Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 152,311 T→C 5.6% T168A (ACA→GCA)  yadM ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913152,3110TC 5.6% 243.3 / 3.9 73T168A (ACA→GCA) yadMputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (31/37);  new base C (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCG  >  NC_000913/152162‑152460
                                                                                                                                                     |                                                                                                                                                     
cGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGt                                                                                                                                                       >  1:1008118/1‑150 (MQ=255)
   tGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTc                                                                                                                                                    >  1:707671/1‑150 (MQ=255)
     aaCAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCAc                                                                                                                                                  <  2:33177/150‑1 (MQ=255)
         aTGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAg                                                                                                                                              <  2:412675/150‑1 (MQ=255)
          tGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAgc                                                                                                                                             >  1:913868/1‑150 (MQ=255)
              gCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCac                                                                                                                                         >  1:1170824/1‑149 (MQ=255)
              gCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCaa                                                                                                                                         >  2:370086/1‑150 (MQ=255)
              gCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGCTTCACGTAGCGCaa                                                                                                                                         >  1:63745/1‑150 (MQ=255)
              gCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGCTTCACGTAGCGCaa                                                                                                                                         >  1:63814/1‑150 (MQ=255)
                  ccAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGt                                                                                                                                     >  1:841459/1‑150 (MQ=255)
                    aaaaCGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCa                                                                                                                                   >  1:637871/1‑150 (MQ=255)
                     aaaCGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCAt                                                                                                                                  >  2:314941/1‑150 (MQ=255)
                     aaaCGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCAt                                                                                                                                  >  2:314947/1‑150 (MQ=255)
                         gACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCa                                                                                                                              >  2:256181/1‑150 (MQ=255)
                         gACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCa                                                                                                                              >  1:354046/1‑150 (MQ=255)
                         gACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCa                                                                                                                              >  1:354048/1‑150 (MQ=255)
                          acacTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCaa                                                                                                                             <  1:695932/150‑1 (MQ=255)
                          acacTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCaa                                                                                                                             <  1:695945/150‑1 (MQ=255)
                            acTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGa                                                                                                                           >  2:913954/1‑150 (MQ=255)
                                ctgGATAATCCATAACGTAAAATTTTAAAAGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATACCCCGGTACGCCTTGCCCTGCATCTGATTCACGTAGCGCAACGGTCATTTCAAGACCCt                                                                                                                       >  2:424581/1‑150 (MQ=255)
                                       aTCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCggg                                                                                                                <  2:961169/150‑1 (MQ=255)
                                       aTCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCggg                                                                                                                <  1:644224/150‑1 (MQ=255)
                                         ccATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCt                                                                                                              >  1:1133886/1‑150 (MQ=255)
                                         ccATAACGTAAATTTTTAAATGCCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCt                                                                                                              <  2:10395/150‑1 (MQ=255)
                                           aTAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGc                                                                                                            >  2:1283063/1‑150 (MQ=255)
                                                   aaTTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTc                                                                                                    <  1:588526/150‑1 (MQ=255)
                                                   aaTTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTc                                                                                                    <  1:823421/150‑1 (MQ=255)
                                                            aTGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCa                                                                                           <  1:1087257/150‑1 (MQ=255)
                                                            aTGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCa                                                                                           <  1:258245/150‑1 (MQ=255)
                                                                   catAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATc                                                                                    >  1:763095/1‑150 (MQ=255)
                                                                      aaTTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCttt                                                                                 >  1:1266821/1‑150 (MQ=255)
                                                                           tCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCACGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGc                                                                            >  2:893981/1‑150 (MQ=255)
                                                                             cttcttATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCAc                                                                          <  1:983790/150‑1 (MQ=255)
                                                                                  tATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTa                                                                     <  2:216452/150‑1 (MQ=255)
                                                                                   aTTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAg                                                                    <  1:754490/150‑1 (MQ=255)
                                                                                   aTTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAg                                                                    <  1:754670/150‑1 (MQ=255)
                                                                                    ttGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAgg                                                                   >  1:344004/1‑150 (MQ=255)
                                                                                    ttGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAgg                                                                   >  2:32295/1‑150 (MQ=255)
                                                                                      gATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGtt                                                                 >  2:955268/1‑150 (MQ=255)
                                                                                         aGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTaa                                                              <  1:350795/150‑1 (MQ=255)
                                                                                         aGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTaa                                                              <  1:1300101/150‑1 (MQ=255)
                                                                                            tGAAATTAAACGTCGCCAGTGCACGACAATTCCCCGGTACGCCTTGCCCTGCAACTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGa                                                           <  2:734101/150‑1 (MQ=255)
                                                                                            tGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGa                                                           >  2:189772/1‑150 (MQ=255)
                                                                                            tGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGa                                                           >  2:189771/1‑150 (MQ=255)
                                                                                             gAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGaa                                                          <  2:1333114/150‑1 (MQ=255)
                                                                                             gAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGaa                                                          <  2:1333180/150‑1 (MQ=255)
                                                                                              aaaTTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAgc                                                                                                                                             <  1:14198/66‑1 (MQ=255)
                                                                                              aaaTTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAgc                                                                                                                                             >  2:14198/1‑66 (MQ=255)
                                                                                               aaTTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAg                                                        <  1:269539/150‑1 (MQ=255)
                                                                                                 ttAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTg                                                      >  2:993226/1‑150 (MQ=255)
                                                                                                    aaCGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGca                                                   <  2:187996/150‑1 (MQ=255)
                                                                                                             aGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagta                                          <  1:1305321/150‑1 (MQ=255)
                                                                                                             aGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagta                                          <  1:734220/150‑1 (MQ=255)
                                                                                                              gTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCagtag                                         <  2:903806/150‑1 (MQ=255)
                                                                                                                    gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtt                                   <  2:1307703/150‑1 (MQ=255)
                                                                                                                    gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGCTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtt                                   <  2:63814/150‑1 (MQ=255)
                                                                                                                    gAAAATTCCCCGGTACGCCTTGCCCTGCATCTGCTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtt                                   <  2:63745/150‑1 (MQ=255)
                                                                                                                        aTTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtttttt                               <  1:492459/150‑1 (MQ=255)
                                                                                                                        aTTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtttttt                               <  2:1008118/150‑1 (MQ=255)
                                                                                                                        aTTCCCCGGGACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGtttttt                               <  2:1216876/150‑1 (MQ=255)
                                                                                                                          tCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGa                             >  1:348964/1‑150 (MQ=255)
                                                                                                                            cccGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGaaa                           <  2:1222485/150‑1 (MQ=255)
                                                                                                                              cGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAAcc                         >  1:1258826/1‑150 (MQ=255)
                                                                                                                                  aCGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATa                     <  2:1164134/150‑1 (MQ=255)
                                                                                                                                     ccTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCg                  >  2:1349506/1‑150 (MQ=255)
                                                                                                                                     ccTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCg                  >  2:805980/1‑150 (MQ=255)
                                                                                                                                     ccTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTGTTTTGAAACCCATACCg                  >  2:805985/1‑150 (MQ=255)
                                                                                                                                         gCCCTGCATCTGTGTCACGTAGCGCATCGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGatat              <  1:3600/150‑1 (MQ=255)
                                                                                                                                           ccTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTa            <  1:994901/150‑1 (MQ=255)
                                                                                                                                              gCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACtt         <  2:1017463/150‑1 (MQ=255)
                                                                                                                                               cATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTg        <  1:700735/150‑1 (MQ=255)
                                                                                                                                                  cTGTTTCAAGAAGCGCAAAGGACAAATCAAGACCCTTATCGTGCTGAAACTCTAAAGTGCTCAAGCCTATCTGTTCCGCACTGTTAGGAATAATTAAAGTGGCATCATCAGTAGTCCGATTTTTGAAAACCATACCGATATTAATTgtcg     >  2:313803/1‑150 (MQ=255)
                                                                                                                                                   tGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTgtcgt    >  1:303123/1‑150 (MQ=255)
                                                                                                                                                   tGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTgtcgt    <  2:1229672/150‑1 (MQ=255)
                                                                                                                                                     tttCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGtcgtcg  >  2:748654/1‑150 (MQ=255)
                                                                                                                                                     tttCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGtcgtcg  >  1:330107/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTGAACAATGAGGCAGCCAAAACGACACTGCTGGATAATCCATAACGTAAATTTTTAAATGTCATCATAATTATCCTTCTTATTGATAGATGAAATTAAACGTCGCCAGTGCACGAAAATTCCCCGGTACGCCTTGCCCTGCATCTGTTTCACGTAGCGCAACGGTCATTTCAAGACCCTTATCGGGCTGCATCTCGTCTGTGCTCCAGCGTATCTTTTCCGCACTGTTAGGTTTAAGGAAAGTGGCATCATCAGTAGTCCGTTTTTTGAAACCCATACCGATATTACTTGTCGTCG  >  NC_000913/152162‑152460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: