Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,945 G→C 5.4% intergenic (+419/+121) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9450GC 5.4% 981.4 / 40.0 311intergenic (+419/+121)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base G (123/167);  new base C (14/2);  total (141/170)
Fisher's exact test for biased strand distribution p-value = 4.44e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

Only 100 of 313 total aligned reads displayed.

TCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCG  >  NC_000913/1286796‑1287092
                                                                                                                                                     |                                                                                                                                                   
tCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTg                                                                                                                                                     >  1:4822716/1‑150 (MQ=255)
  gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTTCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCg                                                                                                                                                   >  2:884902/1‑150 (MQ=255)
  gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCg                                                                                                                                                   <  1:936616/150‑1 (MQ=255)
   caCCTGGGTCGAAGCTTCTCAGCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAGGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                  <  2:2320863/149‑1 (MQ=14)
   aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                  <  2:3497847/150‑1 (MQ=255)
   aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                  <  1:6741240/150‑1 (MQ=255)
   aaCCTTGGTCGAAGCTTCGCATCCTTCCCCGCGTTGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                  <  1:3340185/150‑1 (MQ=255)
      cTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                                                                                                                                               <  1:2681217/150‑1 (MQ=255)
      cTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                                                                                                                                               <  1:5746000/150‑1 (MQ=255)
      cTTGGACGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATAATTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                                                                                                                                               <  1:7855190/150‑1 (MQ=255)
        tGGTCGAAGGTTCTGATCGGTCCCCGCTTGGGCAGATTTTTTTATTGCGGAGTAGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAACg                                                                                                                                             <  2:5897339/150‑1 (MQ=14)
         ggTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGc                                                                                                                                            <  1:3782879/150‑1 (MQ=255)
                 cTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc                                                                                                                                    >  2:8263217/1‑150 (MQ=255)
                   tctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg                                                                                                                                  >  1:1397956/1‑150 (MQ=255)
                   tctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg                                                                                                                                  >  1:3531908/1‑150 (MQ=255)
                    atcATCCGTCCCCGCTTGGGCAGATTATTTGATTGCGGATTCGTTAGAGAATTCCGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                 <  2:4259268/149‑1 (MQ=255)
                     tcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCCCACGCTgtc                                                                                                                                >  1:49238/1‑148 (MQ=14)
                       aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                              >  1:4195534/1‑150 (MQ=255)
                       aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                              >  1:5565590/1‑150 (MQ=255)
                         ccgtCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTc                                                                                                                            <  1:5220376/147‑1 (MQ=255)
                         ccTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCAATCACGCtctcgcac                                                                                                                            >  1:7949192/1‑143 (MQ=14)
                           ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCGCACGCTGACGCTCGa                                                                                                                          >  1:72637/1‑150 (MQ=255)
                           ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTGGTGAAAGAGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCAGCGGCACCGCGCGCTCGCGGAcgcgcgc                                                                                                                          >  2:7851035/1‑149 (MQ=14)
                            tCCCCGCTGGGGCAGAATATTTGAGTGCGGAGTCGTTTGAGAATTCCGGGGCGTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                         <  1:5805518/150‑1 (MQ=14)
                              cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                       <  2:6687926/150‑1 (MQ=255)
                                cGCTTGGGCAGAATCTATGATTGCGGATTCGTTTGAGAATTCCGGGGATTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGa                                                                                                                     <  1:1274086/150‑1 (MQ=255)
                                cGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGa                                                                                                                     <  2:3955266/150‑1 (MQ=255)
                                  cTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCATGCGGCAACGCACTCTCGCTGTCGCTCGAGACGAAc                                                                                                                   >  1:8241647/1‑150 (MQ=255)
                                        ctgAATTTTTGCGTGCGGTTTGGTTTGAGTATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                             <  1:1846464/148‑1 (MQ=14)
                                        cAGACTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACTCGCAGCCGCTCGAGACGAACCTTCGt                                                                                                             >  2:2697092/1‑150 (MQ=14)
                                         aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                            >  2:645400/1‑150 (MQ=255)
                                         aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACAAACATCTGCGCCCTTCTCCCAACGGGACGATTCAGACCGCAACGCACTCAAGCGGACCCCCGAGCCCCACCGTAGTc                                                                                                            >  1:3231031/1‑150 (MQ=9)
                                           aaTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACACGCTGACGCTCAAGCCGTAAAATAGTCGa                                                                                                          >  2:2778581/1‑150 (MQ=14)
                                             tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCATAGTCGAAg                                                                                                        >  2:4357996/1‑150 (MQ=255)
                                               tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCACACGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                      >  2:3022781/1‑150 (MQ=255)
                                                    tttcGGATTCGTTTGAGTATTCCGTGTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                 <  2:5113406/147‑1 (MQ=14)
                                                    ttGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                 <  1:2562904/150‑1 (MQ=255)
                                                      gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                               <  1:4002688/150‑1 (MQ=255)
                                                       cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                              <  1:4449259/150‑1 (MQ=255)
                                                       cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                              <  1:6987427/150‑1 (MQ=255)
                                                               tttGAGTATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                      <  2:6232081/150‑1 (MQ=255)
                                                               tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCATCCCCGc                                                                                      >  2:1832484/1‑150 (MQ=255)
                                                               tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTATTAGAAGCATCTCAACCATcccccc                                                                                      >  1:1591945/1‑148 (MQ=14)
                                                                 tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                    <  2:6326890/150‑1 (MQ=255)
                                                                 tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                    <  2:2997423/150‑1 (MQ=255)
                                                                 tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                    >  2:6904480/1‑150 (MQ=255)
                                                                  gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACAAACAGCTGCGCCCTTCGCCCATCTGTTCGTTGCATGAGGCACAGCGCTCTCGCGGACGCGCGATCCGGACCTAGGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                   >  2:6137880/1‑150 (MQ=14)
                                                                   agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                  <  1:7661675/150‑1 (MQ=255)
                                                                     aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCACTCACGCAGACGCTCCAGCCCAACCATAGTCGAAGCATCTCAACCATACCCCCCTGGGc                                                                                >  1:5643512/1‑150 (MQ=14)
                                                                           ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                          <  1:708588/150‑1 (MQ=39)
                                                                            gggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                         >  2:5369314/1‑150 (MQ=255)
                                                                             ggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                        <  1:6177288/150‑1 (MQ=38)
                                                                              gCTTTGGAAAGTGCTGGTGGTGGGTGATGGATTACTCAGCGCTGCGAGCTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGCTGTCTCTCCGACGCTCTAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATAttt                                                                       <  2:5710628/150‑1 (MQ=11)
                                                                                ttttGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                     <  2:8324990/150‑1 (MQ=38)
                                                                                ttttGATAGTTATGGTGGTGGGGGATGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                     <  1:1519627/150‑1 (MQ=255)
                                                                                ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCATCGGCAACCCTCTAACCCAGACGCACGAGCCCAACAATAGTCGAAGCATATCAAAAATCACCCCAAGGGCAGAATATTTGa                                                                     >  1:4353062/1‑150 (MQ=2)
                                                                                  ttGTAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAtt                                                                   <  1:2222154/150‑1 (MQ=255)
                                                                                   tGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCAGCGCGCATCGCCCTACGGGTCGTAGCCTGCGGCAACGCTCTCTACCACGCGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTGATTg                                                                  >  2:4667481/1‑150 (MQ=11)
                                                                                      aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                               <  1:1958710/150‑1 (MQ=39)
                                                                                       aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGCGCCGACCCTTAGTCGAAGCGGCTCATCCGTCCCCGCCGGGGCCGAATATTTGTTTGCggg                                                              >  2:253695/1‑149 (MQ=14)
                                                                                          gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                           >  2:6739482/1‑150 (MQ=21)
                                                                                          gATGGTGGTGGGGGAAGGACTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTc                                                           <  2:7188566/150‑1 (MQ=255)
                                                                                           aTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTGATTGctattctt                                                          >  2:1966362/1‑143 (MQ=255)
                                                                                            tggtggtggtggAAGGATTTCTCAGCGCTGCGCTCTTCGCCCTTCGGGCCGTTGGCTGCTGCTAAGCTCTCTCGCTGTCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                         <  2:1528303/150‑1 (MQ=11)
                                                                                            tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                         <  2:2477969/150‑1 (MQ=40)
                                                                                              gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                       <  2:1870151/150‑1 (MQ=255)
                                                                                                 gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                    <  2:316267/150‑1 (MQ=255)
                                                                                                  tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                   <  1:719341/150‑1 (MQ=255)
                                                                                                  tggGGGAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                   <  1:6568634/150‑1 (MQ=255)
                                                                                                       gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                              <  2:6199489/150‑1 (MQ=2)
                                                                                                       gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                              <  1:2736958/150‑1 (MQ=255)
                                                                                                       gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                              <  2:6131515/150‑1 (MQ=255)
                                                                                                        aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                             <  1:5710878/150‑1 (MQ=255)
                                                                                                        aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTACCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                             <  1:8400648/150‑1 (MQ=255)
                                                                                                         aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                            <  2:5018954/150‑1 (MQ=255)
                                                                                                           gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                          <  1:4138679/150‑1 (MQ=255)
                                                                                                                cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                     <  1:5985167/150‑1 (MQ=255)
                                                                                                                 tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTg                                    >  2:3292299/1‑150 (MQ=255)
                                                                                                                 tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTATCGCGGACGCGCGACCCGAACCATAGTCGAAGCGTATCAACCGTCCCCGCATGGGGAGAAGATGTGAGTGCGGAGTCGCGTGAGAGGGAAGGGACTGTTg                                    >  2:7067121/1‑150 (MQ=9)
                                                                                                                  cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGa                                   <  2:5787817/150‑1 (MQ=255)
                                                                                                                   aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGACGCGCGAGCCGAACCGTAGTCGAAGCTTCTCAACCGTACCCCCAGGGGCAGAAAATTTGATTGCGGATTCGCGTGGGAGTTCAGGGACGTTTgta                                  >  2:2152617/1‑148 (MQ=14)
                                                                                                                       cggcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                              <  2:2646982/148‑1 (MQ=255)
                                                                                                                        tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                       >  2:5298619/1‑60 (MQ=40)
                                                                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTGATG                           >  2:7657382‑M1/1‑150 (MQ=255)
                                                                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATG                           <  2:4822716‑M1/150‑1 (MQ=255)
                                                                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATG                           <  2:3411042‑M1/150‑1 (MQ=255)
                                                                                                                               ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGT                      <  1:5458592‑M1/150‑1 (MQ=255)
                                                                                                                                     cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                <  2:5565590‑M1/150‑1 (MQ=255)
                                                                                                                                       tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG              <  1:2987034‑M1/150‑1 (MQ=255)
                                                                                                                                        gagagcgttgcCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAAAGTGATGTTGGTGGGGGAAGG             >  2:8125435‑M1/6‑150 (MQ=255)
                                                                                                                                        cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG             <  1:790257‑M1/150‑1 (MQ=255)
                                                                                                                                         agagcgttgCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGA            >  1:8068774‑M1/5‑150 (MQ=255)
                                                                                                                                           gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT          >  2:2009458‑M1/1‑150 (MQ=255)
                                                                                                                                           agcgtTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGGGAGTTCAGGGACTGTTGAAAGTGATGGTGGGGGGGGAAGGATa          >  2:8190741‑M1/3‑149 (MQ=255)
                                                                                                                                            tCGTTGCCTGCGGCACCGCACTCCCGCTGTCTCTCGAGCCGAACCTTAGTCGACGCTTCTCATCGCTCCCCGGATGGGCAGACTTTTTGATTGGGGCTTCGATTGAGCGTTGTTTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa         <  2:751113/150‑1 (MQ=14)
                                                                                                                                            gcgTTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGGTCGTTCAGGGACGTGTGAAAGTGATGGTGGTGGGGGAAGGATTc         >  2:8024691‑M1/2‑149 (MQ=255)
                                                                                                                                            gcGTTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa         >  2:3832491/2‑150 (MQ=255)
                                                                                                                                             cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTATAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCTCTTGATAGTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAc        <  1:2402636/150‑1 (MQ=17)
                                                                                                                                               ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTATTGAAAGTGATGGTGGTGGGGGAAGGATTACTc      >  2:4414017/1‑150 (MQ=255)
                                                                                                                                                 gCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAg    <  1:3054085/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCG  >  NC_000913/1286796‑1287092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: