Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,011,926 G→A 6.9% intergenic (+55/‑75) uup → / → pqiA replication regulatory ABC‑F family DNA‑binding ATPase/paraquat‑inducible, SoxRS‑regulated inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,011,9260GA 6.9% 266.3 / 7.0 87intergenic (+55/‑75)uup/pqiAreplication regulatory ABC‑F family DNA‑binding ATPase/paraquat‑inducible, SoxRS‑regulated inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (40/40);  new base A (4/2);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

AGTGCTTGCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCG  >  NC_000913/1011781‑1012071
                                                                                                                                                 |                                                                                                                                                 
aGTGCTTGCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAAc                                                                                                                                                <  1:1098704/149‑1 (MQ=255)
aGTGCTTGCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGGTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGAAAACGCTAAAATTGCCTGATGAAc                                                                                                                                                >  1:101819/1‑149 (MQ=255)
     ttgctGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGt                                                                                                                                           <  2:1461678/149‑1 (MQ=255)
       gctGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCTTTAAAAAATGGTGGCTGATCGCAATATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGttt                                                                                                                                         <  2:1034990/149‑1 (MQ=255)
         tGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTAt                                                                                                                                       <  2:925068/149‑1 (MQ=255)
           atatGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCa                                                                                                                                     >  2:1548663/1‑149 (MQ=255)
            tatGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAg                                                                                                                                    <  2:255451/149‑1 (MQ=255)
              tGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGc                                                                                                                                  <  2:479384/149‑1 (MQ=255)
               ggCTGCTGCAGTGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                 >  1:268604/1‑149 (MQ=255)
               ggCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                 <  1:485058/149‑1 (MQ=255)
               ggCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                 <  2:1504868/149‑1 (MQ=255)
               ggCTGCTGCAGAGCAGGAGCGGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATAAACCCAGTTCATCAGGCa                                                                                                                                 <  1:308273/149‑1 (MQ=255)
                gctgctGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCAt                                                                                                                                <  2:650330/149‑1 (MQ=255)
                gctgctGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATAAACCCAGTTCATCAGGCaa                                                                                                                                <  2:26475/149‑2 (MQ=255)
                  tgctgcAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGa                                                                                                                              >  1:1141843/1‑149 (MQ=255)
                     tgcAGAGCAGGAGCGGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGaaaa                                                                                                                           <  2:249325/149‑1 (MQ=255)
                       cAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAAcc                                                                                                                         >  2:249827/1‑149 (MQ=255)
                            cAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAacac                                                                                                                    >  2:1117078/1‑149 (MQ=255)
                                aGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTg                                                                                                                <  1:456645/149‑1 (MQ=255)
                                aGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGATAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTg                                                                                                                <  2:1140145/149‑1 (MQ=255)
                                 gCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGt                                                                                                               <  2:1410751/149‑1 (MQ=255)
                                  cTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGtt                                                                                                              <  2:1519196/149‑1 (MQ=255)
                                   tGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTa                                                                                                             <  1:1219791/149‑1 (MQ=255)
                                     gAGCAAGCCTTTGCACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAAGCATGAAAACCAACACATTCTTAGt                                                                                                           >  1:276828/1‑149 (MQ=255)
                                     gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                           >  1:1515034/1‑149 (MQ=255)
                                     gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                           >  1:1098237/1‑149 (MQ=255)
                                     gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                           >  2:174993/1‑149 (MQ=255)
                                     gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGAAAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                           >  2:798669/1‑149 (MQ=255)
                                       gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                         >  2:164499/1‑149 (MQ=255)
                                       gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                         <  1:1115008/149‑1 (MQ=255)
                                       gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                         >  1:457719/1‑149 (MQ=255)
                                       gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                         >  2:322009/1‑149 (MQ=255)
                                        cAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCAt                                                                                                        >  2:120149/1‑149 (MQ=255)
                                              tttGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATTAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTaa                                                                                                  <  1:914042/149‑1 (MQ=255)
                                              tttGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTaa                                                                                                  >  1:166727/1‑149 (MQ=255)
                                                    cGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTTCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAgc                                                                                            <  1:1155533/149‑1 (MQ=255)
                                                       tGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTATAATAGCGca                                                                                         >  2:1050909/1‑149 (MQ=255)
                                                         ggAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCGTCTGTAAAATAGCGcatc                                                                                       >  1:632278/1‑149 (MQ=255)
                                                            gTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCAtt                                                                                    >  2:197605/1‑149 (MQ=255)
                                                             tATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACATAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGATTATAAGGCATGAAAACCAACACTTTGTTAGACATCTGTAAAATACCGCATCATTa                                                                                   >  2:783548/1‑149 (MQ=255)
                                                                   gAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCTTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGt                                                                             >  2:1238315/1‑149 (MQ=255)
                                                                     aGCGTTAAAAAATGGTGGCTGATCACAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGGTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTAc                                                                           <  1:976734/149‑1 (MQ=255)
                                                                         ttAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                       >  2:584442/1‑149 (MQ=255)
                                                                         ttAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAAAAGCTCATCATTAAGGAGTACCAAt                                                                       >  1:779796/1‑149 (MQ=255)
                                                                                aTGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACTGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGaa                                                                <  1:174993/149‑1 (MQ=255)
                                                                                aTGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGaa                                                                >  2:575680/1‑149 (MQ=255)
                                                                                      gCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAAcatcat                                                          <  2:1515034/149‑1 (MQ=255)
                                                                                      gCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAAcatcat                                                          <  2:1098237/149‑1 (MQ=255)
                                                                                        tGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                       >  1:703973/1‑134 (MQ=255)
                                                                                        tGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                       <  2:703973/134‑1 (MQ=255)
                                                                                            ctaaaaaTATTATATTTAAACAGACACAGCTGACAACGGTAAAATTGCCTGATGAACTTGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGcc                                                    <  1:1419173/146‑1 (MQ=255)
                                                                                                     gTCGATTTAAACAGATACAGCTTAAAACTGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAATcac                                           <  1:680596/149‑1 (MQ=255)
                                                                                                      tCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcaca                                          >  2:1571464/1‑149 (MQ=255)
                                                                                                      tCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcaca                                          >  1:934832/1‑149 (MQ=255)
                                                                                                          tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcacaggat                                      <  1:164499/149‑5 (MQ=255)
                                                                                                          tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCt                                      >  1:819315/1‑149 (MQ=255)
                                                                                                          tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACAAAATCATGCCGCGAATCACATcat                                      >  1:187827/1‑147 (MQ=255)
                                                                                                          tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCAGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCt                                      >  1:835477/1‑149 (MQ=255)
                                                                                                           ttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCtg                                     <  1:120149/149‑1 (MQ=255)
                                                                                                              aaCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGc                                  >  2:13627/1‑149 (MQ=255)
                                                                                                              aaCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGc                                  <  1:575680/149‑1 (MQ=255)
                                                                                                               aCAGAGACAGCTGACACCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                 <  2:300214/149‑1 (MQ=255)
                                                                                                               aCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                 >  1:929386/1‑149 (MQ=255)
                                                                                                               aCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACAAACACTTTGTTAGTCATCTGTATAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                 >  2:981920/1‑149 (MQ=255)
                                                                                                                   agaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACAAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCa                             >  2:1180689/1‑149 (MQ=255)
                                                                                                                    gaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCGTCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAGCACAGGATGTGCttcgcgg                           <  2:632278/149‑7 (MQ=255)
                                                                                                                    gaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAg                            <  2:268604/149‑1 (MQ=255)
                                                                                                                     aCAGCTGACAACGGTAAAATTGCCTGATGAACTGGTTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgt                           <  2:1415159/149‑1 (MQ=255)
                                                                                                                     aCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgt                           <  2:457719/149‑1 (MQ=255)
                                                                                                                       aGCTGACAACGGTAAATTTGCCTTATGAACTGGGTTTATCAGGCATGAAAACCTACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgtgt                         <  1:624804/149‑1 (MQ=255)
                                                                                                                         cTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGa                       >  1:653360/1‑149 (MQ=255)
                                                                                                                          tGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGAc                      <  2:653360/149‑1 (MQ=255)
                                                                                                                          tGACAACGGTAAAATTGCCTGATGAACTGGGTTTATAAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCAACATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGAc                      >  1:1090923/1‑149 (MQ=255)
                                                                                                                             cAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATg                   <  1:1117078/149‑1 (MQ=255)
                                                                                                                              aaCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGc                  <  2:160149/149‑1 (MQ=255)
                                                                                                                               aCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCt                 <  1:1548663/149‑1 (MQ=255)
                                                                                                                                  gTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGACGCACATCCTGTGCTCGCAGTGTGACATGCtggt              >  1:760096/1‑149 (MQ=255)
                                                                                                                                  gTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTGTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAATCACATCGTGTGCTCGCAGTGTGACATGCtggt              >  1:295321/1‑149 (MQ=255)
                                                                                                                                   ttttcatGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCtggtg             >  2:803410/7‑149 (MQ=255)
                                                                                                                                     aaaTTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGc           <  2:166727/149‑1 (MQ=255)
                                                                                                                                       aTTGCTTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGt         <  1:1238315/149‑1 (MQ=255)
                                                                                                                                         tGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa       <  2:1411621/149‑1 (MQ=255)
                                                                                                                                         tGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa       >  2:582727/1‑149 (MQ=255)
                                                                                                                                         tGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa       >  1:1080471/1‑149 (MQ=255)
                                                                                                                                          gCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTAc      >  1:787935/1‑149 (MQ=255)
                                                                                                                                           ccTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTAcc     >  1:418909/1‑149 (MQ=255)
                                                                                                                                            cTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcg    >  2:1067014/1‑149 (MQ=255)
                                                                                                                                             tgatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgc   >  1:1541180/1‑149 (MQ=255)
                                                                                                                                              gatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgcg  >  1:1199854/1‑149 (MQ=255)
                                                                                                                                              gatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgcg  >  1:1024141/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGTGCTTGCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCG  >  NC_000913/1011781‑1012071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: