| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,630,528 | T→G | Y129S (TAT→TCT) | ydfI ← | putative NAD‑dependent D‑mannonate oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,630,528 | 0 | T | G | 93.2% | 149.7 / 0.2 | 44 | Y129S (TAT→TCT) | ydfI | putative NAD‑dependent D‑mannonate oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (16/25); total (16/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
AAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTC > NC_000913/1630392‑1630656 | aaGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCCAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGAcc < 1:987772/140‑1 (MQ=255) ttACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATAGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGAt > 2:106888/1‑140 (MQ=255) ccTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATc > 1:207630/1‑140 (MQ=255) aTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACACTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg < 1:1164264/140‑1 (MQ=255) tCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAGTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTGTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGc > 1:1295115/1‑140 (MQ=255) aCCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg > 1:584148/1‑140 (MQ=255) aCCCCTGGTGCTGTTTTCGGCTGGGGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg > 2:1359232/1‑140 (MQ=255) tGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcac > 2:642146/1‑140 (MQ=255) cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca < 2:243950/140‑1 (MQ=255) cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca < 1:405479/140‑1 (MQ=255) cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca < 1:395817/140‑1 (MQ=255) cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca < 2:1373890/140‑1 (MQ=255) cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca < 2:1373929/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 1:1446920/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 1:1406989/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 2:148586/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 2:334673/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 2:334532/140‑1 (MQ=255) tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGGGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg < 2:511300/140‑1 (MQ=255) tGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGcc > 1:1044289/1‑140 (MQ=255) cACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCat < 2:642808/140‑1 (MQ=255) cACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCat < 2:49186/140‑1 (MQ=255) cAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGt < 1:1028479/140‑1 (MQ=255) ccATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGc < 1:543083/140‑1 (MQ=255) cATCGGGTGATCGAGCATTAACTGTCCTGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGATACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCa < 2:1193060/140‑1 (MQ=255) cATCGGGTGATCGAGCATTAACTGTCCTGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGATACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCa < 2:834852/140‑1 (MQ=255) gTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGTAAGACCCGTTTTCGGTGATTGTCAGAGAGACTATCGCGATTTGCGGTTCACACATCGCTGCCGACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtt > 2:743518/1‑140 (MQ=255) gTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtt > 2:113558/1‑140 (MQ=255) gATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtttt < 2:1379661/140‑1 (MQ=255) aGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAac < 1:1213337/140‑1 (MQ=255) cATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacga > 1:42650/1‑140 (MQ=255) tacGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacga > 1:212078/3‑140 (MQ=255) tCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacga > 1:1032844/1‑140 (MQ=255) tCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacga > 1:536285/1‑140 (MQ=255) tCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCAGCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacga > 1:1032954/1‑140 (MQ=255) tCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATAGCGATTTGCGGTTCACACATCGCAGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacga > 1:1500510/1‑140 (MQ=255) cGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacgacg > 2:23235/1‑140 (MQ=255) ggTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGTCAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgc < 1:84564/140‑1 (MQ=255) ggTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgc < 1:948092/140‑1 (MQ=255) ggTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgc < 1:84472/140‑1 (MQ=255) cGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAg < 1:186431/140‑1 (MQ=255) gCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGc < 1:1243082/140‑1 (MQ=255) gagaGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTc < 2:212078/140‑1 (MQ=255) gagaGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTc < 2:777903/140‑1 (MQ=255) | AAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTC > NC_000913/1630392‑1630656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |