Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,011,936 T→C 6.6% intergenic (+65/‑65) uup → / → pqiA replication regulatory ABC‑F family DNA‑binding ATPase/paraquat‑inducible, SoxRS‑regulated inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,011,9360TC 6.6% 278.4 / 7.6 91intergenic (+65/‑65)uup/pqiAreplication regulatory ABC‑F family DNA‑binding ATPase/paraquat‑inducible, SoxRS‑regulated inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (44/41);  new base C (4/2);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

GCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATGGT  >  NC_000913/1011788‑1012084
                                                                                                                                                    |                                                                                                                                                    
gctGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCTTTAAAAAATGGTGGCTGATCGCAATATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGttt                                                                                                                                                      <  2:1034990/149‑1 (MQ=255)
  tGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTAt                                                                                                                                                    <  2:925068/149‑1 (MQ=255)
    atatGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCa                                                                                                                                                  >  2:1548663/1‑149 (MQ=255)
     tatGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAg                                                                                                                                                 <  2:255451/149‑1 (MQ=255)
       tGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGc                                                                                                                                               <  2:479384/149‑1 (MQ=255)
        ggCTGCTGCAGTGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                              >  1:268604/1‑149 (MQ=255)
        ggCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                              <  1:485058/149‑1 (MQ=255)
        ggCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCa                                                                                                                                              <  2:1504868/149‑1 (MQ=255)
        ggCTGCTGCAGAGCAGGAGCGGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATAAACCCAGTTCATCAGGCa                                                                                                                                              <  1:308273/149‑1 (MQ=255)
         gctgctGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCAt                                                                                                                                             <  2:650330/149‑1 (MQ=255)
         gctgctGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATAAACCCAGTTCATCAGGCaa                                                                                                                                             <  2:26475/149‑2 (MQ=255)
           tgctgcAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGa                                                                                                                                           >  1:1141843/1‑149 (MQ=255)
              tgcAGAGCAGGAGCGGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGaaaa                                                                                                                                        <  2:249325/149‑1 (MQ=255)
                cAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAAcc                                                                                                                                      >  2:249827/1‑149 (MQ=255)
                     cAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAacac                                                                                                                                 >  2:1117078/1‑149 (MQ=255)
                         aGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTg                                                                                                                             <  1:456645/149‑1 (MQ=255)
                         aGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGATAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTg                                                                                                                             <  2:1140145/149‑1 (MQ=255)
                          gCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGt                                                                                                                            <  2:1410751/149‑1 (MQ=255)
                           cTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGtt                                                                                                                           <  2:1519196/149‑1 (MQ=255)
                            tGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTa                                                                                                                          <  1:1219791/149‑1 (MQ=255)
                              gAGCAAGCCTTTGCACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAAGCATGAAAACCAACACATTCTTAGt                                                                                                                        >  1:276828/1‑149 (MQ=255)
                              gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                                        >  1:1515034/1‑149 (MQ=255)
                              gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                                        >  1:1098237/1‑149 (MQ=255)
                              gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                                        >  2:174993/1‑149 (MQ=255)
                              gAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGAAAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGt                                                                                                                        >  2:798669/1‑149 (MQ=255)
                                gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                                      >  1:457719/1‑149 (MQ=255)
                                gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                                      >  2:322009/1‑149 (MQ=255)
                                gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                                      >  2:164499/1‑149 (MQ=255)
                                gCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCa                                                                                                                      <  1:1115008/149‑1 (MQ=255)
                                 cAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCAt                                                                                                                     >  2:120149/1‑149 (MQ=255)
                                       tttGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATTAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTaa                                                                                                               <  1:914042/149‑1 (MQ=255)
                                       tttGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTaa                                                                                                               >  1:166727/1‑149 (MQ=255)
                                             cGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTTCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAgc                                                                                                         <  1:1155533/149‑1 (MQ=255)
                                                tGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTATAATAGCGca                                                                                                      >  2:1050909/1‑149 (MQ=255)
                                                  ggAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCGTCTGTAAAATAGCGcatc                                                                                                    >  1:632278/1‑149 (MQ=255)
                                                     gTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCAtt                                                                                                 >  2:197605/1‑149 (MQ=255)
                                                      tATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACATAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGATTATAAGGCATGAAAACCAACACTTTGTTAGACATCTGTAAAATACCGCATCATTa                                                                                                >  2:783548/1‑149 (MQ=255)
                                                            gAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCTTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGt                                                                                          >  2:1238315/1‑149 (MQ=255)
                                                              aGCGTTAAAAAATGGTGGCTGATCACAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGGTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTAc                                                                                        <  1:976734/149‑1 (MQ=255)
                                                                  ttAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                                    >  2:584442/1‑149 (MQ=255)
                                                                  ttAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAAAAGCTCATCATTAAGGAGTACCAAt                                                                                    >  1:779796/1‑149 (MQ=255)
                                                                         aTGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACTGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGaa                                                                             <  1:174993/149‑1 (MQ=255)
                                                                         aTGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGaa                                                                             >  2:575680/1‑149 (MQ=255)
                                                                               gCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAAcatcat                                                                       <  2:1515034/149‑1 (MQ=255)
                                                                               gCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAAcatcat                                                                       <  2:1098237/149‑1 (MQ=255)
                                                                                 tGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                                    >  1:703973/1‑134 (MQ=255)
                                                                                 tGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAAt                                                                                    <  2:703973/134‑1 (MQ=255)
                                                                                     ctaaaaaTATTATATTTAAACAGACACAGCTGACAACGGTAAAATTGCCTGATGAACTTGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGcc                                                                 <  1:1419173/146‑1 (MQ=255)
                                                                                              gTCGATTTAAACAGATACAGCTTAAAACTGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAATcac                                                        <  1:680596/149‑1 (MQ=255)
                                                                                               tCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcaca                                                       >  2:1571464/1‑149 (MQ=255)
                                                                                               tCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcaca                                                       >  1:934832/1‑149 (MQ=255)
                                                                                                   tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGcacaggat                                                   <  1:164499/149‑5 (MQ=255)
                                                                                                   tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCt                                                   >  1:819315/1‑149 (MQ=255)
                                                                                                   tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACAAAATCATGCCGCGAATCACATcat                                                   >  1:187827/1‑147 (MQ=255)
                                                                                                   tttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCAGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCt                                                   >  1:835477/1‑149 (MQ=255)
                                                                                                    ttAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCtg                                                  <  1:120149/149‑1 (MQ=255)
                                                                                                       aaCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGc                                               >  2:13627/1‑149 (MQ=255)
                                                                                                       aaCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGc                                               <  1:575680/149‑1 (MQ=255)
                                                                                                        aCAGAGACAGCTGACACCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                              <  2:300214/149‑1 (MQ=255)
                                                                                                        aCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                              >  1:929386/1‑149 (MQ=255)
                                                                                                        aCAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACAAACACTTTGTTAGTCATCTGTATAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCt                                              >  2:981920/1‑149 (MQ=255)
                                                                                                            agaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACAAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCa                                          >  2:1180689/1‑149 (MQ=255)
                                                                                                             gaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCGTCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAGCACAGGATGTGCttcgcgg                                        <  2:632278/149‑7 (MQ=255)
                                                                                                             gaCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAg                                         <  2:268604/149‑1 (MQ=255)
                                                                                                              aCAGCTGACAACGGTAAAATTGCCTGATGAACTGGTTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgt                                        <  2:1415159/149‑1 (MQ=255)
                                                                                                              aCAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgt                                        <  2:457719/149‑1 (MQ=255)
                                                                                                                aGCTGACAACGGTAAATTTGCCTTATGAACTGGGTTTATCAGGCATGAAAACCTACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAgtgt                                      <  1:624804/149‑1 (MQ=255)
                                                                                                                  cTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGa                                    >  1:653360/1‑149 (MQ=255)
                                                                                                                   tGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGAc                                   <  2:653360/149‑1 (MQ=255)
                                                                                                                   tGACAACGGTAAAATTGCCTGATGAACTGGGTTTATAAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCAACATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGAc                                   >  1:1090923/1‑149 (MQ=255)
                                                                                                                      cAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATg                                <  1:1117078/149‑1 (MQ=255)
                                                                                                                       aaCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGc                               <  2:160149/149‑1 (MQ=255)
                                                                                                                        aCGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCt                              <  1:1548663/149‑1 (MQ=255)
                                                                                                                           gTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGACGCACATCCTGTGCTCGCAGTGTGACATGCtggt                           >  1:760096/1‑149 (MQ=255)
                                                                                                                           gTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTGTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAATCACATCGTGTGCTCGCAGTGTGACATGCtggt                           >  1:295321/1‑149 (MQ=255)
                                                                                                                            ttttcatGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCtggtg                          >  2:803410/7‑149 (MQ=255)
                                                                                                                              aaaTTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGc                        <  2:166727/149‑1 (MQ=255)
                                                                                                                                aTTGCTTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGt                      <  1:1238315/149‑1 (MQ=255)
                                                                                                                                  tGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa                    <  2:1411621/149‑1 (MQ=255)
                                                                                                                                  tGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa                    >  1:1080471/1‑149 (MQ=255)
                                                                                                                                  tGCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTa                    >  2:582727/1‑149 (MQ=255)
                                                                                                                                   gCCTGATAAACCCAGTTCATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTAc                   >  1:787935/1‑149 (MQ=255)
                                                                                                                                    ccTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTAcc                  >  1:418909/1‑149 (MQ=255)
                                                                                                                                     cTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcg                 >  2:1067014/1‑149 (MQ=255)
                                                                                                                                      tgatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgc                >  1:1541180/1‑149 (MQ=255)
                                                                                                                                       gatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgcg               >  1:1199854/1‑149 (MQ=255)
                                                                                                                                       gatgaACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACcgcg               >  1:1024141/1‑149 (MQ=255)
                                                                                                                                             cTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGa         <  1:322009/149‑1 (MQ=255)
                                                                                                                                             cTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGa         <  2:929386/149‑1 (MQ=255)
                                                                                                                                                ggTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCa      >  2:7469/1‑149 (MQ=255)
                                                                                                                                                ggTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCa      >  1:373137/1‑149 (MQ=255)
                                                                                                                                                 gTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCAt     <  2:934832/149‑1 (MQ=255)
                                                                                                                                                  tttATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATg    <  2:819315/149‑1 (MQ=255)
                                                                                                                                                  tttATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATg    <  2:841247/149‑1 (MQ=255)
                                                                                                                                                  tttATCAGGCATGAAAACCAACACTTTGTTAGTCATCCGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATg    <  2:835477/149‑1 (MQ=255)
                                                                                                                                                    tATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCAACATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATGGt  <  1:873958/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGATATGGCTGCTGCAGAGCAGGAGCTGGAGCAAGCCTTTGAACGCTGGGAGTATCTTGAAGCGTTAAAAAATGGTGGCTGATCGCAAAATAGTCGATTTAAACAGAGACAGCTGACAACGGTAAAATTGCCTGATGAACTGGGTTTATCAGGCATGAAAACCAACACTTTGTTAGTCATCTGTAAAATAGCGCATCATTAAGGAGTACCAATGTGCGAACATCATCATGCCGCGAAGCACATCCTGTGCTCGCAGTGTGACATGCTGGTGGCGTTACCGCGCCTTGAGCATGGT  >  NC_000913/1011788‑1012084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: