BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,286,315	0	C	G	23.4%	308.1 / 97.4	137	intergenic (+28/+75)	proL/yejO	tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base C (48/57); new base G (16/16); total (64/73)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 139 total aligned reads displayed.

CGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCG  >  NC_000913/2286216‑2286412

cGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                   >  1:1534322/1‑100 (MQ=255)

 gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACg                                                                                                  <  2:1014574/100‑1 (MQ=255)

   aGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                                >  1:2543365/1‑100 (MQ=255)

   aGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                                <  2:1416985/100‑1 (MQ=255)

    gcgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggtt                                                                                               <  2:2179730/100‑1 (MQ=255)

     cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                              >  1:668600/1‑100 (MQ=255)

     cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                              <  1:1664874/100‑1 (MQ=255)

     cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                              <  1:1664769/100‑1 (MQ=255)

     cgcAGCCTGGTAGAGCACCGTCATGGGGTGTCGGGGGGCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCAAAAGAAAAAACCAACCCTTACggttg                                                                                              <  2:2370701/100‑1 (MQ=255)

       cAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                            <  2:2282231/100‑1 (MQ=255)

          ccTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttt                                                                                         <  2:521013/100‑1 (MQ=39)

           cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAGGCATCCCAAGCAAAAACCAACCCTCACGGTTGGctttt                                                                                        >  2:2396653/1‑100 (MQ=255)

           cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttt                                                                                        <  2:1709616/100‑1 (MQ=39)

           cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttt                                                                                        <  2:1709632/100‑1 (MQ=39)

           cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                        >  2:387591/1‑100 (MQ=255)

            tGGCAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                       >  1:164790/1‑100 (MQ=255)

             ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                      <  1:2794441/100‑1 (MQ=255)

             ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                      <  2:2030639/100‑1 (MQ=255)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAGTCCCAAGAAAAAACCAACCCTTACGGTGGGTTTTTTta                                                                                     <  2:2958235/100‑1 (MQ=255)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  2:924439/100‑3 (MQ=38)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  2:723015/100‑3 (MQ=38)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  1:1885543/100‑3 (MQ=38)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  1:3182960/100‑3 (MQ=38)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  2:723054/100‑3 (MQ=38)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                     <  1:1639388/100‑1 (MQ=255)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCACTCGGGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                     <  2:2331444/100‑3 (MQ=37)

              gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGCTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                     <  1:1788684/100‑1 (MQ=255)

              gTAGCGAACCGTCATGGGGTGTCGGGGGTCGGAGGTGCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                     <  2:1958406/100‑1 (MQ=255)

                aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttg                                                                                   <  2:815626/100‑5 (MQ=37)

                aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttg                                                                                   <  2:897468/100‑5 (MQ=38)

                aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                   <  1:1281819/100‑1 (MQ=255)

                aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACAAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttg                                                                                   <  2:2105903/100‑5 (MQ=37)

                          cATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatta                                                                         >  2:2673398/1‑100 (MQ=255)

                            tGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATACCTGCaattaat                                                                       >  1:3078120/1‑100 (MQ=255)

                            tGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTATTTATATCTGCaattaat                                                                       >  1:1004528/1‑100 (MQ=255)

                             ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                      >  1:2538262/1‑100 (MQ=255)

                             ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                      >  1:1864946/1‑100 (MQ=255)

                              gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                                     >  1:2133938/1‑100 (MQ=255)

                               ggTGTCGGGGGTCGGAGGTTCAAGTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAAGTAATTCg                                                                    <  2:3105518/100‑1 (MQ=255)

                               ggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                    <  1:2678007/100‑1 (MQ=255)

                               ggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                    <  1:34652/100‑1 (MQ=255)

                               ggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                    <  1:2127758/100‑1 (MQ=255)

                                   tCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaa                                                                <  1:1771606/100‑1 (MQ=255)

                                      ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                             <  1:228795/100‑1 (MQ=255)

                                      ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                             <  1:228666/100‑1 (MQ=255)

                                      ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                             <  1:979708/100‑1 (MQ=255)

                                          tCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGAcc                                                         >  2:87702/1‑100 (MQ=255)

                                             gAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg                                                      >  2:1597970/1‑100 (MQ=255)

                                             gAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg                                                      <  2:957836/100‑1 (MQ=255)

                                               ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGac                                                    <  1:1083862/100‑1 (MQ=255)

                                               ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGac                                                    >  1:1192141/1‑100 (MQ=255)

                                               ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGac                                                    <  1:2144881/100‑1 (MQ=255)

                                               ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTATATATCTGCAATTAATTCGATAAACAGACCGTGac                                                    >  1:1074357/1‑100 (MQ=255)

                                                 ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGacac                                                  >  2:114662/1‑100 (MQ=255)

                                                 ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGacac                                                  >  2:886764/1‑100 (MQ=255)

                                                 ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca                                                 <  1:48971/100‑1 (MQ=255)

                                                  tCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGCTAAACAGACCGTGAcaca                                                 >  2:2870148/1‑100 (MQ=255)

                                                  tCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca                                                 <  1:963746/100‑1 (MQ=255)

                                                   aaaaaCCTCTCGTGGCGACCAAAAATCCCAAGAAAAAACCAACCCTGACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAGCAGACCGTGACACAt                                                <  2:2019707/99‑1 (MQ=255)

                                                    aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATc                                               <  1:1994382/100‑1 (MQ=255)

                                                       tCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca                                            <  2:948592/100‑1 (MQ=255)

                                                       tCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAg                                           >  1:3117463/1‑100 (MQ=255)

                                                       nccTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca                                            >  1:2047142/2‑100 (MQ=255)

                                                                gCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTa                                   <  1:1379998/100‑1 (MQ=255)

                                                                 ccGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAt                                  >  2:1619162/1‑100 (MQ=255)

                                                                    aCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtttt                               >  2:2813813/1‑100 (MQ=255)

                                                                      cAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                             >  2:1233617/1‑100 (MQ=255)

                                                                       aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                            <  1:1462441/100‑1 (MQ=255)

                                                                       aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                            <  1:1417411/100‑1 (MQ=255)

                                                                       aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                            <  1:1542169/100‑1 (MQ=255)

                                                                       aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                            <  1:384346/100‑1 (MQ=255)

                                                                          aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                         >  2:2360236/1‑100 (MQ=255)

                                                                          aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                         <  1:2624622/100‑1 (MQ=255)

                                                                          aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAAGTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                         <  2:1907341/100‑1 (MQ=255)

                                                                             cccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                      >  1:1560970/1‑100 (MQ=255)

                                                                              ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                     >  1:370159/1‑100 (MQ=255)

                                                                              ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                     >  2:3131636/1‑100 (MQ=255)

                                                                              ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                     >  1:1731504/1‑100 (MQ=255)

                                                                               cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                    >  2:1368807/1‑100 (MQ=255)

                                                                               cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                    >  1:555941/1‑100 (MQ=255)

                                                                               cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                    >  1:192893/1‑100 (MQ=255)

                                                                                ataaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGaa                   >  2:556087/4‑100 (MQ=38)

                                                                                 taaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc                  >  1:64023/3‑100 (MQ=38)

                                                                                  gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                 <  1:1972084/100‑1 (MQ=255)

                                                                                  gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                 >  2:2495985/1‑100 (MQ=255)

                                                                                  gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                 >  1:1271706/1‑100 (MQ=255)

                                                                                  aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACCGCCTGTTTATTCTCTGTTATCAGAACg                 >  2:3200799/2‑100 (MQ=38)

                                                                                  aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                 >  2:3200975/2‑100 (MQ=38)

                                                                                   aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGt                >  1:967331/1‑100 (MQ=39)

                                                                                    aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc               <  2:672517/100‑1 (MQ=255)

                                                                                    aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc               <  2:2275643/100‑1 (MQ=255)

                                                                                     aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc              >  2:602653/1‑100 (MQ=39)

                                                                                     aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc              >  2:602652/1‑100 (MQ=39)

                                                                                     aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc              >  2:600979/1‑100 (MQ=39)

                                                                                      aaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCa             <  2:2344160/100‑1 (MQ=255)

                                                                                         ccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAgcc          <  2:1805176/100‑3 (MQ=255)

                                                                                             cccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACcaca      >  1:805284/1‑100 (MQ=255)

                                                                                              ccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac     >  2:2322665/1‑100 (MQ=255)

                                                                                                ttACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACAccc   <  1:2095686/100‑1 (MQ=255)

                                                                                                 tACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCg  <  2:2210910/100‑1 (MQ=255)

CGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCG  >  NC_000913/2286216‑2286412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`