Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC23.7% 373.1 / 120.7 169intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (67/62);  new base C (19/21);  total (86/83)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

CCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865583‑1865781
                                                                                                   |                                                                                                   
cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTg                                                                                                     >  1:3095786/1‑100 (MQ=255)
      ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                               <  2:209268/100‑1 (MQ=255)
        cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTg                                                                                             <  1:2479976/100‑1 (MQ=255)
        cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTg                                                                                             <  2:225795/100‑1 (MQ=255)
          ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                           >  2:210151/1‑100 (MQ=255)
            cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  2:3125953/100‑1 (MQ=255)
            cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  1:420467/100‑1 (MQ=255)
            cAGGGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTAGTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  2:2438482/100‑1 (MQ=255)
             agggagaCACCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  2:1830728/97‑2 (MQ=39)
             aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  1:2882091/100‑2 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      <  2:725778/100‑1 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      <  2:725440/100‑1 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      >  1:1639552/1‑100 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      >  1:1639563/1‑100 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  1:2696389/100‑4 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  2:178845/100‑4 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  2:421765/100‑4 (MQ=255)
                gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  2:2931735/100‑1 (MQ=255)
                gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgcat                                                                                     <  1:54861/100‑5 (MQ=255)
                  naCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                   >  1:2000901/2‑100 (MQ=255)
                  gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                   >  1:2001033/1‑100 (MQ=255)
                  gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                   >  1:2948236/1‑100 (MQ=255)
                   aCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTGCTGCCGGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcac                                                                                  <  1:1046415/100‑1 (MQ=255)
                       cGCCACTAAATTGAATCATTTTTTGTTTCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                              >  2:1566120/1‑100 (MQ=255)
                       cGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                              >  2:538759/1‑100 (MQ=255)
                           aCTAAATTGAATCATTTTTTGTTTCATTGTTTCCTCCCTTTTCTTGCACCACCGTAATGCATTACGGTCCGTGACGCCCTGTTGTCATCCACACATTGcc                                                                          >  2:1846008/1‑100 (MQ=255)
                            cTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                         <  1:2312181/100‑1 (MQ=255)
                            cTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                         <  1:846336/100‑1 (MQ=255)
                             tAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                        <  1:63867/100‑1 (MQ=255)
                               aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCCCCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                      <  2:1871467/100‑1 (MQ=255)
                                aTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGa                                                                     >  1:2947568/1‑100 (MQ=255)
                                  tGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                   <  1:1186171/100‑1 (MQ=255)
                                  tGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                   <  1:1185995/100‑1 (MQ=255)
                                   gAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAg                                                                  >  2:543311/1‑100 (MQ=255)
                                     aTCATTTTTTTTTTTTTTTTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGTAAAGAt                                                                >  2:1060576/1‑100 (MQ=255)
                                     aTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                >  1:534611/1‑100 (MQ=255)
                                     aTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                >  1:2433625/1‑100 (MQ=255)
                                      tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                               <  1:3099444/100‑1 (MQ=255)
                                        aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                             >  2:1693928/1‑100 (MQ=255)
                                         ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAt                                                            <  2:193499/100‑1 (MQ=255)
                                            tttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAt                                                         <  2:617883/100‑1 (MQ=255)
                                               gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                      <  1:996043/100‑1 (MQ=255)
                                                 tgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                    >  1:660935/1‑100 (MQ=255)
                                                 tgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                    >  2:33266/1‑100 (MQ=255)
                                                   cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                  >  2:1408807/1‑100 (MQ=255)
                                                    aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAttt                                                 >  2:555943/1‑100 (MQ=255)
                                                    aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAttt                                                 <  2:1747624/100‑1 (MQ=255)
                                                     ttGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                <  2:807604/100‑1 (MQ=255)
                                                     ttGTCTCCGCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                <  2:1588250/100‑1 (MQ=255)
                                                       gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                              >  1:1023065/1‑100 (MQ=255)
                                                        tttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                             <  2:1975723/100‑1 (MQ=255)
                                                         ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                            <  2:1844398/100‑1 (MQ=255)
                                                            ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                         >  2:1861407/1‑100 (MQ=255)
                                                              cccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATc                                       >  1:1338899/1‑100 (MQ=255)
                                                                cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                     >  2:2692861/1‑100 (MQ=255)
                                                                cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                     >  2:1053185/1‑100 (MQ=255)
                                                                    tATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTa                                 <  2:1524578/100‑1 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                <  2:2339263/100‑1 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                >  1:431239/1‑100 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                <  2:3036334/100‑1 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                >  1:1244913/1‑100 (MQ=255)
                                                                      ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                               <  2:702482/100‑1 (MQ=255)
                                                                        gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTCTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                             >  2:666909/1‑100 (MQ=255)
                                                                         caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAAt                            >  2:2819294/1‑100 (MQ=255)
                                                                         caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAAt                            >  2:1730563/1‑100 (MQ=255)
                                                                          accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                           >  1:1761862/1‑100 (MQ=255)
                                                                          accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                           >  1:3049460/1‑100 (MQ=255)
                                                                           ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTCTCAGAATTTGTAGGTGATCCCGGTAGAAATCa                          >  2:2194793/1‑100 (MQ=255)
                                                                             accGTAATGCAGAACAGGGTGTGTCGCCCTGTTTTTATGCACAGAATGCCCTGGAAAGATGCGTTATCAGGATTTGTAGGTGATCCCGGTAGAAATCAgg                        <  1:1388465/100‑1 (MQ=255)
                                                                              ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                       >  1:1473705/1‑100 (MQ=255)
                                                                              ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                       >  2:1401504/1‑100 (MQ=255)
                                                                               cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                      <  2:3107609/100‑1 (MQ=255)
                                                                               cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                      <  2:2947567/100‑1 (MQ=255)
                                                                               cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                      <  2:155617/100‑1 (MQ=255)
                                                                                gTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                     >  2:2536546/1‑100 (MQ=255)
                                                                                 tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                    <  2:2480640/100‑1 (MQ=255)
                                                                                  gcataaaaaCAGGNCGTCACGCNCTGTTTTTATGCACAAAATGCCCTGGNAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  2:929657/6‑100 (MQ=255)
                                                                                  aaTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   <  2:542683/100‑1 (MQ=255)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCCCAAAATGCCCTGGAAAGCTGCCTTATCACCATCTGTAGCTGGTCCCGGTCCACATCACGCCCGTc                  >  2:3117239/5‑100 (MQ=16)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:2210356/5‑100 (MQ=255)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:886358/5‑100 (MQ=255)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:2620649/5‑100 (MQ=255)
                                                                                    tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 <  1:2178827/100‑1 (MQ=255)
                                                                                    ataaaaaCAGGGTGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 >  2:2241443/4‑100 (MQ=255)
                                                                                    ataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 >  2:2558124/4‑100 (MQ=255)
                                                                                     gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  2:1081847/1‑100 (MQ=255)
                                                                                       aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATNCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              >  2:291719/1‑100 (MQ=255)
                                                                                       aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              >  2:516122/1‑100 (MQ=255)
                                                                                        aaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa             >  2:594785/1‑100 (MQ=255)
                                                                                         aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGCTGCATTATCAGAATTTGTAGGTGATCGCGGTAGAAATCAGGCCTGTCCAGGAt            >  2:2060066/1‑100 (MQ=255)
                                                                                         aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            >  1:1073053/1‑100 (MQ=255)
                                                                                         aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            >  2:2623298/1‑100 (MQ=255)
                                                                                          aCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt           >  2:2761324/1‑100 (MQ=255)
                                                                                           cAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAttn          <  2:1069021/100‑2 (MQ=255)
                                                                                             gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt        <  1:194605/100‑1 (MQ=255)
                                                                                             gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt        >  1:976642/1‑100 (MQ=255)
                                                                                              ggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATc       <  1:1545058/100‑1 (MQ=255)
                                                                                                cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca     >  2:1212381/1‑100 (MQ=255)
                                                                                                  tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc   >  1:2034785/1‑100 (MQ=255)
                                                                                                  tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc   >  1:2820992/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865583‑1865781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: