Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,366,010:1 +T 5.2% coding (83/1140 nt) arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,366,0101.T 5.2% 468.2 / 18.0 116coding (83/1140 nt)arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base . (51/59);  new base T (2/4);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 118 total aligned reads displayed.

AAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGAT  >  NC_000913/2365862‑2366157
                                                                                                                                                     |                                                                                                                                                   
aaaTATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGCCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGAGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTg                                                                                                                                                      >  2:820632/1‑149 (MQ=255)
   tataCACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGAt                                                                                                                                                  <  2:1561274/149‑1 (MQ=255)
   tataCACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGAt                                                                                                                                                  <  1:396908/149‑1 (MQ=255)
       cacaACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTAGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATcaca                                                                                                                                              <  2:819729/149‑1 (MQ=255)
        acaacaCTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACaa                                                                                                                                             >  1:46777/1‑149 (MQ=255)
        acaacaCTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACaa                                                                                                                                             >  2:4464/1‑149 (MQ=255)
          aacaCTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAAcc                                                                                                                                           >  1:506521/1‑149 (MQ=255)
          aacaCTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAAcc                                                                                                                                           >  2:577216/1‑149 (MQ=255)
             acTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGTGATCCAACCgga                                                                                                                                        <  1:366253/149‑2 (MQ=255)
               tctTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTcc                                                                                                                                      >  2:1448183/1‑149 (MQ=255)
               tctTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTcc                                                                                                                                      >  1:1243398/1‑149 (MQ=255)
                 tttATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCga                                                                                                                                    >  2:1324330/1‑149 (MQ=255)
                  ttATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGTGATCCAACCggattcga                                                                                                                                   <  1:1354197/149‑7 (MQ=255)
                  ttATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCgaa                                                                                                                                   <  2:511400/149‑1 (MQ=255)
                      aGCAAATATAAGTGGACAGGTATTCAATGGCTGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAAt                                                                                                                               <  1:1131071/149‑1 (MQ=255)
                      aGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCTGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAAt                                                                                                                               >  1:1575016/1‑149 (MQ=255)
                      aGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAAt                                                                                                                               <  1:599790/149‑1 (MQ=255)
                      aGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAAt                                                                                                                               <  1:1452460/149‑1 (MQ=255)
                       gCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATc                                                                                                                              >  2:1102490/1‑149 (MQ=255)
                       gCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATc                                                                                                                              >  2:1154029/1‑149 (MQ=255)
                        cAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGTCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCa                                                                                                                             >  1:1111255/1‑149 (MQ=255)
                        cAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCa                                                                                                                             >  2:198854/1‑149 (MQ=255)
                        cAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCa                                                                                                                             >  1:648793/1‑149 (MQ=255)
                        cAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCa                                                                                                                             >  1:1071946/1‑149 (MQ=255)
                         aaaTATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAg                                                                                                                            <  1:716044/149‑1 (MQ=255)
                         aaaTATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAg                                                                                                                            <  1:791253/149‑1 (MQ=255)
                           atatAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGgc                                                                                                                          >  1:271680/1‑149 (MQ=255)
                               aaGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCtt                                                                                                                      <  2:1513749/149‑1 (MQ=255)
                               aaGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCtt                                                                                                                      <  2:1391169/149‑1 (MQ=255)
                               aaGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCtt                                                                                                                      <  2:1377261/149‑1 (MQ=255)
                                 gTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGa                                                                                                                    >  1:136847/1‑149 (MQ=255)
                                  tGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGCATCAGGCGCTTGAg                                                                                                                   >  2:1423525/1‑149 (MQ=255)
                                         gTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCtt                                                                                                            >  1:1519974/1‑149 (MQ=255)
                                          tATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttt                                                                                                           <  1:4464/149‑1 (MQ=255)
                                          tATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttt                                                                                                           >  1:46459/1‑149 (MQ=255)
                                          tATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttt                                                                                                           <  1:1310678/149‑1 (MQ=255)
                                           aTTCAATGTCTGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCATCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCtttt                                                                                                          <  1:1114806/149‑1 (MQ=255)
                                           aTTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCtttt                                                                                                          <  2:462453/149‑1 (MQ=255)
                                           aTTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCtttt                                                                                                          <  1:1097212/149‑1 (MQ=255)
                                           aTTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCtttt                                                                                                          >  2:197202/1‑149 (MQ=255)
                                            ttCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTTCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttttt                                                                                                         <  1:657134/149‑1 (MQ=255)
                                            ttCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttttt                                                                                                         >  2:1227220/1‑149 (MQ=255)
                                            ttCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCttttt                                                                                                         <  2:767729/149‑1 (MQ=255)
                                               aaTGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGTACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACAGGTCCGAAGAATCAGTCGCTTGAGCAAGCTTTTTGcc                                                                                                      >  2:514666/1‑149 (MQ=255)
                                               aaTGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGcc                                                                                                      >  1:1044940/1‑149 (MQ=255)
                                               aaTGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGcc                                                                                                      >  1:808163/1‑149 (MQ=255)
                                                aTGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCa                                                                                                     <  1:1488929/149‑1 (MQ=255)
                                                 tGGCGGAAGGAAAAGCACTGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGGGGAGGAACTCGCTGCAGTTACAGAGGTGCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAg                                                                                                    <  1:674406/149‑1 (MQ=255)
                                                 tGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAg                                                                                                    <  2:350043/149‑1 (MQ=255)
                                                   gCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGtt                                                                                                  >  1:1114299/1‑149 (MQ=255)
                                                     ggaaggaaAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGa                                                                                                >  1:899476/1‑149 (MQ=255)
                                                       aaggaaAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACg                                                                                              <  1:627917/149‑1 (MQ=255)
                                                           aaaaGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGaa                                                                                          <  1:808204/149‑1 (MQ=255)
                                                           aaaaGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGaa                                                                                          >  1:331277/1‑149 (MQ=255)
                                                            aaaGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGaaa                                                                                         <  2:1590479/149‑1 (MQ=255)
                                                             aaGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAAt                                                                                        >  2:1500760/1‑149 (MQ=255)
                                                                 aaTGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGc                                                                                    >  1:800643/1‑149 (MQ=255)
                                                                 aaTGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGATGGATCACAACAGGTCAGAAGAATCAGGCGCTTGATCAAGCATTTTGCCAGTTGACTGGAAATCAGc                                                                                    >  1:94275/1‑149 (MQ=255)
                                                                  aTGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCa                                                                                   <  2:865267/149‑1 (MQ=255)
                                                                   tGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCAt                                                                                  <  1:879278/149‑1 (MQ=255)
                                                                    gTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATg                                                                                 >  1:1417354/1‑149 (MQ=255)
                                                                     tCAGAATTTTTGCCTTTTTCGCGACCGGCAATGGGCGTGGAGGAACTCGCCGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGc                                                                                >  1:1326469/1‑149 (MQ=255)
                                                                     tCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGc                                                                                >  1:1177685/1‑149 (MQ=255)
                                                                       aGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCa                                                                              >  2:1093045/1‑149 (MQ=255)
                                                                        gAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCAt                                                                             >  1:441411/1‑149 (MQ=255)
                                                                             tttGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCgg                                                                        <  1:1102490/149‑1 (MQ=255)
                                                                              ttGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGt                                                                       <  1:1324330/149‑1 (MQ=255)
                                                                                 ccTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAg                                                                    <  1:1518082/149‑1 (MQ=255)
                                                                                  cTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGt                                                                   >  1:265296/1‑149 (MQ=255)
                                                                                     tttCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCa                                                                <  1:1436759/149‑1 (MQ=255)
                                                                                          cgACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCAc                                                           <  2:46777/149‑1 (MQ=255)
                                                                                             ccAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCAccgc                                                        >  2:516499/1‑149 (MQ=255)
                                                                                                   aTGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAAt                                                  <  1:381430/149‑1 (MQ=255)
                                                                                                    tGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATg                                                 <  2:800643/149‑1 (MQ=255)
                                                                                                      ggCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                               <  2:1100259/149‑1 (MQ=255)
                                                                                                       gCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCat                                              >  2:1172227/1‑149 (MQ=255)
                                                                                                         gTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCatat                                            <  2:693400/149‑1 (MQ=255)
                                                                                                         gTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCatat                                            <  2:412570/149‑1 (MQ=255)
                                                                                                                    cGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATgg                                 <  1:1172227/149‑1 (MQ=255)
                                                                                                                     gCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGc                                <  1:1357329/149‑1 (MQ=255)
                                                                                                                        gCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGtt                             <  1:1054441/149‑1 (MQ=255)
                                                                                                                          aGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGa                           <  2:1330099/149‑1 (MQ=255)
                                                                                                                               aaGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAAtt                      <  2:808163/149‑1 (MQ=255)
                                                                                                                                agagGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCGGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTg                     >  1:1304606/1‑149 (MQ=255)
                                                                                                                                agagGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTg                     <  2:136847/149‑1 (MQ=255)
                                                                                                                                  agGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGTATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCGACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGc                   <  1:1420075/149‑1 (MQ=255)
                                                                                                                                    gTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCaa                 <  1:577216/149‑1 (MQ=255)
                                                                                                                                    gTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTAGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCaa                 <  1:89824/149‑1 (MQ=255)
                                                                                                                                       ctcGAATCCGGATGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAggg              <  2:1417354/149‑1 (MQ=255)
                                                                                                                                        tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGCCGGGTAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCCCGCTAATGGCGTTGAAAATTGGCACGGGc             >  2:787517/1‑149 (MQ=255)
                                                                                                                                        tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGc             >  1:1119105/1‑149 (MQ=255)
                                                                                                                                          gAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATg                                                 <  2:1199443/111‑1 (MQ=255)
                                                                                                                                           aaTCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCTCGGTCAGTTCAGCCACCACCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGAt          <  2:1519974/149‑1 (MQ=255)
                                                                                                                                             tCCGTTTGGATCACAACCGGTCCGAAGAATGAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGATAATGGCGTTGAAAATTGGCAAGGTCGATGa        <  2:1152372/149‑1 (MQ=255)
                                                                                                                                             accGGTTGTGATCCAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCatat                                            >  2:825839/2‑113 (MQ=255)
                                                                                                                                             accGGTTGTGATCCAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCatat                                            <  1:825839/112‑1 (MQ=255)
                                                                                                                                             accGGTTGTGATCCAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGa        >  2:831305/2‑149 (MQ=255)
                                                                                                                                                ggTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGAt          >  1:973127/1‑144 (MQ=255)
                                                                                                                                                ggTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGAt          <  2:973127/144‑1 (MQ=255)
                                                                                                                                                   tGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGAt  <  2:1243398/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGAT  >  NC_000913/2365862‑2366157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: