Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,030,724 A→T 5.1% intergenic (‑56/+35) tusE ← / ← yccA mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,030,7240AT 5.1% 416.7 / 7.2 132intergenic (‑56/+35)tusE/yccAmnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (69/56);  new base T (5/2);  total (74/58)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGAT  >  NC_000913/1030577‑1030872
                                                                                                                                                   |                                                                                                                                                    
aTCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCAt                                                                                                                                                     >  2:1353728/1‑149 (MQ=255)
 tCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATa                                                                                                                                                    >  1:826994/1‑149 (MQ=255)
    ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAATATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAg                                                                                                                                                 >  2:112273/1‑149 (MQ=255)
    ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGAATGGCCGCAGTGTAACAAAAAACAAAGCCCCGCATaaac                                                                                                                                                 >  2:475010/1‑148 (MQ=255)
     aacaGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGc                                                                                                                                                >  2:1472581/2‑149 (MQ=255)
       cagccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGa                                                                                                                                              <  1:412655/149‑1 (MQ=255)
          ccagtGTCTAACTACACTGGCTGCTTTCTTTTTGATAGCCTTCGGTATCCGTTTCTATCTCATTACCTTAGAATATCATCAAAATTATTACAACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                           <  2:310142/149‑1 (MQ=255)
          ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTAACCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                           >  1:937398/1‑149 (MQ=255)
               ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgt                                                                                                                                      <  1:1464462/149‑2 (MQ=255)
               ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCTCATAAAGCGAGGCTATGa                                                                                                                                      <  2:264919/149‑1 (MQ=255)
                 cTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGaaa                                                                                                                                    <  1:768479/149‑1 (MQ=255)
                    aCTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgtttttt                                                                                                                                 <  2:1207730/149‑7 (MQ=255)
                      tCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGtt                                                                                                                               >  1:343202/1‑149 (MQ=255)
                        cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                             >  1:1531298/1‑149 (MQ=255)
                             gctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGt                                                                                                                        <  2:937398/149‑1 (MQ=255)
                              ctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTg                                                                                                                       <  1:1701/149‑1 (MQ=255)
                                gctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgag                                                                                                                     >  1:1424169/1‑149 (MQ=255)
                                  tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                   <  1:763054/149‑1 (MQ=255)
                                  tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                   >  2:1484781/1‑149 (MQ=255)
                                  tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGACAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                   >  1:1224895/1‑149 (MQ=255)
                                   ttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAtt                                                                                                                  >  1:1187591/1‑149 (MQ=255)
                                      tttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAAt                                                                                                               <  1:1182248/149‑1 (MQ=255)
                                         gagaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgc                                                                                                            >  2:1368993/1‑149 (MQ=255)
                                          agaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgcg                                                                                                           <  2:504764/149‑1 (MQ=255)
                                            attgCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACAACCTTAAACAGACTGGCGGCATTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAATAATCGCGGc                                                                                                         >  2:634706/4‑149 (MQ=255)
                                            aTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGc                                                                                                         <  1:1143368/149‑1 (MQ=255)
                                             tAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCt                                                                                                        >  2:1434972/1‑149 (MQ=255)
                                                ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                     >  2:139382/1‑149 (MQ=255)
                                                ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                     >  2:1312279/1‑149 (MQ=255)
                                                ccTTCGGTATCCGTTTCTACCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                     >  2:927255/1‑149 (MQ=255)
                                                  ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                                   >  1:378103/1‑149 (MQ=255)
                                                   tCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGaa                                                                                                  <  2:1142336/149‑1 (MQ=255)
                                                    cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                                 <  1:280929/149‑1 (MQ=255)
                                                    cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                                 >  2:389684/1‑149 (MQ=255)
                                                     ggTATCAGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTTGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGc                                                                                                <  2:842983/149‑1 (MQ=255)
                                                      gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                               >  1:746709/1‑149 (MQ=255)
                                                        aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                             <  1:1250670/149‑1 (MQ=255)
                                                        aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                             <  1:1437955/149‑1 (MQ=255)
                                                           cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                          >  1:1438923/1‑149 (MQ=255)
                                                           cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                          <  2:638021/149‑1 (MQ=255)
                                                               tCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCt                                                                                      <  1:1368993/149‑1 (MQ=255)
                                                                  aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                   <  1:1298104/149‑1 (MQ=255)
                                                                  aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                   >  1:937899/1‑149 (MQ=255)
                                                                  aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                   >  2:727147/1‑149 (MQ=255)
                                                                       tttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGc                                                                              >  2:524018/1‑149 (MQ=255)
                                                                        ttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCt                                                                             <  1:1273162/149‑1 (MQ=255)
                                                                          aCCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGa                                                                           <  1:1353728/149‑1 (MQ=255)
                                                                           ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                          >  2:1067233/1‑149 (MQ=255)
                                                                           ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                          <  1:1347028/149‑1 (MQ=255)
                                                                           ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                          >  2:1347028/1‑149 (MQ=255)
                                                                           ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                          >  2:109961/1‑149 (MQ=255)
                                                                            cTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACg                                                                         <  2:1424169/149‑1 (MQ=255)
                                                                                 aaGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAt                                                                    >  1:713925/1‑149 (MQ=255)
                                                                                  aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                   >  1:192261/1‑149 (MQ=255)
                                                                                  aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                   <  2:746709/149‑1 (MQ=255)
                                                                                  aGATAAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                   <  2:4790/149‑1 (MQ=255)
                                                                                   gATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgt                                                                  <  1:109961/149‑1 (MQ=255)
                                                                                     tCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgttg                                                                <  1:1472581/149‑1 (MQ=255)
                                                                                       aGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTa                                                              >  1:754127/1‑149 (MQ=255)
                                                                                        gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                             <  1:901630/149‑1 (MQ=255)
                                                                                        gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                             >  1:1333798/1‑149 (MQ=255)
                                                                                           tAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGc                                                          >  2:603686/1‑149 (MQ=255)
                                                                                            aaTTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCg                                                         >  1:1536122/1‑149 (MQ=255)
                                                                                             attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCTGGGTGACGATGATGTTGTACAGCGa                                                        >  2:1039137/1‑149 (MQ=255)
                                                                                             attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGAGAGATTAATCGCGGCTAGCGAAGCCCAGAAAGCTCAGCAGGATCACGCAGATGTTGTTCAGCGa                                                        >  1:328978/1‑149 (MQ=255)
                                                                                              ttattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAACCCCAGAATGCACAGCAGGCAGACGAAGATGTTGTACAGTGaa                                                       >  2:1068722/1‑149 (MQ=255)
                                                                                               tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                      <  1:335522/149‑1 (MQ=255)
                                                                                               tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                      <  2:1369369/149‑1 (MQ=255)
                                                                                                    ctacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATAc                                                 >  1:453638/1‑149 (MQ=255)
                                                                                                     tacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACa                                                >  1:84855/1‑149 (MQ=255)
                                                                                                      acCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCTAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAg                                               <  1:1240820/149‑1 (MQ=255)
                                                                                                       ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                              <  2:868819/149‑1 (MQ=255)
                                                                                                       ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                              >  1:210535/1‑149 (MQ=255)
                                                                                                             tCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAAcc                                        >  1:929858/1‑149 (MQ=255)
                                                                                                               aGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGACACATACAGGCTAACCGt                                      >  1:14770/1‑149 (MQ=255)
                                                                                                                gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                     >  1:1078061/1‑149 (MQ=255)
                                                                                                                gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                     <  1:1119817/149‑1 (MQ=255)
                                                                                                                   tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                  >  1:1110488/1‑149 (MQ=255)
                                                                                                                   tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                  <  2:1187591/149‑1 (MQ=255)
                                                                                                                    ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                                 <  2:192261/149‑1 (MQ=255)
                                                                                                                        gcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGTCACGAat                             <  1:414753/149‑1 (MQ=255)
                                                                                                                          aGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAatat                           <  1:524018/149‑1 (MQ=255)
                                                                                                                           ttgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                          <  1:765389/148‑1 (MQ=255)
                                                                                                                            tgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCAGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAg                         <  2:377591/149‑1 (MQ=255)
                                                                                                                             gtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGt                        <  2:1571106/149‑1 (MQ=255)
                                                                                                                              tAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGtt                       >  2:1212184/1‑149 (MQ=255)
                                                                                                                               aaCAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTc                      >  2:1477428/1‑149 (MQ=255)
                                                                                                                                 cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGCCGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                    >  2:814116/1‑149 (MQ=255)
                                                                                                                                 cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                    <  1:880107/149‑1 (MQ=255)
                                                                                                                                 aaaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                    <  1:1312279/148‑1 (MQ=255)
                                                                                                                                  aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                   >  2:506726/1‑149 (MQ=255)
                                                                                                                                  aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                   >  1:460887/1‑149 (MQ=255)
                                                                                                                                    aaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtctc                 <  1:902819/149‑1 (MQ=255)
                                                                                                                                     tttcataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa                >  1:1240671/7‑149 (MQ=255)
                                                                                                                                     aaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa                >  2:833334/1‑149 (MQ=255)
                                                                                                                                       aCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCAcc              >  2:1445315/1‑149 (MQ=255)
                                                                                                                                           aGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCa          >  1:1309651/1‑149 (MQ=255)
                                                                                                                                            gCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCat         <  2:46852/149‑1 (MQ=255)
                                                                                                                                             ccccGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCatg        >  1:1191776/1‑149 (MQ=255)
                                                                                                                                                   aTAAAGCGAGGCTATGAAAGAGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATTCTCAGCAGGCTGACGAAGATGTTGTACAGTGAAACATACAGGGTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAatgat  <  1:1068722/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGAT  >  NC_000913/1030577‑1030872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: