Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,030,720 C→T 5.4% intergenic (‑52/+39) tusE ← / ← yccA mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,030,7200CT 5.4% 407.9 / 8.1 130intergenic (‑52/+39)tusE/yccAmnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (65/56);  new base T (5/2);  total (72/58)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-02

Only 100 of 133 total aligned reads displayed.

TGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAA  >  NC_000913/1030573‑1030868
                                                                                                                                                   |                                                                                                                                                    
tGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGcccc                                                                                                                                                     >  2:25319/1‑149 (MQ=255)
  cAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTTTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGAGGCAGTGTAACAAAAAACAAAGCACCGc                                                                                                                                                   >  2:319124/1‑149 (MQ=255)
    aTCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCAt                                                                                                                                                 >  2:1353728/1‑149 (MQ=255)
     tCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATa                                                                                                                                                >  1:826994/1‑149 (MQ=255)
        ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAATATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAg                                                                                                                                             >  2:112273/1‑149 (MQ=255)
        ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAg                                                                                                                                             >  2:465787/1‑149 (MQ=255)
         aacaGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGc                                                                                                                                            >  2:1472581/2‑149 (MQ=255)
              ccagtGTCTAACTACACTGGCTGCTTTCTTTTTGATAGCCTTCGGTATCCGTTTCTATCTCATTACCTTAGAATATCATCAAAATTATTACAACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                       <  2:310142/149‑1 (MQ=255)
              ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTAACCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                       >  1:937398/1‑149 (MQ=255)
              ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCATCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTATAAGCGGGGc                                                                                                                                       >  1:190578/1‑149 (MQ=255)
                   ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgt                                                                                                                                  <  1:1464462/149‑2 (MQ=255)
                        aCTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgtttttt                                                                                                                             <  2:1207730/149‑7 (MQ=255)
                         cTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAGAACAAAGCCCCGCATAAAGCGAGGCTATGAAAgtgt                                                                                                                            <  1:1159606/149‑1 (MQ=255)
                          tCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGtt                                                                                                                           >  1:343202/1‑149 (MQ=255)
                            cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                         >  1:1531298/1‑149 (MQ=255)
                                 gctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGt                                                                                                                    <  2:937398/149‑1 (MQ=255)
                                  ctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTg                                                                                                                   <  1:1701/149‑1 (MQ=255)
                                    gctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgag                                                                                                                 >  1:1424169/1‑149 (MQ=255)
                                     ctttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgaga                                                                                                                <  1:121423/149‑1 (MQ=255)
                                      tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                               >  2:1484781/1‑149 (MQ=255)
                                      tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                               <  1:763054/149‑1 (MQ=255)
                                      tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGACAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                               >  1:1224895/1‑149 (MQ=255)
                                          tttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAAt                                                                                                           <  1:1182248/149‑1 (MQ=255)
                                             gagaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgc                                                                                                        >  2:1368993/1‑149 (MQ=255)
                                              agaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgcg                                                                                                       <  2:504764/149‑1 (MQ=255)
                                                aTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGc                                                                                                     <  1:1143368/149‑1 (MQ=255)
                                                 tAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCt                                                                                                    >  2:1434972/1‑149 (MQ=255)
                                                    ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                 >  2:139382/1‑149 (MQ=255)
                                                    ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                 >  2:1312279/1‑149 (MQ=255)
                                                    ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                 >  1:1107635/1‑149 (MQ=255)
                                                    ccTTCGGTATCCGTTTCTACCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                 >  2:927255/1‑149 (MQ=255)
                                                      ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                               >  1:377591/1‑149 (MQ=255)
                                                      ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                               >  1:378103/1‑149 (MQ=255)
                                                       tCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGaa                                                                                              <  2:1142336/149‑1 (MQ=255)
                                                        cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                             <  1:280929/149‑1 (MQ=255)
                                                        cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                             >  2:389684/1‑149 (MQ=255)
                                                         ggTATCAGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTTGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGc                                                                                            <  2:842983/149‑1 (MQ=255)
                                                          gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTTGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAATCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                           <  2:57474/149‑1 (MQ=255)
                                                          gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                           <  1:525394/149‑1 (MQ=255)
                                                            aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                         <  1:1250670/149‑1 (MQ=255)
                                                               cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                      <  2:638021/149‑1 (MQ=255)
                                                               cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                      >  1:1438923/1‑149 (MQ=255)
                                                                   tCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCt                                                                                  <  1:1368993/149‑1 (MQ=255)
                                                                     tATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGAGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATAGCGGCTAGCGAAGCCCAGAATGCTca                                                                                >  2:902819/1‑149 (MQ=255)
                                                                     tATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGGGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTca                                                                                <  1:1503375/149‑1 (MQ=255)
                                                                      aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                               >  2:727147/1‑149 (MQ=255)
                                                                      aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                               >  1:937899/1‑149 (MQ=255)
                                                                      aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                               <  1:1298104/149‑1 (MQ=255)
                                                                           tttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGc                                                                          >  2:524018/1‑149 (MQ=255)
                                                                               ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                      >  2:1347028/1‑149 (MQ=255)
                                                                               ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                      <  1:1347028/149‑1 (MQ=255)
                                                                               ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                      >  2:1067233/1‑149 (MQ=255)
                                                                               ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                      >  2:109961/1‑149 (MQ=255)
                                                                                cTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACg                                                                     <  2:1424169/149‑1 (MQ=255)
                                                                                 ttCGAAGATCAGCATAATTATTACTACATTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACTCATAAAGCGAGTCTATGAAAGTGTTAGAGGGTGAGATTAATCGCGGCTAGCTAAGCCCAGAATGCTCAGCAGGCTGACGa                                                                    >  1:124956/1‑149 (MQ=255)
                                                                                     aaGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAt                                                                >  1:713925/1‑149 (MQ=255)
                                                                                      aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                               <  2:746709/149‑1 (MQ=255)
                                                                                      aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                               >  1:192261/1‑149 (MQ=255)
                                                                                      aGATAAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                               <  2:4790/149‑1 (MQ=255)
                                                                                       gATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgt                                                              <  1:109961/149‑1 (MQ=255)
                                                                                         tCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgttg                                                            <  2:280178/149‑1 (MQ=255)
                                                                                           aGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTa                                                          >  1:754127/1‑149 (MQ=255)
                                                                                            gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                         <  1:901630/149‑1 (MQ=255)
                                                                                            gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                         >  1:1333798/1‑149 (MQ=255)
                                                                                               tAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGc                                                      >  2:603686/1‑149 (MQ=255)
                                                                                                 attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCTGGGTGACGATGATGTTGTACAGCGa                                                    >  2:1039137/1‑149 (MQ=255)
                                                                                                 attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGAGAGATTAATCGCGGCTAGCGAAGCCCAGAAAGCTCAGCAGGATCACGCAGATGTTGTTCAGCGa                                                    >  1:328978/1‑149 (MQ=255)
                                                                                                  ttattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAACCCCAGAATGCACAGCAGGCAGACGAAGATGTTGTACAGTGaa                                                   >  2:1068722/1‑149 (MQ=255)
                                                                                                   tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                  <  2:1369369/149‑1 (MQ=255)
                                                                                                        ctacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATAc                                             >  1:453638/1‑149 (MQ=255)
                                                                                                         tacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACa                                            >  1:84855/1‑149 (MQ=255)
                                                                                                          acCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCTAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAg                                           <  1:1240820/149‑1 (MQ=255)
                                                                                                           ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                          >  1:434539/1‑149 (MQ=255)
                                                                                                             ttAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCt                                        >  1:1440790/1‑149 (MQ=255)
                                                                                                                 tCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAAcc                                    >  1:929858/1‑149 (MQ=255)
                                                                                                                  cAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCg                                   <  2:826994/149‑1 (MQ=255)
                                                                                                                   aGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGACACATACAGGCTAACCGt                                  >  1:14770/1‑149 (MQ=255)
                                                                                                                    gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                 >  1:1078061/1‑149 (MQ=255)
                                                                                                                       tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                              <  2:1187591/149‑1 (MQ=255)
                                                                                                                       tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                              >  1:1110488/1‑149 (MQ=255)
                                                                                                                        ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                             <  2:192261/149‑1 (MQ=255)
                                                                                                                        ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                             <  1:1304499/149‑1 (MQ=255)
                                                                                                                            gcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGTCACGAat                         <  1:414753/149‑1 (MQ=255)
                                                                                                                              aGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAatat                       <  1:524018/149‑1 (MQ=255)
                                                                                                                               ttgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                      <  1:765389/148‑1 (MQ=255)
                                                                                                                               gtgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGACCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                      <  1:139382/149‑1 (MQ=255)
                                                                                                                                 gtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGt                    <  2:1571106/149‑1 (MQ=255)
                                                                                                                                  tAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGtt                   >  2:1212184/1‑149 (MQ=255)
                                                                                                                                   aaCAAAAAACAACGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCTAAGCCCAGAATGCTCAGCAGGCTGACCAAGATGTTGTACAGCGAAACATACAGGCAAACCGAGGCAGGAATATAGTTc                  >  1:285753/1‑149 (MQ=255)
                                                                                                                                   aaCAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTc                  >  2:1477428/1‑149 (MQ=255)
                                                                                                                                     cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                <  1:880107/149‑1 (MQ=255)
                                                                                                                                        aaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtctc             <  1:902819/149‑1 (MQ=255)
                                                                                                                                         aaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa            >  2:833334/1‑149 (MQ=255)
                                                                                                                                           aCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCAcc          >  2:1445315/1‑149 (MQ=255)
                                                                                                                                             ataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGc        >  2:479052/3‑149 (MQ=255)
                                                                                                                                             ataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGc        >  1:1342150/3‑149 (MQ=255)
                                                                                                                                               aGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCa      >  1:1309651/1‑149 (MQ=255)
                                                                                                                                                gCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCat     <  2:46852/149‑1 (MQ=255)
                                                                                                                                                 ccccGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCatg    >  1:1191776/1‑149 (MQ=255)
                                                                                                                                                   ccGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGATGCCCAGAATGCTCAGCAGGCAGCCGAAGATGTTGTACAGCGAAACATACAGGCTAGCCGTGGCACGAATATAGTTCGTCTCACCGCCatgaa  >  1:806025/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCAATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAA  >  NC_000913/1030573‑1030868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: