Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,309 A→T 6.5% Q464L (CAG→CTG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,3090AT 6.5% 309.7 / 4.5 99Q464L (CAG→CTG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (49/38);  new base T (3/6);  total (52/47)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTATTGTGATT  >  NC_000913/1708163‑1708457
                                                                                                                                                   |                                                                                                                                                    
ttGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGc                                                                                                                                                   >  1:59590/1‑150 (MQ=255)
   cATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGt                                                                                                                                               <  1:347267/150‑1 (MQ=255)
    aTTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGt                                                                                                                                               <  1:1109663/150‑1 (MQ=255)
    aTTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGt                                                                                                                                               >  2:1077163/1‑150 (MQ=255)
     ttGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTc                                                                                                                                              <  2:321706/150‑1 (MQ=255)
     ttGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTc                                                                                                                                              <  2:321637/150‑1 (MQ=255)
     ttGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTc                                                                                                                                              >  2:773091/1‑150 (MQ=255)
       gAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCCATCTTCCCCTGGTGCACTATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCCGGAAGAAAAGCGCGCCGCAGACGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg                                                                                                                                            <  2:1400763/150‑1 (MQ=255)
         aTGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGa                                                                                                                                          <  1:1264487/150‑1 (MQ=255)
            tgGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAgcg                                                                                                                                       >  1:396772/1‑150 (MQ=255)
            tgGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAgcg                                                                                                                                       >  1:5772/1‑150 (MQ=255)
            tgGGGCTTGCGCGTGGGTTTGCCCGAGCAATAGTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAgcg                                                                                                                                       <  1:507756/150‑1 (MQ=255)
            tgGGGCGTGCGCGTGGGTTTGCCCGAGCAATAGTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCCGGAgcg                                                                                                                                       <  1:507797/150‑1 (MQ=255)
              gggCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGcgcg                                                                                                                                     >  1:1351794/1‑150 (MQ=255)
                  ttGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGaaaa                                                                                                                                 >  1:737354/1‑150 (MQ=255)
                    gcgcGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAg                                                                                                                               <  1:530503/150‑1 (MQ=255)
                    gcgcGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAg                                                                                                                               <  2:1023446/150‑1 (MQ=255)
                      gcgTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCg                                                                                                                             >  2:1300308/1‑150 (MQ=255)
                      gcgTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCg                                                                                                                             >  1:512799/1‑150 (MQ=255)
                             tttGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAATGCTGAAATTGCGGCTATTCGTCAGTAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGcc                                                                                                                      <  2:1102047/150‑1 (MQ=255)
                             tttGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGcc                                                                                                                      >  1:224676/1‑150 (MQ=255)
                                  ccGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGaa                                                                                                                 >  2:691600/1‑150 (MQ=255)
                                  ccGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGaa                                                                                                                 >  2:165242/1‑150 (MQ=255)
                                  ccGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGaa                                                                                                                 >  2:439272/1‑150 (MQ=255)
                                       cAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGACAAAGCGGCTCGCCTTGAACGACa                                                                                                            >  1:505573/1‑150 (MQ=255)
                                       cAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACa                                                                                                            >  1:1044420/1‑150 (MQ=255)
                                        aaTATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGACGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACAt                                                                                                           <  2:441091/150‑1 (MQ=255)
                                            ttCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAaga                                                                                                       <  2:591794/150‑1 (MQ=255)
                                                ccTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAgcgc                                                                                                   >  2:879346/1‑150 (MQ=255)
                                                ccTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAgcgc                                                                                                   >  1:315610/1‑150 (MQ=255)
                                                        aaTATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTc                                                                                           >  2:634479/1‑150 (MQ=255)
                                                           ttttCCGTCAGTAAAAAGCTGAATGTGGGGCTTTTCGTCAGTAATAAAAGCGCGCCGCTTAAGCAAAAGCGCGTTTCTACGCGCGCCTGGCTCGTCTGGCGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAAc                                                                                        <  1:795799/149‑1 (MQ=255)
                                                           ttttCCGTAAGGAAAATGCTGAAATTGGGGATTTTCGTCTGGAATAAAAGCGCGCCGCAGAAGCCAAAGCGGTTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAAc                                                                                        <  1:795870/149‑1 (MQ=255)
                                                           atTTCCGTCAGTAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAAc                                                                                        <  2:1224856/150‑1 (MQ=255)
                                                           atTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAAc                                                                                        <  2:403754/150‑1 (MQ=255)
                                                               ccGTCAGTAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGc                                                                                    <  2:601272/150‑1 (MQ=255)
                                                               ccGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGc                                                                                    >  2:1338016/1‑150 (MQ=255)
                                                                cttccgcaaaacGCTTAATTTGGGGATATTCGTCAGTAAGAAAAGCGCGCCGCAGACGCCACCGCGCTTTGCGAAGCGCGCCTGGCTCGTCTGGCGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCa                                                                                   <  1:430290/143‑1 (MQ=255)
                                                                cGTAAGTAAAATGCTTAATTTGCGGATATTCGTCAGTAATAAACGCGCGCCGAAGAACCCAACGCGCTTTTCTACGCGCGCCTGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCa                                                                                   <  1:1060645/150‑1 (MQ=255)
                                                                 gtaagtaaaacGCTTCAATTGCGGCTTTTCGTCAGTACTAAACGCGCGCCGCAGACCCAAAAGCGCGTTTCGAAGCGCGCCTGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTTAACGACATAAGAGCGCAGCCGTTCAACCTGCAg                                                                                  <  1:1300316/144‑1 (MQ=255)
                                                                 gTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAg                                                                                  >  1:775262/1‑150 (MQ=255)
                                                                 gTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAg                                                                                  >  1:775270/1‑150 (MQ=255)
                                                                     ggAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGTGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaa                                                                              >  2:277885/1‑150 (MQ=255)
                                                                      gAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGGGCCAGGCTCGAGTCGTCCCCGAAAAAGCGGCTCGCCTTGAACGACATTACACCGCAGCCGTTCAACCTGCAGCCaaa                                                                             <  2:1227199/150‑1 (MQ=255)
                                                                       aaaaaGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaag                                                                            >  2:531685/1‑150 (MQ=255)
                                                                         aaaGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagat                                                                          <  2:1106062/150‑1 (MQ=255)
                                                                           aGCTTAAATTGCGGCTTTTCGTCAGGAAGAAACGCGCGCCGCAGAAGCCAAAGCGCGTTTCGACGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataa                                                                        <  1:781228/150‑1 (MQ=255)
                                                                           aGCTGAAATTGCGGCTATTCGTCAGTAAGAAAAGCGCGCCGCAGACGCCAACGCGCGTTTCGAAGCGCGCCTGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataa                                                                        <  1:781220/150‑1 (MQ=255)
                                                                           aGCTGAAATTGCGGCTATTCGTCAGTAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCCGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataa                                                                        <  1:781259/150‑1 (MQ=255)
                                                                              tGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataaaga                                                                     >  1:41446/1‑150 (MQ=255)
                                                                               taaaTTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCATAAGCAAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGCGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataaagat                                                                    <  1:260558/149‑1 (MQ=255)
                                                                               gAATTTGCGGATATTCGTCAGTAATAAAAGCGCGCCGCAGAAGCAAACGCGGGTTTCGAAGCGCGCCCGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCaaagataaagat                                                                    <  1:260590/150‑1 (MQ=255)
                                                                                aaaTTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATg                                                                   >  2:872754/1‑150 (MQ=255)
                                                                                  aTTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCg                                                                 >  1:693554/1‑150 (MQ=255)
                                                                                  aTTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTATCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCg                                                                 >  1:693680/1‑150 (MQ=255)
                                                                                     gCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCTCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGAtt                                                              >  1:315938/1‑150 (MQ=255)
                                                                                     gCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGAtt                                                              >  1:1250747/1‑150 (MQ=255)
                                                                                      cGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATtg                                                             >  2:785853/1‑150 (MQ=255)
                                                                                      cGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATtg                                                             >  2:1048200/1‑150 (MQ=255)
                                                                                      cGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATtg                                                             >  2:73324/1‑150 (MQ=255)
                                                                                             tCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGc                                                      >  1:674226/1‑150 (MQ=255)
                                                                                                tCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTg                                                  >  1:763638/1‑150 (MQ=255)
                                                                                                   ggAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGgc                                                <  1:1035235/150‑1 (MQ=255)
                                                                                                      agacacgcgcgcCGCAGACGCCACCGCGCGTTGCGACGCGCGCCCGGCTCGTCGGGAGCGCGACAAAGCGGCTCGCCTTGAACGACATACGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGcgcg                                             <  2:1066098/144‑1 (MQ=255)
                                                                                                      agaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGcgcg                                             <  2:59590/150‑1 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            >  2:1139397/1‑150 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            <  2:1232682/150‑1 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            <  1:101174/150‑1 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            >  2:1139388/1‑150 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            >  2:1318635/1‑150 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            >  2:1004623/1‑150 (MQ=255)
                                                                                                       gaaAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCgg                                            <  1:939225/150‑1 (MQ=255)
                                                                                                             cgcgcCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGaaa                                      <  2:215351/150‑1 (MQ=255)
                                                                                                               cgccgcAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAaga                                    <  2:86317/150‑1 (MQ=255)
                                                                                                               cgccgcAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAaga                                    <  1:1125084/150‑1 (MQ=255)
                                                                                                               cgccgcAGAAGCCAAAGCGCGTGTCGACGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGCCCGAAGAGCGCCGCCGTGCAACCTGCAGCCCACGATAAAGATGCGAGTGCGGCCGCTCTGGCGCGGGTGACaga                                    >  2:57438/1‑150 (MQ=255)
                                                                                                                gccgcAGAAGCCAACGCGCGTTTCGACGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAagag                                   <  2:1299128/150‑1 (MQ=255)
                                                                                                                gccgcAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAagag                                   <  1:879346/150‑1 (MQ=255)
                                                                                                                gccgcAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAagag                                   <  1:1143664/150‑1 (MQ=255)
                                                                                                                    cAGAAGCCAAAGCTCGTTTCTAAGCTCGCCTGGATCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACTACATAAGAGCTCAGCCGTTCAACCTTCAGACAAATATAAATATGCGATCGCTGCCTCTCTGGCGCGGGTGAAAGAGAAAc                               <  2:1213872/150‑1 (MQ=255)
                                                                                                                    cAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAAc                               <  2:1213876/150‑1 (MQ=255)
                                                                                                                         gCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGcc                          >  2:197935/1‑150 (MQ=255)
                                                                                                                          gcctggcgcgcTTCGAAACGCGCTTTGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGcc                          >  1:466810/6‑150 (MQ=255)
                                                                                                                          ccAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCCCAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGACAGAGAAACAGGccc                         >  1:621043/1‑150 (MQ=255)
                                                                                                                           cAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCa                        >  2:922206/1‑150 (MQ=255)
                                                                                                                           cAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCa                        >  2:922286/1‑150 (MQ=255)
                                                                                                                            aaaGCGCGTTTCGAAGCGCGCCAGGCTCGACTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAg                       <  1:1250297/150‑1 (MQ=255)
                                                                                                                             aaGCGCGTTTCGAAGCGCGCCATGCTCGTCTGGAGAGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGAAGCCGTTCAACCTGCAGCCAAAGATAAATATGCGATAGCTGCCGCTCTGGCGCGGGAGAAAGAGAAACAGGACCAgg                      >  2:1225910/1‑150 (MQ=255)
                                                                                                                             aaGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAgg                      >  1:929922/1‑150 (MQ=255)
                                                                                                                             aaGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAgg                      >  1:929904/1‑150 (MQ=255)
                                                                                                                               gcgcgcTTCGAAACGCGCTTTGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGc                     >  1:1140725/1‑150 (MQ=255)
                                                                                                                               gcgcgcTTCGAAACGCGCTTTGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGc                     >  1:1140704/1‑150 (MQ=255)
                                                                                                                                  cgTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTaca                 <  2:895713/150‑1 (MQ=255)
                                                                                                                                   gTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTCCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTacac                >  2:832637/1‑150 (MQ=255)
                                                                                                                                           gcgcgcCAGGCTCGTCTGGAGCGCTAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTAtt        <  1:479746/150‑1 (MQ=255)
                                                                                                                                           gcgcgcCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTAtt        <  1:479726/150‑1 (MQ=255)
                                                                                                                                            cgcgcCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTATtg       >  2:439277/1‑150 (MQ=255)
                                                                                                                                               gcCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTATTGTGa    <  1:1196911/150‑1 (MQ=255)
                                                                                                                                                  cAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTATTGTGAtt  >  1:728824/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGGAGCGCGAAAAAGCGGCTCGCCTTGAACGACATAAGAGCGCAGCCGTTCAACCTGCAGCCAAAGATAAAGATGCGATTGCTGCCGCTCTGGCGCGGGTGAAAGAGAAACAGGCCCAGGCTACACAGCCTATTGTGATT  >  NC_000913/1708163‑1708457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: