCTCAAAAGGCTCAAGGGTCACCTTtgcgtgcg                                                                                                >  2:1454569/1‑140 (MQ=255)
                                     tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcgt                                                                                               >  2:1303752/1‑140 (MQ=255)
                                              agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                      >  1:1506668/1‑140 (MQ=255)
                                               gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                     >  2:740461/1‑140 (MQ=255)
                                               gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                     <  1:429511/140‑1 (MQ=255)
                                               gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                     >  1:621357/1‑140 (MQ=255)
                                                  cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                  >  2:804009/1‑140 (MQ=255)
                                                   acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                 <  1:1152050/140‑1 (MQ=255)
                                                   acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                 <  1:17098/140‑1 (MQ=255)
                                                     cATCAATCTCAACCTCGGTCACCGCGCAACACGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                               >  1:412062/1‑140 (MQ=255)
                                                       tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                             >  2:1473950/1‑140 (MQ=255)
                                                       tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                             >  2:1345607/1‑140 (MQ=255)
                                                       tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGaa                                                                             >  2:1474168/1‑139 (MQ=255)
                                                        cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                            >  2:542119/1‑140 (MQ=255)
                                                        cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                            >  2:542018/1‑140 (MQ=255)
                                                                   tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                 <  1:1345607/140‑1 (MQ=255)
                                                                   tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAACACGGCGCAGTGCGTTACCCAGACGATGTACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGAGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                 <  2:1181172/140‑1 (MQ=255)
                                                                            gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                        >  1:1500288/1‑140 (MQ=255)
                                                                             cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                       <  1:917840/140‑1 (MQ=255)
                                                                                  cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                  <  2:558638/140‑1 (MQ=255)
                                                                                  cccGGCATCGATGAGAGCAGAATACGGCGCAGAGCGTTACCCAGAGTATGGACAAAGGCCCGCTCTAAAGGCTCCTGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                  <  2:718246/140‑1 (MQ=255)
                                                                                       cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTca                                             <  1:1452517/140‑1 (MQ=255)
                                                                                         tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                           <  1:420501/140‑1 (MQ=255)
                                                                                           gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                         <  2:1108005/140‑1 (MQ=255)
                                                                                           gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                         <  1:99503/140‑1 (MQ=255)
                                                                                           gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                         <  1:99415/140‑1 (MQ=255)
                                                                                                    aGAATACGGCGCTGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                <  2:260064/140‑1 (MQ=255)
                                                                                                    aGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                <  1:205174/140‑1 (MQ=255)
                                                                                                    aGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                <  2:662131/140‑1 (MQ=255)
                                                                                                    aGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                <  2:662138/140‑1 (MQ=255)
                                                                                                          cGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                          <  1:394507/140‑1 (MQ=255)
                                                                                                           ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                         >  1:488163/1‑140 (MQ=255)
                                                                                                           ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                         >  2:613570/1‑140 (MQ=255)
                                                                                                           ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                         >  2:849065/1‑140 (MQ=255)
                                                                                                           ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                         >  1:650844/1‑140 (MQ=255)
                                                                                                           ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                         >  1:56766/1‑140 (MQ=255)
                                                                                                            gcgcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                        <  1:1477284/140‑1 (MQ=255)
                                                                                                            gcgcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                        <  1:987937/140‑1 (MQ=255)
                                                                                                            gcgcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTAGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                        <  1:460324/140‑1 (MQ=255)
                                                                                                                aGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct                    >  1:1437802/1‑140 (MQ=255)
                                                                                                                aGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct                    >  2:831598/1‑140 (MQ=255)
                                                                                                                aGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct                    >  1:667495/1‑140 (MQ=255)
                                                                                                                aGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct                    >  2:831693/1‑140 (MQ=255)
                                                                                                                 gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                   <  2:825318/140‑1 (MQ=255)
                                                                                                                 gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                   <  2:1006320/140‑1 (MQ=255)
                                                                                                                 gTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                   <  2:825298/140‑1 (MQ=255)
                                                                                                                  tGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTGTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                  >  1:149119/1‑140 (MQ=255)
                                                                                                                    cGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                <  1:1272668/140‑1 (MQ=255)
                                                                                                                       tACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                        <  2:706764/129‑1 (MQ=255)
                                                                                                                       tACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                        >  1:706764/1‑129 (MQ=255)
                                                                                                                        aCCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt            <  2:1322492/140‑1 (MQ=255)
                                                                                                                            agagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc        >  2:984387/1‑140 (MQ=255)
                                                                                                                            agagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc        >  2:1254786/1‑140 (MQ=255)
                                                                                                                            agagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCGCTGTTTGGGACTAAGc        >  2:65412/1‑140 (MQ=255)
                                                                                                                             gagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCTAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt       <  1:45606/140‑1 (MQ=255)
                                                                                                                                  tGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt  <  1:446423/140‑1 (MQ=255)
                                                                                                                                  tGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt  <  1:1130921/140‑1 (MQ=255)
                                                                                                                                  tGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt  <  1:1048966/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACT  >  NC_000913/3440790‑3441059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: