Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,457 T→G 7.6% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4570TG 7.6% 1502.0 / 27.8 518intergenic (+12/+75)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (151/316);  new base G (49/2);  total (200/318)
Fisher's exact test for biased strand distribution p-value = 5.66e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 522 total aligned reads displayed.

GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCG  >  NC_000913/3959308‑3959605
                                                                                                                                                     |                                                                                                                                                    
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                    <  2:5634043/150‑1 (MQ=255)
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                    >  1:4441259/1‑150 (MQ=255)
   aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                   >  2:3439613/1‑150 (MQ=255)
     tCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCt                                                                                                                                                 <  2:2248432/150‑1 (MQ=255)
      ccGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTc                                                                                                                                                <  2:6845767/150‑1 (MQ=255)
       cGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCa                                                                                                                                               >  1:6624262/1‑150 (MQ=255)
       cGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCa                                                                                                                                               >  2:155544/1‑150 (MQ=255)
       cGGAAGGCGCGGTAGATAACGCGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGCTAGGTAAGAAACTGCGCGGCTATATGACAGATAAGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCa                                                                                                                                               >  2:3139432/1‑150 (MQ=255)
              cgcgGTAGTTTACGGGCATCTGCGTGATGTGAACGTAGCGATTCGGAGCCTTGCGATTGAGCAGGTAGGTAAGATACTGCGCGGCTATATGTCAGTTATGTAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCGTCAgccgtac                                                                                                                                        <  2:8165581/150‑5 (MQ=255)
               gcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAcc                                                                                                                                       <  2:6524153/150‑1 (MQ=255)
               gcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAcc                                                                                                                                       <  1:7257749/150‑1 (MQ=255)
                cgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAccc                                                                                                                                      >  1:4900563/1‑150 (MQ=255)
                   tAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCctct                                                                                                                                   <  2:6734946/150‑1 (MQ=255)
                    aGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCtctc                                                                                                                                  <  1:2720564/150‑1 (MQ=255)
                     gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                 <  2:7312695/150‑1 (MQ=255)
                     gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                 <  2:4128678/150‑1 (MQ=255)
                       tAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCca                                                                                                                               >  1:4151880/1‑150 (MQ=255)
                         aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                             <  1:8118988/150‑1 (MQ=255)
                         aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                             >  2:4811802/1‑150 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                            <  2:5216590/150‑1 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                            <  2:6113737/150‑1 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                            <  2:7717750/150‑1 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACAccccccg                                                                                                                            >  2:702048/1‑146 (MQ=255)
                           gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCTCACCCCGACCCTCTCCCACagg                                                                                                                           <  1:5027270/150‑1 (MQ=255)
                           gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                           <  1:6799861/150‑1 (MQ=255)
                             gCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCCACCCCGCCCCACTCCCCCacggg                                                                                                                         >  2:13588/1‑149 (MQ=255)
                              cAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggag                                                                                                                        <  2:4902017/150‑1 (MQ=255)
                                aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagag                                                                                                                      <  1:7879390/150‑1 (MQ=255)
                                   gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                   <  1:5462167/150‑1 (MQ=255)
                                   gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCACagggagaggga                                                                                                                   >  1:7544605/1‑150 (MQ=255)
                                   ccGTGAAGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGACACGTATTGCTGTTGCGGGTGAAGTGCGCGCTGATGCCCTCCCCCCCACCCACGCCCCCccggagcggga                                                                                                                   >  2:4463255/2‑150 (MQ=255)
                                      tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcgg                                                                                                                >  1:2140165/1‑146 (MQ=255)
                                        aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaac                                                                                                              <  2:2875334/150‑2 (MQ=255)
                                         tgtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGGGAGGGAGAAAac                                                                                                             >  2:8353238/1‑150 (MQ=255)
                                           tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                           >  2:4845289/1‑150 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATTTGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                         >  1:5190999/1‑150 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTCAGTGCGCGCTGATGCCCTCACCCCGACCCGCTCCCACAGGGCGAGGGTGAAAacacgc                                                                                                         >  1:1694755/1‑148 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                         <  1:2324551/150‑1 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACCCCCCGACCCTCTCCCACCGGGAGAGGGAGCAAacacgc                                                                                                         >  1:5832808/1‑148 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGAGGCCCGCACCCCGACCCGCGCCCACAGGGAGAGGGAGAAAacacgc                                                                                                         >  1:5939998/1‑148 (MQ=39)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCACACGGAGAGGGGGAAAAAACTCAAg                                                                                                      >  1:648214/1‑150 (MQ=39)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCCCCCCCCACCCACACCCCCACGGGGAGGGAGAAAACCCACaaa                                                                                                      >  1:7124216/1‑149 (MQ=255)
                                                  aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTATGAATCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTTCGTGCGCGCTGATGCCCTCACCCAGACCCTCTCCCACTGGGAGAGGGAGAATAGACTCaatga                                                                                                    <  2:1424716/150‑4 (MQ=38)
                                                      aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGATGGCCGCACCCCCGCCCGCGACCCCAGGGGGTGGGCGTAAAAACTCACTGCCTtc                                                                                                >  2:264333/1‑150 (MQ=255)
                                                          gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                            <  2:5520064/150‑1 (MQ=255)
                                                          gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                            <  2:8406351/150‑1 (MQ=255)
                                                           cAGCCATGCGATTGGGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                           <  1:5254442/150‑1 (MQ=255)
                                                           cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                           <  2:4024540/150‑1 (MQ=255)
                                                           cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACACCCCCCGGGGGAGGGAGAAAACACACAAGGCCATCTccgg                                                                                           >  2:6602522/1‑148 (MQ=255)
                                                              ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgag                                                                                        <  2:6426853/150‑1 (MQ=255)
                                                                aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                      <  1:1457651/150‑1 (MQ=255)
                                                                 tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAg                                                                                     <  1:3519404/150‑1 (MQ=255)
                                                                 tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                     >  1:6993733/1‑150 (MQ=255)
                                                                  gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                    <  1:7653220/150‑1 (MQ=255)
                                                                    gATTGAGCAGGTAGGTAAGAAACTGCGCGGATATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                  <  1:4755097/150‑1 (MQ=255)
                                                                     aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                 <  1:3412955/150‑1 (MQ=255)
                                                                     aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCCCCCCCCACCCACACCCCCACCCAGCGGGGGAAAAAACACAACGCCAGCACCAGGCGAAGGccc                                                                                 >  2:2336478/1‑149 (MQ=255)
                                                                      ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCtt                                                                                <  1:2230767/150‑1 (MQ=255)
                                                                        gAGCAGGTAGGTAAGAACCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTATGTGCGCGCTGATGCCCTCACCCCGCCCCTCTCCCACAGGGAGAGGGAGAAACCACTCAAGGCCTTCGCCAGGAGAAGGCCTTga                                                                              <  1:2869325/150‑2 (MQ=38)
                                                                         aGCAGTTAGGTTAGAAACTGCGCGGCTATTTGAGAGATATGAATCGTAGTGCTTTTGAGGGTTATGTGCGCTGTGATGGCCTAACACCGTCGCTCTCCCACAGGGAGAGGGAGAATACAATCAATGGCTTCTCCTGGATAAGGCTTTGCt                                                                             <  2:7849524/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                             <  1:1154775/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                             <  1:6332090/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                             <  1:7999862/150‑1 (MQ=255)
                                                                           cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                           <  1:1582470/150‑1 (MQ=255)
                                                                           cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                           <  2:1882416/150‑1 (MQ=255)
                                                                           cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                           <  2:7916372/150‑1 (MQ=255)
                                                                           cAGGTAGGTAAGAAACTGCGCGGCTATAGGACAGAAATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTAt                                                                           <  2:8057826/150‑1 (MQ=255)
                                                                            aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                          <  2:1982958/150‑1 (MQ=255)
                                                                             gggaggtaAGTAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACAGGGAGTGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                         <  2:4221921/147‑1 (MQ=255)
                                                                                 ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                     <  2:1609474/150‑1 (MQ=255)
                                                                                  gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                    <  1:3044136/150‑1 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCGCTCCCACCGGGGGTGGGAGAAAAAAAACAAGGCCATATCCTGGAGAAGGCCTTGCGATTAGTTTCgg                                                                  >  1:1744057/1‑150 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACACGGAGAGGGAGAAAACACACAAGGCCGTCAACAGGAGAAAGCCATGCAAATAGTTGCgg                                                                  >  1:8425552/1‑150 (MQ=255)
                                                                                       aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                               <  1:74051/150‑1 (MQ=255)
                                                                                       aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                               <  1:4264691/150‑1 (MQ=255)
                                                                                        aaCTGCGCCGCTATCTGACAGATATGAAACGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                              <  1:1040195/150‑1 (MQ=255)
                                                                                             cgcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc                                                         <  1:4477435/150‑1 (MQ=255)
                                                                                              gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACTCCCACACGGAGAGGGGGAAAACAATCAAAGCAGTCTCCAGGGGAAGGCCGTGCTATTATTTGCGGGAAAGCACCt                                                        >  1:1390939/1‑150 (MQ=37)
                                                                                                 gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                     <  2:5799625/150‑1 (MQ=255)
                                                                                                    atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTCACCCCGACCCAAACACCCCCGGAGAGGGGGAAAACACACAATGCCATATCCTGGAGAAGGCCTTTCGATTAGTTGCGGTACAGAACCATAattt                                                  >  2:7466001/1‑148 (MQ=255)
                                                                                                         aCAGATATGAAACGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                             <  1:6686657/150‑1 (MQ=255)
                                                                                                          cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCACAAGGCGAGGGGGAAAAAACAAAAGGCCATAACCAGGAGAAGGGCATGCGATTAGTTTCGGTTCAGCACCATAAAGAtgtgtt                                            >  1:5642458/1‑148 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGTGGGAGAAAACACTGAAGGCCTTCTCCTGGAGAAGGCCTTGGTATTAGTTGCGGTACAGCACCTTAATGATGTGata                                           >  2:6332682/1‑150 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACTCCCCCAGGGCGAGGGGGAAAACACTCAAGGCCTTATCCAGGAGAAGGCCATGCTATTAGTTTCGGTACAGCACCATAAAGATGTGtta                                           >  2:2696867/1‑150 (MQ=255)
                                                                                                               atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                                       <  2:3828170/150‑1 (MQ=255)
                                                                                                                  aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGGGAAAACACACAAGGCCTTATACAGGAGAATGCCGTGCTATTAGTTTCGGGACAGCACCATAATGTTTTGATATACGaaa                                    >  1:8184837/1‑149 (MQ=25)
                                                                                                                     cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                 <  2:2712736/150‑1 (MQ=255)
                                                                                                                     cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                 <  2:3324687/150‑1 (MQ=255)
                                                                                                                     cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                 <  1:7737006/150‑1 (MQ=255)
                                                                                                                        aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACAGGGCGAGGGAGAAAAAACACAAGGCCGTCTACGGGAGAAGGCCGTGCTATTATTTTCGGGACACCACCAAAAAGATGTTATAAACGAACAGGgtg                              >  2:7201652/1‑150 (MQ=255)
                                                                                                                           gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc                           <  2:5379902/150‑1 (MQ=255)
                                                                                                                                  cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                    <  2:365775/150‑1 (MQ=255)
                                                                                                                                     gTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg                 <  1:2531143/150‑1 (MQ=255)
                                                                                                                                         aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg             <  1:6137545/150‑1 (MQ=255)
                                                                                                                                           tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCt           <  2:1252324/150‑1 (MQ=255)
                                                                                                                                            gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc          <  1:3739629/150‑1 (MQ=255)
                                                                                                                                            gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc          <  2:247470/150‑1 (MQ=255)
                                                                                                                                                   gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTccc   >  2:5505591/1‑150 (MQ=255)
                                                                                                                                                   gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc   <  2:104457/150‑1 (MQ=255)
                                                                                                                                                    aTGCCCTCACACCGACCCTCTCCCACATGGAGAGGGAGAATACACTCAAGGCCTTCTCCTGGAGTAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCTGTCGGCTCCAGTACCg  <  1:4014290/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCG  >  NC_000913/3959308‑3959605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: