Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,226 T→G 7.5% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2260TG 7.5% 613.3 / 4.4 269intergenic (‑369/+126)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (167/43);  new base G (10/17);  total (201/68)
Fisher's exact test for biased strand distribution p-value = 1.12e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGG  >  NC_000913/915077‑915373
                                                                                                                                                     |                                                                                                                                                    
 aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                     >  1:1020945/1‑150 (MQ=32)
   ggATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                                  >  2:357754/1‑150 (MQ=32)
       gtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCAGGTAGGAGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGATCGCCAGAGCCCGGTGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCt                                                                                                                                              >  2:5446335/1‑150 (MQ=11)
          ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                           >  2:8426385/1‑150 (MQ=32)
          ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCt                                                                                                                                           >  2:2980462/1‑150 (MQ=32)
          ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTcca                                                                                                                                           >  1:6532399/1‑149 (MQ=17)
             tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTgc                                                                                                                                        >  1:1520934/1‑150 (MQ=17)
                  gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                   >  2:1410824/1‑150 (MQ=32)
                   cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGt                                                                                                                                  >  1:1793503/1‑150 (MQ=32)
                    gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTg                                                                                                                                 >  1:1324308/1‑150 (MQ=32)
                    gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTg                                                                                                                                 >  2:4804328/1‑150 (MQ=32)
                      gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                               >  1:5562753/1‑150 (MQ=33)
                      gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCAGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGc                                                                                                                               >  1:5003978/1‑150 (MQ=18)
                       ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCCGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGGGGCa                                                                                                                              >  1:3331110/1‑150 (MQ=18)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTACGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                        >  2:1860068/1‑150 (MQ=255)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                        >  1:4439096/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGt                                                                                                                        >  2:1836268/1‑150 (MQ=255)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                        >  2:7976906/1‑150 (MQ=255)
                               ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                      >  1:4603002/1‑150 (MQ=40)
                                 aTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATACGTCGAGGGCCCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCCGGGGCCCTATGTTGCt                                                                                                                    >  2:6154562/1‑150 (MQ=11)
                                 aTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                                                    >  2:1314206/1‑150 (MQ=255)
                                   gTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTa                                                                                                                  >  2:7251960/1‑150 (MQ=255)
                                   gTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTGCGCCCGTGGCAATATGTTGCTTa                                                                                                                  >  1:5406980/1‑150 (MQ=255)
                                       ccTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGGGGCAATATGTTGCTTAGCTc                                                                                                              >  1:3105282/1‑150 (MQ=255)
                                        cgcgcgCCCGACCGGGTCGCGTTATGGGATATGTGGTGGGCACCGCGCAGGGGGGTTTTTGTTCGCCAGCGCCGGGGGGTGCTGGGGGGGGCGGCTATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                             <  2:6993667/148‑1 (MQ=17)
                                        cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                             >  2:531149/1‑150 (MQ=255)
                                         tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGGGGCAATATGTTGCTTAGCTCAt                                                                                                            >  2:2066285/1‑150 (MQ=255)
                                            cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCATGCGCCACTGGCAATATGTTGCTTATCTCATGaa                                                                                                         >  1:6408017/1‑150 (MQ=255)
                                            cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCATGaa                                                                                                         >  1:8289896/1‑150 (MQ=255)
                                               ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGAGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCCCCAGTGGCAAAATGTTTCTTAGCACATGAAAgg                                                                                                      >  2:7824492/1‑150 (MQ=14)
                                                cGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGCCATTGGCCGCCCCCAGCGCGCGCCATGCGCCAGGTGCAAAATGTTGATTAGCTAAGGAAAggg                                                                                                     >  1:2214934/1‑149 (MQ=14)
                                                 gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCTGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                                                    >  2:6874073/1‑150 (MQ=255)
                                                 gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                                                    >  2:623739/1‑150 (MQ=255)
                                                 gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAggtg                                                                                                    >  1:8456610/1‑148 (MQ=255)
                                                  aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCACTGGCAATATGTTGCTTAGCTCATGAAAGGAgc                                                                                                   >  1:6759623/1‑150 (MQ=255)
                                                       gTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAAGATGTTGCTTAGCTCGTGGAAGGTGCGcaac                                                                                              >  1:4891352/1‑150 (MQ=17)
                                                            cGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCATGGGGCGGCGGCGATTTGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATTTTGCTTAGCTCATGTAAGGAGCGCAACAAgat                                                                                         >  1:5579793/1‑150 (MQ=255)
                                                              tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCAGCGCGCGCCATGCGCCCCGGGCAAAAAGTTTCTTAGCTCATTAAAGGAGCGcaacaataata                                                                                       >  1:7959164/1‑145 (MQ=17)
                                                              tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                                                       >  2:1637943/1‑150 (MQ=255)
                                                               aaGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatgat                                                                                      >  2:2931055/1‑150 (MQ=255)
                                                                aGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCAGCGCGCTCCATGCGCCAGGGGCAATAATTTTCTTAGCTCATGAAAGGTGCGCAAAAACattatg                                                                                     >  1:145586/1‑150 (MQ=17)
                                                                  ggATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCGTGCGCCCGGGGCAATATGTTGCGTAGCTGATGGACGGGGCGCAACCAGatgttgta                                                                                   >  1:1702931/1‑148 (MQ=17)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGCGCTTTGCCGCCCCCAGCGCGCGCCGTGCCCCAGTGGCAAAAATTTTCTTATCACATGAAAGGAGCGCAAAAAAATTATTaaa                                                                                  >  1:7291216/1‑149 (MQ=14)
                                                                         tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCGTGGCCGCCCCCCGCGCGCGCCATGCGCCAGGGGCAAAAAGTTGCTTAGCTCAAGGAATGAGCGCAACAAGAAGATGTATCaaaaa                                                                            >  1:110698/1‑147 (MQ=17)
                                                                           gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                                                          >  2:1677925/1‑150 (MQ=255)
                                                                               gCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaaca                                                                      >  2:1807083/1‑150 (MQ=255)
                                                                                 aCCGCGCAGCGGCGATTTTGTTAGCCAGAGCCCGGGGGTGCCCGGGGCGGCGGCGAGTGGCCGCCCCCGGCGCGCGCCATGCGCCCCGGGCCAAATTTTTATTATCTCAATAAAGGAGCGCAACAAAAAGATGAATCAACAAATaaaaac                                                                    >  1:4663630/1‑150 (MQ=9)
                                                                                   cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTTGCAATATGTTGCTTAGCTCAAGAAAGGAGCGCAACAAGATGATGAATAAAAATATAACAACAt                                                                  >  2:2141879/1‑150 (MQ=21)
                                                                                          ggtgttttttGTTCGCCGGCGCGGGGGGGTGGTGGGGGCGGGGGCGTTGGCCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaa                                                           <  1:622491/144‑1 (MQ=255)
                                                                                             cgATTTTGTTCGCCAGAGCCCGGGGGGGCTGGGGGGGGCGGCGTTTGGCCACCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                                                        <  2:4121177/150‑1 (MQ=25)
                                                                                                ttttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                                     <  2:7057257/150‑1 (MQ=255)
                                                                                                ttttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                                     >  2:1088585/1‑150 (MQ=255)
                                                                                                 tttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                                    >  2:5218845/1‑150 (MQ=255)
                                                                                                  tttttCCCCAGAGCCGGGGGGTGCGGGGGGGGGGGGGTATGGGCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                                                   <  2:418566/147‑1 (MQ=255)
                                                                                                     ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCGCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGAc                                                >  2:768475/1‑150 (MQ=255)
                                                                                                     ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCTTGCGCCAGTGGCAATATGTTGCATAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAACGCCTGAc                                                >  2:7360897/1‑150 (MQ=255)
                                                                                                         ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTa                                            >  2:339875/1‑150 (MQ=255)
                                                                                                          ccgcgCCGGGGGGTGCTGGGGGGGGGGGCGAGTGCCCCCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTAc                                           <  1:6966502/146‑1 (MQ=255)
                                                                                                           agagCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAGGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACg                                          >  2:4722596/1‑150 (MQ=255)
                                                                                                            gcgcgggggggTGGGGGGGGGGGGGGCTTTGGCCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGc                                         <  1:4062145/144‑1 (MQ=37)
                                                                                                             ggcgggggggTGGGGGGGGGGGGGGCTTTGGGCCCCCCCGCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGcc                                        <  2:6482546/145‑1 (MQ=255)
                                                                                                                  gggggTGCTGGGGGCGGCGGCTTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                   <  2:5517442/150‑1 (MQ=255)
                                                                                                                  gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                   >  2:159717/1‑150 (MQ=255)
                                                                                                                  gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                   >  2:3182415/1‑150 (MQ=255)
                                                                                                                  gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                   >  2:8376625/1‑150 (MQ=255)
                                                                                                                  gggggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                   >  2:2515177/1‑150 (MQ=255)
                                                                                                                   ggggggCTGGGGGCGGGGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGGCGTAATGTCAGGCCt                                  <  2:5406980/150‑1 (MQ=37)
                                                                                                                    gggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt                                 >  2:2368855/1‑150 (MQ=255)
                                                                                                                    gggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt                                 >  2:4220451/1‑150 (MQ=255)
                                                                                                                       tggggggggCGGCGCCGGTGGGCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                              <  1:3475455/146‑1 (MQ=255)
                                                                                                                        gggggggggggCGGGGCGGGGCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGc                             <  1:7190547/147‑1 (MQ=255)
                                                                                                                          tggggggggggGCGCTGGGCCGCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAGAAGGCCTGGCGTAATGTCAGGCCttttttta                           <  1:4739126/149‑7 (MQ=255)
                                                                                                                          aGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGt                           >  2:6362343/1‑150 (MQ=255)
                                                                                                                            ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                         >  2:3178812/1‑150 (MQ=255)
                                                                                                                             gggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                        >  2:2313078/1‑150 (MQ=255)
                                                                                                                              ggcggcggcGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaat                       >  1:4320260/1‑150 (MQ=255)
                                                                                                                                cggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatta                     >  2:4423468/1‑150 (MQ=255)
                                                                                                                                 ggcggcgTTTGGCCGCCCCCTGCGCGCTCCTAGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaattaa                    <  2:7632172/150‑1 (MQ=255)
                                                                                                                                  gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAACTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                   >  2:7544845/1‑150 (MQ=255)
                                                                                                                                  gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTGAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGGtaattaat                   >  2:1710615/1‑150 (MQ=255)
                                                                                                                                    gttttttGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTATCTCATGATAGTAGCGCAAAAAGATGATTAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                 <  1:600628/145‑1 (MQ=255)
                                                                                                                                    gggtatgggCCGCCCGCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                 <  1:590555/143‑1 (MQ=255)
                                                                                                                                    ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                 >  1:2508510/1‑150 (MQ=255)
                                                                                                                                    ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGGTAATTAATcc                 >  2:5828360/1‑149 (MQ=255)
                                                                                                                                     gcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCAc                >  1:667402/1‑150 (MQ=255)
                                                                                                                                     gcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCAc                >  2:3926644/1‑150 (MQ=255)
                                                                                                                                      cgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTCATTAATCACg               >  2:4821613/1‑150 (MQ=255)
                                                                                                                                      cgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg               <  2:5640843/150‑1 (MQ=255)
                                                                                                                                       gttgggcggcCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc              <  2:4439242/146‑1 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc              >  2:1818956/1‑150 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTGAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc              >  1:5216708/1‑150 (MQ=255)
                                                                                                                                        aTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAAAAACATCTTAAAAAAATGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCt             >  2:4895568/1‑150 (MQ=255)
                                                                                                                                           ggCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtttt          <  2:4663630/150‑1 (MQ=255)
                                                                                                                                            gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc         >  2:2627180/1‑150 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc        >  1:2926418/1‑150 (MQ=255)
                                                                                                                                              cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca       >  1:1908485/1‑150 (MQ=255)
                                                                                                                                              cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca       >  2:4273493/1‑150 (MQ=255)
                                                                                                                                              cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca       >  2:3701998/1‑150 (MQ=255)
                                                                                                                                                cccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc     >  2:6687745/1‑150 (MQ=255)
                                                                                                                                                cacccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCCGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc     >  2:6626767/3‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGG  >  NC_000913/915077‑915373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: