Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,2900TC23.5% 246.8 / 76.9 133G170G (GGA→GGG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base T (40/61);  new base C (11/20);  total (51/82)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 136 total aligned reads displayed.

TTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAG  >  NC_000913/4307193‑4307389
                                                                                                 |                                                                                                   
ttCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTcc                                                                                                   <  1:1614046/100‑1 (MQ=255)
  cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                                                                                 >  1:2856598/1‑100 (MQ=255)
  cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                                                                                 >  1:1996060/1‑100 (MQ=255)
  cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                                                                                 <  1:1835848/100‑1 (MQ=255)
   ggggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg                                                                                                <  2:1370773/100‑1 (MQ=255)
   ggggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg                                                                                                <  2:1370706/100‑1 (MQ=255)
      gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                             <  1:1169971/100‑1 (MQ=255)
      gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                             <  1:2709598/100‑1 (MQ=255)
        tgttgtTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa                                                                                           <  1:1121989/100‑1 (MQ=255)
        tgttgtTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa                                                                                           <  1:1475562/100‑1 (MQ=255)
        tgttgtTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGgaga                                                                                           <  2:707150/100‑1 (MQ=255)
         gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                          >  2:2416745/1‑100 (MQ=255)
          ttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGata                                                                                         <  1:2291569/100‑1 (MQ=255)
           tgttCGTACCGGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat                                                                                        <  2:439436/100‑1 (MQ=255)
             ttCGTACCAGCGTCTTAGCGCCATTCCAGAGCCATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGGGTTGGGTCGTATCTCCCAGGGGGATTTGa                                                                                      >  2:2022257/1‑100 (MQ=255)
              tCGTGCCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAc                                                                                     <  2:2801777/100‑1 (MQ=255)
              tCGTACCAGCGTCTTAGCGCGAGTCCAGAGCAATTGGTGTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAc                                                                                     <  2:2801949/100‑1 (MQ=255)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                     <  1:2454675/100‑1 (MQ=39)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                     <  2:2731346/100‑1 (MQ=39)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                     <  1:3163028/100‑1 (MQ=39)
               cGTACCCGCGTCTCGGCGCGATTCCAGAGCATTTGGTTTCCAAGCGCCGAGGATTGCCACACGCGCAGTGTTGGGTCATAGCCCACTGGGTGATATGAcc                                                                                    <  2:2192693/100‑1 (MQ=255)
               cGTACCAGCTTCGTAGCGCCATTCCGGAGCGATTGGTTTCCAGGCACCCGGGATTGCCACACGCGTGTTGTTGGGTCATATCCCCCTGGTAGATATGAcc                                                                                    <  2:2656550/100‑1 (MQ=25)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAcc                                                                                    >  2:2825376/1‑100 (MQ=255)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCT‑CCAGGGGGATATGAccc                                                                                   <  2:460605/100‑1 (MQ=255)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                    <  1:1948500/100‑1 (MQ=38)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                    <  2:212476/100‑1 (MQ=38)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                    <  1:2795995/100‑1 (MQ=39)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                    <  2:1002477/100‑1 (MQ=38)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                    <  2:3090580/100‑1 (MQ=38)
                gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                   <  2:1924061/100‑1 (MQ=39)
                gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                   <  1:209173/100‑1 (MQ=38)
                gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTGTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                   <  2:2592325/100‑1 (MQ=38)
                 tACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGACCCa                                                                                  <  1:1024831/100‑1 (MQ=38)
                   ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt                                                                                >  1:2341080/1‑100 (MQ=255)
                   ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt                                                                                >  1:2384429/1‑100 (MQ=255)
                   ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGACCCaac                                                                                <  2:477231/100‑3 (MQ=38)
                    cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                               <  1:2105809/100‑1 (MQ=255)
                     aGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCAGGGGGATATGCCCCAGTTc                                                                              >  1:140367/1‑100 (MQ=255)
                          cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                         <  2:1441094/100‑1 (MQ=255)
                          cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                         <  1:229372/100‑1 (MQ=255)
                          cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                         <  1:1181894/100‑1 (MQ=255)
                          cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                         <  1:1940152/100‑1 (MQ=255)
                           ttAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGc                                                                        <  2:2884033/100‑1 (MQ=255)
                              gcgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCcac                                                                     <  2:1804285/100‑1 (MQ=255)
                              gcgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCcac                                                                     >  1:934753/1‑100 (MQ=255)
                              gcgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCcac                                                                     >  1:934151/1‑100 (MQ=255)
                                gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCacac                                                                   >  1:944413/1‑100 (MQ=255)
                                     tCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtgt                                                              <  2:2277358/100‑1 (MQ=255)
                                      ccAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                             <  2:2902282/100‑1 (MQ=255)
                                        agagCAATTGGTTTCCATGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGCCGCTAGGACCCAGTTCGCCTGCCACACCGTGTGct                                                           >  2:2187162/1‑99 (MQ=255)
                                        agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCa                                                           <  2:1797287/100‑1 (MQ=255)
                                          agCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc                                                         <  1:628208/100‑1 (MQ=255)
                                            ccaTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGTCCCAGTTCGCCTGCCACACCGTGTGCACCCg                                                       <  1:99563/98‑1 (MQ=255)
                                            cAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCg                                                       <  1:1984249/100‑1 (MQ=255)
                                            cAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCg                                                       <  1:2704515/100‑1 (MQ=255)
                                              aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTc                                                     >  1:1334374/1‑100 (MQ=255)
                                                 ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                  <  2:33934/100‑1 (MQ=255)
                                                 ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                  >  1:839252/1‑100 (MQ=255)
                                                    ttCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgc                                               <  2:2972686/100‑1 (MQ=255)
                                                     tCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgcg                                              >  2:815419/1‑100 (MQ=255)
                                                     tCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgcg                                              >  1:536907/1‑100 (MQ=255)
                                                       cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCa                                            >  1:1827680/1‑100 (MQ=255)
                                                         ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                          >  2:2699976/1‑100 (MQ=255)
                                                         ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                          >  2:2701034/1‑100 (MQ=255)
                                                         ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                          <  2:1131459/100‑1 (MQ=255)
                                                            aCCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGtt                                       <  1:1681115/100‑1 (MQ=255)
                                                              ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                                     <  2:1980399/100‑1 (MQ=255)
                                                                 ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATcc                                  <  2:2179423/100‑1 (MQ=255)
                                                                 ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATcc                                  <  1:2250395/100‑1 (MQ=255)
                                                                 ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGAATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATcc                                  <  1:1703140/100‑1 (MQ=255)
                                                                   aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCcac                                <  2:2288453/100‑1 (MQ=255)
                                                                        cacaCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                           >  1:2501376/1‑100 (MQ=255)
                                                                        cacaCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                           >  1:2501735/1‑100 (MQ=255)
                                                                        cacaCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                           >  1:2501312/1‑100 (MQ=255)
                                                                           acGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACCCTGCgca                        >  2:2261814/1‑98 (MQ=255)
                                                                             gcgcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcc                      <  2:2476345/100‑1 (MQ=255)
                                                                             gcgcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcc                      <  1:212466/100‑1 (MQ=255)
                                                                                 cgaactGGGTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAt                  >  2:1207477/6‑100 (MQ=37)
                                                                                  gtgtTGGGTCATATCTCCCAGGGGGATAGGACCCAGTTCGCCTGCCACACCTTTTGCACCCGACCACGGCGCACCGGTCATCCACACTGCGAACCCCATc                 <  2:3031228/100‑1 (MQ=255)
                                                                                     ttGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcccc              <  2:1498944/100‑1 (MQ=255)
                                                                                      tGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg             <  2:2374843/100‑1 (MQ=255)
                                                                                      tGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg             >  1:1256450/1‑100 (MQ=255)
                                                                                      tGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg             <  1:2279806/100‑1 (MQ=255)
                                                                                      tGGGTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg             >  1:3142094/1‑100 (MQ=38)
                                                                                       gggTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGCTCATCCACACTGCGAACCCCATCCCCGt            >  2:2967417/1‑100 (MQ=255)
                                                                                       gggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt            >  1:647685/1‑100 (MQ=38)
                                                                                       gggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt            >  2:1208204/1‑100 (MQ=38)
                                                                                       gggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCAAACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt            >  1:2622824/1‑100 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa           >  2:1461079/1‑100 (MQ=38)
                                                                                         gTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc          >  1:118679/1‑100 (MQ=255)
                                                                                          tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc         <  2:1573079/100‑1 (MQ=255)
                                                                                          tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc         <  1:2142083/100‑1 (MQ=255)
                                                                                          tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc         >  2:1273632/1‑100 (MQ=255)
                                                                                           natatCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGGCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg        >  1:1644075/2‑100 (MQ=255)
                                                                                           cATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg        >  2:3139266/1‑100 (MQ=255)
                                                                                             tatCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag      >  1:919645/1‑100 (MQ=255)
                                                                                              atCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgaga     <  1:718030/100‑1 (MQ=255)
                                                                                               cctcCCCGGGGGATATGACCCAGTTCGCCTGCCACACAGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGAt    <  2:2082494/99‑1 (MQ=255)
                                                                                                 tcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAg  >  2:2569042/1‑100 (MQ=255)
                                                                                                 tcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAg  <  1:1095138/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
TTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAG  >  NC_000913/4307193‑4307389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑