BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	5.7%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	5.7%	1305.8 / 31.2	388	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (214/150); new base A (9/15); total (223/165)
Fisher's exact test for biased strand distribution p-value = 5.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGA  >  NC_000913/2817900‑2818197

actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCAGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                       >  1:636285/1‑150 (MQ=255)

  taAAGCCATATCAATTTAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     <  1:5038397/150‑1 (MQ=255)

  taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     >  2:5074990/1‑150 (MQ=255)

  taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGATAACATCCCTAGTGCCTGCGGTTAATTCAGCTACCACTTCGATACCTACATTGCTGAAGATTTCg                                                                                                                                                     >  2:1501109/1‑150 (MQ=17)

    aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCGAAAGCTACGTTATCCTCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   <  2:2700126/150‑1 (MQ=255)

     aGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATAATCCCTAGTGCCTGCGGTTAATACAGCTACCTCTTCGATACCTACATTCATGAAGATTTCGgag                                                                                                                                                  >  1:370135/1‑150 (MQ=17)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                >  1:3403957/1‑150 (MQ=255)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                <  2:696148/150‑1 (MQ=255)

          tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                             >  1:6611225/1‑150 (MQ=255)

          tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGAGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                             >  1:2394400/1‑150 (MQ=255)

            tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                           >  2:6392361/1‑150 (MQ=255)

              aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                                                                                                         >  1:7054668/1‑150 (MQ=255)

                ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                       >  2:3019008/1‑150 (MQ=255)

                  aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAAGATGGTGCATc                                                                                                                                     >  1:3287808/1‑150 (MQ=255)

                    gagaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCAGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCg                                                                                                                                   >  2:4608985/1‑150 (MQ=255)

                     agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                                  <  2:6237413/150‑1 (MQ=255)

                     agaAGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                                  >  2:5248792/1‑150 (MQ=255)

                      gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                                 >  2:2494576/1‑150 (MQ=255)

                        tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggag                                                                                                                               >  1:6232946/1‑150 (MQ=255)

                          gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                             >  1:1544844/1‑150 (MQ=255)

                          gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAAGATGGTGCATCCGggagga                                                                                                                             >  2:4729210/1‑150 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                           <  1:103510/150‑1 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                           >  1:723695/1‑150 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                           <  1:5539335/150‑1 (MQ=255)

                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         >  2:3359393/1‑150 (MQ=255)

                               tCCGGGAGGATTACTCGCCTTGCGGCTCGCGCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCAGCTACCTCTTCGATACCTTCATTGCAGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGa                                                                                                                        >  1:7606283/1‑150 (MQ=255)

                                     aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTcc                                                                                                                 <  1:2880120/150‑1 (MQ=255)

                                      ggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCg                                                                                                                <  2:5824766/150‑1 (MQ=255)

                                         ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGG‑TTCGAATcctcccgga                                                                                                             <  2:2618697/150‑5 (MQ=18)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGCGAAGCTGGTGCATCCGGGAGGAGTCGCA‑CCTCCGACCGcgc                                                                                                         >  1:4596772/1‑148 (MQ=18)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                         >  2:3421519/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                         >  1:5864879/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                         >  2:7117885/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                         >  2:2560051/1‑150 (MQ=255)

                                                 cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGtt                                                                                                     >  2:2067555/1‑150 (MQ=255)

                                                  ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTc                                                                                                    <  1:2132436/150‑1 (MQ=255)

                                                    gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGt                                                                                                  <  1:4690224/150‑1 (MQ=255)

                                                    gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGt                                                                                                  >  2:5924312/1‑150 (MQ=255)

                                                       gCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGc                                                                                               >  2:8046876/1‑150 (MQ=255)

                                                        cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAATCCGCTACCTCATCGATACCATCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGcc                                                                                              >  1:4369805/1‑150 (MQ=255)

                                                           gCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAg                                                                                           >  1:594964/1‑150 (MQ=255)

                                                             ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                         <  1:5382957/150‑1 (MQ=255)

                                                             ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                         <  2:3864336/150‑1 (MQ=255)

                                                             ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                         >  1:4781866/1‑150 (MQ=255)

                                                               ttCGGGCCGTTGCTTAAGCAACGTTATCATCCCTTGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                                                                       <  1:7909034/150‑1 (MQ=255)

                                                               ttCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                                                                       >  2:5989869/1‑150 (MQ=255)

                                                                       gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                               <  1:5275995/150‑1 (MQ=255)

                                                                       gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                               >  2:4315715/1‑150 (MQ=255)

                                                                         tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                             >  1:5171861/1‑150 (MQ=255)

                                                                           cTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                           >  1:8271200/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                          >  2:5404710/1‑150 (MQ=255)

                                                                               aGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                       >  2:2262762/1‑150 (MQ=255)

                                                                                 cAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                     >  1:2311097/1‑150 (MQ=255)

                                                                                        atcatcCCTAGTGCCTGAGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATTCAt                                                              <  2:3614855/150‑1 (MQ=255)

                                                                                           atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                           >  2:771627/1‑150 (MQ=255)

                                                                                             cccTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGa                                                         <  2:4931134/150‑1 (MQ=255)

                                                                                               ctggTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                       >  2:4023882/4‑150 (MQ=17)

                                                                                                tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGATCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGGAAat                                                      >  2:6140197/1‑150 (MQ=255)

                                                                                                     ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTGTATCCAGCTGAGCTACGGATGCATGGGGTtatttatt                                                 >  1:124457/1‑150 (MQ=255)

                                                                                                     ccTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                                 <  1:1102115/150‑1 (MQ=21)

                                                                                                       tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAaatttaattt                                               >  1:8372982/1‑150 (MQ=255)

                                                                                                           gTTACTTCCGCTACCTCGTCGATACCTTCAGTGCGGAAGATTTCGGAGTTTGTGGTGCATCCGGGAGGATTCGCA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                           <  1:3084383/150‑1 (MQ=17)

                                                                                                            ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                          >  1:4176469/1‑150 (MQ=255)

                                                                                                             tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATGTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgga                                         <  2:4056669/150‑3 (MQ=255)

                                                                                                                 tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAA‑CCTCAGACCGCACGGATCGTAGCCGAGTACTCTATCCAGCTGAGCTACCGATGCATCGGGAAATTTAGTTTACTGCTGata                                     >  1:2671327/1‑150 (MQ=14)

                                                                                                                   cgcgACCACCTCGAGACCTTCATGGCAGCCGTTTTCGGAGAATATCGTGCATCCGGGAGGATTCGAC‑CCTCCGCCAGCCCGTTCCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                   <  2:861753/150‑1 (MQ=14)

                                                                                                                   cGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                   <  1:2598835/150‑1 (MQ=255)

                                                                                                                   cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                   <  1:1421466/150‑1 (MQ=9)

                                                                                                                    gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGCAATTTATTTTACTGCTGATATTg                                  >  1:6511108/1‑150 (MQ=255)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                 <  2:6611225/150‑1 (MQ=255)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                 <  1:7450106/150‑1 (MQ=255)

                                                                                                                       atctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATa                               <  1:179004/148‑1 (MQ=12)

                                                                                                                        ccTCTTAGATTTCCTCATTGCTGATGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                              <  1:6712928/150‑1 (MQ=14)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                            >  2:4525123/1‑150 (MQ=18)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑TCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                            >  1:986364/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                            >  2:5668018/1‑150 (MQ=255)

                                                                                                                          tctTCGATAACTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                  <  2:485058/96‑1 (MQ=37)

                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATATATTTGACTGCTGATAATGATAcccca                          >  2:2333340/1‑147 (MQ=11)

                                                                                                                             tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                         <  1:4054542/150‑1 (MQ=255)

                                                                                                                             tCGATACCTTCATTGCTGAAGACTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                         <  2:1233050/150‑1 (MQ=255)

                                                                                                                              cGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                       >  2:7335266/1‑135 (MQ=18)

                                                                                                                                  aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                    <  2:1884444/150‑1 (MQ=255)

                                                                                                                                  aCCTTCAGTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                    <  1:5115026/150‑1 (MQ=255)

                                                                                                                                   ccctCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGGTAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg                   <  2:3229478/147‑1 (MQ=255)

                                                                                                                                    cttctgtGCTGAAGTTTTCGGTGATTCTGGTGCATCGTGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGc                  <  1:6876277/144‑1 (MQ=14)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGc                  >  2:1592853/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                         >  2:3762692/1‑126 (MQ=21)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                 <  2:2394400/150‑1 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                 >  2:2988771/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAAACCGCTACTAAAGcc                 >  1:7709560/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGATATTGATAACGCTACTCAAGcc                 >  2:1369345/1‑150 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGATATTTATAACGCTCATATAGCCata              >  1:8181091/1‑150 (MQ=14)

                                                                                                                                         ttGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                                                                                                <  1:4430373/36‑1 (MQ=255)

                                                                                                                                            cTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                    >  2:7592820/1‑140 (MQ=18)

                                                                                                                                            cTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                    <  1:7592858/140‑1 (MQ=18)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg         >  1:4241551/1‑150 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg         <  2:8219541/150‑1 (MQ=255)

                                                                                                                                                 gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAag     >  1:7540094/1‑150 (MQ=255)

                                                                                                                                                  ctttCGGAGAATATGGTGCATCCGTGAGGATTAGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTATATCCAGCTGAGCTAAGGATGCATCGGGAAATTTATTTTACTGCTTAGATTGATACCGCTACTAAAGCCATATCAAGTAaga    <  1:4525129/149‑1 (MQ=14)

                                                                                                                                                   tttCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAagag   <  1:3885657/150‑1 (MQ=255)

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGA  >  NC_000913/2817900‑2818197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`