Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,020,473 C→A N129K (AAC→AAA fliM → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,020,4730CA97.2% 217.2 / 0.1 73N129K (AAC→AAAfliMflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (36/33);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CGCATTCAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGC  >  NC_000913/2020339‑2020609
                                                                                                                                      |                                                                                                                                        
cGCATTCAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGtt                                                                                                                                     <  2:972425/140‑1 (MQ=255)
cGCATTCAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGtt                                                                                                                                     <  2:974020/140‑1 (MQ=255)
      cAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTAggcgg                                                                                                                               >  2:1070354/1‑140 (MQ=255)
      cAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCAGCGCGGCACTGGGCAGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTggcgg                                                                                                                               >  2:1071525/1‑140 (MQ=255)
            tACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATgg                                                                                                                         >  1:710404/1‑140 (MQ=255)
            tACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATgg                                                                                                                         >  1:434592/1‑140 (MQ=255)
             aCCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGa                                                                                                                        <  2:1173997/140‑1 (MQ=255)
             aCCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGa                                                                                                                        <  2:1063838/140‑1 (MQ=255)
             aCCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGa                                                                                                                        <  2:1062898/140‑1 (MQ=255)
                 tGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCt                                                                                                                    >  2:1352665/1‑140 (MQ=255)
                         cccGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGAcc                                                                                                            >  1:1048143/1‑140 (MQ=255)
                         cccGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGAcc                                                                                                            >  1:180460/1‑140 (MQ=255)
                         cccGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGAcc                                                                                                            >  1:1048170/1‑140 (MQ=255)
                         cccGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGAcc                                                                                                            >  1:180360/1‑140 (MQ=255)
                                ccTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTgg                                                                                                     <  1:1526064/140‑1 (MQ=255)
                                 cTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGa                                                                                                    <  2:702024/140‑1 (MQ=255)
                                       gTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTcg                                                                                              <  1:119799/140‑1 (MQ=255)
                                           cGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAg                                                                                          <  1:257784/140‑1 (MQ=255)
                                           cGACCAAACTGAACCTTATCCATCTGAAACCTCTGCGCGTCACTGGGCTGTTGGTGTTCTCACCGAGTCTGGAGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGTAGGTCGCGAg                                                                                          <  2:428501/140‑1 (MQ=255)
                                            gACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGt                                                                                         <  2:916877/140‑1 (MQ=255)
                                            gACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGt                                                                                         <  2:916812/140‑1 (MQ=255)
                                                aaCCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTAc                                                                                     <  1:36864/140‑1 (MQ=255)
                                                aaCCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTAc                                                                                     <  2:732639/140‑1 (MQ=255)
                                                aaCCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTAc                                                                                     <  2:732626/140‑1 (MQ=255)
                                                aaCCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTAc                                                                                     <  1:879434/140‑1 (MQ=255)
                                                     gAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATa                                                                                <  2:241908/140‑1 (MQ=255)
                                                     gAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATa                                                                                <  2:241458/140‑1 (MQ=255)
                                                        ccTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCg                                                                             >  1:683851/1‑140 (MQ=255)
                                                        ccTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCg                                                                             >  1:683483/1‑140 (MQ=255)
                                                           tATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAAc                                                                          >  1:1377894/1‑140 (MQ=255)
                                                            aTCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACa                                                                         >  2:834927/1‑140 (MQ=255)
                                                            aTCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACa                                                                         >  2:624264/1‑140 (MQ=255)
                                                             tCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAg                                                                        >  1:843033/1‑140 (MQ=255)
                                                              ccATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgc                                                                       <  1:58878/140‑1 (MQ=255)
                                                              ccATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgc                                                                       <  1:1157419/140‑1 (MQ=255)
                                                                aTCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgcgc                                                                     >  1:825755/1‑140 (MQ=255)
                                                                aTCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgcgc                                                                     >  2:1184894/1‑140 (MQ=255)
                                                                aTCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgcgc                                                                     >  1:825817/1‑140 (MQ=255)
                                                                aTCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGATTATCGCCGTGGATAAACTGTTAGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAgcgc                                                                     >  1:824017/1‑140 (MQ=255)
                                                                 tCTGAACCCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGCGTCTGGTGTTTATCGCCGTGGATAAACTGTGTGGAGGCGATGGACGCGTCCCGACCACAGTGGAAGGTCGCGTGTTTACCCATACCGAACAGcgcg                                                                    <  2:1223486/140‑1 (MQ=255)
                                                                  cTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGt                                                                   >  1:391889/1‑140 (MQ=255)
                                                                     aaaCCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAATCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGtcat                                                                >  2:440508/1‑140 (MQ=255)
                                                                                        ggCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAATGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTg                                             >  1:82036/1‑140 (MQ=255)
                                                                                        ggCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTg                                             >  1:81975/1‑140 (MQ=255)
                                                                                             gtggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCt                                        >  2:1165917/1‑140 (MQ=255)
                                                                                             gtggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCt                                        >  2:1165954/1‑140 (MQ=255)
                                                                                               ggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTg                                      <  2:901688/140‑1 (MQ=255)
                                                                                               ggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTg                                      <  2:900690/140‑1 (MQ=255)
                                                                                               ggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTg                                      <  2:450495/140‑1 (MQ=255)
                                                                                               ggtgTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTg                                      <  2:900568/140‑1 (MQ=255)
                                                                                                 tgtTCTAACCGAGTCTGGTGTTAATCGCCTTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGAGCACAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGaa                                    <  2:101948/140‑1 (MQ=255)
                                                                                                  gtTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAg                                   >  1:102839/1‑140 (MQ=255)
                                                                                                  gtTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAg                                   >  2:358231/1‑140 (MQ=255)
                                                                                                  gtTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAg                                   >  2:805370/1‑140 (MQ=255)
                                                                                                   ttCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACACATACCGAACAGCGCGTCATCAACCGCATGTTGACACTGGCGCTTGAAgg                                  >  1:1130813/1‑140 (MQ=255)
                                                                                                   ttCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACACATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAgg                                  >  1:751906/1‑140 (MQ=255)
                                                                                                   ttCTCACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACACATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAgg                                  >  1:1130737/1‑140 (MQ=255)
                                                                                                       cACCGAGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCtat                              >  1:374311/1‑140 (MQ=255)
                                                                                                            aGTCTTGTGTTTATAGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTAACCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCAATAGCGa                         <  2:339644/140‑1 (MQ=255)
                                                                                                            aGTCTGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGa                         <  2:237249/140‑1 (MQ=255)
                                                                                                            aGTCTGGTGTTTATCGCCGTGGATAAACTGTGTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGa                         <  2:237415/140‑1 (MQ=255)
                                                                                                            aGTCTGGTGTTTATAGCCGCGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGTCCAAAGTGGTAGGTCGCGAGTTTACCCATACCGAAAAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGa                         <  2:339462/140‑1 (MQ=255)
                                                                                                                tGGTGTTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCCTACCGAACAGCGCGTCATCAACCGCCTGTTGAAACTGGCGCTTGAAGGCTATAGCGACGcc                     >  2:786268/1‑140 (MQ=255)
                                                                                                                  gtgtTTATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTg                   >  2:1076525/1‑140 (MQ=255)
                                                                                                                       tATCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAgg              >  2:596763/1‑140 (MQ=255)
                                                                                                                        aTCGCCGTGGATAATCAGTTTGGCGGCGATGGACGCTTCCCGTCCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCGATTGCGACGCCTGGAAGGc             >  2:1364317/1‑140 (MQ=255)
                                                                                                                        aTCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGc             >  2:980634/1‑140 (MQ=255)
                                                                                                                        aTCGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGc             >  2:265732/1‑140 (MQ=255)
                                                                                                                          cGCCGTGGATTAACTGTTTGGCGGCGATGGACGCGTCCCGACCAATGTGGCTGGTCGCGAGTTTACCCCTACCGAACCGCGCGTGTTCAACCGCTTGTTGTTTCTGGCGCTTGAAGGCTCTTGCGTGGCCTGGTAGGCgt           <  2:641740/140‑2 (MQ=255)
                                                                                                                          cGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCATGAAGGCTATAGCGACGCCTGGAAGGCGa           <  2:11268/140‑1 (MQ=255)
                                                                                                                          cGCCGTGGATAAACTGTTTGGCGGCGATGGACGCTTCCCGACAAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGa           <  2:641676/140‑1 (MQ=255)
                                                                                                                            ccGTGGATAAACAGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGGATGATGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGAtt         >  1:743122/1‑140 (MQ=255)
                                                                                                                                   tAAACTGTTTGGCGGCGATGGACGCTTCCAGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGc  <  1:1366429/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CGCATTCAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATCCATCTGAAACCGCTGCGCGGCACTGGGCTGGTGGTGTTCTCACCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGC  >  NC_000913/2020339‑2020609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: