Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,709 A→C 5.5% intergenic (+26/+19) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,7090AC 5.5% 1141.8 / 2.5 400intergenic (+26/+19)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (209/147);  new base C (2/32);  total (219/181)
Fisher's exact test for biased strand distribution p-value = 8.16e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

GCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233560‑3233858
                                                                                                                                                       |                                                                                                                                                      
gcgggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                                                                        <  2:5787546/147‑1 (MQ=255)
gcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                                                                        <  2:4333543/150‑1 (MQ=255)
 cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  2:3341305/150‑1 (MQ=255)
  tGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACg                                                                                                                                                     >  1:4599846/1‑150 (MQ=255)
  tGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACg                                                                                                                                                     <  2:6793090/150‑1 (MQ=255)
      gcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATc                                                                                                                                                 >  1:1418598/1‑150 (MQ=255)
         aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCgg                                                                                                                                              >  1:4218512/1‑150 (MQ=255)
            gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCAc                                                                                                                                           >  1:5751348/1‑150 (MQ=255)
            gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCAc                                                                                                                                           >  1:8411072/1‑150 (MQ=255)
            gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCAc                                                                                                                                           >  2:4972385/1‑150 (MQ=255)
                attgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGTAACGCATCCGGCACGTcg                                                                                                                                       >  2:1833726/1‑150 (MQ=255)
                   gatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGt                                                                                                                                    >  2:875852/1‑150 (MQ=255)
                       gCGGTAAAACTGTGCATTTATTTGGGGGCTGCTTTGTGGCTTTGGAGCTGGACGCACGACGGGCATTTGCCCTGGGAACACGGCGGGCGCTGTTTATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAAt                                                                                                                                <  2:4232954/150‑1 (MQ=255)
                       gCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCCATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTGATTTGAAGCGCCGGATGCACCGCATCCGGCACGTCGCGTTacg                                                                                                                                >  1:6910473/1‑148 (MQ=255)
                       gCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAAt                                                                                                                                >  2:4088281/1‑150 (MQ=255)
                        cGGTAAAAGGGTGCTTTTATTTGGGGGCTGCGTTGTGGCTAGGGAGCTGGACGCACGAGGGGCATTGGCCCCGGGAACCCGGGTGGCGGTGGAGTTTTAAATCGTTATTTGAAGCGCCGTATGCAACGAATCCGGCACGTCGCGTTAATc                                                                                                                               <  2:398088/150‑1 (MQ=255)
                        cGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATc                                                                                                                               >  1:4415889/1‑150 (MQ=255)
                                  gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGACCACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                     >  1:5626433/1‑150 (MQ=255)
                                  gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCGGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTCAATCAATAAACAAg                                                                                                                     <  2:7027434/150‑1 (MQ=255)
                                   tGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAgg                                                                                                                    >  1:1999959/1‑150 (MQ=255)
                                   ggcttttATTGGGGGGCTGGGATGTGGCTTGGGTGGGGGACGCCGGCCGGGAATTTGCCCAGGGAACTCGGCGGGGGCTGTAGTTTTTATTGGTTATTTGACGCGCGGTTTGCAACGCATCGGGCACGTCGCGTTAATCAATTAACAAgg                                                                                                                    <  2:5872686/146‑1 (MQ=255)
                                       tttAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCggg                                                                                                                >  1:993867/1‑150 (MQ=255)
                                             tGGCGGCTGCGTTGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGTTTTAAATCGTTATTTGACGCGCCGGATGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAt                                                                                                          <  2:8352797/150‑1 (MQ=255)
                                                  gcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGa                                                                                                     >  1:688806/1‑150 (MQ=255)
                                                       gttggggCTATGGAGCTGGCCGCACGCCGGGATATTGCCCAGGCAACCCGGCTGGCGCTGTATTTTTAAATCTTTATTTTAAGCGCCGGAGGCAACGAATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATcgc                                                                                                <  2:7953206/145‑1 (MQ=255)
                                                       gATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATcgc                                                                                                >  1:8033564/1‑150 (MQ=255)
                                                            ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                           >  1:6363437/1‑150 (MQ=255)
                                                               tctGGTGCTGGACGCACGCCGGGAATTTGCCCTGGTAACCCGGCTGGGGCTGTATATTTAAATGGTTTTTTGACGCGCCGTTTGAAAGGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCaa                                                                                        <  2:2357771/148‑1 (MQ=255)
                                                               tATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCaa                                                                                        >  1:122614/1‑150 (MQ=255)
                                                               tATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCaa                                                                                        >  2:8231716/1‑150 (MQ=255)
                                                                ctGGCGCTGGACGCCCGCCGGGCAATTGCCCTGGGACCACGGCTGGCGGGTTAGATTTAAATCGTTATTTCAAGCGCCGCAGGCCACGCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       <  2:1227786/149‑1 (MQ=255)
                                                                 tggcgggggACGCACGACGGGCAATGGCCCAGGTACCCCGGCGGGCGCTGGAGATTTAAATCGTTCTTTGACGCGCCGGAGGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtt                                                                                      <  2:5526853/143‑1 (MQ=255)
                                                                 tGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtt                                                                                      >  2:5141563/1‑150 (MQ=255)
                                                                 tGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAAAAACAAGGCGGGAGAAAAGCCGAATCGCGTTGCCAAtt                                                                                      >  2:4549705/1‑150 (MQ=255)
                                                                 tGGAGCAGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAGGCCGAATCGCGTTGCCAAtt                                                                                      >  2:8164965/1‑150 (MQ=255)
                                                                   gAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt                                                                                    >  2:3135983/1‑150 (MQ=255)
                                                                     gCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttttt                                                                                  >  1:264258/1‑150 (MQ=255)
                                                                      cTGGCCGCACGCCGGCAATTGCCCCTGGTAACACGGCTGGCGGTGGATATTAAAATGTTTTTTGGACGCGCCGGATGAACCCCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 <  1:2744727/150‑1 (MQ=255)
                                                                        ggACGACCGACGGGCATTGGCCCTGGTCACCCGGCGGGCGGGGGTGATTTAAATCGGTTTTTGACGCGCGGGTTGCAACGCTTCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                                                                               <  2:6757118/150‑1 (MQ=255)
                                                                         gACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCCATTTTTTAGCg                                                                              >  2:5365016/1‑150 (MQ=255)
                                                                         gACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCg                                                                              >  1:782342/1‑150 (MQ=255)
                                                                         gACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCg                                                                              >  2:6908699/1‑150 (MQ=255)
                                                                          ccGCACGCCGGGATCTGGCCCAGGGACCACGGGCGGCGGGGGAGATTAAAAGCGTTTTTTCCCGCGCGGGATCCAACCCAGCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgt                                                                             <  2:3028929/149‑1 (MQ=255)
                                                                          ccGCACGACGGGCATTTGCAAAGGGAACACGGGGGGCGCTGTAGATTTAAATGGTTATTTGAAGCGCCGGAGGCACCGCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgt                                                                             <  2:1989988/149‑1 (MQ=255)
                                                                            gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  2:6364896/1‑150 (MQ=255)
                                                                            gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  1:2220028/1‑150 (MQ=255)
                                                                                 ccgGGCATTTGCCCAGGGAACACGGCTGGCGCTTGTCTTTTAAATCGTTTTTTGAGGCGCCGGTTGCAAGGAATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                      <  2:7591270/149‑1 (MQ=255)
                                                                                  cgGGCCTTTGCCCTGGGAACCCGGCGGGCGCTGTAGTTTTAAATCGTTATTGGCCGCGCGGGATGCACCGCAGCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGc                                                                     <  2:8071459/150‑1 (MQ=255)
                                                                                  cgGGCAAGTGCCCCGGCACCCCGGCTGGCGGGTTTTATTTAAAGCGTTATTGCACGCGCGGGAGCAACCCCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGc                                                                     <  1:3083722/150‑1 (MQ=255)
                                                                                   gggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCg                                                                    <  2:5579070/150‑1 (MQ=255)
                                                                                    gtaattggCCCTGGGACCCCGGCGGGCGCTGGATATTTAAATCTTTATTGGCCGCGCCGGCTGCACCGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGt                                                                   <  2:7112301/143‑1 (MQ=255)
                                                                                    ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGt                                                                   >  1:831968/1‑150 (MQ=255)
                                                                                     gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:2539333/1‑150 (MQ=255)
                                                                                      cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTa                                                                 >  2:1511492/1‑150 (MQ=255)
                                                                                      cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTa                                                                 >  2:2080361/1‑150 (MQ=255)
                                                                                      cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTa                                                                 >  2:7470912/1‑150 (MQ=255)
                                                                                       aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTaa                                                                >  2:4012844/1‑150 (MQ=255)
                                                                                           gCCCTGGGAACCGGGGTGGCGGTGGTTATTTAACTCTTTATTTGACGCGCGGGTTGCACCGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc                                                            <  1:1054545/150‑1 (MQ=255)
                                                                                           gCCCAGGCAACCGGGCGGGCGCTGGAGATTAAACTCGTTTTTTGCCGCGCCGGATGCAACGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc                                                            <  1:2437413/150‑1 (MQ=255)
                                                                                               ctgggAACCCGGCTGGCGCTGGATTTTTAAATCGTTATTTGAAGCGCCGGATGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctc                                                         <  1:6275270/148‑1 (MQ=255)
                                                                                               cAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctc                                                         >  1:6646746/1‑150 (MQ=255)
                                                                                                aGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctct                                                        >  2:7403858/1‑150 (MQ=255)
                                                                                                aGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctct                                                        >  1:7442157/1‑150 (MQ=255)
                                                                                                  ggAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTc                                                      >  1:717665/1‑150 (MQ=255)
                                                                                                   taaCAGGGCTGGCGCTGTTTTTTAAAATCTTTATTTGACGCGCCGGAGGCAACGCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTcc                                                     <  1:1839338/149‑1 (MQ=255)
                                                                                                   gAACACGGCTGGCGCTGGATATTTAAATCGTCATTGGACGCGCCGGATGCCACGCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTcc                                                     <  2:475048/150‑1 (MQ=255)
                                                                                                     acacGGCTGGCGCTGGAGATTTAAATCGTTATTTGACGCGCCGGATGAACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCg                                                   <  2:7682217/150‑1 (MQ=255)
                                                                                                               cgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc                                         >  1:4269149/1‑150 (MQ=255)
                                                                                                                gcTGGATTTTTAAATCGTTATTTGAAGCGCCGTATGAAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgcg                                        <  2:6162925/150‑1 (MQ=255)
                                                                                                                  ggtttttttAAATTGTTTTTTTCACGCGCGGGATGCAACGCCCCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt                                       <  2:2856149/144‑1 (MQ=255)
                                                                                                                    gagaTTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGac                                    >  2:2311153/1‑150 (MQ=255)
                                                                                                                     agaATTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGaca                                   >  2:5092723/1‑150 (MQ=255)
                                                                                                                          tAAATCGTTTTTTGACGCGCCGTATGAAACGCAACCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                              <  1:5960094/150‑1 (MQ=255)
                                                                                                                             aTCGTTATTTGAAGCGCCGGATTCAACGCAAACCGCACGGCGCGTTAATAAAAAAAAAAGGCGGGAGAAATTCCCAAAAGCGTTGCCAATTTTTTAGCGTGTTGCAGCGTTAAATTACTCTTACCCCACAAAAACCCTGACACCAAAGAt                           >  1:3061223/1‑150 (MQ=255)
                                                                                                                                gTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTGCTCTTCCCGCTCAAAACGCGTGTCACCATAGATTTa                        >  2:2955707/1‑150 (MQ=255)
                                                                                                                                  ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       >  2:7280824/1‑150 (MQ=255)
                                                                                                                                  ttATTTGAAGCGCCAGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       >  2:1742147/1‑150 (MQ=255)
                                                                                                                                    aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                     <  1:2834085/150‑1 (MQ=255)
                                                                                                                                      ttGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGcc                   >  2:1346960/1‑150 (MQ=255)
                                                                                                                                        gacgcgcCGTATGCAACGCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:3100549/147‑1 (MQ=255)
                                                                                                                                        gacgcgcCGGATGCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:3504010/147‑1 (MQ=255)
                                                                                                                                         acgtgcCGGATGCGTTGCATCCGGCGCGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTACCGCGCAAAACGCGTGACACCATATATTTACAGCCAAt                >  2:2129780/5‑150 (MQ=255)
                                                                                                                                           gcgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt              >  1:331896/1‑150 (MQ=255)
                                                                                                                                             gcCGGATGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc            <  1:4355635/150‑1 (MQ=255)
                                                                                                                                              ccGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCg           >  2:4840919/1‑150 (MQ=255)
                                                                                                                                              ccGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCg           >  2:753928/1‑150 (MQ=255)
                                                                                                                                               cGGATGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          <  1:6498371/150‑1 (MQ=255)
                                                                                                                                               cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          <  2:3436333/150‑1 (MQ=255)
                                                                                                                                               cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          >  1:481568/1‑150 (MQ=255)
                                                                                                                                                 gATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                                                                               <  2:5268931/79‑1 (MQ=255)
                                                                                                                                                 gATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                                                                               >  1:5268931/1‑79 (MQ=255)
                                                                                                                                                  aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg       >  1:1652305/1‑150 (MQ=255)
                                                                                                                                                    gCACCGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGAt     <  2:3588735/150‑1 (MQ=255)
                                                                                                                                                    gCAACGCATCCGGCACGTCGCGTTAATCAAAAAACAAGGCGGGAGAAAAGCCGAATCGCGTAGCCAATTTTATAGCGAGTTCCAGCGTAAATTTCCTCTTAACGCTCAAAACTCGTGACACCATATATATACTGCCAATATCAGGCAGaa     >  1:486478/1‑149 (MQ=255)
                                                                                                                                                    gCAACCCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGAt     <  1:2311566/150‑1 (MQ=255)
                                                                                                                                                     cAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATc    >  1:5352565/1‑150 (MQ=255)
                                                                                                                                                     cAACGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATc    <  2:5985438/150‑1 (MQ=255)
                                                                                                                                                      aaCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg   >  1:5108487/1‑150 (MQ=255)
                                                                                                                                                      aaCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg   >  1:392920/1‑150 (MQ=255)
                                                                                                                                                       aCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCgg  >  1:3770229/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233560‑3233858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: