Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,932 C→T 5.1% intergenic (+30/‑29) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9320CT 5.1% 1793.6 / 51.7 525intergenic (+30/‑29)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base C (245/252);  new base T (21/6);  total (266/259)
Fisher's exact test for biased strand distribution p-value = 4.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 540 total aligned reads displayed.

TGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAG  >  NC_000913/1104784‑1105081
                                                                                                                                                    |                                                                                                                                                       
    ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                                    <  2:4212215/150‑1 (MQ=255)
    ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTCGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                                    <  1:6844566/150‑1 (MQ=255)
     gTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGccc                                                                                                                                                   >  1:4296131/1‑150 (MQ=255)
      ttAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCt                                                                                                                                                 >  1:6679424/1‑150 (MQ=255)
      ttAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCt                                                                                                                                                 >  1:8431170/1‑150 (MQ=255)
         aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                             >  2:3470497/1‑150 (MQ=255)
          aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                            >  2:5178038/1‑150 (MQ=255)
          aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                            >  2:2134224/1‑150 (MQ=255)
           cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtttt                                                                                                                                           <  2:7046978/150‑1 (MQ=255)
            aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACACGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttct                                                                                                                                          <  2:7408167/150‑3 (MQ=255)
              ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                        >  2:1320118/1‑150 (MQ=255)
               tCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtttttttt                                                                                                                                       <  1:3793631/150‑1 (MQ=255)
                cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTTGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                      <  2:6571301/150‑1 (MQ=255)
                cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                      >  2:1020855/1‑150 (MQ=255)
                cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                      <  1:2368112/150‑1 (MQ=255)
                 ggtggtGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCg                                                                                                                                     <  2:5790123/150‑1 (MQ=255)
                  gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                    <  2:8417514/150‑1 (MQ=255)
                  gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                    <  2:2299119/150‑1 (MQ=255)
                  gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                    <  1:583530/150‑1 (MQ=255)
                    ggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGga                                                                                                                                  <  1:562656/150‑1 (MQ=255)
                      tggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGgaga                                                                                                                                >  1:2691526/1‑150 (MQ=255)
                        gCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGagaag                                                                                                                              >  1:7195193/1‑150 (MQ=255)
                             ggTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAatat                                                                                                                         <  1:6371381/150‑1 (MQ=255)
                             ggTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAatat                                                                                                                         >  2:3266425/1‑150 (MQ=255)
                                gctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGaa                                                                                                                      <  1:465364/150‑1 (MQ=255)
                                 ctgcAGGTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                     <  2:2820622/150‑1 (MQ=255)
                                       ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACCGGGCGCAAGCCCTGATTTTTTTCGGGAGAAGAATATGAATACGtta                                                                                                               <  1:1635886/150‑1 (MQ=255)
                                       ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtta                                                                                                               >  2:7133402/1‑150 (MQ=255)
                                        tGACCAGACTGCATCTAACTCCTCTGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttat                                                                                                              <  1:3592104/150‑1 (MQ=255)
                                          aCCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttatta                                                                                                            >  1:7653855/1‑150 (MQ=255)
                                          aCCAGACTGCATCTAACGCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAAGACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGATTATGAATACGttatta                                                                                                            <  2:4794865/150‑1 (MQ=255)
                                            cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACt                                                                                                          <  1:8108816/150‑1 (MQ=255)
                                            cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACt                                                                                                          >  2:7315074/1‑150 (MQ=255)
                                                 tGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTg                                                                                                     >  1:7139549/1‑150 (MQ=255)
                                                  gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                    <  2:6688443/150‑1 (MQ=255)
                                                    aTCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                                  <  2:7923769/150‑1 (MQ=255)
                                                      cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                >  1:7079830/1‑150 (MQ=255)
                                                      cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                >  1:6132869/1‑150 (MQ=255)
                                                       tAACTCCTCCGTCAACTTTAATCATGTTTGCTTTGTTAAAAACGCGTACTCTAATCAGTACTTATACATCATTTTTATTACATAAACAGGGCGCAAGCCCTTTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                <  1:4732272/150‑1 (MQ=255)
                                                       tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                               <  2:4031341/150‑1 (MQ=255)
                                                       tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                               >  1:4985039/1‑150 (MQ=255)
                                                        aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACt                                                                                              >  2:3013639/1‑150 (MQ=255)
                                                        aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCACTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACt                                                                                              <  2:2599473/150‑1 (MQ=255)
                                                         aCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACtt                                                                                             >  2:7614812/1‑150 (MQ=255)
                                                             ctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCa                                                                                         >  1:7949145/1‑150 (MQ=255)
                                                             ctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCa                                                                                         <  1:2139057/150‑1 (MQ=255)
                                                                  tCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                    >  1:2544200/1‑150 (MQ=255)
                                                                       gTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAAc                                                                               <  2:5852239/150‑1 (MQ=255)
                                                                           cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                           <  1:548433/150‑1 (MQ=255)
                                                                             cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCATTACTAATACATAATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                         <  2:4489419/150‑1 (MQ=255)
                                                                             cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                         <  2:1182030/150‑1 (MQ=255)
                                                                             cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                         <  1:1020855/150‑1 (MQ=255)
                                                                               ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                       >  2:8234249/1‑150 (MQ=255)
                                                                                gTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATAc                                                                      >  1:3560446/1‑150 (MQ=255)
                                                                                gTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCATTGCGGCACTTTCCAGTCAGATAACCTTTAATaa                                                                      >  2:1647992/1‑149 (MQ=255)
                                                                                  tGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGa                                                                    <  2:1248861/150‑1 (MQ=255)
                                                                                   ggCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAc                                                                   <  2:2792430/150‑1 (MQ=255)
                                                                                       ttGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAg                                                               <  2:7810158/150‑1 (MQ=255)
                                                                                        tGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGc                                                              >  1:1291383/1‑150 (MQ=255)
                                                                                         ggTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                             >  2:5096003/1‑150 (MQ=255)
                                                                                         ggTAACAACGCGACCGCTCATAAGTACTAATACATCATTTGGATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                             >  2:4942502/1‑150 (MQ=255)
                                                                                          gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCaa                                                            <  2:621279/150‑1 (MQ=255)
                                                                                           tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTc                                                                                      <  2:1836542/123‑1 (MQ=255)
                                                                                           tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTc                                                                                      >  1:1836542/1‑123 (MQ=255)
                                                                                             acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                         >  2:4860679/1‑150 (MQ=255)
                                                                                                acGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAt                                                      <  1:5415912/150‑1 (MQ=255)
                                                                                                    gACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATgtgt                                                  <  1:1163761/150‑1 (MQ=255)
                                                                                                    gACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATgtgt                                                  <  1:4869412/150‑1 (MQ=255)
                                                                                                      ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                                <  1:2231064/150‑1 (MQ=255)
                                                                                                       cGCTCATCAGTACTAATACATCATTTGTATCACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtata                                               >  2:1975021/1‑150 (MQ=255)
                                                                                                       cGCTCATCAGTACTAATACATCATTTGTAGTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTACGGACAAGGATAAGAATAAGTTATTACTCCGTTCGGCACATTCCAGGACGATTACCGTTAATGCGCCCCAGCCAGGGGGTGTGtatt                                               >  2:549991/1‑149 (MQ=255)
                                                                                                          tcatcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCa                                            <  1:8282508/150‑1 (MQ=255)
                                                                                                             tcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatta                                         >  2:3115876/1‑150 (MQ=255)
                                                                                                                gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                      <  1:3407181/150‑1 (MQ=255)
                                                                                                                gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                      <  1:3266425/150‑1 (MQ=255)
                                                                                                                gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                      >  1:5070962/1‑150 (MQ=255)
                                                                                                                    tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                  >  2:1609776/1‑150 (MQ=255)
                                                                                                                    tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                  <  1:5220933/150‑1 (MQ=255)
                                                                                                                    tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                  >  2:6023878/1‑150 (MQ=255)
                                                                                                                     aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                                 <  2:3779877/150‑1 (MQ=255)
                                                                                                                        aCATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTc                              >  2:7470911/1‑150 (MQ=255)
                                                                                                                         catcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                             <  1:4455350/150‑1 (MQ=255)
                                                                                                                          atcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                             >  2:5285298/1‑150 (MQ=255)
                                                                                                                          atcatTTGTATTACAGAAACAGGGCGCAAGCCCAGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAc                            >  1:7355870/1‑150 (MQ=255)
                                                                                                                           tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAct                           <  1:5178039/150‑1 (MQ=255)
                                                                                                                            catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActc                          <  1:6584367/150‑1 (MQ=255)
                                                                                                                             atTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActct                         >  2:1408131/1‑150 (MQ=255)
                                                                                                                             atTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActct                         >  1:3951677/1‑150 (MQ=255)
                                                                                                                              tttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCtt                        >  1:5957777/1‑150 (MQ=255)
                                                                                                                                   aTTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                   <  2:6132869/150‑1 (MQ=255)
                                                                                                                                    gaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGGGTATACCATTATTCCTGAAGTCACTCTTACTCaa                  >  2:4313/7‑150 (MQ=255)
                                                                                                                                    gaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGGTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCaa                  >  2:4280949/7‑150 (MQ=255)
                                                                                                                                      aCAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc                <  2:6999580/150‑1 (MQ=255)
                                                                                                                                       cAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt               >  2:3694529/1‑150 (MQ=255)
                                                                                                                                        aGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCtt              >  2:8011532/1‑150 (MQ=255)
                                                                                                                                          aaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCCAGGGGCTGTGTATACCCTTATTCCTGAAGTCACTCTTACTCAAACTGGt            >  2:6884214/1‑150 (MQ=255)
                                                                                                                                            aCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt          <  1:3108654/150‑1 (MQ=255)
                                                                                                                                               gggCGCAAGCCCTGTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt        <  1:412741/150‑1 (MQ=255)
                                                                                                                                                ggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc      >  1:7447819/1‑150 (MQ=255)
                                                                                                                                                    cAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCagag  <  1:8234469/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAG  >  NC_000913/1104784‑1105081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: