Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,248 G→A 5.1% intergenic (‑507/+151) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,2480GA 5.1% 1504.1 / 47.6 510intergenic (‑507/+151)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base G (309/173);  new base A (14/12);  total (323/187)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 516 total aligned reads displayed.

TTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACA  >  NC_000913/4326099‑4326397
                                                                                                                                                     |                                                                                                                                                     
tttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCAGCGGAGGGTGCGGGACTGTGGAGTCCAGATAAGACGACCAAGCGTCGAAACAGGCAGGGGGCGCCg                                                                                                                                                       >  2:1201183/1‑150 (MQ=11)
        ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGAAGGCACCGTTGCCGGa                                                                                                                                               >  2:6630564/1‑150 (MQ=255)
         cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGAt                                                                                                                                              >  2:3206925/1‑150 (MQ=37)
          tGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATg                                                                                                                                             >  1:6355770/1‑150 (MQ=37)
           gATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgc                                                                                                                                            >  2:1785417/1‑150 (MQ=37)
            aTAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcg                                                                                                                                           <  1:1502343/150‑1 (MQ=17)
             tAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCcggcggcgg                                                                                                                                          >  1:3157441/1‑150 (MQ=255)
              aaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggc                                                                                                                                         <  2:8144251/150‑1 (MQ=17)
                gACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTATGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGt                                                                                                                                       >  1:3108128/1‑150 (MQ=37)
                gACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGt                                                                                                                                       >  2:5026886/1‑150 (MQ=37)
                    cgcAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAAc                                                                                                                                   >  1:3939116/1‑150 (MQ=37)
                      cAAGCGTCGCATCAGGCAGAAGGCACTTTTGCCGGATGCGCCGTAAACCCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTACGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGc                                                                                                                                 <  1:3517278/150‑1 (MQ=11)
                       aaGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                >  2:6203128/1‑150 (MQ=35)
                           gTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGCATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                            >  1:8149501/1‑150 (MQ=21)
                            tCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                           >  1:4600769/1‑150 (MQ=34)
                            tCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGAAGGCACCGTTGCCGGATGCGGCGAGATCGCCTTATc                                                                                                                           >  2:2261763/1‑150 (MQ=11)
                               cATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                        >  2:4258603/1‑150 (MQ=34)
                                aTCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                       <  1:7391440/150‑1 (MQ=34)
                                    ggCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                   <  1:5120750/150‑1 (MQ=34)
                                        gTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCCGACGGCACCGTTGCCGGATGCGGCGGACACGCCGGATCCGGCCGACGGAg                                                                                                               >  2:7897665/1‑150 (MQ=11)
                                         ttGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAATCGCCTTATCCGGCCTACGGAgg                                                                                                              >  2:6060818/1‑150 (MQ=18)
                                         ttGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg                                                                                                              >  2:3672718/1‑150 (MQ=33)
                                            gCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                           >  2:5847744/1‑150 (MQ=32)
                                              aCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCTACGGAGGGTGCg                                                                                                         >  2:8342547/1‑150 (MQ=17)
                                              aCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg                                                                                                         <  1:3277370/150‑1 (MQ=32)
                                               ccattGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg                                                                                                        <  1:5223663/147‑1 (MQ=1)
                                                  ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGaa                                                                                                     <  1:1679507/150‑1 (MQ=32)
                                                     ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAttt                                                                                                  <  1:4396237/150‑1 (MQ=32)
                                                      cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGGGCGGGACTTTg                                                                                                 >  2:4123413/1‑150 (MQ=12)
                                                      cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTg                                                                                                 >  1:3745625/1‑150 (MQ=32)
                                                       ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGt                                                                                                <  2:4506311/150‑1 (MQ=32)
                                                            cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGCGTGCGGCCAGGTGTAGGcc                                                                                           >  1:6441775/1‑150 (MQ=11)
                                                             ggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                          <  1:499646/150‑1 (MQ=32)
                                                             ggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCGACGGAGGGTGCGGGCAGTTGTAGGCCt                                                                                          >  2:5181050/1‑150 (MQ=255)
                                                              gcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTg                                                                                         >  1:5370872/1‑150 (MQ=32)
                                                              gcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTGGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGAGGGGGCGGGACGGTGTAGGCCTg                                                                                         >  2:7698413/1‑150 (MQ=11)
                                                               cgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGa                                                                                        <  2:3476947/150‑1 (MQ=32)
                                                                gTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGAt                                                                                       <  2:6934000/150‑1 (MQ=32)
                                                                   aaCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTGAGCGCCGTATCCGGCCTACGGAGGGGGCGGGCATTGGTTGGGATGATAAg                                                                                    >  1:2226018/1‑150 (MQ=11)
                                                                     cGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGACTTTGTAGGCCTGATAAGAc                                                                                  >  1:6694565/1‑150 (MQ=17)
                                                                     cGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAc                                                                                  >  1:972001/1‑150 (MQ=32)
                                                                      gCCTTATCCGGCCTCCGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc                                                                                            <  2:1370322/139‑1 (MQ=17)
                                                                      gCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAcg                                                                                 >  1:3450772/1‑150 (MQ=32)
                                                                         ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACgcgc                                                                              >  2:7276564/1‑150 (MQ=32)
                                                                            tCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAg                                                                           >  1:7032511/1‑150 (MQ=32)
                                                                              cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                         >  1:6039859/1‑150 (MQ=32)
                                                                              cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                         >  2:3094126/1‑150 (MQ=32)
                                                                              cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACGGAGGGTGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCg                                                                         >  2:394115/1‑150 (MQ=17)
                                                                              cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTAGGCACCGTTGCCGGATGCGGCGAAAGCGCCTTATCCGTACTGCCGAGCGTCGGGGACGGAGTCGGCCTTATAAGACGCGCacgag                                                                         >  2:2752446/1‑146 (MQ=11)
                                                                                 ccTACGGAGGGTGCTGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCg                                                                      <  2:5311452/150‑1 (MQ=17)
                                                                                             gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCt                                                          >  2:6757593/1‑150 (MQ=34)
                                                                                              cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTg                                                         >  2:6450785/1‑150 (MQ=35)
                                                                                              cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTg                                                         >  2:7505505/1‑150 (MQ=25)
                                                                                              cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCATATACGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACTg                                                         >  1:1101/1‑150 (MQ=12)
                                                                                               gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                       <  1:2777135/135‑1 (MQ=32)
                                                                                               gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg                                                                <  2:4835734/142‑1 (MQ=32)
                                                                                               gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTgg                                                        >  2:8003669/1‑150 (MQ=35)
                                                                                               gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTgg                                                        >  1:7574154/1‑150 (MQ=35)
                                                                                                ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCCTCTGGc                                                       >  1:6947858/1‑150 (MQ=25)
                                                                                                 gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg                                                                                                         <  1:492276/99‑1 (MQ=33)
                                                                                                 gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCa                                                      <  1:5583467/150‑1 (MQ=37)
                                                                                                 gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCa                                                      >  1:8420528/1‑150 (MQ=17)
                                                                                                  aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                          <  2:2595129/113‑1 (MQ=32)
                                                                                                   acgagTAGGCCTGAAAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCAcc                                                    <  1:7888026/146‑1 (MQ=255)
                                                                                                           gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCgg                                            >  2:2112471/1‑150 (MQ=39)
                                                                                                           gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCgg                                            >  2:6988526/1‑150 (MQ=39)
                                                                                                           gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCgg                                            <  1:2026219/150‑1 (MQ=39)
                                                                                                           gCCAGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCgg                                            >  2:443941/1‑150 (MQ=255)
                                                                                                            ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGa                                           >  2:6309260/1‑150 (MQ=255)
                                                                                                             cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                          >  1:4459691/1‑102 (MQ=32)
                                                                                                               gATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAcgc                                                                                <  1:4220635/110‑1 (MQ=32)
                                                                                                                acaaGACGCGCCAGCGTCGCATCAGGCAGTCGGCACAGTGGCCTGATGCGGCGTACACGGCTTTTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATgcg                                       <  1:1201183/148‑1 (MQ=14)
                                                                                                                aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGa                                                                                   <  2:7141455/106‑1 (MQ=32)
                                                                                                                    gACGCGCACGCGTCGCATCAGGCAGTCGGCACCGGGGCCGGATGCGGCGTAAACGCCTCATCCGGCCTACGGAGGGAGCGGGAATTTGGAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGgcgc                                   <  2:5989749/150‑1 (MQ=14)
                                                                                                                    gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATACGGCCGACGGAGGGTGCGGGGATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGAAGGCATCTGGCACCGATGCCGGATGCGgcgc                                   >  1:6125607/1‑150 (MQ=14)
                                                                                                                    gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCATATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGGTGCCGGATGCGgcgc                                   >  1:627060/1‑150 (MQ=255)
                                                                                                                     aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTAATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGcc                                              <  2:6864180/138‑1 (MQ=21)
                                                                                                                       gcgcCAGCGTCGCATCAGGCAGACGGCACCGTTGCCGGCTGCTGCTTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAg                                <  1:5445421/150‑1 (MQ=14)
                                                                                                                       gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGaa                                                                                                     >  2:4719956/1‑81 (MQ=33)
                                                                                                                       gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGaa                                                                                                     <  1:4719956/81‑1 (MQ=33)
                                                                                                                       gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCATATCCGGCCGACGGAGGGTGGGGGGATTTGTTGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAACAGGCACCCTTTCCGGATGCGGCGCGAg                                >  1:7242182/1‑150 (MQ=11)
                                                                                                                        cgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAgc                               <  2:3809571/150‑1 (MQ=255)
                                                                                                                        cgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAgc                               >  2:2835982/1‑150 (MQ=255)
                                                                                                                          ccaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGTACACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATACGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAgcgc                             <  1:7251972/148‑1 (MQ=255)
                                                                                                                          cacgCGTCGCATCAGGCAGTCGGCTCCGTTGCCGTTTGCGTCGTAAACGCCTTATCCGGGCTACGGATGGTGCGGGTTCTTTTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAgcgc                             <  2:7385751/147‑1 (MQ=14)
                                                                                                                          cAAGCGTCGCATCAGGCAGCCGGCACCGTTTCCGGATTGGGCGACTTGTGCTCATCCGGGCTCCGGTGGGAGCTGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAgcgc                             <  1:2593883/150‑1 (MQ=14)
                                                                                                                                  gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCg                     <  1:5847744/150‑1 (MQ=255)
                                                                                                                                   cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCgg                    >  2:4587514/1‑150 (MQ=255)
                                                                                                                                   cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCgg                    >  2:5570517/1‑150 (MQ=255)
                                                                                                                                    aTCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                   >  1:4038262/1‑150 (MQ=255)
                                                                                                                                     tCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTTCGGTTGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCt                  <  1:7962476/150‑1 (MQ=255)
                                                                                                                                     tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGcc                                              >  2:406025/1‑122 (MQ=25)
                                                                                                                                         gCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTAcc              <  1:2924653/150‑1 (MQ=255)
                                                                                                                                         gCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTAcc              >  2:3022826/1‑150 (MQ=255)
                                                                                                                                                gCACCGTTGCCGGATGCGGCGTAACGGCCTTATCCGGGCTTCTGAGGGTGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATcaca       <  1:7739445/150‑1 (MQ=17)
                                                                                                                                                gCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATcaca       <  2:8224227/150‑1 (MQ=255)
                                                                                                                                                gCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATcaca       <  1:4100202/150‑1 (MQ=255)
                                                                                                                                                 cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCACAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCacaa      <  2:1651914/150‑1 (MQ=255)
                                                                                                                                                 cACCGTTGCCGGAAGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCacaa      <  1:8227450/150‑1 (MQ=255)
                                                                                                                                                    cGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTACCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCacaatac   <  1:3852093/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACA  >  NC_000913/4326099‑4326397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: