Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,391,407 A→C 15.3% F208V (TTT→GTT)  tldD ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,391,4070AC15.3% 1689.4 / 108.1 657F208V (TTT→GTT) tldDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (381/161);  new base C (4/109);  total (386/271)
Fisher's exact test for biased strand distribution p-value = 2.07e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 686 total aligned reads displayed.

CGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGC  >  NC_000913/3391258‑3391556
                                                                                                                                                     |                                                                                                                                                     
cGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCaaa                                                                                                                                                       <  2:7961679/150‑1 (MQ=255)
 ggCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCaaaa                                                                                                                                                      <  1:5301481/150‑1 (MQ=255)
    cAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAGACGa                                                                                                                                                   <  2:1415553/150‑1 (MQ=255)
    cAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGa                                                                                                                                                   <  2:3492971/150‑1 (MQ=255)
      aCCTGCGCCATGTAATACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAATTATTGACCAGCGCAATACGAACTGCTTCTTTTGCCAATGCATCGGAACGGACTTCGCCGTCTAGATCGGAATGTAATAATTAATAACCAAAACGAcc                                                                                                                                                 <  2:7829143/150‑1 (MQ=255)
        cTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgc                                                                                                                                               >  1:7675456/1‑150 (MQ=255)
        cTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgc                                                                                                                                               >  1:4157057/1‑150 (MQ=255)
               aaGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGACGAATTCATAACCAAAACGACCgccgccgcc                                                                                                                                        >  2:3273028/1‑150 (MQ=255)
                aGTACTCCGGGCTTGGGGCCCGCTGGTGCAGCACCGGCTGAAAGTTTGACCCGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGATTTCATAACCAAAACGACCGCCGCCGCCa                                                                                                                                       <  2:215573/150‑1 (MQ=255)
                    ctacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCAGAACCAAAACGACCGCCGCCGCCACTgg                                                                                                                                   >  2:3629517/1‑150 (MQ=255)
                     tacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGc                                                                                                                                  >  1:1701873/1‑150 (MQ=255)
                       ccGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTGGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGTAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCAc                                                                                                                                <  2:2995891/150‑1 (MQ=255)
                          gCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgc                                                                                                                             >  2:5570580/1‑150 (MQ=255)
                           cATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcg                                                                                                                            >  1:3617656/1‑150 (MQ=255)
                             tGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgtt                                                                                                                          >  1:1879068/1‑150 (MQ=255)
                               gTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcg                                                                                                                        >  1:6044773/1‑150 (MQ=255)
                                   ccGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgcgtt                                                                                                                    >  2:2237612/1‑150 (MQ=255)
                                   ccGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgcgtt                                                                                                                    >  1:5179560/1‑150 (MQ=255)
                                   ccGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgcgtt                                                                                                                    >  2:103684/1‑150 (MQ=255)
                                    cGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGttt                                                                                                                   <  2:1483130/150‑1 (MQ=255)
                                    cGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCATTCGCGttt                                                                                                                   >  1:6859530/1‑150 (MQ=255)
                                     gCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTg                                                                                                                  >  2:8353882/1‑150 (MQ=255)
                                      cTGGTGCAGCAACGGCAGAAAGATTGACCCGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGc                                                                                                                 <  2:5966619/150‑1 (MQ=255)
                                          tGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtc                                                                                                             <  2:6907958/150‑1 (MQ=255)
                                          tGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtc                                                                                                             >  2:4631725/1‑150 (MQ=255)
                                           gcagcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtct                                                                                                            <  2:4929355/150‑1 (MQ=255)
                                                    gCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                                   >  2:4293994/1‑150 (MQ=255)
                                                    gCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                                   >  2:18752/1‑150 (MQ=255)
                                                     cataaaTATTGACCAGCGCAATACGCACTGTTTCTTTTGCCCAGGCATCCGCACGGACTTCGCCGTCGAGTTCGGATCGTAATAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacga                                                                                                  <  1:882439/147‑1 (MQ=255)
                                                      aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                 >  1:3250411/1‑150 (MQ=255)
                                                       gAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCAACCGCACGTACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgaga                                                                                                <  2:2953278/150‑1 (MQ=255)
                                                        aaaGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGaa                                                                                               >  1:4299294/1‑150 (MQ=255)
                                                         aaGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTGCGCCGGCGAGAGCGGCACGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                              <  2:729423/150‑1 (MQ=255)
                                                            aTTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCt                                                                                           >  1:1134170/1‑150 (MQ=255)
                                                             ttGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTc                                                                                          >  2:6191699/1‑150 (MQ=255)
                                                             ttGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTc                                                                                          >  2:6650846/1‑150 (MQ=255)
                                                             ttGACCAGCGCCATACGCACTGATTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCTAGATCGCAAAGTAAGAATTCATAACCACAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTc                                                                                          <  2:6434270/150‑1 (MQ=255)
                                                              tGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCa                                                                                         >  2:2655373/1‑150 (MQ=255)
                                                              tGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCa                                                                                         >  2:2095601/1‑150 (MQ=255)
                                                               gACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCAc                                                                                        >  2:6601105/1‑150 (MQ=255)
                                                                aCCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                       >  1:7426513/1‑150 (MQ=255)
                                                                aCCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                       >  1:2295052/1‑150 (MQ=255)
                                                                     cgcCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAg                                                                                  >  1:4665320/1‑150 (MQ=255)
                                                                      cccacccGCATTTCTTCTTTTGCCCCCCCACCCGCACGGACTGCGCCGTCGAGATCGGCATGTAAGAATGCATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGa                                                                                 <  1:2196101/144‑1 (MQ=255)
                                                                           actcactGCTTCTTTGGCCCTTGCATCCGCACGGACTTCGCCGTCGAGATGGGAATGTAAGATTTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCAC                                                                            <  1:1245481‑M1/147‑1 (MQ=255)
                                                                           aCGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCAC                                                                            <  2:4239657‑M1/150‑1 (MQ=255)
                                                                              cACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAg                                                                         >  1:7196826/1‑150 (MQ=255)
                                                                                cTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcg                                                                       >  2:7607805/1‑150 (MQ=255)
                                                                                   cttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggac                                                                    >  1:5971884/1‑150 (MQ=255)
                                                                                   cttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggac                                                                    >  1:4848348/1‑150 (MQ=255)
                                                                                    ttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacg                                                                   <  2:7354147/150‑1 (MQ=255)
                                                                                    ttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacg                                                                   >  2:5144541/1‑150 (MQ=255)
                                                                                     tcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacgg                                                                  >  2:3300315/1‑150 (MQ=255)
                                                                                      cttTTGCCCTTGCATCCGCAGGGACTTCGCCGTCGTGTTCGGCATGTAATAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacgga                                                                 <  1:5868742/150‑1 (MQ=255)
                                                                                       ttttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCggacggac                                                                >  2:5728148/1‑150 (MQ=255)
                                                                                          tGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATc                                                             <  1:5512797/150‑1 (MQ=255)
                                                                                          tGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATc                                                             >  1:3951634/1‑150 (MQ=255)
                                                                                                tGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCcgccgc                                                       <  1:6342470/150‑1 (MQ=255)
                                                                                                 gCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCt                                                      >  2:8440505/1‑150 (MQ=255)
                                                                                                    tCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                                   >  1:918132/1‑150 (MQ=255)
                                                                                                        cACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCgtgc                                               >  2:5067072‑M2/1‑146 (MQ=255)
                                                                                                         aCGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGcc                                              <  2:4741294/150‑1 (MQ=255)
                                                                                                             aCTTCGCGGTCGAGATGGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                          <  2:168545/150‑1 (MQ=255)
                                                                                                              cTTCGCCGTCGAGATCGGCAAGTAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCgg                                         <  1:8141098/150‑1 (MQ=255)
                                                                                                               ttCGCCGTCTAGATCGGCATGGAATAATTAATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        <  1:8281202/150‑1 (MQ=255)
                                                                                                               ttCGCCGTCGAGTTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGATGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        <  2:116647/150‑1 (MQ=255)
                                                                                                               ttCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        >  1:3371965/1‑150 (MQ=255)
                                                                                                               ttCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        >  1:7151714/1‑150 (MQ=255)
                                                                                                               ttCGCCGTCGAGAGCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        <  2:2555005/150‑1 (MQ=255)
                                                                                                                tCGCCGTCGAGATCGGCAAGGAAGATTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTg                                       <  1:2052838/150‑1 (MQ=255)
                                                                                                                   ccGTCGAGATCGGCAAGTAAGAATTCAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGcc                                    <  1:1604046/150‑1 (MQ=255)
                                                                                                                     gTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTCGCGTGCCGTCGGTGGCCGc                                  >  2:7164456/1‑150 (MQ=255)
                                                                                                                       cGAGATCGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCaa                                <  1:2770878/150‑1 (MQ=255)
                                                                                                                       cGAGATCGGAAAGGAAGAATTAATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCaa                                <  1:4991519/150‑1 (MQ=255)
                                                                                                                          gaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCa                             >  2:3798631/1‑150 (MQ=255)
                                                                                                                            tCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGACCGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCACCCaaa                           >  2:3114318/1‑150 (MQ=255)
                                                                                                                            tCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCACCCaaa                           >  1:6463300/1‑150 (MQ=255)
                                                                                                                            tCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaa                           >  2:1936183/1‑150 (MQ=255)
                                                                                                                               gCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatt                        >  1:8433753/1‑150 (MQ=255)
                                                                                                                                cAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatta                       >  2:4520333/1‑150 (MQ=255)
                                                                                                                                 aaGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                      <  2:5220674/150‑1 (MQ=255)
                                                                                                                                 aaGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCACCCAAaattaa                      >  2:81693/1‑150 (MQ=255)
                                                                                                                                  aGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaat                     <  2:6818158/150‑1 (MQ=255)
                                                                                                                                  aGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaat                     <  1:2582050/150‑1 (MQ=255)
                                                                                                                                  aGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCACAACCAAaattaat                     <  2:2779700/150‑1 (MQ=255)
                                                                                                                                   gtaagaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaatt                    <  1:3164373/148‑1 (MQ=255)
                                                                                                                                   ggAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaatt                    >  1:672534/1‑150 (MQ=255)
                                                                                                                                      agaaTTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGGCGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCAt                 <  1:3516109/150‑1 (MQ=255)
                                                                                                                                       taaTACATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATa                <  1:7937440/149‑1 (MQ=255)
                                                                                                                                       gaaTTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATa                <  2:5459660/150‑1 (MQ=255)
                                                                                                                                         aTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGa              >  1:1038257/1‑150 (MQ=255)
                                                                                                                                          ttCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac             >  2:3869053/1‑150 (MQ=255)
                                                                                                                                            cATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGacac           <  2:3718062/150‑1 (MQ=255)
                                                                                                                                             aTAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcacc          >  2:1637481/1‑150 (MQ=255)
                                                                                                                                              tAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcacca         <  2:7035929/150‑1 (MQ=255)
                                                                                                                                               aaCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCACCCAAAATTAATTCATAGAcaccac        >  1:37960/1‑150 (MQ=255)
                                                                                                                                                 ccAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTg      >  1:248/1‑150 (MQ=255)
                                                                                                                                                  cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCCACCAAAATTAATTCATCGACACCACTGa     >  2:2426652/1‑150 (MQ=255)
                                                                                                                                                  cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa     >  1:3006242/1‑150 (MQ=255)
                                                                                                                                                  cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa     >  2:4001360/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGC  >  NC_000913/3391258‑3391556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑