Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,999 T→G 11.3% L217V (TTG→GTG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,9990TG11.3% 1586.5 / 68.8 603L217V (TTG→GTG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (182/339);  new base G (79/3);  total (261/342)
Fisher's exact test for biased strand distribution p-value = 1.80e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 638 total aligned reads displayed.

ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148
                                                                                                                                                     |                                                                                                                                                     
  atTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTttg                                                                                                                                                     >  1:5236611/1‑150 (MQ=255)
   ttCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTttgt                                                                                                                                                    <  2:6060324/150‑1 (MQ=255)
     ccTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGGTTGAGTCATTATTTGTCCCGCGGTCGTttgttg                                                                                                                                                  >  1:1313106/1‑150 (MQ=255)
       tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTGTGTtgtg                                                                                                                                                >  1:3748406/1‑150 (MQ=255)
        gCCGGAATGCAGTGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTgg                                                                                                                                               <  1:7883359/150‑1 (MQ=255)
        gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTgg                                                                                                                                               <  2:3089931/150‑1 (MQ=255)
         ccGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGc                                                                                                                                              >  2:7916768/1‑150 (MQ=255)
         ccGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGc                                                                                                                                              >  2:259365/1‑150 (MQ=255)
          cGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCt                                                                                                                                             <  1:8248311/150‑1 (MQ=255)
            gAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGa                                                                                                                                           <  1:1096125/150‑1 (MQ=255)
            gAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTGGCTGa                                                                                                                                           >  1:1668211/1‑150 (MQ=255)
                   gagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGtt                                                                                                                                    <  1:5687161/150‑1 (MQ=255)
                     gCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGa                                                                                                                                  >  1:1478862/1‑150 (MQ=255)
                           aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTg                                                                                                                            <  2:1186916/150‑1 (MQ=255)
                           aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTg                                                                                                                            <  1:448548/150‑1 (MQ=255)
                              aaaaaaGGTTGCTGCTGGCAACAGCGGTTTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGAGGTCGTTTGTTGTGTCTGACGCCGTTGATTTCTTCCa                                                                                                                         >  2:8223240/3‑150 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATc                                                                                                                       <  1:3313921/150‑1 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATGTCTGCCCTc                                                                                                                       >  1:7579637/1‑150 (MQ=255)
                                  aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTCTGTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGTTTTCTGGCCTCgg                                                                                                                     >  2:2856609/1‑150 (MQ=255)
                                  aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCgg                                                                                                                     <  1:4971414/150‑1 (MQ=255)
                                  aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCgg                                                                                                                     <  1:705905/150‑1 (MQ=255)
                                  aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCGGACGCCGTTGATTTCTGCCATCgg                                                                                                                     >  1:1391373/1‑150 (MQ=255)
                                     gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCCTTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgt                                                                                                                  >  2:7121719/1‑150 (MQ=255)
                                     gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGGTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTTATTTCTGCCATCGGCgt                                                                                                                  >  1:7298018/1‑150 (MQ=255)
                                     gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTCTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACAGTTTGAGTCATTATTTGTCCCGCGGTCGTTTTTTGTGGCTTACGCCGTTGATTTCTGCCAACGGCgt                                                                                                                  >  2:4627169/1‑150 (MQ=255)
                                       tgctgcTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtgg                                                                                                                <  2:7053669/150‑1 (MQ=255)
                                       tgctgcTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGGCGTTTGTTGTGGCTGACGCCGTTGATTTATGCCATCGGCgtgg                                                                                                                >  1:6929352/1‑150 (MQ=255)
                                        gctgctGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTGTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGATTTCTGCCCTCGGCgtggt                                                                                                               >  1:7353663/1‑150 (MQ=255)
                                           gctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                                                                                                            <  1:2708514/150‑1 (MQ=255)
                                              ggCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCt                                                                                                         <  2:433110/150‑1 (MQ=255)
                                                cAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATGATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtgg                                                                                                       >  2:3510838/1‑150 (MQ=255)
                                                  aCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtg                                                                                                     <  2:4436191/150‑1 (MQ=255)
                                                   cAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtgg                                                                                                    <  1:6295468/150‑1 (MQ=255)
                                                   cAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtgg                                                                                                    <  1:4599830/150‑1 (MQ=255)
                                                     gCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATTGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTTTCCCGCGGTCGTGTGTTGTGGCTGGCGCCCTTGATTTATGTCAACCGCGTGGTTGCGCATgtggtg                                                                                                  >  1:5844934/1‑150 (MQ=255)
                                                      cGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGgtgt                                                                                                 <  1:7953535/150‑1 (MQ=255)
                                                       ggTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGtt                                                                                                >  2:6349832/1‑150 (MQ=255)
                                                       ggTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGtt                                                                                                <  1:4852808/150‑1 (MQ=255)
                                                        gtgtTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTTTTGCAGAAGCCGTTGATTTCTGCCAACCCCGTGGTGGCGCAGGTGGGGTTa                                                                                               >  1:2253340/1‑150 (MQ=255)
                                                        gtgtTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTTGCTGACGCCGTTGATTTCTGCCATCTGCGTGGTGGCGCTGGTGGTGTTa                                                                                               >  1:8080658/1‑150 (MQ=255)
                                                           tttttGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTTGCTGACGCCGTTGATTTATTCCATCGGCGTGGTGGCGCTGGTGGTGTTAAtt                                                                                            >  2:1495812/1‑150 (MQ=255)
                                                           tttttGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAAtt                                                                                            <  2:3810935/150‑1 (MQ=255)
                                                              ttGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGc                                                                                         <  1:6761533/150‑1 (MQ=255)
                                                                gtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCa                                                                                       <  2:1406251/150‑1 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                    <  1:6442304/150‑1 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                    <  1:7222263/150‑1 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                    <  2:3981267/150‑1 (MQ=255)
                                                                    gTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCg                                                                                   >  2:4774853/1‑150 (MQ=255)
                                                                     ttGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtgg                                                                                                       >  2:2039964/1‑129 (MQ=255)
                                                                      tggtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGtt                                                                                 <  2:4036884/150‑1 (MQ=255)
                                                                        gtggCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGa                                                                               <  1:4752521/150‑1 (MQ=255)
                                                                            ctggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGcc                                                                           <  1:3002627/150‑1 (MQ=255)
                                                                            ctggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGcc                                                                           <  2:6718035/150‑1 (MQ=255)
                                                                             tggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTTGCTGACGCCGGTGATTTATTCCCACGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCCCCCCTTGATGCCg                                                                          >  1:5193273/1‑150 (MQ=255)
                                                                             tggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                                                          <  1:4346954/150‑1 (MQ=255)
                                                                              ggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCgg                                                                         <  2:2941297/150‑1 (MQ=255)
                                                                               gctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgt                                                                        <  1:7483632/150‑1 (MQ=255)
                                                                               gctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgt                                                                        <  1:3862020/150‑1 (MQ=255)
                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCGGACGCCCGTGATTTCAGCCCGCCGCCTGGTGGCGCGGGTGGTGTTAATTACCCCCCCCATGATGcccgggg                                                                      >  1:1604530/1‑145 (MQ=255)
                                                                                   tgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtat                                                                    >  2:4538172/1‑150 (MQ=255)
                                                                                     cTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                  >  2:5812302/1‑150 (MQ=255)
                                                                                      tGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATc                                                                 <  2:3406605/150‑1 (MQ=255)
                                                                                      tGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGGTGTGGCTGACGCCGGTGATTTATTCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCGTGTTGCCGGTGTATATc                                                                 >  1:6056036/1‑150 (MQ=255)
                                                                                        gCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTCTTCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATAGa                                                               >  2:8056453/1‑150 (MQ=255)
                                                                                        gCGGTTATGGCGCAGCGGTAAAGCGACTGAACGTTTTAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTTTTAATTCGCCACCCGTTGATGCCGGTGTATATCGa                                                               <  1:6163064/150‑1 (MQ=255)
                                                                                             tATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAAATCGCCACCCGTTGATGCCGGTGTATATAGATAttt                                                          >  2:369246/1‑150 (MQ=255)
                                                                                                ggCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGc                                                       <  2:3188201/150‑1 (MQ=255)
                                                                                                 gcgcAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCg                                                      <  1:5360981/150‑1 (MQ=255)
                                                                                                   gcAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTTGCGGGCGCCGTTGGTTTATGGCATCGGCGTGGGGGCGCTGGGGGTGTTTATTGGCCACCCGGTGTTTGCGGTGTATATGGATAtttttggtt                                                    >  1:86646/1‑145 (MQ=255)
                                                                                                     aGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaaa                                                  <  1:3444080/150‑1 (MQ=255)
                                                                                                        ggTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTg                                               <  2:192942/150‑1 (MQ=255)
                                                                                                         gTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTgg                                              <  1:2856609/150‑1 (MQ=255)
                                                                                                           aaaGCGACTGAGCGTTTGATTAATTATTTGTCCCGCTGTCGTTTGTTGTGGCTGACGCAGTTGATTTCTGCCATCGGCGTGGTGGAGCTGGTGGTTTTAATTCGCCACCCGTTGATTCCGGTGTATATCGATATTTTGCGTAAAGTGGtt                                            <  1:6634969/150‑1 (MQ=255)
                                                                                                             aGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTgg                                          <  2:2388726/150‑1 (MQ=255)
                                                                                                             aGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTgg                                          <  2:7559889/150‑1 (MQ=255)
                                                                                                               cGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTgggg                                        <  1:1006712/150‑1 (MQ=255)
                                                                                                               cGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTgggg                                        <  1:2048825/150‑1 (MQ=255)
                                                                                                                  cTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGttt                                     <  2:6237669/150‑1 (MQ=255)
                                                                                                                    gACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAg                                   <  2:6592741/150‑1 (MQ=255)
                                                                                                                        gTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTTCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCCTTGATGCCGGTGTATATAGATATTTTGCGTAAAGTGGTTGGGGTTTAGgaga                               >  2:1682246/1‑150 (MQ=255)
                                                                                                                          ttGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATa                             <  1:3991498/150‑1 (MQ=255)
                                                                                                                          ttGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATa                             <  2:3609587/150‑1 (MQ=255)
                                                                                                                               tCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGGTTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCtt                        <  2:5427637/150‑1 (MQ=255)
                                                                                                                                 attattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTtgt                      >  2:5272753/1‑150 (MQ=255)
                                                                                                                                  ttattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTtgtg                     >  2:8440747/1‑150 (MQ=255)
                                                                                                                                  ttattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTtgtg                     >  2:5545260/1‑150 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCgg                  <  1:962314/150‑1 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTAGTTTCTGACGCCGGTGATTTATTACATACGCGTTGTTGCCCTTGTTGTGTTAATTAGCCACCCCTTTATTCCGGTGTATAAACATATTTTTCGTAAAGTGGTTTGGGTTTAGGAGATATTCTTTTTCgg                  >  2:5675454/1‑150 (MQ=255)
                                                                                                                                      ttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCggg                 <  2:7352655/150‑1 (MQ=255)
                                                                                                                                      ttGTCCCGCGGGCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCggg                 <  1:5462497/150‑1 (MQ=255)
                                                                                                                                       tGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGt                <  2:1043559/150‑1 (MQ=255)
                                                                                                                                        gTCCCGCGGTCGTTTTTTGTGGCTGACGCCCTTGATTTAAGCCATCGGCGTGGTGGCGCTGGTTGTGTTAAATAGCCCCCCCTTGATTCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTATTAGATATTCTTGTTCGGGtt               >  2:2234841/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGGTCGTTTGTTGTGGCTTACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCCTTGATTCCGGTGTATAAAGATATTTTGCGTAAAGTGGTTGGGGTTTATGAGATAGTATTTTGCGGGttt              >  2:158123/1‑149 (MQ=255)
                                                                                                                                          cccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGc             <  2:495939/150‑1 (MQ=255)
                                                                                                                                            cgcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCt           <  1:2459203/150‑1 (MQ=255)
                                                                                                                                              cgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGa         <  2:2266381/150‑1 (MQ=255)
                                                                                                                                               ggTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGAt        <  2:2674745/150‑1 (MQ=255)
                                                                                                                                                   gTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGa    <  2:6450180/150‑1 (MQ=255)
                                                                                                                                                    tttGTTGTGGCTGACGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGAAGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGAc   >  1:6517810/1‑150 (MQ=255)
                                                                                                                                                     ttgttgTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACg  <  2:7749615/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: