Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,030,726 A→T 5.2% intergenic (‑58/+33) tusE ← / ← yccA mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,030,7260AT 5.2% 407.1 / 7.6 132intergenic (‑58/+33)tusE/yccAmnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (66/59);  new base T (5/2);  total (71/61)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 134 total aligned reads displayed.

TCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATG  >  NC_000913/1030578‑1030873
                                                                                                                                                    |                                                                                                                                                   
tCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATa                                                                                                                                                     >  1:826994/1‑149 (MQ=255)
   ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAATATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAg                                                                                                                                                  >  2:112273/1‑149 (MQ=255)
   ccacAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGAATGGCCGCAGTGTAACAAAAAACAAAGCCCCGCATaaac                                                                                                                                                  >  2:475010/1‑148 (MQ=255)
    aacaGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGc                                                                                                                                                 >  2:1472581/2‑149 (MQ=255)
      cagccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGa                                                                                                                                               <  1:412655/149‑1 (MQ=255)
         ccagtGTCTAACTACACTGGCTGCTTTCTTTTTGATAGCCTTCGGTATCCGTTTCTATCTCATTACCTTAGAATATCATCAAAATTATTACAACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                            <  2:310142/149‑1 (MQ=255)
         ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTAACCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                            >  1:937398/1‑149 (MQ=255)
         ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCATCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTATAAGCGGGGc                                                                                                                                            >  1:190578/1‑149 (MQ=255)
           agcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTa                                                                                                                                          >  2:1304499/1‑149 (MQ=255)
              ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgt                                                                                                                                       <  1:1464462/149‑2 (MQ=255)
              ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCTCATAAAGCGAGGCTATGa                                                                                                                                       <  2:264919/149‑1 (MQ=255)
                  cACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAgt                                                                                                                                   <  2:1158712/149‑1 (MQ=255)
                   aCTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgtttttt                                                                                                                                  <  2:1207730/149‑7 (MQ=255)
                    cTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAGAACAAAGCCCCGCATAAAGCGAGGCTATGAAAgtgt                                                                                                                                 <  1:1159606/149‑1 (MQ=255)
                       cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                              >  1:1531298/1‑149 (MQ=255)
                       cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                              <  1:952369/149‑1 (MQ=255)
                            gctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGt                                                                                                                         <  2:937398/149‑1 (MQ=255)
                               gctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgag                                                                                                                      >  1:1424169/1‑149 (MQ=255)
                                ctttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgaga                                                                                                                     <  1:121423/149‑1 (MQ=255)
                                 tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                    <  1:763054/149‑1 (MQ=255)
                                 tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                    >  2:1484781/1‑149 (MQ=255)
                                 tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGACAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                    >  1:1224895/1‑149 (MQ=255)
                                  ttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAtt                                                                                                                   >  1:1187591/1‑149 (MQ=255)
                                     tttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAAt                                                                                                                <  1:1182248/149‑1 (MQ=255)
                                        gagaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgc                                                                                                             >  2:1368993/1‑149 (MQ=255)
                                         agaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgcg                                                                                                            <  2:504764/149‑1 (MQ=255)
                                           attgCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACAACCTTAAACAGACTGGCGGCATTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAATAATCGCGGc                                                                                                          >  2:634706/4‑149 (MQ=255)
                                            tAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCt                                                                                                         >  2:1434972/1‑149 (MQ=255)
                                               ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                      >  2:1312279/1‑149 (MQ=255)
                                               ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                      >  2:139382/1‑149 (MQ=255)
                                               ccTTCGGTATCCGTTTCTACCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                      >  2:927255/1‑149 (MQ=255)
                                                 ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                                    >  1:377591/1‑149 (MQ=255)
                                                 ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                                    >  1:378103/1‑149 (MQ=255)
                                                   cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                                  >  2:389684/1‑149 (MQ=255)
                                                    ggTATCAGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTTGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGc                                                                                                 <  2:842983/149‑1 (MQ=255)
                                                     gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                                >  1:746709/1‑149 (MQ=255)
                                                     gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                                <  1:525394/149‑1 (MQ=255)
                                                       aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                              <  1:1250670/149‑1 (MQ=255)
                                                       aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                              <  1:1437955/149‑1 (MQ=255)
                                                          cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                           <  2:638021/149‑1 (MQ=255)
                                                                tATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGGGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTca                                                                                     <  1:1503375/149‑1 (MQ=255)
                                                                 aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                    >  2:727147/1‑149 (MQ=255)
                                                                     ctTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGATAGTGTGAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGCATGCTCAGCAgg                                                                                >  1:91999/1‑149 (MQ=255)
                                                                      tttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGc                                                                               >  2:524018/1‑149 (MQ=255)
                                                                       ttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCt                                                                              <  1:1273162/149‑1 (MQ=255)
                                                                         aCCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGa                                                                            <  1:1353728/149‑1 (MQ=255)
                                                                          ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                           >  2:1347028/1‑149 (MQ=255)
                                                                          ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                           <  1:1347028/149‑1 (MQ=255)
                                                                          ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                           >  2:109961/1‑149 (MQ=255)
                                                                          ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                           >  2:1067233/1‑149 (MQ=255)
                                                                           cTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACg                                                                          <  2:1424169/149‑1 (MQ=255)
                                                                            ttCGAAGATCAGCATAATTATTACTACATTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACTCATAAAGCGAGTCTATGAAAGTGTTAGAGGGTGAGATTAATCGCGGCTAGCTAAGCCCAGAATGCTCAGCAGGCTGACGa                                                                         >  1:124956/1‑149 (MQ=255)
                                                                                aaGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAt                                                                     >  1:713925/1‑149 (MQ=255)
                                                                                 aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                    <  2:746709/149‑1 (MQ=255)
                                                                                 aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                    >  1:192261/1‑149 (MQ=255)
                                                                                 aGATAAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                    <  2:4790/149‑1 (MQ=255)
                                                                                  gATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgt                                                                   >  1:868819/1‑149 (MQ=255)
                                                                                  gATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgt                                                                   <  1:109961/149‑1 (MQ=255)
                                                                                    tCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgttg                                                                 <  1:1472581/149‑1 (MQ=255)
                                                                                       gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                              <  1:901630/149‑1 (MQ=255)
                                                                                          tAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGc                                                           >  2:603686/1‑149 (MQ=255)
                                                                                           aaTTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCg                                                          >  1:1536122/1‑149 (MQ=255)
                                                                                            attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCTGGGTGACGATGATGTTGTACAGCGa                                                         >  2:1039137/1‑149 (MQ=255)
                                                                                            attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGAGAGATTAATCGCGGCTAGCGAAGCCCAGAAAGCTCAGCAGGATCACGCAGATGTTGTTCAGCGa                                                         >  1:328978/1‑149 (MQ=255)
                                                                                              tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                       <  1:335522/149‑1 (MQ=255)
                                                                                              tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                       <  2:1369369/149‑1 (MQ=255)
                                                                                                   ctacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATAc                                                  >  1:453638/1‑149 (MQ=255)
                                                                                                      ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                               >  1:210535/1‑149 (MQ=255)
                                                                                                      ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                               <  2:868819/149‑1 (MQ=255)
                                                                                                      ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                               >  1:434539/1‑149 (MQ=255)
                                                                                                        ttAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCt                                             >  1:1440790/1‑149 (MQ=255)
                                                                                                            tCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAAcc                                         >  1:929858/1‑149 (MQ=255)
                                                                                                               gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                      >  1:1078061/1‑149 (MQ=255)
                                                                                                               gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                      <  1:1119817/149‑1 (MQ=255)
                                                                                                                  tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                   >  1:1110488/1‑149 (MQ=255)
                                                                                                                  tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                   <  2:1187591/149‑1 (MQ=255)
                                                                                                                   ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                                  <  2:192261/149‑1 (MQ=255)
                                                                                                                   ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                                  <  1:1304499/149‑1 (MQ=255)
                                                                                                                    gcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACg                                 >  2:1077988/1‑149 (MQ=255)
                                                                                                                          ttgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                           <  1:765389/148‑1 (MQ=255)
                                                                                                                          gtgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGACCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                           <  1:139382/149‑1 (MQ=255)
                                                                                                                           tgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCAGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAg                          <  2:377591/149‑1 (MQ=255)
                                                                                                                            gtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGt                         <  2:1571106/149‑1 (MQ=255)
                                                                                                                              aaCAAAAAACAACGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCTAAGCCCAGAATGCTCAGCAGGCTGACCAAGATGTTGTACAGCGAAACATACAGGCAAACCGAGGCAGGAATATAGTTc                       >  1:285753/1‑149 (MQ=255)
                                                                                                                              aaCAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTc                       >  2:1477428/1‑149 (MQ=255)
                                                                                                                                cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGCCGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                     >  2:814116/1‑149 (MQ=255)
                                                                                                                                cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                     <  1:880107/149‑1 (MQ=255)
                                                                                                                                aaaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                     <  1:1312279/148‑1 (MQ=255)
                                                                                                                                 aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                    >  2:506726/1‑149 (MQ=255)
                                                                                                                                 aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                    >  1:460887/1‑149 (MQ=255)
                                                                                                                                    tttcataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa                 >  1:1240671/7‑149 (MQ=255)
                                                                                                                                    aaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa                 >  2:833334/1‑149 (MQ=255)
                                                                                                                                      aCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCAcc               >  2:1445315/1‑149 (MQ=255)
                                                                                                                                        ataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGc             >  1:1342150/3‑149 (MQ=255)
                                                                                                                                          aGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCa           >  1:1309651/1‑149 (MQ=255)
                                                                                                                                           gCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCat          <  2:46852/149‑1 (MQ=255)
                                                                                                                                              ccGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGATGCCCAGAATGCTCAGCAGGCAGCCGAAGATGTTGTACAGCGAAACATACAGGCTAGCCGTGGCACGAATATAGTTCGTCTCACCGCCatgaa       >  1:806025/1‑149 (MQ=255)
                                                                                                                                               cGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCatgaat      >  1:761356/1‑149 (MQ=255)
                                                                                                                                                  aTAAAGCGAGGCTATGAAAGAGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATTCTCAGCAGGCTGACGAAGATGTTGTACAGTGAAACATACAGGGTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAatgat   <  1:1068722/149‑1 (MQ=255)
                                                                                                                                                   tAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAatgatg  <  1:112273/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCACCACAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATG  >  NC_000913/1030578‑1030873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: