Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,106 G→C 6.0% intergenic (+89/‑109) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1060GC 6.0% 1096.0 / 5.4 327intergenic (+89/‑109)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (192/107);  new base C (22/4);  total (216/111)
Fisher's exact test for biased strand distribution p-value = 5.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

GGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAA  >  NC_000913/3780957‑3781255
                                                                                                                                                      |                                                                                                                                                     
ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg                                                                                                                                                       <  2:2131703/150‑1 (MQ=255)
  ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                                     <  1:4933599/150‑1 (MQ=255)
  ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                                     <  2:7913608/150‑1 (MQ=255)
     tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACACACAACCACAGGGGGAGGGGGCCCGTCGGCGCTGTATGTACTCCCGCACACTGAAAAGACACCGcccgc                                                                                                                                                  >  1:2001497/1‑146 (MQ=255)
       ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAAACCACTCCCTCAGGGTGTGGGGACCGGTAGGGGGGGTTTGTACTGCCTCACTCTGAAACCACACCGcccaatt                                                                                                                                                >  2:5087825/1‑144 (MQ=255)
        gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                                                                                               <  2:3793109/150‑1 (MQ=255)
         tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTGGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                              <  1:1023524/150‑1 (MQ=255)
         tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                              <  1:5410831/150‑1 (MQ=255)
          aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                                             <  1:5909587/150‑1 (MQ=255)
            agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAAACCAAACCCAAAGGGAGGGGGGGCCGGTCGGCGCTGTATGGACAACCAAACAAAGAAACGACACCCCCCACttttttt                                                                                                                                           >  2:536338/1‑150 (MQ=255)
              agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                                         <  2:651038/150‑1 (MQ=255)
              agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                                         <  1:930693/150‑1 (MQ=255)
                ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                                       <  1:4334751/150‑1 (MQ=255)
                ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                                       <  2:5332119/150‑1 (MQ=255)
                   ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                                                    <  1:5040332/150‑1 (MQ=255)
                     tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCAACCAAAACCACAACCGCAGGGGGGGGGGGCCCGTCGGGGCGGGTTTTTATCCCACCCGCTGTAAACGCACCGCCCGCTTTTTTTTTccccccc                                                                                                                                  >  2:259623/1‑146 (MQ=255)
                      gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                                                                 <  1:6273602/150‑1 (MQ=255)
                         gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCACACCCGCAGGGAGCGGGGCCCCGTCGGCGCGGTGTGTACTCCCGCACACAGAAAAGACACCCCACACTATTTTTAACCCAccccccc                                                                                                                              >  2:4646052/1‑149 (MQ=255)
                          cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGTGCCCCCTCACCCTAACCCTCTACCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGCAAAGACACCGCCCTCTTTTTTTCTCCCTCGccccac                                                                                                                             >  2:7687820/1‑148 (MQ=255)
                            cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                                                           <  1:971680/150‑1 (MQ=255)
                              ggCTCCCCTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                         <  2:5000103/150‑1 (MQ=255)
                               gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCCCCCCCACACCCAAACCCAAAACCACAGGGAGGGGGGGCCCTTCCGCGCTGTATTTACTCCCACACACTGAAAACACAACCCACACTTTTTTTAACCCACGCCCCACCcggg                                                                                                                        >  2:2794773/1‑150 (MQ=255)
                                cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                                       <  1:830057/150‑1 (MQ=255)
                                  cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  1:270109/150‑1 (MQ=255)
                                   ccATGGCGAATGGGAATGGGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGATTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                    <  1:1825566/150‑1 (MQ=255)
                                   ccATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCACCCCAAACCACACCCACAGGGTGCGGGGCCCCTTGGGGGCGGTTTGTAAACCCGCACACTGTAACGACCCCGCCCACATTTTTTAAACCGCGCCCCCCCcggggggg                                                                                                                    >  2:485342/1‑146 (MQ=255)
                                   ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGGGCCCCCACACCCGAACCCACACCCAAAGGGAGAGGGGACCGGTCGGCGCGGTATGTACTACCACACTCTGAAACGAAACCGCACACATTTTTTATACCGCGCCCCACCGGGGggag                                                                                                                    >  2:5187648/1‑150 (MQ=255)
                                   ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCTCTACCACAGGGAGAGGGGACCCTTCGGCGCGGGAGGGACTCCCCCAAACTGAAACCACCCCGCCCACAATTGTTCTCCCTCGCCCCGCCcgggcgcg                                                                                                                    >  2:1082704/1‑146 (MQ=255)
                                    cATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAACCCCCCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                   <  2:3601494/150‑1 (MQ=255)
                                     aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                  <  1:6683754/150‑1 (MQ=255)
                                     aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                                  <  2:2053929/150‑1 (MQ=255)
                                     aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                  <  1:7484268/150‑1 (MQ=255)
                                         cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                              <  1:1258372/150‑1 (MQ=255)
                                          gAAAGGGAATGCGGCATAGTCGTTTGTGCCCCCGCACCCGAAACCACTACCTCAGGGAGAGGGGACCGTTCGGCGCAGTATTTACTCCCACAAACAGAAACGACAACGCACACATTTTTTATACCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                             >  2:8255165/1‑150 (MQ=255)
                                            aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                           <  1:5959875/150‑1 (MQ=255)
                                               ggAATGCGGCATAGTCGTTTTCCCCCCCGCACCCTAACCCACTCCCACAGGGCGATGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACCACACCGCACACTTTTTTTCTCCCACGCCCCACCGGGGTGAGGGCCggggggtg                                                                                                        >  2:6275077/1‑146 (MQ=255)
                                                gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                       <  2:4070041/150‑1 (MQ=255)
                                                gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                       <  2:6650677/150‑1 (MQ=255)
                                                gAATGCGGCATAGTCGTTTGCGCCCCCACCCCCCAACCCACACCCCCCCGGCGCGGGGACCCGGCCGCGCCGTAAGTACACCCACACACAGAAACGACACCCCACACATTTTTTCTCCCGCCCCCCACCCGGGAGAGGGCCGGGGGGAgg                                                                                                       >  2:522659/1‑150 (MQ=255)
                                                gAATGCGGATTCGTCGTTTGCGCCCCCTCACCCTAACCCGCTCCCTCTGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCGCCCTCGCCCCGCCGGGGAGAGGGCCGGGGTGAgg                                                                                                       <  1:3314425/150‑1 (MQ=255)
                                                 caTGCGGCCGAGTCGTGTGCGCCCCCGCCCCCGACCCCTCGCCCTCCGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCGCCCTCTGAAACGACCCCGCACTCTTTTTTTCGCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                      <  2:6819514/149‑1 (MQ=255)
                                                      ggCATAGTCGTTTGCGCCCCCCCACCCAAAACCACAACCACCGGGAGCGGGGACCCGTCGGCGCCGGTTGTACACCCGCACACAGAAACCACACCCCCCGCGTTTTTTAAACCGCGCCCCCCCCGGGGGCGGGCCGGGGGGAGGGGgaaa                                                                                                 >  2:4538954/1‑150 (MQ=255)
                                                      ggCAGAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGTGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                 <  2:7815787/150‑1 (MQ=255)
                                                             tCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGGATGTACGCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCa                                                                                          >  2:4268956/1‑150 (MQ=255)
                                                                     gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                  >  2:7475368/1‑150 (MQ=255)
                                                                     gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                  >  2:6543196/1‑150 (MQ=255)
                                                                          cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                                                             >  1:2468975/1‑150 (MQ=255)
                                                                           tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                                                            >  1:7042338/1‑150 (MQ=255)
                                                                           tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                                                            >  2:7229339/1‑150 (MQ=255)
                                                                              accTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGATCACCAATTTTCt                                                                         >  1:7721953/2‑150 (MQ=255)
                                                                               ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                        >  2:1581205/1‑150 (MQ=255)
                                                                               ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                        >  1:4527583/1‑150 (MQ=255)
                                                                               ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                        >  1:523807/1‑150 (MQ=255)
                                                                               ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                        >  1:5657313/1‑150 (MQ=255)
                                                                                cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                       >  2:2894383/1‑150 (MQ=255)
                                                                                 gtaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAATTTCTGCt                                                                      >  1:2285785/3‑150 (MQ=255)
                                                                                  aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCACTTTTCTGCTa                                                                     >  2:4398453/1‑150 (MQ=255)
                                                                                    cccTCTCCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                                   >  1:3109319/1‑150 (MQ=255)
                                                                                    cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                                   >  2:121487/1‑150 (MQ=255)
                                                                                     ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                  >  1:39773/1‑150 (MQ=255)
                                                                                      ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                 >  1:1354193/1‑150 (MQ=255)
                                                                                        ctcCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                               >  2:2991353/1‑150 (MQ=255)
                                                                                        ctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                               >  2:1742264/1‑150 (MQ=255)
                                                                                        ctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                               >  2:2655485/1‑150 (MQ=255)
                                                                                           ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                            >  1:7876326/1‑150 (MQ=255)
                                                                                                 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                      >  2:8352574/1‑150 (MQ=255)
                                                                                                  gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                     >  2:8195191/1‑150 (MQ=255)
                                                                                                  gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                     >  2:8081761/1‑150 (MQ=255)
                                                                                                  gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                     >  1:7781272/1‑150 (MQ=255)
                                                                                                       gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                >  2:643621/1‑150 (MQ=255)
                                                                                                        gcacggTTCGGCGTTTTTTGTACCCACCCCCTCCTAACCGCCACCCCCCTCTTTTTTCCCCCCGGGCCCGCCGGGGGTTGGGGCGGGGGTGGGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                               <  2:5552869/145‑1 (MQ=255)
                                                                                                         gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                              >  1:4652259/1‑150 (MQ=255)
                                                                                                          aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                             >  1:7273114/1‑150 (MQ=255)
                                                                                                              ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                         >  1:5535069/1‑150 (MQ=255)
                                                                                                                  gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGCCAAGGCCGCACTGCTCACCAATTTTCTGCTCTCCTGCCCCCGCAGTAAGGGGGCCGTATGCtc                                     >  2:6078489/1‑149 (MQ=255)
                                                                                                                   cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                    >  1:1574978/1‑150 (MQ=255)
                                                                                                                     cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGTGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                  >  2:1314730/1‑150 (MQ=255)
                                                                                                                     cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGACCCCGCACTAATGGGGCAGTATGCTAAAc                                  >  1:5194557/1‑150 (MQ=255)
                                                                                                                         atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                              >  2:5828794/1‑150 (MQ=255)
                                                                                                                           gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                            >  2:2570544/1‑150 (MQ=255)
                                                                                                                           gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                            >  1:2950093/1‑150 (MQ=255)
                                                                                                                              cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                         >  2:1442144/1‑150 (MQ=255)
                                                                                                                                cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                       >  2:1529004/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                      >  2:5436279/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                      >  1:669441/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                      >  2:3434219/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                      >  1:2234079/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCGCACCAAGTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCAGTCTGCGCAACAGCGGACTTGACg                     >  2:7564468/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                     >  2:6752448/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                     >  1:2185385/1‑150 (MQ=255)
                                                                                                                                      accctcaaaCGACCCCCCCATTTTTTTCCCCCCCCCCCCCCGCGGGGGTGGGGGGGGGGGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                  <  2:1997275/144‑1 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                >  2:25226/1‑150 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                >  1:4114756/1‑150 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                >  1:5477521/1‑150 (MQ=255)
                                                                                                                                            aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa            >  1:6730/1‑150 (MQ=255)
                                                                                                                                             aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt           >  2:3168770/1‑150 (MQ=255)
                                                                                                                                              aCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAtt          >  1:6482388/1‑150 (MQ=255)
                                                                                                                                                gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc        >  1:3816267/1‑150 (MQ=255)
                                                                                                                                                   acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc     >  1:8125799/1‑150 (MQ=255)
                                                                                                                                                     cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa   >  2:6039589/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAA  >  NC_000913/3780957‑3781255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: