Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7370TC23.2% 241.7 / 73.5 125intergenic (+26/‑167)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base T (51/45);  new base C (10/19);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 9.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 132 total aligned reads displayed.

AATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACT  >  NC_000913/2356638‑2356836
                                                                                                   |                                                                                                   
aaTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCAt                                                                                                     >  2:725960/1‑100 (MQ=255)
 aTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCAtg                                                                                                    <  1:954216/100‑1 (MQ=255)
    gCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                 <  2:245136/100‑1 (MQ=255)
       gATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGttc                                                                                              >  2:651979/1‑99 (MQ=255)
          tGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCg                                                                                           >  1:1485715/1‑100 (MQ=255)
           gagaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCgg                                                                                          <  1:889597/100‑1 (MQ=255)
           gagaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                          <  2:3100993/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCggg                                                                                         <  1:2830829/100‑1 (MQ=255)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCggg                                                                                         <  1:2303121/100‑1 (MQ=255)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  2:750379/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  1:3042154/100‑1 (MQ=39)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  1:2245310/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  2:1044294/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  1:1266353/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  1:1266033/100‑1 (MQ=38)
            agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                         <  1:1266221/100‑1 (MQ=38)
             gaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGc                                                                                        <  1:847743/100‑1 (MQ=38)
              aTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCa                                                                                       <  1:613327/100‑1 (MQ=38)
              aTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCa                                                                                       <  1:613436/100‑1 (MQ=38)
               tGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAt                                                                                      >  1:2472050/1‑100 (MQ=255)
                 tCCACAAGTCTTCGCTGCGAACATCCGATTACGCGACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCAtca                                                                                    <  1:1785626/100‑3 (MQ=37)
                  ccACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                   >  2:1284094/1‑100 (MQ=255)
                  ccACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                   >  2:1283895/1‑100 (MQ=255)
                   cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttttt                                                                                  >  2:2244219/1‑100 (MQ=255)
                   cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttct                                                                                  >  1:264091/1‑98 (MQ=255)
                   cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCCTAAACTCCCTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCCttttt                                                                                  >  2:1251006/1‑100 (MQ=255)
                    acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                 >  1:757198/1‑100 (MQ=255)
                      aaGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                               <  2:100956/100‑1 (MQ=255)
                          cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                           >  1:1133722/1‑100 (MQ=255)
                          cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                           <  2:3138981/100‑1 (MQ=255)
                          cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                           >  1:1133712/1‑100 (MQ=255)
                          cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACACCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                           >  1:1133540/1‑100 (MQ=255)
                            tCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACg                                                                         <  1:85139/100‑1 (MQ=255)
                                  cGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCg                                                                   <  2:3204419/100‑1 (MQ=255)
                                    aaCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTg                                                                 <  1:1472352/100‑1 (MQ=255)
                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                <  1:1483147/100‑1 (MQ=255)
                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                <  1:1473092/100‑1 (MQ=255)
                                      cATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                               >  2:1383771/1‑100 (MQ=255)
                                       aTCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGccc                                                              <  2:251413/100‑1 (MQ=255)
                                         ccGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCaa                                                            >  2:1658555/1‑100 (MQ=255)
                                         ccGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCaa                                                            >  1:598159/1‑100 (MQ=255)
                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAAt                                                           >  1:345757/1‑100 (MQ=255)
                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAAt                                                           >  1:345256/1‑100 (MQ=255)
                                               aCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                      <  1:76890/100‑1 (MQ=255)
                                               aCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                      >  1:2977190/1‑100 (MQ=255)
                                                 gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCg                                                    <  2:3192225/100‑1 (MQ=255)
                                                 gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCg                                                    <  2:3192617/100‑1 (MQ=255)
                                                  cTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGt                                                   >  2:1894468/1‑100 (MQ=255)
                                                  cTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGt                                                   >  2:894416/1‑100 (MQ=255)
                                                  cTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCACGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGt                                                   >  2:2515684/1‑100 (MQ=255)
                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTg                                                 <  1:1832098/100‑1 (MQ=255)
                                                       ggCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCaa                                              >  2:172746/1‑100 (MQ=255)
                                                       ggCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCaa                                              >  2:1670066/1‑100 (MQ=255)
                                                        gCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAAc                                             <  2:1981138/100‑1 (MQ=255)
                                                           cAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                          >  1:1509444/1‑100 (MQ=255)
                                                            aGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAg                                         <  2:2268099/100‑1 (MQ=255)
                                                            aGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAg                                         <  2:691490/100‑1 (MQ=255)
                                                                 cTGGCTTTAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGaaa                                    <  2:1597415/100‑1 (MQ=255)
                                                                  tGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGaaaa                                   <  1:131827/100‑1 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                  >  1:2491033/1‑100 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                  >  1:2490960/1‑100 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                  <  1:2994296/100‑1 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                  >  1:1932385/1‑100 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                  >  1:2801582/1‑100 (MQ=255)
                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGCGAAAAt                                  >  2:2611093/1‑100 (MQ=255)
                                                                     cTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                >  2:1469836/1‑100 (MQ=255)
                                                                     cTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                >  1:1689024/1‑100 (MQ=255)
                                                                        aaaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGAAAAGAAAATTCctc                             <  2:647738/100‑1 (MQ=255)
                                                                         aaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                            <  2:2173959/100‑1 (MQ=255)
                                                                         aaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                            >  2:554120/1‑100 (MQ=255)
                                                                          aCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTg                           <  2:2445144/100‑1 (MQ=255)
                                                                             ccTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaaa                        <  2:2537163/100‑1 (MQ=255)
                                                                              cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTTTGaaaa                       <  2:1646702/100‑1 (MQ=255)
                                                                               tttCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                      <  1:1904003/100‑1 (MQ=255)
                                                                               tttCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                      <  1:2672991/100‑1 (MQ=255)
                                                                                ttCTGATGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGTGTACACGATTCCGTGCCCACTGTATGCGTTGCAACGCAGTCAAAATTCCTCTGaacgcg                     >  2:2873923/1‑96 (MQ=255)
                                                                                ttCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACg                     <  1:2418887/100‑1 (MQ=255)
                                                                                ttCTGATGCCCGGTAAGCATGTGGTTAACGGGCATTTTTGTGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTAAAAATTCCTCTGAACACg                     >  2:2872693/1‑100 (MQ=255)
                                                                                  aaaaTGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtc                   >  1:2246389/4‑100 (MQ=37)
                                                                                  aaaaTGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtc                   >  2:2817068/4‑100 (MQ=37)
                                                                                    gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtctc                 >  1:2862655/1‑100 (MQ=255)
                                                                                     aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCg                <  2:898450/100‑1 (MQ=255)
                                                                                      tGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGc               >  1:1618420/1‑100 (MQ=38)
                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa              >  1:2441321/1‑100 (MQ=255)
                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa              >  2:1551958/1‑100 (MQ=38)
                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa              >  2:1551859/1‑100 (MQ=38)
                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa              >  2:436039/1‑100 (MQ=38)
                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa              >  2:80602/1‑100 (MQ=38)
                                                                                        cccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCaa             >  2:1360575/1‑100 (MQ=255)
                                                                                            gTAAGCATGTGGTTACCGGGCATTTTTTTTTTTTTTTTTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTGCTCTGAAAACGTCTCGCACAGGc         >  2:1028625/1‑100 (MQ=255)
                                                                                            gTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc         <  2:475750/100‑1 (MQ=255)
                                                                                            gTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc         <  1:1021300/100‑1 (MQ=255)
                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCt        >  1:2845760/1‑100 (MQ=255)
                                                                                              aaGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTg       <  1:2818714/100‑1 (MQ=255)
                                                                                              aaGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTg       <  2:2378000/100‑1 (MQ=255)
                                                                                                gCATGTGGTTACCGGGCATTTTTTTTTTTTTTTTTCCGTGCCCAATGTATGCGTTGCATCGCAGTGTAATTTCCTCTGAAAACGTCTCGCACAGGCTGaa     >  2:3091301/1‑100 (MQ=255)
                                                                                                gCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaa     <  1:393831/100‑1 (MQ=255)
                                                                                                gCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaa     <  1:2512013/100‑1 (MQ=255)
                                                                                                gCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaa     >  2:750762/1‑100 (MQ=255)
                                                                                                   tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt  <  1:654305/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACT  >  NC_000913/2356638‑2356836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: