Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,067 T→G 11.2% intergenic (+50/‑148) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0670TG11.2% 1031.2 / 24.9 363intergenic (+50/‑148)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (131/178);  new base G (49/2);  total (183/180)
Fisher's exact test for biased strand distribution p-value = 3.52e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

AAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAT  >  NC_000913/3780919‑3781216
                                                                                                                                                     |                                                                                                                                                     
aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                                      <  1:3500860/150‑1 (MQ=255)
aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGGAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                                      <  2:5625945/150‑1 (MQ=255)
  aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCCCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                                    >  1:5487961/1‑150 (MQ=255)
   tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                                                   <  2:3928897/150‑1 (MQ=255)
    cGATCAGCGAAAGGACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                                                  <  2:5838577/150‑1 (MQ=255)
     gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAACCCACACCCACAGGGAGAGGGGACCGTTCGgcg                                                                                                                                                 >  1:4882806/1‑150 (MQ=255)
      aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTGGCCTGCGGCACTGGCTCCCATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                                                <  1:6409624/150‑1 (MQ=255)
       tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                               <  1:5764267/150‑1 (MQ=255)
       tCAGCGAAAGTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGACAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                               <  2:4808929/150‑1 (MQ=255)
          gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCCAAACCACAACCACCCGGGGCGGGGACCCTTCGgcgcggga                                                                                                                                            >  1:4982323/1‑146 (MQ=255)
          gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACACCCACACGGAGAGGGGACCCTTAGGCGCtgta                                                                                                                                            >  2:503492/1‑150 (MQ=255)
          gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACAACCACAGGGGGAGGGGACCCGTCGGCGCggta                                                                                                                                            >  1:377705/1‑150 (MQ=255)
           cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                                           <  1:5009979/150‑1 (MQ=255)
            gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                                                                          <  2:4705286/150‑1 (MQ=255)
              aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                                                                        <  1:8232218/150‑1 (MQ=255)
                ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACt                                                                                                                                      <  2:4369157/150‑1 (MQ=255)
                    gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                                                  <  2:448434/150‑1 (MQ=255)
                    gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTCCCACCGGGGGAGGGGACCGTTCGGCGCTGTATGTACTacca                                                                                                                                  >  1:8351162/1‑149 (MQ=255)
                    gCAAGATTCGCTGGTGAAGGGGCTGGTTAAAGAGTTGCCTGAGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTGACCCGCTCCCTCCGGGGGGGGGGGCCGGGCGGGGCTGGATGGGCTCCCt                                                                                                                                  >  2:553686/1‑150 (MQ=255)
                     cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACAACCAAACCCAAAACCACAGGGAGGGGGGACCCTTCGGCGCGGGTTGTAcaaccgc                                                                                                                                 >  2:574949/1‑144 (MQ=255)
                      aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCGAAACCACACCCTCCCGGGGTGGGGACCCTTCGGCGCGGGATGTACTCCcaca                                                                                                                                >  2:524379/1‑150 (MQ=255)
                       aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAc                                                                                                                               <  1:2632909/150‑1 (MQ=255)
                        gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                                                                              <  2:6981717/150‑1 (MQ=255)
                         aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                                                                             <  2:2842981/150‑1 (MQ=255)
                          ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACAGGGGGAGGGGGCCCGGCGGCGCGGGAGGTACACCCacacaca                                                                                                                            >  1:150935/1‑147 (MQ=255)
                             gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                                                                         <  2:165306/150‑1 (MQ=255)
                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                        <  1:2346293/150‑1 (MQ=255)
                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                        <  2:6294815/150‑1 (MQ=255)
                               tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                                       <  1:5823291/150‑1 (MQ=255)
                                 gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                                                     <  1:5325376/150‑1 (MQ=255)
                                 gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTCCCACAGGGAGAGGGGCCCGGTCGGCGCTGTATGTACACCCGCACACTGAAACGa                                                                                                                     >  1:5141586/1‑150 (MQ=255)
                                   gCAGGGGCTGGTTAAAGTGTTGCCTGCGGCACTGGCTCCCATGGCTAATGGGATTGCGGCTTTGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGaca                                                                                                                   <  1:560516/150‑1 (MQ=255)
                                        ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                              <  2:7913608/150‑1 (MQ=255)
                                             ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtt                                                                                                         <  2:1532510/150‑1 (MQ=255)
                                             ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAAACCACTCCCTCAGGGTGTGGGGACCGGTAGGGGGGGTTTGTACTGCCTCACTCTGAAACCACACCGcccaatt                                                                                                         >  2:5087825/1‑144 (MQ=255)
                                               tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTGGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                       <  1:1023524/150‑1 (MQ=255)
                                               tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                       <  1:5410831/150‑1 (MQ=255)
                                               tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCAAACCCCCACCCTCAGGGGGTGGGGGCCCTTCGGCGCAGTATGTACTCCCACAAACTGAAACGACACCGCAccgagtt                                                                                                       >  2:7724049/1‑144 (MQ=255)
                                                  agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                    <  1:5936569/150‑1 (MQ=255)
                                                  agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAAACCAAACCCAAAGGGAGGGGGGGCCGGTCGGCGCTGTATGGACAACCAAACAAAGAAACGACACCCCCCACttttttt                                                                                                    >  2:536338/1‑150 (MQ=255)
                                                      ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                <  1:4334751/150‑1 (MQ=255)
                                                         ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCGCTCCCACAGGGCGAGGGGCCCCGTCGGCGCTGTTTGTTATACCGCACTCTGAAAAGACACCGCACGCTTTTTTTCTcccgc                                                                                             >  2:1285935/1‑148 (MQ=255)
                                                         ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAACCCACTCCCGCCGGGAGGGGGGACCCTTCGGCGCGGTATTTACTCCCACACACTGGAACCACACCCCCCACattttttaaaccac                                                                                             >  2:652714/1‑143 (MQ=255)
                                                          cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                            <  2:4095132/150‑1 (MQ=255)
                                                           tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACAACCAATCACACTCCCACCGGGGGCGGGGCCCCGTAGGCGCGGTTTGTTCGCCCACACACTGGAAAGACACCCCACACTTTTTTTATACCgcgc                                                                                           >  2:2661099/1‑150 (MQ=255)
                                                                  cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                    <  1:971786/150‑1 (MQ=255)
                                                                   gggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                   <  1:931522/149‑1 (MQ=255)
                                                                    ggCTCCCCTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                  <  2:5000103/150‑1 (MQ=255)
                                                                    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                  <  2:2589172/150‑1 (MQ=255)
                                                                    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                  <  2:8355534/150‑1 (MQ=255)
                                                                    ggCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                  <  2:5450981/150‑1 (MQ=255)
                                                                     gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCCCCCCCACACCCAAACCCAAAACCACAGGGAGGGGGGGCCCTTCCGCGCTGTATTTACTCCCACACACTGAAAACACAACCCACACTTTTTTTAACCCACGCCCCACCcggg                                                                                 >  2:2794773/1‑150 (MQ=255)
                                                                      cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                <  2:14059/150‑1 (MQ=255)
                                                                         ccATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAAACCGCACCCACAGGGGGTGGGGGCCCTTCGGCGCTGTATTTACTCCCACACACAGAAACGACACCGCACACTTTTTTTATCCCGCGCCCCACCCGGGcgag                                                                             >  2:1962885/1‑150 (MQ=255)
                                                                         ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                             <  1:6774600/150‑1 (MQ=255)
                                                                         ccATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCACAACCACACGGGGGGGGGGCCCGTAGGCGCGGTATGTTAACCCACACACAGAAAACAAACCCCCCAATTTTTTTTTACCCCCCCCCACCCGGGtgag                                                                             >  2:3757072/1‑150 (MQ=255)
                                                                           ctGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCTCACCCTCCCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                           <  1:6684003/149‑1 (MQ=255)
                                                                           aTGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCGAACCCAAAACCCAACGGTGTGGGGAACCGTCGGCGCAGGTTGTAACACCGCACGCAGAAACCACACCGCCAACATTTTTTATCCCGCCCCCCGCCCGGGCGgggg                                                                           >  2:1083766/1‑150 (MQ=255)
                                                                           aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                           <  2:2053929/150‑1 (MQ=255)
                                                                             ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc                                                                         <  2:4284974/150‑1 (MQ=255)
                                                                              gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAAACCACACCCAAAGGGCGGGGGGCACCGTAGGGGCGGTTTTGAAACCCGCACAATGTAACCACACCCCACACATTTTTTTTACCCCGCCCCCCCCGGGGGGGGGGCg                                                                        >  2:2548070/1‑150 (MQ=255)
                                                                               cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                       <  1:1258372/150‑1 (MQ=255)
                                                                                gAAAGGGAATGCGGCATAGTCGTTTGTGCCCCCGCACCCGAAACCACTACCTCAGGGAGAGGGGACCGTTCGGCGCAGTATTTACTCCCACAAACAGAAACGACAACGCACACATTTTTTATACCTCGCCCCTCCGGGGAGAGGGCCggg                                                                      >  2:8255165/1‑150 (MQ=255)
                                                                                gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                      <  2:4641815/150‑1 (MQ=255)
                                                                                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCCCTCCCCCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                  <  2:2947902/150‑1 (MQ=255)
                                                                                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                  <  1:6233396/150‑1 (MQ=255)
                                                                                     ggAATGCGGCATAGTCGTTTTCCCCCCCGCACCCTAACCCACTCCCACAGGGCGATGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACCACACCGCACACTTTTTTTCTCCCACGCCCCACCGGGGTGAGGGCCggggggtg                                                                 >  2:6275077/1‑146 (MQ=255)
                                                                                      gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                <  2:3419747/150‑1 (MQ=255)
                                                                                      gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                <  2:317388/150‑1 (MQ=255)
                                                                                      gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                <  2:6650677/150‑1 (MQ=255)
                                                                                      gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCGCCCTCGCCCCTCCGGGGAGATGGCCGGGGTGAgg                                                                <  1:4229927/150‑1 (MQ=255)
                                                                                       caTGCGGCCGAGTCGTGTGCGCCCCCGCCCCCGACCCCTCGCCCTCCGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCGCCCTCTGAAACGACCCCGCACTCTTTTTTTCGCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                               <  2:6819514/149‑1 (MQ=255)
                                                                                            ggCATTGTCGTTGGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGCGGGGACCGGTCGGGGCTGTTTGTACTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCCCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGaaaa                                                          <  1:216950/150‑1 (MQ=255)
                                                                                            ggCATAGTCGTTTGCGCCCCCACACCCGAAACCAAAACCTCCCGGAGAGGGGACCCGTCGGCGCGGTATTTACTACCCCACACAGAAACGACACCCCACGCTTTTTTTATGCCAAGCCCCACCCGGGAGAGGGCCGGGGGGAGGGGgaaa                                                          >  2:5323816/1‑150 (MQ=255)
                                                                                                   tCGTTTGCGCCCCCTCAACCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACACTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCa                                                   >  2:3545304/1‑150 (MQ=255)
                                                                                                           gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                           >  2:6543196/1‑150 (MQ=255)
                                                                                                           gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                           >  2:7475368/1‑150 (MQ=255)
                                                                                                                cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                      >  1:2763448/1‑150 (MQ=255)
                                                                                                                 tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                     >  1:7042338/1‑150 (MQ=255)
                                                                                                                 tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                     >  2:7229314/1‑150 (MQ=255)
                                                                                                                     ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTg                                 >  1:3286538/1‑150 (MQ=255)
                                                                                                                     ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                 >  1:5657313/1‑150 (MQ=255)
                                                                                                                     ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                 >  1:80344/1‑150 (MQ=255)
                                                                                                                        tccccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTACCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTATGCTa                              >  1:2266740/3‑150 (MQ=255)
                                                                                                                         aCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGCACAGGCCGCACTGCTCACCCAGGTTCTGCTAt                             >  2:7555700/1‑150 (MQ=255)
                                                                                                                          cccTCTCCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                            >  1:3109319/1‑150 (MQ=255)
                                                                                                                          cccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                            >  1:7736350/1‑150 (MQ=255)
                                                                                                                           ccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                           >  1:7711751/1‑150 (MQ=255)
                                                                                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                           >  1:39773/1‑150 (MQ=255)
                                                                                                                            ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                          >  2:60562/1‑150 (MQ=255)
                                                                                                                             tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                         >  1:4573425/1‑150 (MQ=255)
                                                                                                                                 ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                     >  1:1666160/1‑150 (MQ=255)
                                                                                                                                  cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                    >  1:66480/1‑150 (MQ=255)
                                                                                                                                  cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                    >  1:8284293/1‑150 (MQ=255)
                                                                                                                                  cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACACACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                   >  1:7329123/1‑150 (MQ=255)
                                                                                                                                   tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                   >  2:213895/1‑150 (MQ=255)
                                                                                                                                        gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa              >  2:8081761/1‑150 (MQ=255)
                                                                                                                                            ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATGTTCTGCTATCCTGCCCCCGCACTAAggg          >  2:5216443/1‑150 (MQ=255)
                                                                                                                                             gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg         >  2:643621/1‑150 (MQ=255)
                                                                                                                                             gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg         >  1:1980508/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAT  >  NC_000913/3780919‑3781216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: