Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 784,979 G→T 6.8% I113I (ATC→ATA ybgS ← putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,9790GT 6.8% 1588.4 / 12.0 482I113I (ATC→ATAybgSputative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (174/258);  new base T (48/2);  total (222/260)
Fisher's exact test for biased strand distribution p-value = 1.45e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAT  >  NC_000913/784830‑785127
                                                                                                                                                     |                                                                                                                                                     
  aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  2:5994128/150‑1 (MQ=255)
  aTCCCTTAAGTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  1:6472291/150‑1 (MQ=255)
     ccTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCAGTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACCTCCTGATTGATGcc                                                                                                                                                 >  1:4120295/1‑150 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCa                                                                                                                                                >  2:1040058/1‑150 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCCCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCa                                                                                                                                                <  1:6375738/150‑1 (MQ=255)
       tttattattCTCACTTAACTTTATGACATAATACTGCTTTTAGTTGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGTCTATTTTGCAGGGTTTCTGCGTGGTACCTTCTGTTTTGTTATCGACTTCATTATTGATGCCAt                                                                                                                                               <  1:5217110/147‑1 (MQ=255)
              ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGt                                                                                                                                        <  2:2938202/150‑1 (MQ=255)
              ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGt                                                                                                                                        >  1:6323202/1‑150 (MQ=255)
                 tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTTATGCCATCCCCGGTTTg                                                                                                                                     >  2:1667173/1‑150 (MQ=255)
                 tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTg                                                                                                                                     >  1:5618325/1‑150 (MQ=255)
                 tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTg                                                                                                                                     >  1:5500719/1‑150 (MQ=255)
                  cACTTAACTTTATGACACCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGt                                                                                                                                    <  2:5385973/150‑1 (MQ=255)
                    cTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTAc                                                                                                                                  >  2:2081493/1‑150 (MQ=255)
                    cTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCAATATTGATGCCCACAACCGGttttac                                                                                                                                   >  2:2281356/1‑147 (MQ=255)
                     ttAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATTCCCACACCCGGTTGTAcc                                                                                                                                 >  2:2437714/1‑150 (MQ=255)
                        aCTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCttt                                                                                                                              <  1:8203294/150‑1 (MQ=255)
                         cTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCtttt                                                                                                                             <  1:1110832/150‑1 (MQ=255)
                          tttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAGCAATATTGATGCCATCACCGGGTTGTACCttttt                                                                                                                            >  1:5792257/1‑150 (MQ=255)
                           ttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTa                                                                                                                           <  1:4267269/150‑1 (MQ=255)
                               gACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCATCACCGGTTTGTACCTTTTTAttta                                                                                                                       >  1:7330476/1‑148 (MQ=255)
                                aCATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGat                                                                                                                      <  2:2632983/150‑1 (MQ=255)
                                aCATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCCGTTTGTACCTTTTTATTGat                                                                                                                      >  2:3472469/1‑150 (MQ=255)
                                     atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCCTTATTTATGCCATCACCGGGTTGTACCTTTTTATTGATATCCg                                                                                                                 >  1:1011770/1‑150 (MQ=255)
                                             tttAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGa                                                                                                         <  1:7972162/150‑1 (MQ=255)
                                              ttAGAAGTTAAAAATTAAAAGGGTGAGTCTCCGCTCTCCCATTTTTGGCTTTTTTGCTGGGTTACTGGGTGGTACCGTCGTTTTTGTTATCGAAATAATTTTTTTTGCAATCACCGGTTTGTACCTTTTTTTTTATATCCGGACAGCGAc                                                                                                        <  1:3454519/150‑1 (MQ=255)
                                              ttAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc                                                                                                        <  2:2386168/150‑1 (MQ=255)
                                                 taaGTTAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAt                                                                                                     <  1:2428529/149‑1 (MQ=255)
                                                 gAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAt                                                                                                     <  1:4773481/150‑1 (MQ=255)
                                                 gAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATAATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAt                                                                                                     <  1:3265895/150‑1 (MQ=255)
                                                       aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCCTCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCCACCATAGTTGc                                                                                               >  2:7683710/1‑150 (MQ=255)
                                                       aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTAGCGTCCACATTAAGGAGTCCATCACCGGTGTGTACCATTTTATTGATATCCGGACAGCGACCAACTTTGc                                                                                               >  2:654347/1‑150 (MQ=255)
                                                       aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGGTACTGCGTGGTCCCGTCGGTTTTGGTATCGACAACCATATTGATGCCATCACCCGTTTGTACCGTTTTATTGAAATCCGGACAGccaccaacgatgc                                                                                               >  2:1748360/1‑143 (MQ=255)
                                                        aaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCATTATTGATGCCATCACCGGGTTGTACCATTTTATTGATATACGGACAGCGACCATCTTTGca                                                                                              >  2:197434/1‑150 (MQ=255)
                                                           aTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACAt                                                                                           <  2:4125078/150‑1 (MQ=255)
                                                            ttAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCCTTATTGATGCCATCACCGGGTTGTACCTTTTTATTTATATCCGGACAGCGACCAACTTTTCACATg                                                                                          >  2:7816776/1‑150 (MQ=255)
                                                                aaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATTCCATAACCGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCATCTTTGCACATGgtgt                                                                                      >  2:3563095/1‑150 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGGTTGTACCTGTTTATTGTTATCCGGACAGCGACCATCTTTGCACATGGGGTTCt                                                                                   >  1:1715759/1‑150 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAACATTATTGATTCCATCACCCGTTTGTAACTTTTTATTTATATACGGACAGCGACCAAATTTTCAAATTGTGTTCt                                                                                   >  2:7923334/1‑150 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTTGTATCGACATCATTATTTATGCCATCACCGGTTTGTACCTTTTTATTGTTATCCGGACCTCGACCATCTTTGCACCTGGTGTTCt                                                                                   >  1:806426/1‑150 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCAATATTTATTCCAACACCCGTTTGTAACATTTTATTGATATCCGGAAACCGACCATCTTTGCACATGGTGTTCtt                                                                                  >  2:1426502/1‑150 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                  <  1:3921915/150‑1 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                  <  2:5056541/150‑1 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                  <  2:6800731/150‑1 (MQ=255)
                                                                      gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCAACACCGGTTTGTACCTTTTTATTTATATACGGACAGCGACCATATTTTCACATGGTGTTCTtgt                                                                                >  1:6842959/1‑150 (MQ=255)
                                                                      gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgt                                                                                <  1:7816776/150‑1 (MQ=255)
                                                                      gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgt                                                                                <  2:4836086/150‑1 (MQ=255)
                                                                      gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgt                                                                                <  2:821373/150‑1 (MQ=255)
                                                                      gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACAGAATTATTTATGCCAACACCCGGTTGTACCTTTTTATTTATATACGGACAGCGACCATCTTTTCACAAGGTTTTCtttt                                                                                >  1:2044957/1‑148 (MQ=255)
                                                                       tgaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgtg                                                                               <  1:6104974/149‑1 (MQ=255)
                                                                        gaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGc                                                                              <  1:1539419/150‑1 (MQ=255)
                                                                                tctcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTGATTCCATAACCCGTTTGTACCATTTTATTGATATCAGGACAACGACCAACTTTTCAAATTGTGTTCTTGTGCTATTCAta                                                                      >  1:6057983/1‑149 (MQ=255)
                                                                                  tcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAACACCGGTTTGTACCTTTTTATTGATATACGGACAGCGACCATCTTTGCACATGGTGTTCTTGTTCTCTTCATCtt                                                                    >  2:5867877/1‑150 (MQ=255)
                                                                                   cccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCATTATTTATTCCATCACCCGTTTGTACCATTTTATTTATATCCGGACAGCGACCAACTTTGCACATGGTGTTCTTGTGCTCTTCAtaatt                                                                   >  2:2722633/1‑146 (MQ=255)
                                                                                    ccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTg                                                                  <  1:2242033/150‑1 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACAACATTATTTATGCCATCACCCGTTTGTAACATTTTATTTATATACGGACAGCGACCATAATTTCAAAAGGTGTTATTTTTCTCTTAATAttttg                                                                 >  1:5293544/1‑148 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTAAAGAAATAATTATTTATTCCATCACCGGTTTGTAACATTTTATTTATAAACGGAAAGCGACCAAATTTTCACAAGGTGTTATTGTTCTATTCATAttttg                                                                 >  2:3115153/1‑148 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCAACACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATATTTgg                                                                 >  2:6933329/1‑150 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGGATAGACATCAATATTGATGCCATCACCCGTTTGTACCATTTTATTTATATCCGGACAGCGACCAACTTTGCACAAGGTGTTGTTTTGCTCTTAATCttttg                                                                 >  1:28533/1‑148 (MQ=255)
                                                                                               tATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCATTATTTTTGCCAACACCCGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCATTGCACAAGGTGTTATTTTTCTCTTAATAATTGGTCATTACGTc                                                       >  1:4505558/1‑150 (MQ=255)
                                                                                                 ttttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAt                                                     <  2:1283984/150‑1 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                    <  1:4024901/150‑1 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                    <  2:464607/150‑1 (MQ=255)
                                                                                                     gCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCttt                                                                                                                              >  2:8354198/1‑73 (MQ=255)
                                                                                                       aGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCa                                               >  2:6123169/1‑150 (MQ=255)
                                                                                                           ttACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTga                                           >  2:3821305/1‑150 (MQ=255)
                                                                                                            tACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTgaa                                          <  1:7755396/150‑1 (MQ=255)
                                                                                                                gCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAAc                                      <  2:7131229/150‑1 (MQ=255)
                                                                                                                      tACCGTCGGTTTTGGTATCGACAACATTATTTATTCCATCACCGGTTTGTAACTTTTTATTGATATACGGACACCGACCATATTTGCACAAGGTGTTATTTTTCTCTTCATCTTTTGTCATTACGTAATTTTTCATTGAATAAACAtaca                                >  2:4462451/1‑147 (MQ=255)
                                                                                                                        ccGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGaa                              <  1:5716069/150‑1 (MQ=255)
                                                                                                                          gTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGCGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCACCCGAATg                            >  2:1067867/1‑150 (MQ=255)
                                                                                                                            cGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgca                          >  2:7420918/1‑150 (MQ=255)
                                                                                                                                 ttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAtt                     <  2:6323202/150‑1 (MQ=255)
                                                                                                                                    gTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATtgtg                  <  2:857663/150‑1 (MQ=255)
                                                                                                                                    gTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATtgtg                  >  1:8289632/1‑150 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgc                 >  1:1943299/1‑150 (MQ=255)
                                                                                                                                      aTCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgcc                <  2:4600942/150‑1 (MQ=255)
                                                                                                                                       tCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccg               >  2:4257913/1‑150 (MQ=255)
                                                                                                                                       tCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccg               <  1:3801513/150‑1 (MQ=255)
                                                                                                                                         gACTTCATGAGTGATGCCATCACCGGTGTGTACCTTTTTCTTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc             <  1:2104045/150‑1 (MQ=255)
                                                                                                                                         gACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc             <  1:1272724/150‑1 (MQ=255)
                                                                                                                                          aCATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCa            <  2:5293416/150‑1 (MQ=255)
                                                                                                                                          aCATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCAGTCCGTCATTGTTCATTGAAGAACCATCCGACTGCAGCATTGTGCCGCCa            <  2:3637849/150‑1 (MQ=255)
                                                                                                                                            atcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGa          >  1:3534186/1‑150 (MQ=255)
                                                                                                                                            atcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGa          >  2:8279847/1‑150 (MQ=255)
                                                                                                                                            atcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGa          >  2:5661634/1‑150 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa         <  1:7721534/150‑1 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa         <  2:6707507/150‑1 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa         >  1:7556090/1‑150 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa         <  1:6718857/150‑1 (MQ=255)
                                                                                                                                             tcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGaa         <  2:8091499/150‑1 (MQ=255)
                                                                                                                                               attattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgc              >  2:1913260/1‑143 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg      <  1:461818/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg      >  1:3862708/1‑150 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg      <  1:5889024/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg      <  1:2661624/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg      >  2:3901928/1‑150 (MQ=255)
                                                                                                                                                 tattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCgg     <  1:708353/150‑1 (MQ=255)
                                                                                                                                                  attGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCACCCGAATGCAGCATTGTGCCGCCAGAACCGGt    <  1:1335042/150‑1 (MQ=255)
                                                                                                                                                   ttGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa   <  2:2044957/150‑1 (MQ=255)
                                                                                                                                                   ttGATGCCATCACCGGTTTGGACCTTTTTATTGAGATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa   <  1:4951008/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAT  >  NC_000913/784830‑785127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: