Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,898,844 T→C 8.2% M184V (ATG→GTG)  yieK ← putative 6‑phosphogluconolactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,898,8440TC 8.2% 311.9 / 14.8 98M184V (ATG→GTG) yieKputative 6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base T (45/45);  new base C (4/4);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 102 total aligned reads displayed.

GCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAA  >  NC_000913/3898696‑3898988
                                                                                                                                                     |                                                                                                                                                
gCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatg                                                                                                                                                 >  1:903536/1‑150 (MQ=255)
  ggCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgat                                                                                                                                               >  1:561941/1‑150 (MQ=255)
  ggCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgat                                                                                                                                               >  1:561900/1‑150 (MQ=255)
   gCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCatgatg                                                                                                                                              <  1:594519/150‑1 (MQ=255)
        aTCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCtttt                                                                                                                                         <  1:771156/150‑1 (MQ=255)
         tCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTc                                                                                                                                        <  1:346341/150‑1 (MQ=255)
         tCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTc                                                                                                                                        <  2:478224/150‑1 (MQ=255)
            gCGATTACCATCAGCGAAGGATGCAGCTGCAGCACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGc                                                                                                                                     <  2:719453/150‑1 (MQ=255)
            gCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGc                                                                                                                                     <  2:635775/150‑1 (MQ=255)
             cGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcc                                                                                                                                    <  2:733219/150‑1 (MQ=255)
             cGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGcc                                                                                                                                    <  2:1206784/150‑1 (MQ=255)
                  aCCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATc                                                                                                                               <  2:539921/150‑1 (MQ=255)
                   ccATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCg                                                                                                                              >  2:457450/1‑150 (MQ=255)
                    cATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGt                                                                                                                             >  2:442099/1‑150 (MQ=255)
                     aTCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTc                                                                                                                            <  1:605792/150‑1 (MQ=255)
                          cGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTa                                                                                                                       >  2:201416/1‑150 (MQ=255)
                          cGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTa                                                                                                                       >  1:287289/1‑150 (MQ=255)
                                 tGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCa                                                                                                                <  1:706130/150‑1 (MQ=255)
                                 tGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCa                                                                                                                <  1:706127/150‑1 (MQ=255)
                                      cTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCAc                                                                                                           >  2:1264134/1‑150 (MQ=255)
                                      cTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCAc                                                                                                           >  2:1264182/1‑150 (MQ=255)
                                          aaCACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAAc                                                                                                       <  1:261553/150‑1 (MQ=255)
                                           acacGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAAc                                                                                                       >  2:914232/1‑150 (MQ=255)
                                           acacGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACg                                                                                                      <  2:1262758/150‑1 (MQ=255)
                                             acGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaa                                                                                                    >  1:1113507/1‑150 (MQ=255)
                                              cGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGaaa                                                                                                   >  2:474991/1‑150 (MQ=255)
                                                gAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAg                                                                                                 >  2:656064/1‑150 (MQ=255)
                                                     tGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTcgcc                                                                                            <  1:808662/150‑1 (MQ=255)
                                                      ggAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTcgccg                                                                                           <  1:567092/150‑1 (MQ=255)
                                                      ggAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTcgccg                                                                                           <  1:567086/150‑1 (MQ=255)
                                                      ggAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTcgccg                                                                                           <  1:567082/150‑1 (MQ=255)
                                                         aCATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGccc                                                                                        <  2:200368/150‑1 (MQ=255)
                                                           aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCaa                                                                                      >  1:237914/1‑150 (MQ=255)
                                                           aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCaa                                                                                      >  1:237918/1‑150 (MQ=255)
                                                           aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCaa                                                                                      >  1:237926/1‑150 (MQ=255)
                                                           aTCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCaa                                                                                      >  1:237911/1‑150 (MQ=255)
                                                             cTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACt                                                                                    >  2:1179668/1‑150 (MQ=255)
                                                              ttCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTc                                                                                   <  1:201416/150‑1 (MQ=255)
                                                                 ggTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGcc                                                                                <  1:503808/150‑1 (MQ=255)
                                                                 ggTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGcc                                                                                >  1:240803/1‑150 (MQ=255)
                                                                  gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCa                                                                               >  2:752207/1‑150 (MQ=255)
                                                                  gTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCa                                                                               <  1:290709/150‑1 (MQ=255)
                                                                  gTAACCGGACCTTGAAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCa                                                                               >  2:802115/1‑150 (MQ=255)
                                                                     aCCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATgc                                                                            <  1:371193/150‑1 (MQ=255)
                                                                      ccGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATgcg                                                                           >  1:106400/1‑150 (MQ=255)
                                                                       cGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATgcgc                                                                          >  2:504055/1‑150 (MQ=255)
                                                                       cGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATgcgc                                                                          >  2:1346820/1‑150 (MQ=255)
                                                                       cGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATgcgc                                                                          >  2:1347439/1‑150 (MQ=255)
                                                                        ggACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAAc                                                                                     <  2:398108/138‑1 (MQ=255)
                                                                        ggACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAAc                                                                                     >  1:398108/1‑138 (MQ=255)
                                                                         gACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCca                                                                        <  2:602989/150‑1 (MQ=255)
                                                                         gACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCca                                                                        <  1:983989/150‑1 (MQ=255)
                                                                         gACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCca                                                                        <  1:984009/150‑1 (MQ=255)
                                                                          aCCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCcac                                                                       >  1:607537/1‑150 (MQ=255)
                                                                            cTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACaa                                                                     >  2:244233/1‑150 (MQ=255)
                                                                            cTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACaa                                                                     >  2:950335/1‑150 (MQ=255)
                                                                            cTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACaa                                                                     >  1:391621/1‑150 (MQ=255)
                                                                             ttGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAat                                                                    >  1:320776/1‑150 (MQ=255)
                                                                             ttGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAat                                                                    >  1:428245/1‑150 (MQ=255)
                                                                                 aGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCa                                                                >  2:1080419/1‑150 (MQ=255)
                                                                                      aTTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCAt                                                           <  1:1097292/150‑1 (MQ=255)
                                                                                      aTTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCAt                                                           <  1:392010/150‑1 (MQ=255)
                                                                                      aTTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCAt                                                           <  1:432923/150‑1 (MQ=255)
                                                                                       tttttCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCAtc                                                          >  2:821779/1‑150 (MQ=255)
                                                                                       tttttCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCAtc                                                          >  1:671709/1‑150 (MQ=255)
                                                                                            cAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTcc                                                     <  1:972471/150‑1 (MQ=255)
                                                                                                 ccTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGa                                                >  2:137296/1‑150 (MQ=255)
                                                                                                    gcgcTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCg                                             >  1:235032/1‑150 (MQ=255)
                                                                                                     cgcTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATctg                                            <  1:21481/150‑3 (MQ=255)
                                                                                                     cgcTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCgg                                            <  1:1060609/150‑1 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         >  2:188366/1‑150 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         >  2:188368/1‑150 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         >  1:598224/1‑150 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         <  2:237911/150‑1 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         <  2:237914/150‑1 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         <  2:237918/150‑1 (MQ=255)
                                                                                                        tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCACGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGaa                                         <  2:237926/150‑1 (MQ=255)
                                                                                                            cccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTcc                                     <  1:524252/150‑1 (MQ=255)
                                                                                                            cccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTcc                                     <  1:1077774/150‑1 (MQ=255)
                                                                                                              cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCAc                                   <  2:1327327/150‑1 (MQ=255)
                                                                                                              cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCAc                                   <  2:704319/150‑1 (MQ=255)
                                                                                                               gcgcCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACg                                  <  2:785032/150‑1 (MQ=255)
                                                                                                               gcgcCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACg                                  <  1:441772/150‑1 (MQ=255)
                                                                                                                     cTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAATTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGc                            >  2:1313479/1‑150 (MQ=255)
                                                                                                                      tGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCt                           >  1:1094495/1‑150 (MQ=255)
                                                                                                                        aCAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCa                         <  2:196311/150‑1 (MQ=255)
                                                                                                                               tCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAAt                  <  1:1196406/150‑1 (MQ=255)
                                                                                                                                cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATg                 <  2:577787/150‑1 (MQ=255)
                                                                                                                                  gCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATggg               >  2:49294/1‑150 (MQ=255)
                                                                                                                                   cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATCTCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGt              >  2:770992/1‑150 (MQ=255)
                                                                                                                                   cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGt              >  2:470501/1‑150 (MQ=255)
                                                                                                                                     aGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCg            >  1:361400/1‑150 (MQ=255)
                                                                                                                                      gTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGt           >  1:91988/1‑150 (MQ=255)
                                                                                                                                      gTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGt           >  1:91989/1‑150 (MQ=255)
                                                                                                                                        tttttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTa          >  1:1009125/1‑150 (MQ=255)
                                                                                                                                         ttttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAt         >  1:1175400/1‑150 (MQ=255)
                                                                                                                                          tttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCCTGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAtt        <  1:1311927/150‑1 (MQ=255)
                                                                                                                                          tttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAtt        <  1:1311953/150‑1 (MQ=255)
                                                                                                                                          tttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAtt        <  1:1042134/150‑1 (MQ=255)
                                                                                                                                                cgccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaa  <  1:474991/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCAGTGCCTGCGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAA  >  NC_000913/3898696‑3898988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: