Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,283,905 G→A 5.9% A333V (GCG→GTG)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,9050GA 5.9% 346.5 / 8.5 102A333V (GCG→GTG) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base G (54/42);  new base A (2/4);  total (56/46)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 105 total aligned reads displayed.

GCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAG  >  NC_000913/4283758‑4284051
                                                                                                                                                   |                                                                                                                                                  
gcgGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCccgcc                                                                                                                                                  <  1:600823/150‑1 (MQ=255)
gcgGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCccgcc                                                                                                                                                  <  1:885602/150‑1 (MQ=255)
  ggATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCcgccgc                                                                                                                                                >  1:848439/1‑150 (MQ=255)
      gcgcCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAACTAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCt                                                                                                                                            >  2:1132957/1‑150 (MQ=255)
      gcgcCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCt                                                                                                                                            <  1:1011803/150‑1 (MQ=255)
       cgcccgccAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCtt                                                                                                                                           <  1:597145/150‑1 (MQ=255)
       cgcccgccAGCGTAAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCCCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATACGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCtt                                                                                                                                           >  2:728000/1‑150 (MQ=255)
         cccgccAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTta                                                                                                                                         <  2:526616/150‑1 (MQ=255)
              cAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTc                                                                                                                                    >  2:97874/1‑150 (MQ=255)
              cAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTc                                                                                                                                    >  1:677502/1‑150 (MQ=255)
                gCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCg                                                                                                                                  >  1:90723/1‑150 (MQ=255)
                gCGTCAGACACGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCg                                                                                                                                  >  1:90721/1‑150 (MQ=255)
                     aGACCCGCAACCACCGCGAGGTTAGTGGCGTAAGCAACCGGTGTAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTc                                                                                                                             <  2:85799/150‑1 (MQ=255)
                      gACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCg                                                                                                                            <  2:255397/150‑1 (MQ=255)
                      gACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCg                                                                                                                            <  1:321042/150‑1 (MQ=255)
                       aCCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGc                                                                                                                           >  2:730388/1‑150 (MQ=255)
                         ccGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCac                                                                                                                         >  1:786798/1‑150 (MQ=255)
                         ccGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCac                                                                                                                         >  1:257510/1‑150 (MQ=255)
                             aaCCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCaccaac                                                                                                                     <  2:334497/150‑1 (MQ=255)
                                   cgcgAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCAt                                                                                                               >  1:729746/1‑150 (MQ=255)
                                   cgcgAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCAt                                                                                                               >  1:143191/1‑150 (MQ=255)
                                    gcgAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATg                                                                                                              >  1:933712/1‑150 (MQ=255)
                                    gcgAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATg                                                                                                              >  1:933694/1‑150 (MQ=255)
                                      gAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGAt                                                                                                            <  1:218565/150‑1 (MQ=255)
                                         gATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATcgc                                                                                                         >  2:785433/1‑150 (MQ=255)
                                         gATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATcgc                                                                                                         >  2:785386/1‑150 (MQ=255)
                                            aGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGcc                                                                                                      >  1:268926/1‑150 (MQ=255)
                                             gTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCg                                                                                                     >  1:1313627/1‑150 (MQ=255)
                                              tGGCGAAAGCAACCGCTGAAATAAAACCGAGGACCAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGa                                                                                                    >  2:54019/1‑150 (MQ=255)
                                                gCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAg                                                                                                  >  2:1058926/1‑150 (MQ=255)
                                                gCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCAACTACATTCGCCAGGTGAACGTCCGCCATGTTGTTAACACAAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAg                                                                                                  >  2:595993/1‑150 (MQ=255)
                                                         aCCGCTGAAATAAAACCGACGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGataata                                                                                         <  1:105807/150‑1 (MQ=255)
                                                           cGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATaaa                                                                                       <  1:684790/150‑1 (MQ=255)
                                                           cGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATaaa                                                                                       <  1:738796/150‑1 (MQ=255)
                                                           cGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATaaa                                                                                       <  1:738838/150‑1 (MQ=255)
                                                              tGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGt                                                                                    <  2:241583/150‑1 (MQ=255)
                                                              tGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGt                                                                                    <  2:1306709/150‑1 (MQ=255)
                                                                   tAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGaa                                                                               <  1:20484/150‑1 (MQ=255)
                                                                      aaCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAatat                                                                            >  1:1219373/1‑150 (MQ=255)
                                                                      aaCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAatat                                                                            <  2:691514/150‑1 (MQ=255)
                                                                       aCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAAtata                                                                           >  1:828723/1‑150 (MQ=255)
                                                                        ccGAGGATCAGGTTGCCGCCCACTGCATTCGCCAGGTGTACGGCCGCCATGTTGTTACCACCTATCAGATGGCCCGCCGCGTCTTTATTTTCCGGAGTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAg                                                                          <  1:1220315/150‑1 (MQ=255)
                                                                        ccGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAg                                                                          >  1:451006/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  2:869320/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:273720/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  2:869295/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  1:1063779/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  1:1398423/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:1192298/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  2:481184/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:851756/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  1:1063773/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        >  2:869328/1‑150 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:376374/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:1145424/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:376371/150‑1 (MQ=255)
                                                                          gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                        <  1:376343/150‑1 (MQ=255)
                                                                             gAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTa                                                                     >  1:467771/1‑150 (MQ=255)
                                                                             gAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTa                                                                     >  1:986293/1‑150 (MQ=255)
                                                                                  ggTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCa                                                                >  2:890532/1‑150 (MQ=255)
                                                                                   gTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCAt                                                               >  1:304507/1‑150 (MQ=255)
                                                                                    ttGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATa                                                              >  2:513876/1‑150 (MQ=255)
                                                                                         gccCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCTCCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAAccc                                                         <  1:703491/150‑1 (MQ=255)
                                                                                         gccCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAAccc                                                         >  1:329182/1‑150 (MQ=255)
                                                                                         gccCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAAccc                                                         >  1:329176/1‑150 (MQ=255)
                                                                                          cccACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCg                                                        >  2:1030057/1‑150 (MQ=255)
                                                                                          cccACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATAAAGAAGAAGCCCATAAACCCg                                                        >  2:1184119/1‑150 (MQ=255)
                                                                                            cACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGt                                                      <  2:994708/150‑1 (MQ=255)
                                                                                             aCTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTg                                                     <  2:302929/150‑1 (MQ=255)
                                                                                              cTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTgg                                                    >  1:1269509/1‑150 (MQ=255)
                                                                                              cTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTgg                                                    <  2:766114/150‑1 (MQ=255)
                                                                                               tGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGc                                                   <  1:206882/150‑1 (MQ=255)
                                                                                               tGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGc                                                   >  2:742087/1‑150 (MQ=255)
                                                                                                  aTTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTa                                                <  2:526952/150‑1 (MQ=255)
                                                                                                     cGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGaa                                             <  2:90723/150‑1 (MQ=255)
                                                                                                     cGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGaa                                             <  2:90721/150‑1 (MQ=255)
                                                                                                      gCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAac                                            >  2:366654/1‑150 (MQ=255)
                                                                                                           gTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGct                                       >  1:496677/1‑150 (MQ=255)
                                                                                                                   ccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTg                               <  1:810359/150‑1 (MQ=255)
                                                                                                                   ccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTg                               >  2:1384681/1‑150 (MQ=255)
                                                                                                                   ccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTg                               <  2:7672/150‑1 (MQ=255)
                                                                                                                   ccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTg                               <  2:779848/150‑1 (MQ=255)
                                                                                                                      ccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCtt                            >  2:791860/1‑150 (MQ=255)
                                                                                                                          gttgttACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                        <  2:90495/150‑1 (MQ=255)
                                                                                                                          gttgttACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAAGATAGAAGTAGCCCATCCACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                        <  1:263242/150‑1 (MQ=255)
                                                                                                                                accaccAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATc                  >  1:1187343/1‑150 (MQ=255)
                                                                                                                                  caccaATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGc                <  1:167682/150‑1 (MQ=255)
                                                                                                                                   accaATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt               >  2:1279429/1‑150 (MQ=255)
                                                                                                                                   accaATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt               >  1:128623/1‑150 (MQ=255)
                                                                                                                                   accaATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt               >  2:1279417/1‑150 (MQ=255)
                                                                                                                                     cactCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGa             <  2:1332170/147‑1 (MQ=255)
                                                                                                                                     caATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGa             <  2:722576/150‑1 (MQ=255)
                                                                                                                                          aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTa        >  2:569870/1‑150 (MQ=255)
                                                                                                                                           gATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTaa       <  1:730388/150‑1 (MQ=255)
                                                                                                                                           gATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGAGAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTaa       >  2:522369/1‑150 (MQ=255)
                                                                                                                                            aTGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGACATCGCTGACTGTaaa      <  1:1138367/150‑1 (MQ=255)
                                                                                                                                               gCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaa   >  2:1011490/1‑150 (MQ=255)
                                                                                                                                               gCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaa   <  1:1175685/150‑1 (MQ=255)
                                                                                                                                               gCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaa   <  1:379167/150‑1 (MQ=255)
                                                                                                                                                cccACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaag  >  2:426877/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAG  >  NC_000913/4283758‑4284051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑