Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,099 T→C 68.7% W65R (TGG→CGG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,0990TC68.7% 131.7 / 85.1 96W65R (TGG→CGG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (16/14);  new base C (25/41);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCG  >  NC_000913/755952‑756244
                                                                                                                                                   |                                                                                                                                                 
ggTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCgg                                                                                                                                                 <  1:1053951/150‑1 (MQ=255)
   ggCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGaa                                                                                                                                              <  2:976186/150‑1 (MQ=255)
     cgcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATg                                                                                                                                            <  2:898781/150‑1 (MQ=255)
     cgcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATg                                                                                                                                            <  1:813612/150‑1 (MQ=255)
      gcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATgg                                                                                                                                           <  1:904460/150‑1 (MQ=255)
      gcAGGTATGCGCGCGGCGCTGCAAAGTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATgg                                                                                                                                           <  1:1028667/150‑1 (MQ=255)
             tGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGt                                                                                                                                    <  1:171197/150‑1 (MQ=255)
               cgcgcgGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTAc                                                                                                                                  <  2:832892/150‑1 (MQ=255)
                    ggcgCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGacac                                                                                                                             >  1:1155700/1‑150 (MQ=255)
                     gcgcTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACAcc                                                                                                                            <  1:1396641/150‑1 (MQ=255)
                     gcgcTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGGTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACAcc                                                                                                                            <  2:286204/150‑1 (MQ=255)
                       gcTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGt                                                                                                                          <  2:1040003/150‑1 (MQ=255)
                               tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGt                                                                                                                  <  2:195035/150‑1 (MQ=255)
                                   ccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                              >  1:623733/1‑150 (MQ=255)
                                   ccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                              <  1:643311/150‑1 (MQ=255)
                                     agagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGACAGGGTCGGACt                                                                                                            >  2:192328/1‑150 (MQ=255)
                                      gagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACta                                                                                                           >  1:1162045/1‑150 (MQ=255)
                                       agCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACtat                                                                                                          <  2:68082/150‑1 (MQ=255)
                                          ggCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                                                                       <  1:1287636/150‑1 (MQ=255)
                                           gCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCg                                                                                                      <  1:613305/150‑1 (MQ=255)
                                            ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCgg                                                                                                     >  2:773906/1‑150 (MQ=255)
                                             cAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGt                                                                                                    >  2:1090787/1‑150 (MQ=255)
                                             cAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGt                                                                                                    >  2:1090778/1‑150 (MQ=255)
                                             cAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGt                                                                                                    <  2:677126/150‑1 (MQ=255)
                                             cAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGt                                                                                                    <  2:613151/150‑1 (MQ=255)
                                             cAGACCTGTGAGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGt                                                                                                    >  2:1090765/1‑150 (MQ=255)
                                                aCCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAc                                                                                                 <  1:883028/150‑1 (MQ=255)
                                                        gctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcgc                                                                                         >  1:240190/1‑150 (MQ=255)
                                                         ctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcgcg                                                                                        >  2:1364399/1‑150 (MQ=255)
                                                          tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                       >  2:1202773/1‑150 (MQ=255)
                                                          tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                       >  2:1202774/1‑150 (MQ=255)
                                                          tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                       >  2:859956/1‑150 (MQ=255)
                                                          tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                       >  2:631685/1‑150 (MQ=255)
                                                          tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                       >  1:24373/1‑150 (MQ=255)
                                                            ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                                                                     <  1:874738/150‑1 (MQ=255)
                                                             tctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTg                                                                                    <  1:481387/150‑1 (MQ=255)
                                                               tctACAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  <  2:923504/150‑1 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  <  2:1347118/150‑1 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  <  2:1358077/150‑1 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  <  2:1358079/150‑1 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  >  2:1162075/1‑150 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                                                                  >  2:252925/1‑150 (MQ=255)
                                                               tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATAGaa                                                                                  >  2:459270/1‑150 (MQ=255)
                                                                 tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata                                                                                >  1:628293/1‑150 (MQ=255)
                                                                 tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata                                                                                >  1:741478/1‑150 (MQ=255)
                                                                   aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatata                                                                              <  1:113424/150‑1 (MQ=255)
                                                                    aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatatat                                                                             <  1:779941/150‑1 (MQ=255)
                                                                    aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatatat                                                                             <  2:445726/150‑1 (MQ=255)
                                                                    aGTCGTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatatat                                                                             <  1:779944/150‑1 (MQ=255)
                                                                      tCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgt                                                                           <  1:539277/150‑1 (MQ=255)
                                                                        ttCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGGGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATgtgt                                                                         >  1:101301/1‑150 (MQ=255)
                                                                        ttCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATgtgt                                                                         >  2:1140882/1‑150 (MQ=255)
                                                                             gACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAAc                                                                    >  1:554642/1‑150 (MQ=255)
                                                                             gACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAAc                                                                    <  2:1036402/150‑1 (MQ=255)
                                                                                   ttCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              <  1:821621/150‑1 (MQ=255)
                                                                                   ttCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              <  1:884367/150‑1 (MQ=255)
                                                                                   ttCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              <  1:821569/150‑1 (MQ=255)
                                                                                   ttCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              >  1:601215/1‑150 (MQ=255)
                                                                                   ttCCCATACCATTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              <  2:1308894/150‑1 (MQ=255)
                                                                                   ttCCCATACCATTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                              <  2:1089816/150‑1 (MQ=255)
                                                                                       cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                          <  2:145802/150‑1 (MQ=255)
                                                                                        aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg                                                         <  1:739533/150‑1 (MQ=255)
                                                                                        aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg                                                         >  2:242026/1‑150 (MQ=255)
                                                                                            cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCAAGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt                                                     >  2:1154319/1‑150 (MQ=255)
                                                                                             gTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                    >  1:241645/1‑150 (MQ=255)
                                                                                              tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTc                                                   <  2:289958/150‑1 (MQ=255)
                                                                                              tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTc                                                   >  2:1188838/1‑150 (MQ=255)
                                                                                               ttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt                                                  <  2:173576/150‑1 (MQ=255)
                                                                                               ttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt                                                  <  2:458289/150‑1 (MQ=255)
                                                                                                 cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTgg                                                <  2:1359018/150‑1 (MQ=255)
                                                                                                  tGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAACCCGGGCCGGAAGCGATTCTGGa                                               <  2:206506/150‑1 (MQ=255)
                                                                                                  tGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGa                                               >  2:3753/1‑150 (MQ=255)
                                                                                                   gcgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGGGACAGGGTCGGACTATATCGGTGACCAGGCCGCGAGTGAAGATATGTGTAAAACCGGGCCGGAAGCGAGTCTGGac                                              >  2:1317218/1‑149 (MQ=255)
                                                                                                     gcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACt                                            >  1:98272/1‑150 (MQ=255)
                                                                                                         ggcggcATTACCGTTGCGCTGGGTAATACCCATGAATATAACCGGGAATGGCATATGTACGACACCGTGAATGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATTTGTGTAAAACGGGGCCGGAAGCGATTCTGGAACTCGaa                                        <  1:1255921/150‑1 (MQ=255)
                                                                                                          gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                       >  2:302619/1‑150 (MQ=255)
                                                                                                          gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                       >  1:557439/1‑150 (MQ=255)
                                                                                                             gcATTAGCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTCCGACACCGTGAATGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAAcaca                                    <  2:281536/150‑1 (MQ=255)
                                                                                                             gcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAAcaca                                    <  2:261084/150‑1 (MQ=255)
                                                                                                              cATTACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACAt                                   >  1:406001/1‑150 (MQ=255)
                                                                                                                ttACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                 >  1:713776/1‑150 (MQ=255)
                                                                                                                ttACCGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                 <  2:24373/150‑1 (MQ=255)
                                                                                                                    cGTTGCGCTGGGTACTACCCATGAAGATAACCGGGAATGGCATAGGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAATACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt                             <  2:1364471/150‑1 (MQ=255)
                                                                                                                    cGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt                             <  2:965851/150‑1 (MQ=255)
                                                                                                                    cGTTGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGCTGACCAGGACGCGAGTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt                             >  2:12966/1‑150 (MQ=255)
                                                                                                                      ttGCGCTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                           >  1:136586/1‑150 (MQ=255)
                                                                                                                          gcTGGGTAATACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGtt                       >  1:1113335/1‑150 (MQ=255)
                                                                                                                           cTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc                      >  1:755839/1‑150 (MQ=255)
                                                                                                                                   tACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtct              <  2:23643/150‑1 (MQ=255)
                                                                                                                                   tACCCATGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtct              <  2:463656/150‑1 (MQ=255)
                                                                                                                                        aTGAAGATAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatg         >  2:581228/1‑150 (MQ=255)
                                                                                                                                           aaGATTTCTGGGAATGGCATATGTACGACACCGTGACAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAAGATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg      <  2:1155495/150‑1 (MQ=255)
                                                                                                                                             gATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt    >  1:1308213/1‑150 (MQ=255)
                                                                                                                                              aTAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc   <  1:819557/150‑1 (MQ=255)
                                                                                                                                              aTAACCGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc   <  2:1370882/150‑1 (MQ=255)
                                                                                                                                               tAACCGGGATTGGCATATGTACGACACCGTGAATGGGTCTGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATTGTCg  <  2:930648/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCG  >  NC_000913/755952‑756244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: