Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,163,148 C→T 6.4% G112S (GGC→AGC)  plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,163,1480CT 6.4% 312.3 / 8.7 93G112S (GGC→AGC) plsC1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (43/44);  new base T (3/3);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AGTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCC  >  NC_000913/3162999‑3163289
                                                                                                                                                     |                                                                                                                                             
aGTCTTGAACGGTAGCAGGCCGCGACCACGTCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGcc                                                                                                                                               <  1:713913/150‑1 (MQ=255)
aGTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGcc                                                                                                                                               <  2:599643/150‑1 (MQ=255)
 gTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCg                                                                                                                                              <  1:519948/150‑1 (MQ=255)
 gTCTTGAACGGGAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCg                                                                                                                                              <  2:917026/150‑1 (MQ=255)
    ttGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCTGGtt                                                                                                                                           >  1:233918/1‑150 (MQ=255)
    ttGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCTGGtt                                                                                                                                           >  1:994530/1‑150 (MQ=255)
    ttGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCTGGtt                                                                                                                                           >  1:233995/1‑150 (MQ=255)
     tGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTa                                                                                                                                          <  1:1074236/150‑1 (MQ=255)
       aaCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc                                                                                                                                        >  1:138577/1‑150 (MQ=255)
       aaCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc                                                                                                                                        >  1:138580/1‑150 (MQ=255)
       aaCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc                                                                                                                                        >  2:1127444/1‑150 (MQ=255)
       aaCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc                                                                                                                                        <  1:1039261/150‑1 (MQ=255)
       aaCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc                                                                                                                                        <  1:839096/150‑1 (MQ=255)
        aCGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAcc                                                                                                                                       >  1:790492/1‑150 (MQ=255)
           gTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGt                                                                                                                                    >  2:313639/1‑150 (MQ=255)
            tAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTa                                                                                                                                   >  2:850289/1‑150 (MQ=255)
            tAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTa                                                                                                                                   >  2:850291/1‑150 (MQ=255)
              gCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACa                                                                                                                                 >  1:1042056/1‑150 (MQ=255)
                   ccGCGACCTCGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGc                                                                                                                            <  2:1110832/150‑1 (MQ=255)
                   ccGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGc                                                                                                                            <  2:1110805/150‑1 (MQ=255)
                   ccGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGc                                                                                                                            <  2:1110830/150‑1 (MQ=255)
                    cgcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGcc                                                                                                                           >  1:1217925/1‑150 (MQ=255)
                    cgcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGcc                                                                                                                           >  1:1217845/1‑150 (MQ=255)
                     gcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGccc                                                                                                                          <  1:696516/150‑1 (MQ=255)
                     gcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGccc                                                                                                                          <  1:696510/150‑1 (MQ=255)
                     gcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGccc                                                                                                                          <  1:391527/150‑1 (MQ=255)
                     gcgACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGccc                                                                                                                          <  1:858322/150‑1 (MQ=255)
                         ccACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCgaag                                                                                                                      >  2:1201145/1‑150 (MQ=255)
                           aCGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCgaagaa                                                                                                                    >  2:967610/1‑150 (MQ=255)
                           aCGGCTGCGGGTTCCTTCAGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCgaagaa                                                                                                                    <  2:930/150‑1 (MQ=255)
                              gCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAggg                                                                                                                 <  2:322932/150‑1 (MQ=255)
                                   gggTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATcc                                                                                                            >  2:65301/1‑150 (MQ=255)
                                              gggAACATCCAGATGGAAATACTGCGAATTTAGAAGTGATTCACTACATACGCAATGGTGCCTTGAGCTTTAGAGCGATTGTTTCTTTCGATCAATAAGTAGCCGGTTAACCAGTACAACAGCCCGAAGAAGGGGATCAACAGCAAGctc                                                                                                 >  2:738160/1‑150 (MQ=255)
                                              gggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGctc                                                                                                 >  2:738122/1‑150 (MQ=255)
                                              gggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGctc                                                                                                 <  1:658763/150‑1 (MQ=255)
                                               ggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGctct                                                                                                <  2:741034/150‑1 (MQ=255)
                                               ggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGctct                                                                                                <  2:740985/150‑1 (MQ=255)
                                                  aCATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCtttt                                                                                             <  1:678701/150‑1 (MQ=255)
                                                  aCATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCtttt                                                                                             <  1:315576/150‑1 (MQ=255)
                                                  aCATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCtttt                                                                                             <  1:315554/150‑1 (MQ=255)
                                                  aCATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCtttt                                                                                             <  1:1067038/150‑1 (MQ=255)
                                                  aCATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCtttt                                                                                             <  1:315514/150‑1 (MQ=255)
                                                    aTCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTta                                                                                           <  1:993656/150‑1 (MQ=255)
                                                     tCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTtac                                                                                          >  1:854667/1‑150 (MQ=255)
                                                     tCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTtac                                                                                          <  1:1282841/150‑1 (MQ=255)
                                                      ccAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTtacc                                                                                         >  2:998003/1‑150 (MQ=255)
                                                              aaaTACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTaccgtca                                                                                 <  2:355077/150‑1 (MQ=255)
                                                                aTACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTaccgtcacc                                                                               >  2:25930/1‑150 (MQ=255)
                                                                    ggCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcg                                                                           >  2:519075/1‑150 (MQ=255)
                                                                    ggCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcg                                                                           >  2:205872/1‑150 (MQ=255)
                                                                     gCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcgg                                                                          <  2:350627/150‑1 (MQ=255)
                                                                     gCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcgg                                                                          <  2:335313/150‑1 (MQ=255)
                                                                       gTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcggcg                                                                        <  1:676785/150‑1 (MQ=255)
                                                                       gTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcggcg                                                                        <  1:467475/150‑1 (MQ=255)
                                                                           tttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcgg                                                                          >  2:685905/1‑144 (MQ=255)
                                                                           tttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTcgg                                                                          <  1:685905/144‑1 (MQ=255)
                                                                                aGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGa                                                               <  2:822219/150‑1 (MQ=255)
                                                                                  tGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATg                                                             >  2:1188946/1‑150 (MQ=255)
                                                                                  tGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATg                                                             >  1:317630/1‑150 (MQ=255)
                                                                                    aTTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGtt                                                           >  1:1163187/1‑150 (MQ=255)
                                                                                    aTTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGtt                                                           >  1:1163215/1‑150 (MQ=255)
                                                                                    aTTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGtt                                                           >  2:173424/1‑150 (MQ=255)
                                                                                       cACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGa                                                        <  2:809803/150‑1 (MQ=255)
                                                                                       cACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGa                                                        <  2:1292630/150‑1 (MQ=255)
                                                                                       cACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGa                                                        <  2:1163689/150‑1 (MQ=255)
                                                                                        actactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGAt                                                       >  2:704914/1‑150 (MQ=255)
                                                                                        actactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGAt                                                       >  2:555386/1‑150 (MQ=255)
                                                                                         ctactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATg                                                      >  2:940550/1‑150 (MQ=255)
                                                                                         ctactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATg                                                      <  1:382755/150‑1 (MQ=255)
                                                                                          tactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGc                                                     >  1:1131173/1‑150 (MQ=255)
                                                                                                   aaTGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCAt                                            <  1:497344/150‑1 (MQ=255)
                                                                                                        tGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCGGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCAt                                       >  2:1361049/1‑150 (MQ=255)
                                                                                                             tGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAgttg                                  <  2:648162/150‑1 (MQ=255)
                                                                                                             tGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAgttg                                  <  1:1134289/150‑1 (MQ=255)
                                                                                                               aGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAgttgtt                                >  1:1155703/1‑150 (MQ=255)
                                                                                                                          gATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAg                     >  1:265800/1‑150 (MQ=255)
                                                                                                                           aTTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGc                    <  1:126736/150‑1 (MQ=255)
                                                                                                                           aTTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGc                    >  2:736018/1‑150 (MQ=255)
                                                                                                                              gTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGat                 <  2:669675/150‑1 (MQ=255)
                                                                                                                                ttCTGTCGATCAATAAGTTGCTGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGatat               <  2:233918/150‑1 (MQ=255)
                                                                                                                                ttCTGTCGATCAATAAGTTGCTGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGatat               <  2:994530/150‑1 (MQ=255)
                                                                                                                                ttCTGTCGATCAATAAGTTGCTGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACAATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGatat               <  2:233995/150‑1 (MQ=255)
                                                                                                                                ttCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGatat               >  2:35658/1‑150 (MQ=255)
                                                                                                                                ttCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGatat               >  2:1012149/1‑150 (MQ=255)
                                                                                                                                   tGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGTACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATataga            >  1:793449/1‑150 (MQ=255)
                                                                                                                                   tGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGTACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATataga            >  1:793455/1‑150 (MQ=255)
                                                                                                                                     tCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATatagata          <  1:1201145/150‑1 (MQ=255)
                                                                                                                                     tCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATatagata          >  1:772583/1‑150 (MQ=255)
                                                                                                                                     tCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTATACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACAATGTCATAGTTGTTCTGGTGGTTAGCGATatagata          >  2:1167802/1‑150 (MQ=255)
                                                                                                                                      cGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATatagatag         <  1:1127444/150‑1 (MQ=255)
                                                                                                                                       gATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGc        >  1:1197558/1‑150 (MQ=255)
                                                                                                                                       gATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGc        >  1:827976/1‑150 (MQ=255)
                                                                                                                                             tAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGcc  <  2:1284814/150‑1 (MQ=255)
                                                                                                                                             tAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGcc  <  1:1103277/150‑1 (MQ=255)
                                                                                                                                             tAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGcc  >  1:960569/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
AGTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACTGCCCGAAGAAGGGGATCCACAGCAAGCTCTTTTTACCTACCGTCACCGTCGGCGGTTGCACGATGTTCGATGCTGTCACCATGTCATAGTTGTTCTGGTGGTTAGCGATATAGATAGCATTGCC  >  NC_000913/3162999‑3163289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: