Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 208,552 T→A 9.7% L1143I (TTA→ATA)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913208,5520TA 9.7% 309.6 / 18.7 111L1143I (TTA→ATA) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (50/49);  new base A (8/3);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

Only 100 of 115 total aligned reads displayed.

ATGACCAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTAGCC  >  NC_000913/208406‑208699
                                                                                                                                                  |                                                                                                                                                   
atgaCCAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTTTCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTttatta                                                                                                                                                   >  2:228824/1‑149 (MQ=255)
  gaCCAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTaaa                                                                                                                                                 <  1:690051/149‑1 (MQ=255)
   aCCAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAAc                                                                                                                                                <  2:353733/149‑1 (MQ=255)
    ccAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACg                                                                                                                                               >  1:965160/1‑149 (MQ=255)
    ccAGCTTTTAAACCGACTCCGTCAGGCTCTGGAACCCCACCGCTCTGGGCCAATACCAGTACATCTCGACTAAAAGAAGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGAGACTTGGCTGGTCTCTCCTAGCGATCGATTATAAAACg                                                                                                                                               >  2:259498/1‑149 (MQ=255)
     cAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGa                                                                                                                                              >  1:28572/1‑149 (MQ=255)
       gCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTAATAAACGAtc                                                                                                                                            <  2:928931/149‑1 (MQ=255)
       gCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTAGCGTTTTGGCGCGACGTGCCGTGTCTCTCCGCGCGATCGTTTATTAAACGAtc                                                                                                                                            >  1:195748/1‑149 (MQ=255)
        cTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTAATAAACGAtcg                                                                                                                                           <  1:93501/149‑2 (MQ=255)
          tttAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTcc                                                                                                                                         >  1:1363818/1‑149 (MQ=255)
          tttAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTcc                                                                                                                                         >  1:987241/1‑149 (MQ=255)
          tttAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTcc                                                                                                                                         <  1:281306/149‑1 (MQ=255)
          tttAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTATTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTATATTAAACGATCTcc                                                                                                                                         >  2:745903/1‑149 (MQ=255)
            tAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTAATAAACGAtcgctcg                                                                                                                                       <  2:498076/149‑6 (MQ=255)
                ccGACTCCGTCAGTCTCTGGAACCCCACCGCTCTTGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCTACGTGGCGTGTCTCTCCTATCGATCGTTTATTAAAAGATCTCCGtcgac                                                                                                                                   >  2:128212/1‑145 (MQ=255)
                ccGACTCCGTCAGTCTCTGGAACACCACCGCTCTGGGACAATTCCAGAACATCTCTACTAGCAGAGGGCGGATGCACGCGCGCGGGAGCGTTTTGGCGCGACGTGGCGGGTCTCTCCGAGCGATCGTTTAGTAAACGATCTCCGTGGcc                                                                                                                                   >  1:1121554/1‑149 (MQ=255)
                            gTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCgg                                                                                                                       >  2:1383892/1‑149 (MQ=255)
                            gTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCgg                                                                                                                       <  1:1341031/149‑1 (MQ=255)
                             tctcTGGAACCCCACCGCTCTGGGACTATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGa                                                                                                                      <  1:125845/149‑1 (MQ=255)
                             tctcTGGAACCACACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGGCGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCAGTGGTTCggt                                                                                                                      >  1:563196/1‑148 (MQ=255)
                               tctGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGc                                                                                                                    >  1:1564828/1‑149 (MQ=255)
                               tctGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGc                                                                                                                    >  2:348352/1‑149 (MQ=255)
                                 tGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAg                                                                                                                  <  2:1480273/149‑1 (MQ=255)
                                    aaCCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTg                                                                                                               <  1:608161/149‑1 (MQ=255)
                                    aaCCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTg                                                                                                               <  1:923924/149‑1 (MQ=255)
                                    aaCCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTg                                                                                                               >  1:1597084/1‑149 (MQ=255)
                                      ccccACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGCTCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGa                                                                                                             >  2:192160/1‑149 (MQ=255)
                                      ccccACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGa                                                                                                             >  2:931375/1‑149 (MQ=255)
                                       cccACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGaa                                                                                                            >  1:1494119/1‑149 (MQ=255)
                                        ccACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAAc                                                                                                           <  2:1599523/149‑1 (MQ=255)
                                         cACCGCTCTGGGACAATTCCTGTACATCTCTACTATCAGTGGGCGGATGCAGGCTCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACt                                                                                                          <  1:370145/149‑1 (MQ=255)
                                                ctGGGACAATACCAGCACATCTCAACTATCCGAGGGCGGATGCACGCGCGCGGGGGCGGTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGttt                                                                                                   <  2:769856/149‑1 (MQ=255)
                                                  gggACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTTCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGa                                                                                                 <  2:927009/149‑1 (MQ=255)
                                                  gggACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGa                                                                                                 <  2:1593965/149‑1 (MQ=255)
                                                    gACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGCTCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACt                                                                                               >  1:964908/1‑149 (MQ=255)
                                                    gACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACt                                                                                               >  1:1185500/1‑149 (MQ=255)
                                                       aaTTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAAt                                                                                            >  1:443332/1‑149 (MQ=255)
                                                        aTTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATa                                                                                           >  1:606776/1‑149 (MQ=255)
                                                         ttCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATAc                                                                                          >  2:601417/1‑149 (MQ=255)
                                                          tCCTGTACATCTCTACTATCTGAGGGCGGATGAACGAGCGCGGTGGCGTTTTGGCGCGTCGTGGCGTGTCTCTCCGAGCGATGGTTTATTAAAGGATCTCCGTGGCCTCATTGGTTCGGAGCAGGGGGAACTGGTGTTTGACTTATACa                                                                                         <  2:619822/149‑1 (MQ=255)
                                                          tCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACa                                                                                         >  1:1440425/1‑149 (MQ=255)
                                                            cAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACa                                                                                         <  2:930072/147‑1 (MQ=255)
                                                            cAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAgg                                                                                       >  1:109757/1‑149 (MQ=255)
                                                             aGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGa                                                                                      >  2:71503/1‑149 (MQ=255)
                                                               tACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGAt                                                                                                                                             >  2:279924/1‑92 (MQ=255)
                                                               tACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGAt                                                                                                                                             <  1:279924/92‑1 (MQ=255)
                                                               tACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGaaa                                                                                    >  2:1343660/1‑148 (MQ=255)
                                                                  aTCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGAc                                                                                                <  2:780686/134‑1 (MQ=255)
                                                                  aTCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGAc                                                                                                >  1:780686/1‑134 (MQ=255)
                                                                    ctctCCTATCAGAGGGCGGATGCACGCGCGCGGTGGCGGTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTTTTAAACGATCTCCGTGGCCTCATTGGATCGGAGCAGGTGTAACTGGAGTTTGACTAATACAGGAATACTAt                                                                               <  1:763077/149‑1 (MQ=255)
                                                                    ctctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACTATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTAt                                                                               <  2:442036/149‑1 (MQ=255)
                                                                    ctctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTAt                                                                               <  2:1171854/149‑1 (MQ=255)
                                                                    ctctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTAt                                                                               >  1:665851/1‑149 (MQ=255)
                                                                     tctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATg                                                                              >  2:32996/1‑149 (MQ=255)
                                                                     tctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATg                                                                              >  1:692854/1‑149 (MQ=255)
                                                                     tctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATg                                                                              >  1:124416/1‑149 (MQ=255)
                                                                     tctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATg                                                                              >  2:1321040/1‑149 (MQ=255)
                                                                     tctACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGAACGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACAGGAGTTTGACTAATACAGGAATACTATg                                                                              >  2:360008/1‑149 (MQ=255)
                                                                          taTCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCt                                                                         >  2:1362196/1‑149 (MQ=255)
                                                                           aTCTGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGAGCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGGTTGACTAATACAGGAATACTATGAGTCTg                                                                        <  1:904204/149‑1 (MQ=255)
                                                                              agagGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAAt                                                                     >  2:463193/1‑149 (MQ=255)
                                                                              agagGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAAt                                                                     <  1:1051778/149‑1 (MQ=255)
                                                                               gagGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAAtt                                                                    >  2:583152/1‑149 (MQ=255)
                                                                                 gggCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTc                                                                  <  2:1363818/149‑1 (MQ=255)
                                                                                  ggCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGTAACTTGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTcc                                                                 <  2:1574421/149‑1 (MQ=255)
                                                                                  ggCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTcc                                                                 <  1:890138/149‑1 (MQ=255)
                                                                                        tGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGAtt                                                           <  1:51264/149‑1 (MQ=255)
                                                                                           aCGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCTATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATAGTATGAGTCTGAATTTCCTTGATTTTg                                                        <  2:801910/149‑1 (MQ=255)
                                                                                            cgcgcgcgGGTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAATCGATCTACGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGa                                                       <  2:845625/149‑1 (MQ=255)
                                                                                                cgcgGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAg                                                   <  1:1383892/149‑1 (MQ=255)
                                                                                                  cgGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGcc                                                 <  1:57309/149‑1 (MQ=255)
                                                                                                  cgGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGcc                                                 <  1:589252/149‑1 (MQ=255)
                                                                                                      tGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGAtt                                             <  2:195748/149‑1 (MQ=255)
                                                                                                      tGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCGCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGAtt                                             <  2:1108762/149‑1 (MQ=255)
                                                                                                                cgcgACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTgg                                   >  1:91315/1‑149 (MQ=255)
                                                                                                                  cgACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGaa                                 <  2:1564828/149‑1 (MQ=255)
                                                                                                                        ggCGTGTCTATCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCTGAGCAGGTGTAACTGGATTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCAGATTGCAGAGCTGGAAGCGaaa                           <  1:784701/149‑1 (MQ=255)
                                                                                                                           gtgtCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGTGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCATTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATc                        >  2:1459522/1‑149 (MQ=255)
                                                                                                                             gtCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGa                      <  2:964908/149‑1 (MQ=255)
                                                                                                                               ctctcCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGAtt                    >  1:1530424/1‑149 (MQ=255)
                                                                                                                               ctctcCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGAtt                    >  2:1397694/1‑149 (MQ=255)
                                                                                                                               ctctcCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGAtt                    >  1:177036/1‑149 (MQ=255)
                                                                                                                                  tcCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAAAACAGGAATACTATGAGTCTGAATTTCCATGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATtctc                 >  1:1222606/1‑149 (MQ=255)
                                                                                                                                   cacggagATCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTctct                >  2:1372767/7‑149 (MQ=255)
                                                                                                                                      ggagATCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGAc             >  1:885489/4‑149 (MQ=255)
                                                                                                                                       gCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACt            <  2:692854/149‑1 (MQ=255)
                                                                                                                                        agATCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTg           >  2:1063285/2‑149 (MQ=255)
                                                                                                                                        agATCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTg           >  2:367951/2‑149 (MQ=255)
                                                                                                                                         gATCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCAAAAATCGATTCTCTGACTGc          >  2:280475/1‑149 (MQ=255)
                                                                                                                                          atgttttATTAAAGGATCTCGGTGGCCTCATTGGTTCGGAGCAGGTGGAAGTGGAGTTTGACTAATACAGTAATCCTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGTTTGCAGAGCTGGAAGCGAAAATGGATTCTCTGACTGCg         <  1:128212/145‑1 (MQ=255)
                                                                                                                                          aTCGTTTAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGATCTGGAGTTTGACTAATACAGGAATAGTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCACAGCTGGAAGCGAAAATCGATTCTCTGACTGca         >  1:299993/1‑148 (MQ=255)
                                                                                                                                           tCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCgg        >  1:1562177/1‑149 (MQ=255)
                                                                                                                                            cGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGTAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGt       <  2:965160/149‑1 (MQ=255)
                                                                                                                                             gTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGtt      >  2:353858/1‑149 (MQ=255)
                                                                                                                                             gTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTCGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGtt      >  1:641444/1‑149 (MQ=255)
                                                                                                                                              tttATTAAACGATCTCCGTTGCCTCATTGGTTCTGAGCATTTTTAACTTTAGTTTGACTAATACAGTAATACTATTAGTCTGAATTTCCTTTATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTa     <  1:676613/149‑1 (MQ=255)
                                                                                                                                              tttAATAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTa     >  2:556455/1‑149 (MQ=255)
                                                                                                                                                tattaAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTAGc   <  1:520185/149‑1 (MQ=255)
                                                                                                                                                 attaAACTCTCTCCGTTGCCTCCTTGGTTCGGAGCTGGGGGTACTGGAGTTTGCGTAATACAGGAATACTATGAGTCTGAATTTCGTTGATTTTGAACAGCCGATTGCAGAGCTGGATGAGAAAATCGATTCTCTGACTGCGGTTAGcc  <  1:259498/149‑1 (MQ=255)
                                                                                                                                                 attaAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTAGcc  <  1:1397694/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATGACCAGCTTTTAAACCGACTCCGTCAGTCTCTGGAACCCCACCGCTCTGGGACAATTCCAGTACATCTCTACTATCAGAGGGCGGATGCACGCGCGCGGTTGCGTTTTGGCGCGACGTGGCGTGTCTCTCCGAGCGATCGTTTATTAAACGATCTCCGTGGCCTCATTGGTTCGGAGCAGGTGGAACTGGAGTTTGACTAATACAGGAATACTATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGATTCTCTGACTGCGGTTAGCC  >  NC_000913/208406‑208699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: