| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,070,469 | A→C | 9.0% | intergenic (‑68/+46) | yihR ← / ← yihS | putative sulphoquinovose mutarotase/sulphoquinovose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,070,469 | 0 | A | C | 9.0% | 147.4 / 10.1 | 56 | intergenic (‑68/+46) | yihR/yihS | putative sulphoquinovose mutarotase/sulphoquinovose isomerase |
| Reads supporting (aligned to +/- strand): ref base A (36/10); new base C (3/2); total (40/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01 | |||||||||||
CCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATA > NC_000913/4070325‑4070617 | cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGTCACCTTAATTAACGACATCCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCTCCCCAAAGGGG‑CGAggg < 1:111265/150‑1 (MQ=255)cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCTCCCCAAAGGGG‑CGAggg < 1:111318/150‑1 (MQ=255)cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAggg > 1:615624/1‑150 (MQ=255) cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGtt > 1:361791/1‑150 (MQ=255) cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGtt > 1:361784/1‑150 (MQ=255) gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTa < 1:1158715/150‑1 (MQ=255) cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGga < 2:1083183/150‑1 (MQ=255) aGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGgaga < 2:1345794/150‑1 (MQ=255) tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACAGACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAgg < 2:254997/150‑1 (MQ=255) gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAggg < 2:723668/150‑1 (MQ=255) gAAACGGTCTGTCCACGGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAggg < 2:887260/150‑1 (MQ=255) aaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCCAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGGGGGGG‑AGAGGGGGAGGGAGAggggc > 2:740184/1‑149 (MQ=255) tCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAAcc > 1:1283344/1‑150 (MQ=255) tCTGACAACTGCAATGCGTATTGGTGATTTGCATACAAGGCGCCTGGTTTGAGGACTGGCAGCCTCCCGGGTGGCTGTAGTCAGACCCGCGGGCTGCAGGGGGCGGTCCGGTCGCGCGTGTGGGG‑TGTGGGGTTGGGGGGGGGGacctcg > 2:942341/1‑145 (MQ=255) tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGACAACCCGCa > 2:1069581/1‑150 (MQ=255) tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCa > 2:1342023/1‑150 (MQ=255) cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCAcc > 1:713300/1‑150 (MQ=255) cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCAcc > 1:841520/1‑150 (MQ=255) cAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtt > 1:535361/1‑150 (MQ=255) atTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATc > 1:204309/1‑150 (MQ=255) ggTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTCGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGt > 1:113868/1‑150 (MQ=255) gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt > 1:595492/1‑150 (MQ=255) gATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAt > 2:1395718/1‑150 (MQ=255) gCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 2:182207/1‑150 (MQ=255) aaGGCACCTTAATTAACCACATACATCCACCCGTGAGGCAGATTTCAAACACAAAAACTGCACCGGACGGTACCCTCGCACATTTGCGG‑AGAGGGTTAGGGAGAGGGGAAAACCAGCACCCTATTGTAAGATAAGTTATTTCGCATTAat > 2:624112/1‑150 (MQ=255) cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 2:872171/1‑150 (MQ=255) ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca > 2:913424/1‑150 (MQ=255) ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca > 2:1338543/1‑150 (MQ=255) aattacCGCCTTACCGCCTCCCGTGTGGCTGTTGTCAAACCCAAAAACGGCCCCGGACGGACCCCCCGCCCCTTTGGGG‑TGCGGGTTAGGGAGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTATTATCCAGCAGa < 2:702829/150‑1 (MQ=255) aCATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCTTTACTATCCAGCAGACCCGCTGc > 1:1068960/1‑150 (MQ=255) aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa > 1:1043445/1‑150 (MQ=255) aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa < 2:916231/150‑1 (MQ=255) tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGa > 2:928993/1‑150 (MQ=255) tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGa > 2:928973/1‑150 (MQ=255) cGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGCCGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTTGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc < 2:1288470/150‑1 (MQ=255) cGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc < 2:1288469/150‑1 (MQ=255) tgatgaAAAACCCAAAACCCCCACCGGCCGGCCCCCCCGCCCCGTGGGGG‑TGAGGTTTCGGGTGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCggg < 1:1375867/150‑1 (MQ=255) tGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCggg > 2:896808/1‑150 (MQ=255) ccacaaaaCCGCCCCGGTCGGGCCCCCCGCCCCGTTGGGGTTTTTGTTTTGGTTGCGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACgg < 2:13078/146‑1 (MQ=255) aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa > 2:83109/1‑150 (MQ=255) aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAGCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa > 2:1021452/1‑150 (MQ=255) cACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGAt > 1:642300/1‑150 (MQ=255) cctaacccTCTCCCCAAAGGGG‑CGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATACAGCAGACCCGCAGCAACCGCAGGAGCCATGCCGGGCGCTAACGGGAAACGCGGGATCACCAgg > 1:926842/6‑150 (MQ=255) gggccccTCGCCCTTTGGGGG‑TGTGGGTTCGGGAGTGGGTAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGc < 1:866445/147‑1 (MQ=255) ggTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAGCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGc > 2:1078606/1‑150 (MQ=255) gTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCa > 1:232743/1‑150 (MQ=255) tCCCCTCGCCCGTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCaa < 2:341379/150‑1 (MQ=255) tCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCaa > 1:222847/1‑150 (MQ=255) tCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCaa > 1:529048/1‑150 (MQ=255) tCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCaa > 1:1230563/1‑150 (MQ=255) acccTCTCCCCAAAGGGG‑CGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt > 2:563397/2‑150 (MQ=255) cTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgca > 2:311583/1‑150 (MQ=255) cccaaagggg‑CGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAgg > 1:698882/7‑150 (MQ=255) tttGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGa > 1:1164030/1‑150 (MQ=255) gg‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAat > 2:710623/1‑150 (MQ=255) gg‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAat > 2:1153733/1‑150 (MQ=255) g‑agaGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAata > 1:548332/1‑150 (MQ=255) | CCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATA > NC_000913/4070325‑4070617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |