Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 176,977 A→G 5.1% intergenic (+23/+24) erpA → / ← yadS iron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913176,9770AG 5.1% 308.5 / 4.2 98intergenic (+23/+24)erpA/yadSiron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (48/45);  new base G (2/3);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 102 total aligned reads displayed.

TGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATG  >  NC_000913/176830‑177125
                                                                                                                                                   |                                                                                                                                                    
tGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGTAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATaaa                                                                                                                                                     <  1:824783/149‑1 (MQ=255)
tGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATaaa                                                                                                                                                     <  2:244896/149‑1 (MQ=255)
 gAGCCTGGAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAc                                                                                                                                                    <  2:884731/149‑1 (MQ=255)
      tGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCTGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTTCCGGATAAACTATcc                                                                                                                                               >  1:248448/1‑149 (MQ=255)
        cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCTTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                             <  2:305669/149‑1 (MQ=255)
        cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                             <  2:1461260/149‑1 (MQ=255)
        cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                             <  2:250953/149‑1 (MQ=255)
        cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                             <  2:166180/149‑1 (MQ=255)
           tatCTGGTCGGCGGTTCCGTTGATTATACCGAATGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCAGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGcac                                                                                                                                          <  1:194658/149‑1 (MQ=255)
           tatCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGcac                                                                                                                                          >  1:388825/1‑149 (MQ=255)
              gtGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAACAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACAtc                                                                                                                                       <  1:105471/148‑1 (MQ=255)
              cTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCTGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGCACAtg                                                                                                                                       <  1:782828/149‑2 (MQ=255)
               tGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACAtct                                                                                                                                      >  1:1191175/1‑149 (MQ=255)
                  tCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACAtcttct                                                                                                                                   >  1:101168/1‑149 (MQ=255)
                    ggcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGCACAtgaacaac                                                                                                                                 <  1:945644/149‑8 (MQ=255)
                    ggcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctct                                                                                                                                 <  2:1191175/149‑1 (MQ=255)
                     gcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctctc                                                                                                                                >  1:1259550/1‑149 (MQ=255)
                     gcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctctc                                                                                                                                <  1:488173/149‑1 (MQ=255)
                     gcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGAACATCTtctctc                                                                                                                                <  2:278786/149‑1 (MQ=255)
                     gcggTGCCGTTGTTTATACCCAAGGTGTGTAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAACGCACGTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctctc                                                                                                                                <  2:1201350/149‑1 (MQ=255)
                       ggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTTTCTAATCTGTTGTTCATGTGCCGTATAAACTATCCGGCACATCTTCTCTCtt                                                                                                                              <  1:1588244/149‑1 (MQ=255)
                        gTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTc                                                                                                                             >  1:1124094/1‑149 (MQ=255)
                         ttCCGTTGATTATACCGATGGTCTGGAAGGTTCTCGTTTCTTAGTGACCATCCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCATTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCa                                                                                                                            <  1:1008177/149‑1 (MQ=255)
                          tCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCaa                                                                                                                           <  2:1296963/149‑1 (MQ=255)
                           cagTTGATTATACAGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGTTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCAGGATAAACTATCCCGCACATCTTCTCTCTTCAAc                                                                                                                          >  2:1141321/3‑149 (MQ=255)
                              ttGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                       >  2:1060017/1‑149 (MQ=255)
                                   tataCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtc                                                                                                                  <  1:1255428/149‑1 (MQ=255)
                                   tataCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtc                                                                                                                  <  1:584192/149‑1 (MQ=255)
                                    ataCCGAAGGTCTGGATGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATTTGCCGGATAATCTATCCGGCACATCTTCTCTCTTCAACGCCCATtct                                                                                                                 >  2:1294252/1‑149 (MQ=255)
                                     taCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtctc                                                                                                                <  2:939505/149‑1 (MQ=255)
                                     taCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtctc                                                                                                                >  1:609598/1‑149 (MQ=255)
                                        cGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGCACAtgaa                                                                                                                                     <  1:186284/125‑4 (MQ=255)
                                        cGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGCACAtgaa                                                                                                                                     >  2:186284/1‑122 (MQ=255)
                                        cGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATc                                                                                                             <  1:1531982/149‑1 (MQ=255)
                                        cGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATc                                                                                                             >  1:442802/1‑149 (MQ=255)
                                        cGAAGGTCTGGAAGGTGCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGTTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATc                                                                                                             <  2:1165912/149‑1 (MQ=255)
                                             gTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGGACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAgcg                                                                                                        >  2:1374309/1‑149 (MQ=255)
                                                tGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCCTCCAGCGCaa                                                                                                     >  2:1179445/1‑149 (MQ=255)
                                                tGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCaa                                                                                                     >  2:669464/1‑149 (MQ=255)
                                                tGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCaa                                                                                                     >  1:313630/1‑149 (MQ=255)
                                                 ggAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCaaa                                                                                                    >  2:750232/1‑149 (MQ=255)
                                                 ggAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCAAATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCaaa                                                                                                    <  1:1310803/149‑1 (MQ=255)
                                                  gAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATTTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAAc                                                                                                   >  2:1226060/1‑149 (MQ=255)
                                                  gAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAAc                                                                                                   >  1:1398417/1‑149 (MQ=255)
                                                   aaGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACg                                                                                                  >  1:805098/1‑149 (MQ=255)
                                                    aGGTTCTCGTTTCATCGTGACCAACCCTAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGt                                                                                                 <  2:694950/149‑1 (MQ=255)
                                                    aGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGt                                                                                                 <  2:586995/149‑1 (MQ=255)
                                                     ggTTATCGTTTCATCGTGAACAACCCGAACGCGAAACGCACCTGCGGTTGCGTTTCTTACTTTAGTATCTAATCTGTTGTTAATGTGCCTGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCAATTCTCATCCAGCGCAAACGTc                                                                                                <  2:14818/149‑1 (MQ=255)
                                                        tctcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGt                                                                                             >  2:298543/1‑149 (MQ=255)
                                                         ctcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTa                                                                                            <  2:286555/149‑1 (MQ=255)
                                                          tcatttCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCAATCTCATCCAGCGCAAACGGCGGTAg                                                                                           <  1:772694/146‑1 (MQ=255)
                                                          tcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAg                                                                                           >  1:656682/1‑149 (MQ=255)
                                                          tcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAg                                                                                           >  1:856065/1‑149 (MQ=255)
                                                             tttCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCtt                                                                                        >  1:754742/1‑149 (MQ=255)
                                                               tCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTaa                                                                                      <  2:434157/149‑1 (MQ=255)
                                                                cATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAg                                                                                     <  1:782079/149‑1 (MQ=255)
                                                                  tCGTGATCAACCCGAACGCGAAAAGCACCTGCGGTTGCTGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGAt                                                                                   <  1:285631/149‑1 (MQ=255)
                                                                  tCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGAt                                                                                   <  2:101168/149‑1 (MQ=255)
                                                                    gTGAACATCCCGAACGCGACAAGGACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGc                                                                                 <  2:125037/149‑1 (MQ=255)
                                                                        ccAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAAc                                                                             <  1:750232/149‑1 (MQ=255)
                                                                           aCCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGaa                                                                          <  2:1141237/149‑1 (MQ=255)
                                                                             ccGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATa                                                                        >  2:1379447/1‑149 (MQ=255)
                                                                             ccGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGAACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAGCGTCGGTAGCTTAAGATGCCAACGaaaa                                                                        >  1:1186880/1‑147 (MQ=255)
                                                                              cGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAg                                                                       <  2:313630/149‑1 (MQ=255)
                                                                              cGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAg                                                                       <  1:684762/149‑1 (MQ=255)
                                                                                             ccTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCa                                                        >  2:219731/1‑149 (MQ=255)
                                                                                              cTGCGGTTGCGGTTGTTCCTTTAGTATCTAATGTGTTGTTAATGTGCCGGATAAACTATCCGGCACATGTTGTCTCTTCAACGCCCCTTATCATCCAGCGCAAACGTCGGTAGCATAAGATGCCAACGAAAAGCCGCCAGCCGCTTCaa                                                       >  2:73141/1‑149 (MQ=255)
                                                                                                  ggttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGc                                                   <  1:298543/149‑1 (MQ=255)
                                                                                                    ttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGAACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGt                                                 <  2:805098/149‑1 (MQ=255)
                                                                                                      gcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCa                                               >  2:1239123/1‑149 (MQ=255)
                                                                                                      gcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCa                                               >  2:1280234/1‑149 (MQ=255)
                                                                                                        ggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACg                                             >  1:1225111/1‑149 (MQ=255)
                                                                                                        ggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCAGTCTCATCCAGCGCAAACGTCGGAAGCTTAAGATGCCACCGAAAACCCGCCAGCCGAATCAATAGCGTCCCg                                             >  2:1323709/1‑149 (MQ=255)
                                                                                                            cttcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCa                                         >  1:837382/1‑149 (MQ=255)
                                                                                                              tcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATg                                       <  1:31424/149‑1 (MQ=255)
                                                                                                              tcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATATTCTCTCTTCAACGACCATTCTCATCCAGCGAAAACGTCGGAAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATg                                       >  2:908642/1‑149 (MQ=255)
                                                                                                               ccTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGc                                      <  1:73141/149‑1 (MQ=255)
                                                                                                                cTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGcc                                     >  1:942861/1‑149 (MQ=255)
                                                                                                                  ttAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGccaa                                   >  1:1049834/1‑147 (MQ=255)
                                                                                                                  ttAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCca                                   <  2:821446/149‑1 (MQ=255)
                                                                                                                       aTCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCaa                                                                                                                           >  2:311381/1‑56 (MQ=255)
                                                                                                                       aTCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCaa                                                                                                                           <  1:311381/56‑1 (MQ=255)
                                                                                                                          tAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTg                           >  2:1099645/1‑149 (MQ=255)
                                                                                                                            aTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGc                         <  2:442802/149‑1 (MQ=255)
                                                                                                                            aTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCGTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCGGCCGAATCAATAGCGTCACGACCATGCCCATCATTCTGGc                         >  2:80421/1‑149 (MQ=255)
                                                                                                                             tCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCt                        >  2:258270/1‑149 (MQ=255)
                                                                                                                             tCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCt                        >  2:1243259/1‑149 (MQ=255)
                                                                                                                             tCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCt                        <  1:1374309/149‑1 (MQ=255)
                                                                                                                               tgttgtTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCGAACGCATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGt                      >  1:566596/1‑149 (MQ=255)
                                                                                                                               tgttgtTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGt                      >  1:922098/1‑149 (MQ=255)
                                                                                                                                  tgttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTc                   <  2:248448/149‑1 (MQ=255)
                                                                                                                                   gttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTcc                  >  2:1086777/1‑149 (MQ=255)
                                                                                                                                    ttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCa                 >  1:572196/1‑149 (MQ=255)
                                                                                                                                       aTGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTg              <  1:895785/149‑1 (MQ=255)
                                                                                                                                          tgCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTa           >  1:1466185/1‑149 (MQ=255)
                                                                                                                                            ccGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATATCCGCCAGCCGAATCAAAAGCGTCACGACCATGCCAATCAAAATTGATGTTTCAAGTGTTACg         >  2:287797/1‑149 (MQ=255)
                                                                                                                                             cGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGACACGACCATGCCAATCATACTGGCTGTATCCAGTGGTACgg        >  2:1215668/1‑149 (MQ=255)
                                                                                                                                               gATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaa      >  1:1078819/1‑149 (MQ=255)
                                                                                                                                                aTAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaaa     <  2:1371227/149‑1 (MQ=255)
                                                                                                                                                  aaaCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAt   <  2:354942/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATG  >  NC_000913/176830‑177125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: