Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 964,150 T→A 6.7% intergenic (+38/‑170) ihfB → / → ycaI integration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913964,1500TA 6.7% 355.6 / 12.4 120intergenic (+38/‑170)ihfB/ycaIintegration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (54/58);  new base A (2/6);  total (56/64)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

Only 100 of 120 total aligned reads displayed.

ACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCAC  >  NC_000913/964005‑964294
                                                                                                                                                 |                                                                                                                                                
aCCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCgg                                                                                                                                               >  2:455446/1‑149 (MQ=255)
 ccGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCggg                                                                                                                                              >  2:543532/1‑149 (MQ=255)
  cGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGt                                                                                                                                             >  1:558718/1‑149 (MQ=255)
      cGTAATCCGACGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCCCCCGACAGGTGCtt                                                                                                                                         <  1:1550225/149‑1 (MQ=255)
        ttaTCCGAAGACTGGGGATAAATTAGAACTGGAAGGAAAATTCGTTCCTCACTTTAAACCTTGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCCGACAGGTGCtttt                                                                                                                                       <  2:856172/147‑1 (MQ=255)
         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCttttt                                                                                                                                      >  2:966041/1‑149 (MQ=255)
         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCttttt                                                                                                                                      <  2:680235/149‑1 (MQ=255)
         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCttttt                                                                                                                                      <  2:1297383/149‑1 (MQ=255)
         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCttttt                                                                                                                                      >  1:1485290/1‑149 (MQ=255)
          aTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCtttttt                                                                                                                                     <  1:1011730/149‑1 (MQ=255)
          aTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCtttttt                                                                                                                                     <  1:1551192/149‑1 (MQ=255)
          aTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTAATCAAACTTGAACGAGAGAAAAGCACATGTCGGGTGCtttttt                                                                                                                                     >  1:388322/1‑149 (MQ=255)
           tCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTc                                                                                                                                    >  1:302844/1‑149 (MQ=255)
            ccGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCa                                                                                                                                   <  1:840528/149‑1 (MQ=255)
            ccGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTACCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCa                                                                                                                                   <  2:399129/149‑1 (MQ=255)
             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCAt                                                                                                                                  >  2:941973/1‑149 (MQ=255)
             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCAt                                                                                                                                  <  2:1474700/149‑1 (MQ=255)
             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCAt                                                                                                                                  >  2:1101104/1‑149 (MQ=255)
             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCCGACAGGTGCTTTtctctc                                                                                                                                  <  1:1036306/149‑3 (MQ=255)
               aaGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCAttt                                                                                                                                >  2:1461864/1‑149 (MQ=255)
               aaGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCAttt                                                                                                                                <  2:404300/149‑1 (MQ=255)
                aGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCCGACAGGTGCTTTtctctcgtt                                                                                                                               <  2:1579465/149‑6 (MQ=255)
                 gACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAATTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTtct                                                                                                                              >  2:1145156/1‑149 (MQ=255)
                  aCTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTtct                                                                                                                              <  2:665073/148‑1 (MQ=255)
                  aCTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTtct                                                                                                                              >  1:665073/1‑148 (MQ=255)
                   cTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAACCCTGGTAAATAACTGAGCGATCGCGCCAATATTTACGTTTAAGTATTATACTCAAACTTAAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTAAGTtctca                                                                                                                            >  1:293340/1‑148 (MQ=255)
                      gCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAAt                                                                                                                         >  2:269581/1‑149 (MQ=255)
                      gCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAAt                                                                                                                         >  2:1317492/1‑149 (MQ=255)
                       cGATAAAGTAGAACTGGATGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATc                                                                                                                        >  1:258847/1‑149 (MQ=255)
                         aTAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctg                                                                                                                      <  1:455446/149‑1 (MQ=255)
                          tAAAGTATAACTGGAAGGAAACTACGTTCCTCACTTTAAACCTTGTAAAGAACTTCGCGATCGCGCCAAAATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctgg                                                                                                                     <  1:252638/149‑1 (MQ=255)
                             aGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTAATCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTAATTTCTCTAATctggaac                                                                                                                  >  1:1196088/1‑149 (MQ=255)
                              gTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctggaact                                                                                                                 <  2:685542/149‑1 (MQ=255)
                              gTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctggaact                                                                                                                 >  1:47483/1‑149 (MQ=255)
                                 gAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctggaactgga                                                                                                              >  2:242926/1‑149 (MQ=255)
                                  aaCTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATctggaactggaa                                                                                                             >  2:205480/1‑149 (MQ=255)
                                   aCTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAg                                                                                                            <  2:1403948/149‑1 (MQ=255)
                                      ggaaggaaAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTg                                                                                                         <  2:1485290/149‑1 (MQ=255)
                                       gaaggaaAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGc                                                                                                        >  1:247259/1‑149 (MQ=255)
                                       gaaggaaAATACGGTCCTCACTTTAAACCTGGTAAAGACCTGCGCGAGCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGc                                                                                                        <  2:153668/149‑1 (MQ=255)
                                         aggaaAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCt                                                                                                      >  1:1345382/1‑149 (MQ=255)
                                         aggaaAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCt                                                                                                      <  2:396879/149‑1 (MQ=255)
                                           gaaAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGc                                                                                                   >  1:1024432/1‑149 (MQ=255)
                                              aaTACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCag                                                                                                 >  1:167385/1‑149 (MQ=255)
                                                 aCGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTTATCTGGAACTGGTAGCTGCCTCGCAGAGt                                                                                              >  2:1091824/1‑149 (MQ=255)
                                                 aCGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGt                                                                                              >  2:566838/1‑149 (MQ=255)
                                                   gTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAtttt                                                                                            >  2:1054000/1‑149 (MQ=255)
                                                    ttCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGtttt                                                                                           <  1:892031/149‑1 (MQ=255)
                                                    ttCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGtttt                                                                                           <  1:11115/149‑1 (MQ=255)
                                                    ttCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGtttt                                                                                           <  1:966041/149‑1 (MQ=255)
                                                       cTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGaa                                                                                        <  2:1087792/149‑1 (MQ=255)
                                                       cTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGaa                                                                                        >  1:1485587/1‑149 (MQ=255)
                                                        tCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAAc                                                                                       >  2:1457348/1‑149 (MQ=255)
                                                          aCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAg                                                                                     >  2:839830/1‑149 (MQ=255)
                                                              tAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGtttt                                                                                 <  2:1533842/149‑1 (MQ=255)
                                                                 aCCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCAc                                                                              >  1:1046190/1‑149 (MQ=255)
                                                                   cTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCAccc                                                                            >  2:1247020/1‑149 (MQ=255)
                                                                     ggTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCtt                                                                          <  1:670965/149‑1 (MQ=255)
                                                                     ggTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCtt                                                                          <  1:533337/149‑1 (MQ=255)
                                                                      gTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCttt                                                                         <  2:62024/149‑1 (MQ=255)
                                                                       tAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTc                                                                        <  1:205480/149‑1 (MQ=255)
                                                                         aaGAACTGCGCGATCGCGCCAATATTTAAGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAAATGGAAGCTGCCTCGCAGAGTTTTTAACAGTTTTCACCCTTTCGt                                                                      >  2:543682/1‑149 (MQ=255)
                                                                          aGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGGGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGtt                                                                     <  1:1092748/149‑1 (MQ=255)
                                                                             aCTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTaaa                                                                  <  2:623819/149‑1 (MQ=255)
                                                                                 cgcgATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAAttct                                                              >  2:670824/1‑149 (MQ=255)
                                                                                  gcgATCGCGCCAATATTTACTGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAAttctt                                                             <  1:1593356/149‑1 (MQ=255)
                                                                                    gATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATtcttct                                                           >  1:1514352/1‑149 (MQ=255)
                                                                                     aTCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTg                                                          <  2:558718/149‑1 (MQ=255)
                                                                                       cgcgCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGTAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGaa                                                        <  1:1003330/149‑1 (MQ=255)
                                                                                            cAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTGATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAAGAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGc                                                   <  1:1528074/149‑1 (MQ=255)
                                                                                            cAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGc                                                   <  1:537846/149‑1 (MQ=255)
                                                                                              atatTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCt                                                 <  2:247259/149‑1 (MQ=255)
                                                                                                     cGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGaa                                          <  1:1509901/149‑1 (MQ=255)
                                                                                                     cGGTTAAGGTTTTTACTCAAACTTGACCGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGaa                                          <  2:323131/149‑1 (MQ=255)
                                                                                                        ttAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGc                                       >  1:1375951/1‑149 (MQ=255)
                                                                                                           aGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGTGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCATCGGGGAACGCaaa                                    <  2:613695/149‑1 (MQ=255)
                                                                                                            gTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCaaaa                                   <  1:543532/149‑1 (MQ=255)
                                                                                                             ttttttACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAAt                                  <  1:1416905/149‑1 (MQ=255)
                                                                                                                tttACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc                               <  2:1173874/149‑1 (MQ=255)
                                                                                                                tttACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc                               <  2:1052211/149‑1 (MQ=255)
                                                                                                                tttACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc                               <  1:1101104/149‑1 (MQ=255)
                                                                                                                tttACTCAAAATTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc                               <  2:1196088/149‑1 (MQ=255)
                                                                                                                  tACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCcca                             <  2:749210/149‑1 (MQ=255)
                                                                                                                   aCTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCccac                            <  2:1345382/149‑1 (MQ=255)
                                                                                                                   aCTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCccac                            <  1:1317492/149‑1 (MQ=255)
                                                                                                                     tCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCccacca                          >  1:454209/1‑149 (MQ=255)
                                                                                                                      cAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAg                         >  1:547614/1‑149 (MQ=255)
                                                                                                                       aaaCTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAAATGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGa                        >  1:866591/1‑149 (MQ=255)
                                                                                                                            tGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAAcc                   <  1:942845/149‑1 (MQ=255)
                                                                                                                              aaCGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGc                 >  1:977034/1‑149 (MQ=255)
                                                                                                                                 atgaaaaaaGCACCCGACAGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTca              >  2:764900/6‑149 (MQ=255)
                                                                                                                                  agagaAAAGCACATGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTcaa             <  2:302844/149‑1 (MQ=255)
                                                                                                                                   gagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTcaac            >  1:452130/1‑149 (MQ=255)
                                                                                                                                    agaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTcaaca           >  1:118478/1‑149 (MQ=255)
                                                                                                                                    agaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTcaaca           >  2:203265/1‑149 (MQ=255)
                                                                                                                                    agaAAAGCACCTGTCGGGTGCTTTTTTCATTTATCTAATCAGGAACAGGAAGCTGCCTCGCAGAGTTTTGAACAGTATTCACCCTTTCCTTAAATTCTTCTTAATATGCCTCGGGGAACGCAAAAATCCCACCAGACAAACGCacaaca           >  2:578667/1‑149 (MQ=255)
                                                                                                                                     gaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGACCGCAAAATTCCCACCAGACAACCGCTcaacaa          >  2:962952/1‑149 (MQ=255)
                                                                                                                                      aaaaGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACaaa         <  2:455095/149‑1 (MQ=255)
                                                                                                                                           cACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGc    >  1:1054273/1‑149 (MQ=255)
                                                                                                                                             ccTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGcac  <  2:680983/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCAC  >  NC_000913/964005‑964294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: