Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,092,779 Δ1 bp 6.8% coding (902/951 nt) gshB → glutathione synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,092,7790T. 6.8% 282.4 / 12.7 88coding (902/951 nt)gshBglutathione synthetase
Reads supporting (aligned to +/- strand):  ref base T (43/39);  new base . (4/2);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGA  >  NC_000913/3092631‑3092926
                                                                                                                                                     |                                                                                                                                                   
tGGAAAATCGCCCGTCCGATCTGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGAt                                                                                                                                                     <  2:1026643/149‑1 (MQ=255)
tGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTTGTCTGGATATCATCGGAGACCGTCTGACTGAAATTAACGTAACCATCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGAt                                                                                                                                                     >  2:1536314/1‑149 (MQ=255)
tGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCTGAGTTTCCGGTGTCGAt                                                                                                                                                     <  2:272398/149‑1 (MQ=255)
tGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGAt                                                                                                                                                     >  1:784059/1‑149 (MQ=255)
  gAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCa                                                                                                                                                   >  1:306027/1‑149 (MQ=255)
   aaaaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAc                                                                                                                                                  <  1:895156/149‑1 (MQ=255)
    aaaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAcc                                                                                                                                                 <  2:1541099/149‑1 (MQ=255)
     aaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGCTCACCg                                                                                                                                                >  1:86680/1‑149 (MQ=255)
             gTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTa                                                                                                                                        >  2:533494/1‑149 (MQ=255)
              tCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                                                       >  1:513688/1‑149 (MQ=255)
               cAGATCGGGCCGACGCTGAAAGAAACAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGATCGACACCGGaaactctgc                                                                                                                                      <  1:1069173/149‑8 (MQ=255)
                 gATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATgg                                                                                                                                    <  2:153884/149‑1 (MQ=255)
                    cGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATg                                                                                                                                 <  1:1112298/149‑1 (MQ=255)
                            cGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGaa                                                                                                                         <  1:912670/149‑1 (MQ=255)
                            cGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGaa                                                                                                                         <  2:515450/149‑1 (MQ=255)
                             gCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAg                                                                                                                        <  2:1050616/149‑1 (MQ=255)
                                gaaagaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCAc                                                                                                                     <  1:1239778/149‑1 (MQ=255)
                                 aaagaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCACCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGGTAATGGATGCCATCGAAGCACg                                                                                                                    >  2:81583/1‑149 (MQ=255)
                                 aaagaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACg                                                                                                                    >  2:133115/1‑149 (MQ=255)
                                 aaagaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACg                                                                                                                    >  2:574994/1‑149 (MQ=255)
                                   agaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGtt                                                                                                                  <  2:687787/149‑1 (MQ=255)
                                        aaGGGCTGATTGTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcag                                                                                                             <  2:1178319/149‑1 (MQ=255)
                                         aGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagc                                                                                                            >  1:1568194/1‑149 (MQ=255)
                                           ggCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcag                                                                                                          <  2:1390844/149‑1 (MQ=255)
                                           ggCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcag                                                                                                          <  1:1354194/149‑1 (MQ=255)
                                            gCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                                         >  2:263051/1‑149 (MQ=255)
                                                aTTTTTGTTGGTCTGGATATCATCGGCGACCGTCTCACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACAGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCACTa                                                                                                     >  2:448017/1‑149 (MQ=255)
                                                          gTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTa                                                                                           >  1:1407699/1‑149 (MQ=255)
                                                          gTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTa                                                                                           >  1:1151087/1‑149 (MQ=255)
                                                          gTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTa                                                                                           <  1:1966/149‑1 (MQ=255)
                                                           tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                                          >  2:566224/1‑149 (MQ=255)
                                                             tGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCg                                                                                        >  1:711465/1‑149 (MQ=255)
                                                             tGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCg                                                                                        >  1:957907/1‑149 (MQ=255)
                                                              ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                                                       >  2:474193/1‑149 (MQ=255)
                                                              ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                                                       <  2:1066763/149‑1 (MQ=255)
                                                               gATATCATCGTCGACCGTCTGACTGAAATTAACGTCACCAGCCCCACCTCTATTCCTGAGAGTGAAGCAGACTTTCCGCTGTCTCTCACCGGAAAGTTAATCGACGCCATCGAGGCACGTTTACAGCAGCAGTAACCCACCTAATCgag                                                                                      >  2:844444/1‑149 (MQ=255)
                                                                     atcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAt                                                                                >  2:465466/1‑149 (MQ=255)
                                                                      tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc                                                                               >  2:309214/1‑149 (MQ=255)
                                                                         gCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCg                                                                            <  2:784059/149‑1 (MQ=255)
                                                                            aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCCCCTTAGCGAGAAGGATCTCGTTg                                                                         <  2:86680/149‑1 (MQ=255)
                                                                                 ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGAct                                                                    >  2:1315941/1‑149 (MQ=255)
                                                                                     ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                                >  1:432149/1‑149 (MQ=255)
                                                                                     ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                                <  2:306027/149‑1 (MQ=255)
                                                                                      tgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAg                                                               >  2:476770/1‑149 (MQ=255)
                                                                                       gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGt                                                              <  1:263051/149‑1 (MQ=255)
                                                                                        aaaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTg                                                             >  1:1074709/1‑149 (MQ=255)
                                                                                          aTTAACGTCACCAGCCCAACATGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAC                                                           >  1:459990‑M1/1‑149 (MQ=255)
                                                                                           ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACA                                                          >  2:1295018‑M1/1‑149 (MQ=255)
                                                                                                gTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTTAGACTCTGAGTGACAGCGCC                                                     >  1:176441‑M1/1‑149 (MQ=255)
                                                                                                 tCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCC                                                    >  1:506758‑M1/1‑149 (MQ=255)
                                                                                                   aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAGTGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCctt                                                  <  1:1386212/149‑1 (MQ=255)
                                                                                                   aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCctt                                                  >  2:868904/1‑149 (MQ=255)
                                                                                                    ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTGTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCcttc                                                 >  2:910651/1‑149 (MQ=255)
                                                                                                        cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                             <  1:1536314/149‑1 (MQ=255)
                                                                                                        cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                             <  2:1568194/149‑1 (MQ=255)
                                                                                                        cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                             <  1:186170/149‑1 (MQ=255)
                                                                                                        cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACAGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                             >  1:1133781/1‑149 (MQ=255)
                                                                                                          cAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                           <  1:723317/149‑1 (MQ=255)
                                                                                                           aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCAc                                          <  1:133115/149‑1 (MQ=255)
                                                                                                           aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCAc                                          <  1:518057/149‑1 (MQ=255)
                                                                                                              cTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCACTTCTTTCAACGCa                                       >  2:987896/1‑149 (MQ=255)
                                                                                                                    tCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATTCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                                 >  2:199487‑M2/1‑147 (MQ=255)
                                                                                                                    tCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCagtatgcgtggaaagaagg                                 <  2:1585942‑M1/149‑20 (MQ=255)
                                                                                                                       tGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgggcg                              <  1:414419‑M2/149‑6 (MQ=255)
                                                                                                                            ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGtt                         <  1:1236124/149‑1 (MQ=255)
                                                                                                                             tGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTg                        <  2:711465/149‑1 (MQ=255)
                                                                                                                             tGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTg                        <  2:957907/149‑1 (MQ=255)
                                                                                                                              gAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCagtatgcgtggaaagaagggcgctgtcac                       <  2:1231728‑M1/149‑30 (MQ=255)
                                                                                                                               aaGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCt                      >  1:411469/1‑149 (MQ=255)
                                                                                                                               aaGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCt                      <  1:1315941/149‑1 (MQ=255)
                                                                                                                                aGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtt                     >  1:604986/1‑149 (MQ=255)
                                                                                                                                aGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtt                     >  2:695716/1‑149 (MQ=255)
                                                                                                                                 gCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCagtatgcgtggaaagaagggcgctgtcactca                    <  2:584979‑M1/149‑33 (MQ=255)
                                                                                                                                 gCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt                    <  2:1456350/149‑1 (MQ=255)
                                                                                                                                 gCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt                    <  2:1365012/149‑1 (MQ=255)
                                                                                                                                 attaacattCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt                    >  1:495872/8‑149 (MQ=255)
                                                                                                                                 attaacattCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt                    >  2:714744/8‑149 (MQ=255)
                                                                                                                                  ttaacattccggtgaTCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                                 >  1:645836‑M2/7‑133 (MQ=255)
                                                                                                                                  ttaacattccggtgaTCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                                 <  2:645836‑M2/129‑3 (MQ=255)
                                                                                                                                  cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtttt                   >  2:34875/1‑149 (MQ=255)
                                                                                                                                   agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttttt                  >  1:1412032/1‑149 (MQ=255)
                                                                                                                                    gagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtttttt                 >  1:1257821/1‑149 (MQ=255)
                                                                                                                                    gagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtttttt                 >  2:923379/1‑149 (MQ=255)
                                                                                                                                     agTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCagtatgcgtggaaagaagggcgctgtcactcagagt                <  1:452020‑M1/149‑37 (MQ=255)
                                                                                                                                       tttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa              >  2:1176707/1‑149 (MQ=255)
                                                                                                                                       attCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCCCCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa              >  1:287363/2‑149 (MQ=255)
                                                                                                                                            ggTGTCGATCACCGGAATGATAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGAAGTTTCTTTTTTGAACCAgg         >  1:200298/1‑149 (MQ=255)
                                                                                                                                             gtgtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGa        <  2:287041/149‑1 (MQ=255)
                                                                                                                                               gtCGATCACCGGAATGTTAATGGAGGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGaaa      <  1:1578290/149‑1 (MQ=255)
                                                                                                                                                   gATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg   >  2:473519/1‑149 (MQ=255)
                                                                                                                                                   gATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg   <  1:910615/149‑1 (MQ=255)
                                                                                                                                                    aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGAAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTACTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGa  >  1:107987/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGA  >  NC_000913/3092631‑3092926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: