Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,087,396 T→G 9.7% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,087,3960TG 9.7% 1169.3 / 19.9 452F231V (TTC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (146/250);  new base G (51/5);  total (197/255)
Fisher's exact test for biased strand distribution p-value = 3.18e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 498 total aligned reads displayed.

CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGT  >  NC_000913/3087248‑3087545
                                                                                                                                                    |                                                                                                                                                     
cGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgttt                                                                                                                                                      <  1:6723094/150‑1 (MQ=255)
 gATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc                                                                                                                                                     <  1:681869/150‑1 (MQ=255)
 gATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc                                                                                                                                                     <  1:5898862/150‑1 (MQ=255)
      tGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                <  1:7049101/150‑1 (MQ=255)
      tGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                <  2:7534923/150‑1 (MQ=255)
      tGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAt                                                                                                                                                >  2:2865395/1‑150 (MQ=255)
       gTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc                                                                                                                                               <  2:369250/150‑1 (MQ=255)
       gTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc                                                                                                                                               <  2:4778224/150‑1 (MQ=255)
        tCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCg                                                                                                                                              <  2:3261421/150‑1 (MQ=255)
        tCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCg                                                                                                                                              <  1:8428111/150‑1 (MQ=255)
          gTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGt                                                                                                                                            >  1:7090914/1‑150 (MQ=255)
          gTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGt                                                                                                                                            >  1:6022614/1‑150 (MQ=255)
           tGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTg                                                                                                                                           >  1:7875031/1‑150 (MQ=255)
           tGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTg                                                                                                                                           <  2:4228930/150‑1 (MQ=255)
             cTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGc                                                                                                                                         >  1:5571573/1‑150 (MQ=255)
             cTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGc                                                                                                                                         >  1:619539/1‑150 (MQ=255)
             cTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGc                                                                                                                                         <  2:448273/150‑1 (MQ=255)
              tttCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGcc                                                                                                                                        >  2:4070311/1‑150 (MQ=255)
                  cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAAt                                                                                                                                    <  1:5654133/150‑1 (MQ=255)
                  cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCAAt                                                                                                                                    >  1:7302387/1‑150 (MQ=255)
                     tCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATggg                                                                                                                                 >  2:4671734/1‑150 (MQ=255)
                       aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTg                                                                                                                               >  2:5995996/1‑150 (MQ=255)
                        gCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGa                                                                                                                              <  2:5134990/150‑1 (MQ=255)
                         cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGAc                                                                                                                             >  2:1549533/1‑150 (MQ=255)
                            tctGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGc                                                                                                                          >  2:6142091/1‑150 (MQ=255)
                            tctGAAGAGATCGACCAGAAATCGCTGAAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTACTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGGTATCGGTGGCCCAATGGGTGACttc                                                                                                                          >  1:4137560/1‑148 (MQ=255)
                             ctGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCg                                                                                                                         <  1:3376959/150‑1 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCgg                                                                                                                        >  2:3936010/1‑150 (MQ=255)
                                  gagaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTg                                                                                                                    >  2:4535950/1‑150 (MQ=255)
                                    gaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGAc                                                                                                                  <  1:3215807/150‑1 (MQ=255)
                                     aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACt                                                                                                                 <  2:3515237/150‑1 (MQ=255)
                                       cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTgg                                                                                                               <  2:1008795/150‑1 (MQ=255)
                                        gACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGt                                                                                                              >  1:6419643/1‑150 (MQ=255)
                                          ccAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCg                                                                                                            >  2:3530180/1‑150 (MQ=255)
                                            aGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                          >  1:1376208/1‑150 (MQ=255)
                                            aGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                          >  1:6986548/1‑150 (MQ=255)
                                             gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTaa                                                                                                         <  2:1289587/150‑1 (MQ=255)
                                              aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTaaa                                                                                                        <  2:7358158/150‑1 (MQ=255)
                                                aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAt                                                                                                      >  2:7874620/1‑150 (MQ=255)
                                                  cGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTa                                                                                                    <  1:3468744/150‑1 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATc                                                                                                  <  1:8257458/150‑1 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATc                                                                                                  <  2:7734026/150‑1 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATc                                                                                                  <  2:5523434/150‑1 (MQ=255)
                                                     tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCg                                                                                                 >  2:8330489/1‑150 (MQ=255)
                                                            agCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAc                                                                                          <  1:8120598/150‑1 (MQ=255)
                                                            agCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAc                                                                                          >  1:7612171/1‑150 (MQ=255)
                                                              cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCt                                                                                        <  1:5335243/150‑1 (MQ=255)
                                                                gTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCCAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCTAc                                                                                      >  1:3913845/1‑150 (MQ=255)
                                                                gTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAATTGGTGACTGCGGTCTGACGGGTCGTTAAATTATCTTTGATACCTAc                                                                                      >  1:3728111/1‑150 (MQ=255)
                                                                      gAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACggcggc                                                                                <  2:2495419/150‑1 (MQ=255)
                                                                      gAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAACTACggcggc                                                                                >  2:6677765/1‑150 (MQ=255)
                                                                             tcatcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGgcgc                                                                         <  1:241034/150‑1 (MQ=255)
                                                                               atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGt                                                                       >  2:1617949/1‑150 (MQ=255)
                                                                                   aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCt                                                                                        >  2:6479132/1‑129 (MQ=255)
                                                                                   aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCAATGGGTGTCGGCGGTCTGTCTGGGCGTAAAATTATAGTTGATAACTACGGCGGCATGGCGCGGCACg                                                                   >  1:2141593/1‑150 (MQ=255)
                                                                                     ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTCTCGGTGGCCCAATGTGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGt                                                                 <  1:668265/150‑1 (MQ=255)
                                                                                     ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGt                                                                 <  2:1415425/150‑1 (MQ=255)
                                                                                       aaTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTgg                                                               <  2:8143073/150‑1 (MQ=255)
                                                                                       aaTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTgg                                                               <  1:1980074/150‑1 (MQ=255)
                                                                                       aaTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTgg                                                               <  1:4792624/150‑1 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGTCTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCg                                                             >  1:5444222/1‑150 (MQ=255)
                                                                                            tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTg                                                          >  2:2717034/1‑150 (MQ=255)
                                                                                             gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGc                                                         <  2:3373766/150‑1 (MQ=255)
                                                                                                    gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTctct                                                  <  1:1831377/150‑1 (MQ=255)
                                                                                                    gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTctct                                                  <  2:631777/150‑1 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTg                                                 <  1:4440667/150‑1 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTg                                                 <  2:4315369/150‑1 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAAAGGGTGACTGCGGTCTGAGGGGTCGGAAAAATATAGTTGTTAACTCCCGCGGCATGGCGCGGCACGGTGGGGGTGCaggcgctg                                                 >  1:7474827/1‑143 (MQ=255)
                                                                                                      aTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTgg                                                <  1:8026047/150‑1 (MQ=255)
                                                                                                         gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCCTTATCGGTGGCCCAAAGGGTGACTTCGGTCTGACAGGTCGTAAAAAAAACGTTTATACCAAACGCGGCAATGCGCGTCACGCTGGCGGTGCAATCACAGGTaa                                             >  2:967527/1‑150 (MQ=255)
                                                                                                          cTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTTTAGGTGGCCCAATGGGTGTCTGCGGTCTGCCTGGTCGTAAAAATATAGTTTATAACAAACGCCGCAAGGCGCGTCACCGTGGCGGTGCATTCACTGGTaaa                                            >  1:2764026/1‑150 (MQ=255)
                                                                                                          cTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaa                                            <  1:2657264/150‑1 (MQ=255)
                                                                                                           tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaat                                           >  2:1937755/1‑149 (MQ=255)
                                                                                                           tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAAAGGGTGACTGCGGTCTGTCAGGTGGTAAAAATATAGGTTTTAACAACCGCGGCAAGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAg                                           >  1:1524208/1‑150 (MQ=255)
                                                                                                             aCTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGAt                                         <  1:1103434/150‑1 (MQ=255)
                                                                                                              cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATc                                        <  2:7944842/150‑1 (MQ=255)
                                                                                                              cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATc                                        >  2:6910200/1‑150 (MQ=255)
                                                                                                               ttCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATcc                                       <  2:7184277/150‑1 (MQ=255)
                                                                                                               ttCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTAGTTATGGGTGGCCCAATGGGTGACAGCGGTCTGTCTGGTGGTAAAAATATAGTTTTTAAATAAGGCGGCAATGCGCGTCACGGTGGCGGTGCATTCTCAGGTAAatatac                                       >  1:3082841/1‑148 (MQ=255)
                                                                                                                 cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGTGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGTCTGTGCATTCTCTGGTAAAGATCCAt                                     <  1:5075992/150‑1 (MQ=255)
                                                                                                                 cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCAt                                     <  1:7657099/150‑1 (MQ=255)
                                                                                                                    ccaccaAATTCTTCATCAACCCGACCGGTCGTGGGGTTATAGGGGGCCCAAAGGGTGTCAGCGGGCTGACAGGGCGTAAAAATATATTTGATAAAAAAGGCGGCAATGCGCGTCACGGGGGCGGGGCATTCTCTGGTGAAAAGCcaacaa                                  >  1:4306985/1‑150 (MQ=255)
                                                                                                                        caAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGt                              <  2:5840317/150‑1 (MQ=255)
                                                                                                                             tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAcc                         <  2:2048899/150‑1 (MQ=255)
                                                                                                                             tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGGGCATTCTCTGGTAAAGATCCATCAAAAGTGGAcc                         >  2:2356609/1‑150 (MQ=255)
                                                                                                                              cttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCg                        <  1:4653612/150‑1 (MQ=255)
                                                                                                                              ctacatcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCg                        <  1:2477357/147‑1 (MQ=255)
                                                                                                                               ttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGt                       >  2:3044643/1‑150 (MQ=255)
                                                                                                                               ttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACGGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGt                       >  2:5219572/1‑150 (MQ=255)
                                                                                                                                  atcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTcc                    >  2:7648168/1‑150 (MQ=255)
                                                                                                                                   tcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCg                   <  2:8402056/150‑1 (MQ=255)
                                                                                                                                   tcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCg                   >  2:5931343/1‑150 (MQ=255)
                                                                                                                                    caACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGc                  <  2:5335630/150‑1 (MQ=255)
                                                                                                                                      aCCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGGTGATAACTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAACGTTCCGCAg                >  2:5743249/1‑150 (MQ=255)
                                                                                                                                              ggtcgggtcgTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgca        <  2:6986548/150‑1 (MQ=255)
                                                                                                                                              ggTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgca        <  2:1104712/150‑1 (MQ=255)
                                                                                                                                                   tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACg   >  1:2353062/1‑150 (MQ=255)
                                                                                                                                                   tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACg   >  1:649992/1‑150 (MQ=255)
                                                                                                                                                    ttcgttATCGGTGGCCCACTGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGt  <  2:4933783/150‑1 (MQ=255)
                                                                                                                                                    ttcggtATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGt  <  2:2993434/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGT  >  NC_000913/3087248‑3087545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: