Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,062 T→A 13.6% intergenic (‑161/+106) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0620TA13.6% 1251.6 / 41.4 470intergenic (‑161/+106)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (123/250);  new base A (72/3);  total (211/259)
Fisher's exact test for biased strand distribution p-value = 2.39e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

AGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTAC  >  NC_000913/3045913‑3046211
                                                                                                                                                     |                                                                                                                                                     
agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCt                                                                                                                                                       >  2:7681498/1‑150 (MQ=255)
agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCt                                                                                                                                                       >  1:1959331/1‑150 (MQ=255)
 gaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTa                                                                                                                                                      >  2:1930547/1‑150 (MQ=255)
    ttcgactattttctaaaggcgcttcggcgcctttttagtcagatgacaaagtacaaaagtgctcagacagtCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAcc                                                                                                                                                   >  2:7071890‑M1/72‑150 (MQ=255)
        taattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCctct                                                                                                                                               >  2:2824269/1‑150 (MQ=255)
         aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                              <  2:7421212/150‑1 (MQ=255)
           ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                            >  1:6295974/1‑150 (MQ=255)
           ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCAccc                                                                                                                                            >  2:5844793/1‑150 (MQ=255)
              gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTCAATATTCGCGCCAGTGCCGGCCTGTTCCCCACCCCCAAACCCACACCCCaa                                                                                                                                         >  1:12018/1‑150 (MQ=39)
              gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCAAACCCACAccccca                                                                                                                                         >  1:9284/1‑148 (MQ=255)
              gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                                                                                         <  1:3426846/150‑1 (MQ=255)
                  aCGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCGAACCCTCACCCCAAAggg                                                                                                                                     >  2:3919601/1‑150 (MQ=255)
                   cGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                                    <  1:2169049/150‑1 (MQ=255)
                    aggcgcttcggcgcctttttagtcagatgacaaagtacaaaagtgctcagacagtCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                                   >  1:8378189‑M1/56‑150 (MQ=255)
                     tATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCTATACCCAAAGGGGCg                                                                                                                                  >  1:4713002/1‑150 (MQ=255)
                     tATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACTCCCCCAAGGGGCg                                                                                                                                  >  2:8176141/1‑150 (MQ=255)
                      aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                 <  1:3948349/150‑1 (MQ=255)
                       tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                <  2:1123690/150‑1 (MQ=255)
                         tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                              >  1:5645239/1‑150 (MQ=255)
                         tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                              >  2:4137354/1‑150 (MQ=255)
                         tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCGAACCCACACCCCCAAAGGGCGCggg                                                                                                                              >  1:7137250/1‑150 (MQ=255)
                         tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACACCCCAAACGGGCGAggg                                                                                                                              >  1:7554278/1‑150 (MQ=255)
                         tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAAACCACTCCCCCAAGGGGCGAggg                                                                                                                              >  1:5817176/1‑150 (MQ=255)
                           tGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCCTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                            <  1:4503705/150‑1 (MQ=255)
                                 tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGCGGGGACTGTCt                                                                                                                      >  2:2723242/1‑150 (MQ=255)
                                    tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                   >  2:7734897/1‑150 (MQ=255)
                                    tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                   <  1:1103600/150‑1 (MQ=255)
                                      aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                                                                                 <  1:4929936/150‑1 (MQ=255)
                                      aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                                                                                 >  2:1395182/1‑150 (MQ=255)
                                       cacccTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCAc                                                                                                                <  1:3798677/146‑1 (MQ=255)
                                       cAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACACCCCAAAGGGGCGCGGGGACAGTCTGAggcc                                                                                                                >  1:7227892/1‑147 (MQ=255)
                                        aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGAGGGGACAGTCTGAGCACt                                                                                                               >  1:2371494/1‑150 (MQ=255)
                                              gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACACCCCAAACGGGCGAGGGGACTGTATGAGCACATTTGTa                                                                                                         >  2:1756508/1‑150 (MQ=255)
                                              gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATACGCGCCAGTGCCGGCCTGTTCCACTCACCCAAACCCACTCCCCCAAGGGGCGCGGGGACGGTCTGAGCACtgttgtc                                                                                                         >  2:1566519/1‑149 (MQ=255)
                                                  aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACACCCCCAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                     >  1:5428472/1‑150 (MQ=255)
                                                  aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAAttt                                                                                                     >  1:2078009/1‑150 (MQ=37)
                                                  aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                     <  2:4188536/150‑1 (MQ=255)
                                                  aaTCTTCAATGTTGGCACGCGTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                     <  2:7742133/150‑1 (MQ=255)
                                                   aTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCGGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTg                                                                                                    >  2:5241552/1‑150 (MQ=255)
                                                     cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                                  <  2:5000416/150‑1 (MQ=255)
                                                      ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAAACCACTCCCCAAAGGGGGGGGGGGACTGTCTGTGCACGTTTGTACGTTTTCa                                                                                                 >  1:7995785/1‑150 (MQ=255)
                                                      ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCAAAACCACTACCCAAAGGGGCGAGGGGACTGTCGGGGCACTTTTGTACTTTGTCa                                                                                                 >  2:7174787/1‑150 (MQ=255)
                                                        cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCTATCCCCCAACGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTGTCATc                                                                                               >  2:3225922/1‑150 (MQ=255)
                                                           tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCTCACCCAAAACCACACCCCAAACGGGCGGGGGGACAGTCGGAGCACGTTTTTACATTGTAAtaaga                                                                                            >  1:4268269/1‑146 (MQ=255)
                                                           tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                            <  2:4705812/150‑1 (MQ=255)
                                                           tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                            <  2:4425306/150‑1 (MQ=255)
                                                            gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                                           <  2:2954130/150‑1 (MQ=255)
                                                               ggCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaa                                                                                        <  2:4313895/150‑1 (MQ=255)
                                                                gCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAACCCAATCCCCAAAGGGGCGAGGGGACAGTCTGATCACATTTGTACATTTTAATCTGACaaaa                                                                                       >  2:6739486/1‑150 (MQ=255)
                                                                 cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaa                                                                                      >  2:5576627/1‑150 (MQ=255)
                                                                  aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                                     <  2:3482255/150‑1 (MQ=255)
                                                                  aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAATaaaaa                                                                                     <  2:6515391/150‑1 (MQ=255)
                                                                   cGCTTGTCCCCCTCGCCCGTTCAGGGAGAGGGCCGGGGGGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                                    <  2:5176363/150‑1 (MQ=255)
                                                                    gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCAACCACACCCCCACCCCCAAGGGGCGCGGGGGCTGTCTGTGCACGTTTGTTATTTTTCAAAAGAATaaaaatg                                                                                   >  1:1721645/1‑148 (MQ=255)
                                                                    gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGCCTGTCTGAGCACGTTTGTACGTTGTCATCTGAATAAAAAgg                                                                                   >  2:1038236/1‑150 (MQ=25)
                                                                      ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcg                                                                                 <  1:1498074/150‑1 (MQ=255)
                                                                      ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCGAAACCACGCCCCACACGGGCGAGGGGCAGGTCTGAGCCCGTTTGGGATTTGTGATATTACTtaaaatggg                                                                                 >  1:7860465/1‑146 (MQ=255)
                                                                       tttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGTGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc                                                                                <  1:3295792/150‑1 (MQ=255)
                                                                       tttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACGAAAAAGgcgc                                                                                >  2:8390902/1‑150 (MQ=255)
                                                                             CCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc                                                                          >  1:2611055‑M1/1‑150 (MQ=255)
                                                                               TCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAAACCGCACCCCAAAGGGGCGAGGGGGCTGTCTGTGGACTTTTTTACTTTTTGATCTGACTAAAAAGGCGCCGAAgcgc                                                                        <  2:2235903‑M1/150‑1 (MQ=255)
                                                                                 gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGCAACCAAAACCACACCCCAAAAGGGCGAGGGGCCGGTCTGTGCACCTTTTTACGTTGTAAAAAGACAAAAAAGGCGCCCAAGccccg                                                                      >  2:2145786/1‑149 (MQ=255)
                                                                                 GCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCTCTCCCCCAAGGGGCGAGGGGACGGGCTGAGCACGGTTGTACGTTGTCAACTGACTAAAAAGGCGCCGAAGCgccg                                                                      <  2:567878‑M1/150‑2 (MQ=255)
                                                                                  CCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCtt                                                                     >  2:3348311‑M1/1‑150 (MQ=255)
                                                                                    CTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                                   >  2:8316078‑M1/1‑150 (MQ=255)
                                                                                      ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                                 <  1:6735476/150‑1 (MQ=255)
                                                                                      gtCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTACCCCTCTCCCCACAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttttta                                                                 <  2:1470834/149‑4 (MQ=255)
                                                                                         aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                              <  1:8251279/150‑1 (MQ=255)
                                                                                         aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                              <  1:5267294/150‑1 (MQ=255)
                                                                                          gggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGCAACCAAACCCAAAACCCAAATGGGGGAGGGGGCTTTCTGAGCACGTTTGTAAATTGTGATATGACTAAAAAAGCGCCCAAGAGCCTGTAGaaaaa                                                             >  1:6558134/1‑149 (MQ=255)
                                                                                           ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                            <  1:2824269/150‑1 (MQ=255)
                                                                                           ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCAACTGACTAAAAAGGCGCCGAAGCGCCGTTAGAAAATa                                                            >  2:6204094/1‑150 (MQ=255)
                                                                                                  gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                                     <  2:3678185/150‑1 (MQ=255)
                                                                                                  gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCAATCCCCAAAGGGGCGACGGGACTGTCAGAGCACTTTTGTACAATTTCATCTGACTAAAAAGGCGCCCAAGCGCCGTTAGCAAATAGACGaaa                                                     >  2:5138762/1‑149 (MQ=255)
                                                                                                    cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGAGGGGCCTGTCTGAGCACGTTTGTACTTTTTCATCTGACTAAAAAGGCGCCGAAGCGCCGGTAGAAAATATTCGAAACa                                                   >  2:8049480/1‑150 (MQ=37)
                                                                                                        gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGa                                               <  1:5656217/150‑1 (MQ=255)
                                                                                                         tGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGaa                                              <  2:4093362/150‑1 (MQ=255)
                                                                                                           aGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCCTCTGACTAAAAAGGCGCCGAAGCGCCGTTAGAAAATAGTCGAATCAGGGACtt                                            >  2:6940794/1‑150 (MQ=38)
                                                                                                              gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                         <  1:4095579/150‑1 (MQ=255)
                                                                                                              gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                         <  2:8110327/150‑1 (MQ=255)
                                                                                                              gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                         >  2:4791022/1‑150 (MQ=255)
                                                                                                              gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCACACCCCCAAGGGGCGAGGGGACTGTCTGAGCCCTTTTGTACTTTTTCATCTGACAAAAAAGGCGCCGAAGCGCCGTTAGAAAATAGTCGAATCAGTGAAATACt                                         >  2:2645110/1‑150 (MQ=255)
                                                                                                                     ttCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTTGTATTc                                  >  2:6077872/1‑150 (MQ=255)
                                                                                                                     ttCGCGCCAGTGCCGGCCTGTTACCCTCACCCTAAACCTAACCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACAGGTATTc                                  >  2:2607837/1‑150 (MQ=255)
                                                                                                                      tCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCGTTAGAAAAAAGTCGAAACAGTGAATTAATGGTAttag                                 >  2:3289345/1‑148 (MQ=38)
                                                                                                                       cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                                <  1:7393040/150‑1 (MQ=255)
                                                                                                                            cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc                           <  1:4326957/150‑1 (MQ=255)
                                                                                                                                     ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                  <  1:2696964/150‑1 (MQ=255)
                                                                                                                                     ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                  <  1:4137354/150‑1 (MQ=255)
                                                                                                                                     ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                  <  1:6114385/150‑1 (MQ=255)
                                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAg               >  2:7012283/1‑150 (MQ=255)
                                                                                                                                          tCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCa             <  2:6295974/150‑1 (MQ=255)
                                                                                                                                            cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa           >  2:6671701/1‑150 (MQ=255)
                                                                                                                                             ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa          <  2:2068278/150‑1 (MQ=255)
                                                                                                                                              cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAATGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAAc         >  1:5993975/1‑150 (MQ=255)
                                                                                                                                               tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACa        >  2:750069/1‑150 (MQ=255)
                                                                                                                                                cACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAg       <  1:6930623/150‑1 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGtt    >  2:7880396/1‑150 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGtt    >  1:8149242/1‑150 (MQ=255)
                                                                                                                                                    cTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTa   <  2:1278321/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTAC  >  NC_000913/3045913‑3046211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: