Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,934 A→C 5.6% intergenic (+32/‑27) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9340AC 5.6% 1863.2 / 62.9 533intergenic (+32/‑27)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base A (243/257);  new base C (25/7);  total (268/265)
Fisher's exact test for biased strand distribution p-value = 1.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 538 total aligned reads displayed.

GACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTA  >  NC_000913/1104785‑1105083
                                                                                                                                                     |                                                                                                                                                      
  cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                      <  1:674852/150‑1 (MQ=255)
   ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                                     <  2:4212215/150‑1 (MQ=255)
   ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                                     <  2:7690385/150‑1 (MQ=255)
    gTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGccc                                                                                                                                                    <  1:4716586/150‑1 (MQ=255)
     ttAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCt                                                                                                                                                   <  2:7904286/150‑1 (MQ=255)
     ttAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAAACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCt                                                                                                                                                   >  2:8410109/1‑150 (MQ=255)
         aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                              >  2:2134224/1‑150 (MQ=255)
         aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                              >  2:5178038/1‑150 (MQ=255)
            gTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtttttt                                                                                                                                           <  2:6975184/150‑1 (MQ=255)
             ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                          >  1:2556548/1‑150 (MQ=255)
             ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                          >  2:5848124/1‑150 (MQ=255)
             ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                          >  1:4640582/1‑150 (MQ=255)
               cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                        <  2:8135700/150‑1 (MQ=255)
               cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                        <  1:2368143/150‑1 (MQ=255)
                ggtggtGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCg                                                                                                                                       <  2:5244078/150‑1 (MQ=255)
                 gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                      <  2:2299119/150‑1 (MQ=255)
                  tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtttttttttcgg                                                                                                                                     <  1:6582780/150‑5 (MQ=255)
                  tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                     >  1:2029629/1‑150 (MQ=255)
                   ggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGga                                                                                                                                    <  1:562656/150‑1 (MQ=255)
                     tggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGgaga                                                                                                                                  >  1:7931615/1‑150 (MQ=255)
                     tggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGgaga                                                                                                                                  <  2:4934019/150‑1 (MQ=255)
                      ggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGagaa                                                                                                                                 <  1:5478120/150‑1 (MQ=255)
                            ggTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAatat                                                                                                                           >  2:3266425/1‑150 (MQ=255)
                             gTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATg                                                                                                                          <  1:2254489/150‑1 (MQ=255)
                                ctgcAGGTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                       <  2:2820622/150‑1 (MQ=255)
                                 tgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATa                                                                                                                      >  2:4724995/1‑150 (MQ=255)
                                     gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                                  <  2:7452729/150‑1 (MQ=255)
                                      ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACCGGGCGCAAGCCCTGATTTTTTTCGGGAGAAGAATATGAATACGtta                                                                                                                 <  1:1635886/150‑1 (MQ=255)
                                      ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtta                                                                                                                 >  1:5216292/1‑150 (MQ=255)
                                       tGACCAGACTGCATCTAACTCCTCTGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttat                                                                                                                <  1:3592104/150‑1 (MQ=255)
                                           cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACt                                                                                                            >  1:6653893/1‑150 (MQ=255)
                                                  cATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCg                                                                                                     <  1:8097094/150‑1 (MQ=255)
                                                     cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                  >  1:6132869/1‑150 (MQ=255)
                                                     cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                  >  1:7079830/1‑150 (MQ=255)
                                                       aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACt                                                                                                >  2:3013639/1‑150 (MQ=255)
                                                       aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCACTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACt                                                                                                <  2:2599473/150‑1 (MQ=255)
                                                         ctcctcCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACttt                                                                                              >  2:3108654/1‑150 (MQ=255)
                                                          tcctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGGATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTc                                                                                             >  2:3465553/1‑150 (MQ=255)
                                                            ctccGTCAACGTGACTCAGGTTGGCTTTGGTACCAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAGACGTTATTACTCCTTGCGGCACTTTCCa                                                                                           <  2:5760740/150‑1 (MQ=255)
                                                              ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGt                                                                                         >  2:7131870/1‑150 (MQ=255)
                                                                           tCAGTTTGGCTTTGGTAACAACGCGACGGCTCATCATTACTAATACATCATTTGTATTACATAAACAGGGCGAATGCCATTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTa                                                                            <  1:943653/150‑1 (MQ=255)
                                                                           tCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTa                                                                            >  2:2778225/1‑150 (MQ=255)
                                                                           tCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTa                                                                            >  2:4021272/1‑150 (MQ=255)
                                                                            cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCATTACTAATACATAATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                           <  2:4489419/150‑1 (MQ=255)
                                                                            cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                           <  1:1020855/150‑1 (MQ=255)
                                                                               gTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCATTGCGGCACTTTCCAGTCAGATAACCTTTAATaa                                                                        >  2:1647992/1‑149 (MQ=255)
                                                                                     tttGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCa                                                                  >  2:1358655/1‑150 (MQ=255)
                                                                                     tttGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCa                                                                  >  1:3063972/1‑150 (MQ=255)
                                                                                       tGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGc                                                                >  1:1291383/1‑150 (MQ=255)
                                                                                         gTAACAACGCGACCTCTCATCAGTACTAATACATAATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAATAATATGAATACGTTATTACTCCTTGCTGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCaa                                                              <  2:8001557/150‑1 (MQ=255)
                                                                                          tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTc                                                                                        >  1:1836542/1‑123 (MQ=255)
                                                                                          tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                             <  2:5095325/150‑1 (MQ=255)
                                                                                          tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                             >  1:7329468/1‑150 (MQ=255)
                                                                                            acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           >  2:4860679/1‑150 (MQ=255)
                                                                                            acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           >  2:1239925/1‑150 (MQ=255)
                                                                                            acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           >  2:7598427/1‑150 (MQ=255)
                                                                                            acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           <  2:7368676/150‑1 (MQ=255)
                                                                                            acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           >  2:1835984/1‑150 (MQ=255)
                                                                                              aacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa                                                         >  2:1152561/1‑150 (MQ=255)
                                                                                              aacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCAAGCAAGGGGa                                                         >  2:1309975/1‑150 (MQ=255)
                                                                                               acGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAt                                                        >  2:6545981/1‑150 (MQ=255)
                                                                                               acGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAt                                                        <  1:5415912/150‑1 (MQ=255)
                                                                                                   gACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATgtgt                                                    <  1:1163761/150‑1 (MQ=255)
                                                                                                   gACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATgtgt                                                    <  1:4869412/150‑1 (MQ=255)
                                                                                                     ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                                  <  2:4302098/150‑1 (MQ=255)
                                                                                                      cGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtata                                                 >  1:4965778/1‑150 (MQ=255)
                                                                                                      cGCTCATCAGTACTAATACATCATTTGTATCACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtata                                                 >  2:1975021/1‑150 (MQ=255)
                                                                                                      cGCTCATCAGTACTAATACATCATTTGTAGTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTACGGACAAGGATAAGAATAAGTTATTACTCCGTTCGGCACATTCCAGGACGATTACCGTTAATGCGCCCCAGCCAGGGGGTGTGtatt                                                 >  2:549991/1‑149 (MQ=255)
                                                                                                         tcatcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCa                                              <  1:8282482/150‑1 (MQ=255)
                                                                                                           atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatt                                            <  1:5821413/150‑1 (MQ=255)
                                                                                                               gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                        <  1:3266425/150‑1 (MQ=255)
                                                                                                               gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                        <  1:3407181/150‑1 (MQ=255)
                                                                                                               gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                        >  1:5070962/1‑150 (MQ=255)
                                                                                                                 aCTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCt                                      <  1:7183823/150‑1 (MQ=255)
                                                                                                                  cTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTg                                     <  1:425475/150‑1 (MQ=255)
                                                                                                                   tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                    >  2:6023878/1‑150 (MQ=255)
                                                                                                                   tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                    >  2:1609776/1‑150 (MQ=255)
                                                                                                                    aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                                   <  2:3779877/150‑1 (MQ=255)
                                                                                                                    aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                                   <  1:7133402/150‑1 (MQ=255)
                                                                                                                        catcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                               <  1:4455350/150‑1 (MQ=255)
                                                                                                                         atcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATAAGACCCAGCAAGGGGATGTGTATACCATTATTACTGAAGTCAc                              >  2:4271175/1‑150 (MQ=255)
                                                                                                                          tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAct                             <  1:5178038/150‑1 (MQ=255)
                                                                                                                           catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActc                            <  1:3558630/150‑1 (MQ=255)
                                                                                                                           catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActc                            <  2:6193915/150‑1 (MQ=255)
                                                                                                                            atTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActct                           >  1:3951677/1‑150 (MQ=255)
                                                                                                                             tttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCtt                          >  1:5957586/1‑150 (MQ=255)
                                                                                                                              ttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTa                         <  2:729421/150‑1 (MQ=255)
                                                                                                                              ttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTa                         <  1:8410109/150‑1 (MQ=255)
                                                                                                                                   gaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCaa                    >  2:6128063/7‑150 (MQ=255)
                                                                                                                                      cAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt                 >  2:3694529/1‑150 (MQ=255)
                                                                                                                                      aaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt                 >  1:7434744/4‑150 (MQ=255)
                                                                                                                                      aaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCATGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt                 >  1:3758912/4‑150 (MQ=255)
                                                                                                                                         aaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCCAGGGGCTGTGTATACCCTTATTCCTGAAGTCACTCTTACTCAAACTGGt              >  2:6884214/1‑150 (MQ=255)
                                                                                                                                         aaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGt              >  1:7627663/1‑150 (MQ=255)
                                                                                                                                             aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt          >  2:6660792/1‑150 (MQ=255)
                                                                                                                                             aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt          >  2:7046024/1‑150 (MQ=255)
                                                                                                                                              gggCGCAAGCCCTGTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt          <  1:412741/150‑1 (MQ=255)
                                                                                                                                                gcgcAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCa       >  1:6327673/1‑150 (MQ=255)
                                                                                                                                                   cAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCagag    <  1:8234249/150‑1 (MQ=255)
                                                                                                                                                     aGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTa  <  1:2703717/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTA  >  NC_000913/1104785‑1105083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: