Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,865:1 +G 6.5% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8651.G 6.5% 314.3 / 16.6 77intergenic (+23/‑90)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base . (38/34);  new base G (3/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGC  >  NC_000913/1868717‑1869011
                                                                                                                                                     |                                                                                                                                                  
aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt                                                                                                                                                     <  2:53511/149‑1 (MQ=255)
aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTATGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCATAACAACCCTGGCCCGGTACACATGt                                                                                                                                                     >  2:316671/1‑149 (MQ=255)
    cGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                                                                                >  2:1434949/1‑149 (MQ=255)
      tCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCgg                                                                                                                                              <  1:1154421/149‑1 (MQ=255)
        aaCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                                                                            <  1:11804/149‑1 (MQ=255)
         aCCGACGAGCAGACGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                                                                           <  1:609495/149‑1 (MQ=255)
          ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCt                                                                                                                                          >  2:741270/1‑149 (MQ=255)
          ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCt                                                                                                                                          >  1:756236/1‑149 (MQ=255)
            gacgaGCAGAAGAAACACGACGACTTTGTCGACCGTAAGAAGGAAAAACGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAAAGGTAATTGCGCGTACGTAAATCGTCTTAACAACACTGGCCCGGTACACATGTTACCGGGACTAc                                                                                                                                        >  2:1173907/1‑149 (MQ=255)
              cgaGCATAAGACACACGTCGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACaa                                                                                                                                      <  1:259634/149‑1 (MQ=255)
               gaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGAAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAAc                                                                                                                                     >  2:930168/1‑149 (MQ=255)
                 gCAGAAGAAACACGACGACTTTGTCGGCCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACAGGGCCTACACCGa                                                                                                                                   >  2:1005110/1‑149 (MQ=255)
                 gCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGa                                                                                                                                   >  2:1465068/1‑149 (MQ=255)
                 gCAGAAGAAACACGACGACTTAGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGAGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGa                                                                                                                                   >  1:858666/1‑149 (MQ=255)
                  cAGAAGAAACACGACGACATAGTCGACCGTATGAAGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGAGCGAATGGTATTTGCGCGTACGTAAATCGTAATAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAAAACGAc                                                                                                                                  >  2:1427044/1‑149 (MQ=255)
                    gaagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                                                                                <  1:1072446/149‑1 (MQ=255)
                       gaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGAGCGAATGGTATTTGCGCGTACGTAAATCGTCTTACCCACCCTGGCCCGGTACACATGTTAGCGGTCCTACAACGACAGCgc                                                                                                                             >  1:64719/1‑148 (MQ=255)
                          acacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAcc                                                                                                                          >  1:1268753/1‑149 (MQ=255)
                            acgacgACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                                                                        <  2:510712/149‑1 (MQ=255)
                            acgacgACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                                                                        >  2:1023212/1‑149 (MQ=255)
                               acgacTTTGTCGACCGTATGATGGAAAAATGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                                                                     <  2:851210/149‑1 (MQ=255)
                                 gacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGcc                                                                                                                   <  1:951633/149‑1 (MQ=255)
                                      tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                                                                         <  1:1018232/122‑3 (MQ=255)
                                      tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                                                                         >  2:1018232/1‑120 (MQ=255)
                                         agACCGTATTATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACTTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACTACAGCGAACCGTGGGCCTGAGAAGc                                                                                                           <  1:822894/148‑1 (MQ=255)
                                            ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                                                                        <  2:707288/149‑1 (MQ=255)
                                             cGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGAGCGTACGTAAATCGTCTTAAAAACCATGTCCCGGTACAAATGTTACCGGGCCTACAACTACAGCGAACCGTGGGCCTGAGCAGCGGCa                                                                                                       >  1:268564/1‑149 (MQ=255)
                                              gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa                                                                                                      <  1:1434949/149‑1 (MQ=255)
                                               tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGAATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACACTGGGCCTGAGAAGCGGCAac                                                                                                     <  2:259391/149‑1 (MQ=255)
                                                   atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                                                                                 >  2:1508635/1‑149 (MQ=255)
                                                   atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                                                                                 <  1:575487/149‑1 (MQ=255)
                                                     ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                                                                               >  1:582/1‑149 (MQ=255)
                                                      gAAAAAGGCTACACCCGTAAACAGGAGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                                                                              >  2:1582235/1‑149 (MQ=255)
                                                        aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa                                                                                            <  2:374515/149‑1 (MQ=255)
                                                           aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCa                                                                                         <  1:216411/149‑1 (MQ=255)
                                                               tACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                                                                                     <  1:1119884/149‑1 (MQ=255)
                                                                  acCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                                                                                  >  1:1294022/1‑149 (MQ=255)
                                                                     cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                                                                               <  1:898202/149‑1 (MQ=255)
                                                                            aGGTGCGTTTACTGTGCGAATGGTATTTGCTCGTAGGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCTAACCGTTGGCCTGAGATGCGGCAACACAGGCGTAGCATTCAGTTGGCAAATgta                                                                        <  1:1439148/149‑1 (MQ=255)
                                                                            aGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgta                                                                        >  2:847628/1‑149 (MQ=255)
                                                                             ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                                                                       <  2:756236/149‑1 (MQ=255)
                                                                                   tttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGTTACACATGTTACAGGGCCTAAAACGACAGCGAACCGTGTGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATCTAGTACgtg                                                                 >  1:86322/1‑147 (MQ=255)
                                                                                   tttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                 >  2:336837/1‑149 (MQ=255)
                                                                                   tttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                 >  1:1578026/1‑149 (MQ=255)
                                                                                   tttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                 >  2:1025583/1‑149 (MQ=255)
                                                                                        tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa                                                            >  2:585261/1‑149 (MQ=255)
                                                                                        tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCGGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa                                                            >  2:803604/1‑149 (MQ=255)
                                                                                          tgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata                                                          >  1:408519/1‑149 (MQ=255)
                                                                                           gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCatat                                                         >  2:1090675/1‑149 (MQ=255)
                                                                                              aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGCCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc                                                      >  2:396540/1‑149 (MQ=255)
                                                                                                tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt                                                    <  2:582/149‑1 (MQ=255)
                                                                                                tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt                                                    <  1:312088/149‑1 (MQ=255)
                                                                                                 ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg                                                   <  1:1465068/149‑1 (MQ=255)
                                                                                                  ttATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTgg                                                  <  1:1582235/148‑1 (MQ=255)
                                                                                                      ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                              <  1:741270/149‑1 (MQ=255)
                                                                                                       tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAGCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACTTGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                             <  1:1427044/149‑1 (MQ=255)
                                                                                                       tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                             <  1:336837/149‑1 (MQ=255)
                                                                                                                gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCg                                    >  2:542054/1‑149 (MQ=255)
                                                                                                                 taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCgg                                   >  1:1209246/1‑148 (MQ=255)
                                                                                                                  aaaTCGTCTTAACAACCCTGGCCCGGTACACATGATACCGGGCCTTCAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTc                                  <  2:298069/149‑1 (MQ=255)
                                                                                                                   aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCt                                 >  2:725307/1‑149 (MQ=255)
                                                                                                                   aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCGGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAACTGGTTTATTGACCGGCGTCt                                 >  1:866653/1‑149 (MQ=255)
                                                                                                                          ttAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGc                          <  1:316671/149‑1 (MQ=255)
                                                                                                                          ttAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGc                          >  1:459654/1‑149 (MQ=255)
                                                                                                                           tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGCACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCa                         >  1:539434/1‑149 (MQ=255)
                                                                                                                             acaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATGTGACCTGGTTTATTGACCGGCGTCTGAACGGCaaa                       >  1:17268/1‑149 (MQ=255)
                                                                                                                                acCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGTGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAAc                    <  2:914495/149‑1 (MQ=255)
                                                                                                                                   cTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa                 <  1:1508635/149‑1 (MQ=255)
                                                                                                                                    tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCTTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGGCTGAACGGCAAAAACaaaa                <  2:858666/149‑1 (MQ=255)
                                                                                                                                      gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGc              >  1:1518412/1‑149 (MQ=255)
                                                                                                                                       cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCGTGAGAAGCGGCAACACAGGCGTAGCAAACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTATATTGACCGGCGTCTGAAAGGCAAAAACAAAAGCa             >  2:789374/1‑149 (MQ=255)
                                                                                                                                       cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCa             >  2:450672/1‑149 (MQ=255)
                                                                                                                                        ccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt            >  1:1213728/1‑149 (MQ=255)
                                                                                                                                          ggTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg          <  1:1530753/149‑1 (MQ=255)
                                                                                                                                           gTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGt         <  1:1005110/149‑1 (MQ=255)
                                                                                                                                               acaTTTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAAt     <  1:725307/149‑1 (MQ=255)
                                                                                                                                               acaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCACACACAAAAGCATGGTCaaa     >  1:117401/1‑148 (MQ=255)
                                                                                                                                                 aTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCg   >  2:461902/1‑149 (MQ=255)
                                                                                                                                                  tGTTACCGGGCCTACAACGACAGCGATCCGTGGTCCTGAGTAGCGGAAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTTTTGACCTGCGTCTGAACGGCAAAAACAAAAGCTTGGTGAATCGc  <  1:1173907/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGC  >  NC_000913/1868717‑1869011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: