Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 933,155 T→C 7.2% intergenic (+66/‑69) lrp → / → ftsK leucine‑responsive global transcriptional regulator/DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,1550TC 7.2% 249.7 / 7.3 85intergenic (+66/‑69)lrp/ftsKleucine‑responsive global transcriptional regulator/DNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base T (44/33);  new base C (2/4);  total (47/38)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

CTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTTGC  >  NC_000913/933010‑933302
                                                                                                                                                 |                                                                                                                                                   
cTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAATTCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACtcgt                                                                                                                                                  >  2:69500/1‑146 (MQ=255)
cTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTggg                                                                                                                                                  >  2:20737/1‑149 (MQ=255)
 tGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTgggg                                                                                                                                                 >  1:1597621/1‑149 (MQ=255)
 tGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTgggg                                                                                                                                                 >  1:1237389/1‑149 (MQ=255)
  ggCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGt                                                                                                                                                <  2:373581/149‑1 (MQ=255)
    cGTCAATGACACACGTACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCTGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTaa                                                                                                                                              <  1:82145/149‑1 (MQ=255)
      tCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGTCGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAAcc                                                                                                                                            >  1:290614/1‑149 (MQ=255)
           gACACACGGACATACGTTGTTATGGAAGAAGTCTAGGAGATTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTa                                                                                                                                       <  2:1451896/149‑1 (MQ=255)
                acGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgtt                                                                                                                                  <  1:466074/149‑1 (MQ=255)
                 cGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACtgttg                                                                                                                                 >  2:840604/1‑149 (MQ=255)
                   gACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgttgct                                                                                                                               <  1:745809/149‑3 (MQ=255)
                    aCATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTcc                                                                                                                              >  2:208174/1‑149 (MQ=255)
                    aCATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTcc                                                                                                                              <  2:399462/149‑1 (MQ=255)
                       tACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgttgctgtat                                                                                                                           <  2:918322/149‑7 (MQ=38)
                        aCGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGttt                                                                                                                          <  1:483472/149‑1 (MQ=255)
                        aCGTTGTTATGGAAGAAGTAAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGAGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACATGGTACTGTTGTCCGttt                                                                                                                          >  2:824055/1‑149 (MQ=255)
                         cGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGtttt                                                                                                                         >  1:11671/1‑149 (MQ=255)
                         cGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGtttt                                                                                                                         <  1:20737/149‑1 (MQ=255)
                          tttgttATGGAAGAAGTCAATCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTa                                                                                                                        <  1:168916/148‑1 (MQ=255)
                          tttgtgATGGAAGCCGGCAAGCCGAGGAATCGTCTGGTTATTAAGACGCGCGAACAAGGAACAGGGGCAAAATCGGCGTATTTTGATTACCCTCCTGTGAATCCATACAGCAACAGTACTGGGGGAAACTGGTACTGTTGTCCGTGTTa                                                                                                                        <  1:168917/148‑1 (MQ=255)
                          gttgttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTa                                                                                                                        <  2:650274/149‑1 (MQ=255)
                          gttgttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTa                                                                                                                        >  1:541319/1‑149 (MQ=255)
                           ttgttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAg                                                                                                                       >  2:1268387/1‑149 (MQ=255)
                             gttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCa                                                                                                                     >  1:820596/1‑149 (MQ=255)
                                aTGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCg                                                                                                                  >  1:627613/1‑149 (MQ=255)
                                aTGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCg                                                                                                                  <  2:1358505/149‑1 (MQ=255)
                                 tGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGCCGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGATTAAACATCgg                                                                                                                 >  1:1047242/1‑149 (MQ=255)
                                 tGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCgg                                                                                                                 >  1:1134596/1‑149 (MQ=255)
                                  ggAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCggg                                                                                                                <  1:1136012/149‑1 (MQ=255)
                                   gaagaaGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGc                                                                                                               >  1:969806/1‑149 (MQ=255)
                                    aagaagTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCa                                                                                                              >  1:385363/1‑149 (MQ=255)
                                    aagaagTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCa                                                                                                              >  2:809698/1‑149 (MQ=255)
                                            aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGc                                                                                                      >  1:1486335/1‑149 (MQ=255)
                                            aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGc                                                                                                      >  1:16605/1‑149 (MQ=255)
                                            aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGc                                                                                                      >  2:1502141/1‑149 (MQ=255)
                                             aGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGAACAGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATAGGGCAGGTAAAGcc                                                                                                     >  2:1033917/1‑149 (MQ=255)
                                              gCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCt                                                                                                    <  1:302323/149‑1 (MQ=255)
                                                agagTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACACCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGt                                                                                                  >  1:275296/1‑149 (MQ=255)
                                                    tAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAAcc                                                                                              >  2:1168501/1‑149 (MQ=255)
                                                    tAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCATGTAAcc                                                                                              >  1:836212/1‑149 (MQ=255)
                                                      aTCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTg                                                                                            <  2:1525970/149‑1 (MQ=255)
                                                       tCGTCTGGTTAATAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTgg                                                                                           >  1:230096/1‑149 (MQ=255)
                                                                tattaAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCttt                                                                                  <  2:842968/149‑1 (MQ=255)
                                                                 attaAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTc                                                                                 <  1:517533/149‑1 (MQ=255)
                                                                     aGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGa                                                                             >  1:1456123/1‑149 (MQ=255)
                                                                       aCGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGc                                                                           <  1:208174/149‑1 (MQ=255)
                                                                       aCGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGc                                                                           >  2:1211572/1‑149 (MQ=255)
                                                                         gcgcTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCa                                                                         <  1:594497/149‑1 (MQ=255)
                                                                          cgcTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAg                                                                        <  2:1375676/149‑1 (MQ=255)
                                                                               acacGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATa                                                                   <  2:541319/149‑1 (MQ=255)
                                                                               acacGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCAAGGAATa                                                                   >  1:1071574/1‑149 (MQ=255)
                                                                                  cGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACAt                                                                <  2:290614/149‑1 (MQ=255)
                                                                                   ggAACAGGTGCAAAATCGGCGTTTTTTGATTACACTGCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACAtt                                                               <  2:268013/149‑1 (MQ=255)
                                                                                   ggAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCCGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACAtt                                                               <  2:1300095/149‑1 (MQ=255)
                                                                                     aaCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGa                                                             >  1:1378684/1‑149 (MQ=255)
                                                                                     aaCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGa                                                             >  1:599186/1‑149 (MQ=255)
                                                                                      aCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGaa                                                            <  1:1065664/149‑1 (MQ=255)
                                                                                      aCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGaa                                                            <  2:284380/149‑1 (MQ=255)
                                                                                      aCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGaa                                                            <  2:627613/149‑1 (MQ=255)
                                                                                           tGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTTTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAAACTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACaa                                                       >  1:444411/1‑149 (MQ=255)
                                                                                           tGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATAGGGCAGGAAAAGCCTGTAACCTTGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACaa                                                       >  2:1600699/1‑149 (MQ=255)
                                                                                                 aTCGGCGTATATTGATTACACTCCTGTTACTCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGt                                                 <  2:215248/149‑1 (MQ=255)
                                                                                                      cGTTTTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGTCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACAt                                            <  1:816638/149‑1 (MQ=255)
                                                                                                       gTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACGGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACAtt                                           <  1:572253/149‑1 (MQ=255)
                                                                                                         aTTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGa                                         <  1:133463/149‑1 (MQ=255)
                                                                                                          ttttGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGAc                                        >  1:1094926/1‑149 (MQ=255)
                                                                                                              gATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAATAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAg                                    <  2:16605/149‑1 (MQ=255)
                                                                                                                  acacTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTaa                                <  1:1168501/149‑1 (MQ=255)
                                                                                                                      tCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAg                            >  1:447514/1‑149 (MQ=255)
                                                                                                                        cTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCg                          <  2:1237389/149‑1 (MQ=255)
                                                                                                                         tGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCgg                         >  2:750365/1‑149 (MQ=255)
                                                                                                                         tGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCgg                         >  1:832775/1‑149 (MQ=255)
                                                                                                                          gTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgc                        >  2:1095199/1‑149 (MQ=255)
                                                                                                                             aaTCCATACAGCAACAGTACTGGGGTAACATGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCAGTAACCTGGAGAGCCTTTCTTTAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAATGTTTAGTAGCGgccgc                     >  2:244564/1‑149 (MQ=255)
                                                                                                                              aTCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccgcc                    >  2:268765/1‑149 (MQ=255)
                                                                                                                              aTCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccgcc                    >  2:548456/1‑149 (MQ=255)
                                                                                                                                ccATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccgccgc                  <  1:809698/149‑1 (MQ=255)
                                                                                                                                ccATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccgccgc                  >  1:357119/1‑149 (MQ=255)
                                                                                                                                    cggacAACAGTACCAGGTTACCCCAGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTc              >  1:1277143/5‑149 (MQ=255)
                                                                                                                                      gacAACAGTACCAGGTTACCCCAGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaagaa                                                   >  1:119195/3‑110 (MQ=38)
                                                                                                                                      gacAACAGTACCAGGTTACCCCAGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaagaa                                                   <  2:119195/108‑1 (MQ=38)
                                                                                                                                      aGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTg            >  1:454324/1‑149 (MQ=255)
                                                                                                                                        cAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGa          <  2:1134596/149‑1 (MQ=255)
                                                                                                                                        cAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTATAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAATAAAAAGAAGACACATTGACAAAGTTAAGTAGCGGCCGACGCCTTCTGGa          >  2:230152/1‑149 (MQ=255)
                                                                                                                                              tACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGtt    >  1:1032119/1‑149 (MQ=255)
                                                                                                                                                cTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTTGc  >  1:1532596/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTTGC  >  NC_000913/933010‑933302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: